FastQCFastQC Report
Mon 15 Aug 2016
SRR2921694_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921694_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148527
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21550.042.063081
GTATCAA39750.041.5486681
TCAACGC45000.035.754994
CAACGCA47000.034.229695
ATCAACG47600.034.000873
TATCAAC50950.032.3252332
AACGCAG50400.032.0014846
ACGCAGA61150.026.0691367
GTACATG79050.025.4553411
CGCAGAG63650.024.6744758
TACATGG82550.023.5159532
ACATGGG83850.021.7355543
GCAGAGT74850.020.2223229
GAGTACA60600.019.7352621
CATGGGG74100.019.66394
GTATTAC7250.018.3288421
AGAGTAC61250.017.97344210-11
AGTACAT62850.017.6712362
TAAGGTA9300.017.3518374
GTATATA11650.017.109541
Produced by FastQC (version 0.11.5)