##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921694_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3148527
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.26429565317369	31.0	31.0	34.0	26.0	34.0
2	30.32351953786644	31.0	31.0	34.0	26.0	34.0
3	30.384622714050092	33.0	31.0	34.0	26.0	34.0
4	33.82676756464213	37.0	35.0	37.0	29.0	37.0
5	33.81925770368176	37.0	35.0	37.0	29.0	37.0
6	33.862592888674605	37.0	35.0	37.0	30.0	37.0
7	33.77251076455752	37.0	35.0	37.0	29.0	37.0
8	33.74312083078849	37.0	35.0	37.0	29.0	37.0
9	35.29086998459915	39.0	37.0	39.0	29.0	39.0
10-11	35.18642638287682	39.0	35.0	39.0	28.0	39.0
12-13	35.07813637932912	39.0	35.0	39.0	27.5	39.0
14-15	36.21854362373262	40.0	37.0	41.0	27.0	41.0
16-17	36.15521353318552	40.0	36.0	41.0	27.0	41.0
18-19	36.12104549206661	40.0	36.5	41.0	27.0	41.0
20-21	36.04441013210304	40.0	36.0	41.0	27.0	41.0
22-23	35.945502928829896	40.0	36.0	41.0	27.0	41.0
24-25	35.841448397933384	40.0	36.0	41.0	27.0	41.0
26-27	35.6948363472824	40.0	36.0	41.0	26.5	41.0
28-29	35.56307965597881	40.0	36.0	41.0	25.5	41.0
30-31	35.424634916581624	40.0	36.0	41.0	25.0	41.0
32-33	35.25427652359342	39.5	35.0	41.0	25.0	41.0
34-35	35.087717843931465	39.0	35.0	41.0	24.0	41.0
36-37	35.05788262257239	39.0	35.0	41.0	24.0	41.0
38-39	35.242047471722486	39.5	35.0	41.0	24.5	41.0
40-41	35.30558114953437	40.0	35.0	41.0	24.0	41.0
42-43	35.15143192356299	40.0	35.0	41.0	24.0	41.0
44-45	35.034071805641176	40.0	35.0	41.0	23.0	41.0
46-47	34.88126209494154	40.0	35.0	41.0	22.5	41.0
48-49	34.72218421503135	39.0	35.0	41.0	21.0	41.0
50-51	33.041370456724685	37.0	32.5	39.5	17.0	40.0
52-53	33.159146165810235	37.5	33.0	39.5	16.5	40.0
54-55	34.07224044767601	39.0	34.0	40.5	18.0	41.0
56-57	34.09640238117697	39.0	34.0	41.0	18.0	41.0
58-59	33.91046813319372	38.0	34.0	41.0	16.0	41.0
60-61	33.65821334865478	38.0	34.0	40.0	13.5	41.0
62-63	33.36997554729561	37.0	34.0	40.0	12.5	41.0
64-65	33.08387985874029	37.0	33.0	40.0	9.0	41.0
66-67	32.742684436245895	36.0	33.0	39.5	7.5	41.0
68-69	32.38929759852782	36.0	33.0	39.0	4.5	41.0
70-71	31.986183697964158	35.0	32.0	39.0	2.0	40.5
72-73	31.633634712359143	35.0	32.0	37.5	2.0	40.0
74-75	31.247598003764935	35.0	32.0	37.0	2.0	39.0
76-77	30.88002802580381	35.0	32.0	36.5	2.0	39.0
78-79	30.546353739383527	35.0	31.0	36.0	2.0	38.5
80-81	30.247992791549827	35.0	31.0	36.0	2.0	37.0
82-83	29.94746924514225	35.0	31.0	35.0	2.0	37.0
84-85	29.698965738581883	35.0	31.0	35.0	2.0	36.5
86-87	29.481307608287942	35.0	31.0	35.0	2.0	36.0
88-89	29.27852913441746	35.0	31.0	35.0	2.0	36.0
90-91	29.072207575161336	34.5	30.0	35.0	2.0	35.5
92-93	28.898947666639035	34.0	30.0	35.0	2.0	35.0
94-95	28.689127010821252	34.0	30.0	35.0	2.0	35.0
96-97	28.47955170783036	34.0	30.0	35.0	2.0	35.0
98-99	28.234612566447737	34.0	30.0	35.0	2.0	35.0
100-101	27.193617682173283	33.0	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	117812.0
3	19838.0
4	13102.0
5	9911.0
6	8869.0
7	9082.0
8	9538.0
9	10144.0
10	11346.0
11	12048.0
12	12035.0
13	11885.0
14	12779.0
15	13285.0
16	13128.0
17	13765.0
18	13270.0
19	12584.0
20	12779.0
21	13136.0
22	14364.0
23	16683.0
24	19740.0
25	23246.0
26	27488.0
27	32766.0
28	38658.0
29	45813.0
30	54979.0
31	67466.0
32	83745.0
33	108544.0
34	150244.0
35	218508.0
36	368022.0
37	673486.0
38	726299.0
39	128124.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.40700243295394	17.563760172264324	12.857920065895682	25.17131732888605
2	15.771023433040193	19.13671795041314	39.80072825378017	25.2915303627665
3	19.573894354634167	23.19218806316829	28.74452286554648	28.489394716651063
4	13.425610001452517	15.41260218869179	36.295662505009915	34.86612530484578
5	14.648813487138495	36.690349780145915	34.16031947571911	14.500517256996476
6	34.02352776749852	34.70690347961292	17.085504551020506	14.184064201868054
7	29.513785320963947	31.13733861271658	20.66491004877553	18.683966017543945
8	26.62815933559765	34.94621299045862	19.631591386454517	18.794036287489217
9	26.6407014793296	15.962999925674598	19.145588215675797	38.250710379320005
10-11	25.85009660504538	25.019221743719083	27.62253117130739	21.508150479928148
12-13	26.42137153297043	22.284690627319566	27.452198168665333	23.841739671044678
14-15	23.45680679467313	23.88309430751355	24.6153753277821	28.04472357003122
16-17	23.900090574328928	27.074052059112535	25.330599477521776	23.695257889036757
18-19	23.869777822035598	26.46429195931224	26.518989543137	23.14694067551516
20-21	24.37699230390345	25.65854732425118	26.497727290415863	23.46673308142951
22-23	24.136455256696372	25.511235391005076	26.230818942509572	24.12149040978898
24-25	24.044718133548194	25.69877246581993	26.208280486286036	24.048228914345845
26-27	23.892639473652025	25.835804705993354	26.20705462200824	24.06450119834638
28-29	24.000042564390156	26.043832110162995	26.024281078715056	23.931844246731792
30-31	24.250101130035834	25.78360888190918	25.93266351658715	24.03362647146783
32-33	23.949597715570775	25.807316399165252	25.94092946284785	24.30215642241612
34-35	24.284678376838333	25.844912326172174	25.954556224895768	23.91585307209372
36-37	24.376332177337158	25.724159769491038	25.913614403761017	23.985893649410787
38-39	24.180990056870066	25.79603005331271	25.963305229155715	24.05967466066151
40-41	24.404529840325527	25.662097135476756	25.970243920953457	23.96312910324426
42-43	24.213625055509663	25.639861269615405	25.877323248481144	24.269190426393788
44-45	24.125529570416457	25.75267221729393	25.99125636976247	24.13054184252714
46-47	24.345792555846742	25.728815107459063	25.76574896402999	24.159643372664206
48-49	24.169122363987896	25.77060251409722	25.793980508577206	24.26629461333768
50-51	24.165208792880506	25.848635306016643	25.935646263223106	24.050509637879745
52-53	24.38107278171596	25.71344045363807	25.87488796545262	24.03059879919335
54-55	23.953421758260994	25.582832727301664	26.116198948130588	24.34754656630676
56-57	23.970532864875565	25.759506623453465	26.071558950343114	24.198401561327852
58-59	24.163658821467923	25.626443212641277	26.028159447015902	24.1817385188749
60-61	24.08556446261827	25.65851598011087	26.138189080457845	24.117730476813016
62-63	24.115297562090333	25.651083419041115	26.16335683182661	24.070262187041937
64-65	23.920687200635143	25.54034267414543	26.111243459307065	24.42772666591236
66-67	23.87340214276655	25.733010466542726	26.01239728273764	24.38119010795308
68-69	24.01514097920534	25.516015684547234	26.139814098461557	24.32902923778587
70-71	23.954448548967157	25.36809835961761	26.120039071195144	24.557414020220094
72-73	23.968657729388074	25.54242289946238	26.00440949593144	24.48450987521811
74-75	23.921651249049013	25.65576127407821	26.067488285416534	24.35509919145624
76-77	23.967666067271345	25.358606907891858	26.170599015031343	24.50312800980545
78-79	23.89708006254451	25.410848210200488	26.17954094238505	24.512530784869956
80-81	24.022131364629544	25.559953065870765	26.03453083697075	24.383384732528942
82-83	23.982678622133317	25.595619328230786	26.10408106356456	24.31762098607134
84-85	23.952301754665182	25.579723869016828	26.138527036167584	24.329447340150402
86-87	23.881069339341142	25.615201541790437	26.11906596687354	24.384663151994886
88-89	23.89336906437764	25.446462964059485	26.138147091950138	24.52202087961274
90-91	23.794661362242785	25.466280763037304	26.19656761835146	24.54249025636846
92-93	23.91526779696845	25.37440702770658	26.34530414969978	24.36502102562519
94-95	23.848267169639044	25.251471596933584	26.3899087585228	24.510352474904572
96-97	23.84009559191194	25.1443597381156	26.37418547167862	24.641359198293838
98-99	23.74704440170604	25.124248942956758	26.400835001287774	24.727871654049427
100-101	23.872202145490743	24.992450331779462	26.503959974102685	24.631387548627107
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	49.0
1	37.5
2	49.0
3	76.5
4	70.5
5	74.5
6	119.0
7	249.0
8	415.0
9	456.5
10	407.0
11	403.5
12	456.0
13	537.5
14	629.0
15	724.0
16	790.5
17	848.5
18	997.5
19	1151.0
20	1231.0
21	1320.5
22	1450.0
23	1603.5
24	1887.0
25	2289.5
26	2842.5
27	3682.0
28	5024.0
29	7217.0
30	10517.5
31	15306.0
32	22276.0
33	29047.5
34	35022.5
35	41880.0
36	50837.5
37	60374.0
38	69067.5
39	77578.5
40	85325.5
41	92348.0
42	99919.0
43	108308.5
44	115828.0
45	124274.0
46	130396.5
47	133973.0
48	138160.5
49	140046.0
50	138913.0
51	137803.0
52	137292.5
53	136187.0
54	133913.5
55	127763.0
56	118314.0
57	106665.0
58	96471.5
59	85198.5
60	70599.5
61	58235.5
62	47361.0
63	36128.5
64	26124.5
65	18849.0
66	13950.5
67	10781.5
68	8148.5
69	6024.0
70	4534.5
71	3173.5
72	2160.5
73	1618.5
74	1221.0
75	896.5
76	665.0
77	474.5
78	317.5
79	217.0
80	164.0
81	132.5
82	102.0
83	82.0
84	66.0
85	51.0
86	50.0
87	53.5
88	50.5
89	36.5
90	32.0
91	30.0
92	25.0
93	21.5
94	15.5
95	16.0
96	16.0
97	10.5
98	9.5
99	7.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05510513328931274
2	0.07530505534810406
3	0.07193840167163884
4	0.0719066407878986
5	0.06739659529678482
6	0.010544613401758982
7	0.013720701775782771
8	0.01762729047583203
9	0.006638024701709721
10-11	0.01268847305422504
12-13	0.022661390548659736
14-15	0.039319974070414515
16-17	0.026552098806838884
18-19	0.009766471750123153
20-21	0.02008875896570047
22-23	0.036556777185013815
24-25	0.034269993555716693
26-27	0.020565172221804037
28-29	0.011290994169654572
30-31	0.011195711518433859
32-33	0.00816254712124114
34-35	0.004859415212256398
36-37	5.081741398438063E-4
38-39	0.0038748278163090234
40-41	8.734243028565422E-4
42-43	0.22810666702238855
44-45	0.19817203409721434
46-47	0.20401603670541812
48-49	0.21227386647787996
50-51	0.2030790906350811
52-53	0.21086050715143936
54-55	0.2065092660790268
56-57	0.20917718031320678
58-59	0.2185625214584471
60-61	0.22272319722841824
62-63	0.2081131907079088
64-65	0.20843079954531119
66-67	0.20541351558998858
68-69	0.20091935054074492
70-71	0.19242331414023128
72-73	0.20468301526396313
74-75	0.2031584928444317
76-77	0.1967110334451634
78-79	0.21484649806083922
80-81	0.22559755720690977
82-83	0.22521642660202693
84-85	0.21921361957512195
86-87	0.23831779114487506
88-89	0.23125099451267211
90-91	0.23823838893552446
92-93	0.2337601043281509
94-95	0.23714263844648625
96-97	0.243780663148196
98-99	0.23674562739973326
100-101	0.2433042498920924
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3148527.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.34738252592298
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.10390753697777	48.52817674966217
2	11.800581034402864	14.478695174913286
3	3.8153829509362462	7.021912721219121
4	1.7186303030311705	4.217338824827846
5	0.930474479589576	2.8541086914995413
6	0.5799656786189009	2.134762580288413
7	0.38869261884893	1.6691692341469666
8	0.27553881290284615	1.3522867964711702
9	0.20432009679941426	1.128105282247855
>10	1.1125736896996028	12.006142090548872
>50	0.05249806017746724	2.198015899325553
>100	0.01593054403632453	1.6226241493207685
>500	0.0010373751584822012	0.4339628691353161
>1k	4.6681882037226304E-4	0.35469893639307926
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	3.17608837402379E-5
8	0.0	0.0	0.0	0.0	3.17608837402379E-5
9	0.0	0.0	0.0	0.0	6.35217674804758E-5
10-11	0.0	0.0	0.0	0.0	6.35217674804758E-5
12-13	0.0	0.0	0.0	0.0	1.4292397683107055E-4
14-15	0.0	0.0	0.0	0.0	1.588044187011895E-4
16-17	0.0	0.0	0.0	1.588044187011895E-5	1.588044187011895E-4
18-19	0.0	0.0	0.0	3.17608837402379E-5	1.9056530244142736E-4
20-21	0.0	0.0	0.0	4.764132561035685E-5	2.540870699219032E-4
22-23	0.0	0.0	0.0	1.1116309309083263E-4	2.6996751179202215E-4
24-25	0.0	0.0	0.0	2.3820662805178422E-4	3.0172839553226E-4
26-27	0.0	0.0	0.0	3.652501630127358E-4	3.17608837402379E-4
28-29	0.0	0.0	0.0	0.0012545549077393968	3.17608837402379E-4
30-31	0.0	0.0	0.0	0.0059869265850348435	3.17608837402379E-4
32-33	0.0	0.0	0.0	0.01996171543073952	3.4936972114261685E-4
34-35	0.0	0.0	0.0	0.03801777783706476	3.4936972114261685E-4
36-37	0.0	0.0	0.0	0.06782536722727803	3.4936972114261685E-4
38-39	0.0	0.0	0.0	0.11778523735067223	3.4936972114261685E-4
40-41	0.0	0.0	0.0	0.1779244707128127	3.4936972114261685E-4
42-43	0.0	0.0	0.0	0.24176384703069087	3.4936972114261685E-4
44-45	0.0	0.0	0.0	0.31832345728653433	3.652501630127358E-4
46-47	0.0	0.0	0.0	0.41246271669259943	3.970110467529737E-4
48-49	3.17608837402379E-5	0.0	0.0	0.5153679800109703	4.1289148862309264E-4
50-51	3.17608837402379E-5	0.0	0.0	0.6268010406135949	4.1289148862309264E-4
52-53	3.17608837402379E-5	0.0	0.0	0.7396633409845302	4.446523723633305E-4
54-55	3.17608837402379E-5	0.0	0.0	0.8673262131784165	4.922936979736874E-4
56-57	3.17608837402379E-5	0.0	0.0	1.0171899431067288	5.240545817139252E-4
58-59	3.17608837402379E-5	0.0	0.0	1.1805202877409022	5.399350235840442E-4
60-61	3.17608837402379E-5	0.0	0.0	1.3593181827565717	5.875763491944011E-4
62-63	3.17608837402379E-5	0.0	0.0	1.5443412109853274	6.0345679106452E-4
64-65	3.17608837402379E-5	0.0	0.0	1.743339028059788	6.19337232934639E-4
66-67	3.17608837402379E-5	0.0	0.0	1.9770991323879388	6.35217674804758E-4
68-69	3.17608837402379E-5	0.0	0.0	2.2345846168700474	6.35217674804758E-4
70-71	3.17608837402379E-5	0.0	0.0	2.5077440974779632	6.35217674804758E-4
72-73	4.764132561035685E-5	0.0	0.0	2.7950689322340256	6.987394422852337E-4
74-75	6.35217674804758E-5	0.0	0.0	3.0933353914385995	7.305003260254716E-4
76-77	6.35217674804758E-5	0.0	0.0	3.4236327018952037	7.305003260254716E-4
78-79	6.35217674804758E-5	0.0	0.0	3.798029999425128	7.305003260254716E-4
80-81	6.35217674804758E-5	0.0	0.0	4.191960240455298	7.622612097657095E-4
82-83	6.35217674804758E-5	0.0	0.0	4.604200630961717	8.575438609864232E-4
84-85	6.35217674804758E-5	0.0	0.0	5.040404608250144	8.575438609864232E-4
86-87	6.35217674804758E-5	0.0	0.0	5.502985999484839	9.051851865967801E-4
88-89	6.35217674804758E-5	0.0	0.0	6.0065548111863105	9.687069540772558E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2155	0.0	42.06308	1
GTATCAA	3975	0.0	41.548668	1
TCAACGC	4500	0.0	35.75499	4
CAACGCA	4700	0.0	34.22969	5
ATCAACG	4760	0.0	34.00087	3
TATCAAC	5095	0.0	32.325233	2
AACGCAG	5040	0.0	32.001484	6
ACGCAGA	6115	0.0	26.069136	7
GTACATG	7905	0.0	25.455341	1
CGCAGAG	6365	0.0	24.674475	8
TACATGG	8255	0.0	23.515953	2
ACATGGG	8385	0.0	21.735554	3
GCAGAGT	7485	0.0	20.222322	9
GAGTACA	6060	0.0	19.735262	1
CATGGGG	7410	0.0	19.6639	4
GTATTAC	725	0.0	18.328842	1
AGAGTAC	6125	0.0	17.973442	10-11
AGTACAT	6285	0.0	17.671236	2
TAAGGTA	930	0.0	17.351837	4
GTATATA	1165	0.0	17.10954	1
>>END_MODULE