FastQCFastQC Report
Mon 15 Aug 2016
SRR2921694_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921694_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3148527
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA22450.044.0414771
GTATCAA41400.041.564551
TCAACGC48800.034.234814
ATCAACG50450.033.020543
CAACGCA50800.033.0087285
TATCAAC55300.030.6394712
AACGCAG55500.030.3782986
GTACATG82500.025.5249371
ACGCAGA66600.024.459527
TACATGG86400.023.6207082
CGCAGAG69700.023.0309568
ACATGGG87850.022.4204623
GTATATA9050.020.484761
TATACCG2801.5770638E-920.3582785
CATGGGG74650.019.9638794
TATACTG13550.019.6320925
GCAGAGT81300.018.576539
AGAGTAC68700.017.76660510-11
CTATACT12300.017.7499774
CCTATAC8550.017.2081343
Produced by FastQC (version 0.11.5)