##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921694_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3148527
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.33336795269661	33.0	31.0	34.0	28.0	34.0
2	31.47116635810968	34.0	31.0	34.0	28.0	34.0
3	31.548356739516606	34.0	31.0	34.0	28.0	34.0
4	35.01800206890397	37.0	35.0	37.0	32.0	37.0
5	34.758393051734984	37.0	35.0	37.0	32.0	37.0
6	34.73150492277818	37.0	35.0	37.0	32.0	37.0
7	34.61675475547772	37.0	35.0	37.0	32.0	37.0
8	34.563884000359536	37.0	35.0	37.0	32.0	37.0
9	36.15013719113732	39.0	37.0	39.0	32.0	39.0
10-11	36.03971666750833	39.0	37.0	39.0	31.0	39.0
12-13	35.96069971767751	39.0	37.0	39.0	31.0	39.0
14-15	37.20992594314738	40.0	38.0	41.0	31.0	41.0
16-17	37.17693432516221	40.0	38.0	41.0	31.0	41.0
18-19	37.134399673244026	40.0	38.0	41.0	31.0	41.0
20-21	37.079305815068444	40.0	38.0	41.0	31.0	41.0
22-23	36.98547288938605	40.0	38.0	41.0	31.0	41.0
24-25	36.90821628653653	40.0	37.0	41.0	30.0	41.0
26-27	36.78186037470856	40.0	37.0	41.0	30.0	41.0
28-29	36.66540750643078	40.0	37.0	41.0	30.0	41.0
30-31	36.51227685200095	40.0	37.0	41.0	30.0	41.0
32-33	36.46465807661805	40.0	37.0	41.0	30.0	41.0
34-35	36.59865136935463	40.0	37.0	41.0	30.0	41.0
36-37	36.60279711750923	40.0	37.0	41.0	30.0	41.0
38-39	36.498378606885055	40.0	37.0	41.0	30.0	41.0
40-41	36.42165971579726	40.0	37.0	41.0	30.0	41.0
42-43	36.30268868585215	40.0	37.0	41.0	29.0	41.0
44-45	36.17709567045161	40.0	36.0	41.0	28.5	41.0
46-47	36.02764260875006	40.0	36.0	41.0	28.0	41.0
48-49	35.88965300281687	40.0	36.0	41.0	28.0	41.0
50-51	35.73542342180963	40.0	35.0	41.0	27.5	41.0
52-53	35.56382127261415	39.0	35.0	41.0	27.0	41.0
54-55	35.34824316259635	39.0	35.0	41.0	26.5	41.0
56-57	35.12469815250115	39.0	35.0	41.0	26.0	41.0
58-59	34.88370768298954	38.5	35.0	40.5	26.0	41.0
60-61	34.6111815144034	38.0	34.5	40.0	26.0	41.0
62-63	34.305747735369586	37.5	34.0	40.0	25.5	41.0
64-65	33.967277237895686	37.0	34.0	40.0	24.5	41.0
66-67	33.615480985235315	36.5	34.0	39.0	23.5	41.0
68-69	33.25732334517062	36.0	34.0	39.0	23.0	41.0
70-71	32.86012205072404	35.5	33.0	39.0	22.0	40.0
72-73	32.49779261858005	35.0	33.0	37.5	22.0	40.0
74-75	32.09635807474416	35.0	33.0	37.0	20.5	39.0
76-77	29.408833400507604	33.0	29.0	34.5	14.0	36.5
78-79	30.97010157448229	34.5	31.5	36.0	17.5	37.5
80-81	31.153035371778614	35.0	32.0	36.0	18.0	37.0
82-83	30.977273499639672	35.0	32.0	35.0	18.0	37.0
84-85	30.777779259952354	35.0	32.0	35.0	16.5	36.5
86-87	30.568520295363513	35.0	32.0	35.0	12.0	36.0
88-89	30.3054183750052	35.0	32.0	35.0	7.0	36.0
90-91	30.11588196639254	34.5	31.5	35.0	3.5	35.0
92-93	29.962538990454902	34.0	31.0	35.0	2.0	35.0
94-95	29.856507344545562	34.0	31.0	35.0	2.0	35.0
96-97	29.660797890569143	34.0	31.0	35.0	2.0	35.0
98-99	29.464483550561894	34.0	31.0	35.0	2.0	35.0
100-101	28.363892067624004	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	62991.0
3	21131.0
4	11130.0
5	7866.0
6	6909.0
7	6718.0
8	7141.0
9	7589.0
10	8442.0
11	9094.0
12	9595.0
13	10010.0
14	11038.0
15	11905.0
16	12490.0
17	13312.0
18	12715.0
19	11524.0
20	10914.0
21	10850.0
22	11562.0
23	13142.0
24	15630.0
25	19029.0
26	22562.0
27	27335.0
28	33252.0
29	40021.0
30	48512.0
31	60376.0
32	77390.0
33	102301.0
34	144803.0
35	217481.0
36	386293.0
37	761236.0
38	805400.0
39	98837.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.9553259685206	19.21074021935371	13.560799471896798	26.2731343402289
2	14.883310253706503	20.577922325410032	38.12003735088232	26.418730070001146
3	18.113887673884584	24.423682959159656	28.636142582104906	28.82628678485085
4	13.065869632885901	16.671356969069414	35.290152905975845	34.972620492068835
5	14.308283995847138	37.071450083679935	34.28450161196655	14.335764308506379
6	33.95081197122774	35.41067363644766	16.776482642723447	13.862031749601153
7	29.27181504239919	31.388582661034825	20.69618586723252	18.643416429333463
8	26.523005837332825	35.56612346027206	19.348158678645603	18.56271202374952
9	26.37704119324408	16.06142048691991	19.022171009545417	38.539367310290594
10-11	25.756573365643288	25.422209215199914	27.521704674075586	21.299512745081216
12-13	26.327576131007586	22.501318079605657	27.51378425418927	23.65732153519749
14-15	23.291768823059886	24.167113724959574	24.599323012401065	27.941794439579475
16-17	23.80208646806689	27.342345697265053	25.29624572002173	23.559322114646328
18-19	23.785755655007726	26.709651559384152	26.5350112062349	22.969581579373223
20-21	24.265301283762504	25.8401054337143	26.494397285580508	23.40019599694269
22-23	24.014308913342653	25.676133633283122	26.295073855171005	24.014483598203224
24-25	23.927500286643614	25.93913500009052	26.22177672034604	23.911587992919824
26-27	23.75810521735402	26.073033110100734	26.223536455413566	23.945325217131685
28-29	23.90870375299441	26.251474574430038	26.04902034896832	23.79080132360723
30-31	24.145666084711348	26.022980246555917	25.887164200345108	23.944189468387627
32-33	23.8852642808577	26.00317042688316	25.897743647750676	24.21382164450846
34-35	24.14287474930103	26.062083769826422	26.02690491512923	23.768136565743323
36-37	24.238858211882565	25.947268370074276	25.99572162959222	23.81815178845094
38-39	24.081300265584595	25.98347607737716	25.968866066216385	23.966357590821865
40-41	24.288584429635975	25.844378940761942	25.96915567426047	23.89788095534162
42-43	24.112060445402726	25.87195437943771	25.836426473991175	24.17955870116839
44-45	24.01857507452423	25.953759245054087	25.916231114837984	24.111434565583703
46-47	24.195383917111624	25.899298284508422	25.785400137910138	24.119917660469817
48-49	24.011384676077345	25.992903397329385	25.83664282603731	24.159069100555957
50-51	24.08052404182247	26.024400570312196	25.824905644077038	24.070169743788295
52-53	24.29437613051481	25.88463230378074	25.744611245833276	24.076380319871173
54-55	23.846419550356412	25.77987268065427	26.0261419951356	24.347565773853717
56-57	23.936725904472645	25.879458915703253	25.94543163533254	24.238383544491562
58-59	24.02821456026328	25.795611209585704	25.94873920750186	24.227435022649157
60-61	23.989163577537706	25.850311829840596	26.0025382181937	24.157986374428
62-63	24.026427645623667	25.845150960658735	26.008755209921723	24.119666183795875
64-65	23.790232318798378	25.72413844599598	25.94746355139504	24.538165683810607
66-67	23.783408428390622	25.94292034097473	25.833610752033763	24.440060478600888
68-69	23.9153210827548	25.765126240149428	25.91063456437591	24.408918112719853
70-71	23.820437559494202	25.62969696295508	25.919766456950434	24.63009902060029
72-73	23.82140574105399	25.748466370722074	25.825227264351046	24.60490062387289
74-75	23.753817922808544	25.876303431063263	25.80525051355708	24.564628132571116
76-77	23.790821709779383	25.610098306194594	25.973173708126595	24.625906275899425
78-79	23.70123832366166	25.639721087105443	25.98311709160035	24.675923497632546
80-81	23.853085044660276	25.73403367600925	25.78030930618496	24.632571973145513
82-83	23.773586104521083	25.81719493239782	25.825200924183733	24.584018038897366
84-85	23.73864477213436	25.759209188509057	25.845327230286475	24.656818809070106
86-87	23.64997214242104	25.817460234219787	25.810245550335768	24.722322073023406
88-89	23.573203047630454	25.677853777063262	25.84409801115324	24.90484516415304
90-91	23.44590506143527	25.705908689749947	25.89082850288059	24.957357745934196
92-93	23.570844650930738	25.637128763605944	25.99202531434387	24.800001271119452
94-95	23.437739719478774	25.48609353057975	26.051112838370745	25.025053911570733
96-97	23.389927636144222	25.36461068988909	26.068046261661387	25.177415412305304
98-99	23.24371476884807	25.394935099088705	26.02071086729774	25.34063926476548
100-101	23.167736109915666	25.39471682436495	26.094394101615265	25.34315296410412
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	35.0
1	28.5
2	34.5
3	52.0
4	54.5
5	57.5
6	107.0
7	268.5
8	398.5
9	399.0
10	328.5
11	253.5
12	255.5
13	284.5
14	322.5
15	370.5
16	402.5
17	446.5
18	526.0
19	612.5
20	695.5
21	817.5
22	1002.5
23	1237.5
24	1578.0
25	2057.0
26	2740.5
27	3765.5
28	5364.5
29	7820.0
30	11438.0
31	16496.0
32	23837.0
33	30541.5
34	36070.0
35	43059.5
36	52098.5
37	61040.5
38	69434.0
39	78058.5
40	85994.5
41	93586.5
42	100752.0
43	108356.0
44	116102.0
45	124411.0
46	130754.5
47	134802.0
48	139103.0
49	140696.5
50	139430.0
51	138646.5
52	138231.0
53	137189.5
54	134615.5
55	128034.5
56	118380.5
57	106542.0
58	95695.0
59	84048.0
60	69263.0
61	56924.5
62	46320.5
63	34949.5
64	24803.0
65	17680.5
66	13028.5
67	9983.0
68	7468.0
69	5471.0
70	4033.0
71	2815.0
72	1892.5
73	1371.5
74	996.5
75	657.0
76	428.0
77	270.0
78	167.0
79	92.5
80	51.0
81	36.0
82	25.0
83	15.5
84	11.5
85	10.0
86	5.0
87	4.0
88	4.0
89	2.0
90	1.5
91	0.5
92	1.0
93	1.0
94	1.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16522011721671753
2	2.2232618618166528E-4
3	0.012386744658692779
4	0.005939285259424486
5	0.026012163783254837
6	0.002477348931738556
7	0.0
8	0.0
9	3.176088374023789E-5
10-11	0.04792717356401899
12-13	2.2232618618166528E-4
14-15	0.005335828468359966
16-17	1.5880441870118946E-5
18-19	0.0017944899313234414
20-21	2.6996751179202215E-4
22-23	0.0
24-25	5.716959073242821E-4
26-27	0.0029220013041018865
28-29	0.005113502282178301
30-31	0.01648389866118347
32-33	0.011767407425758141
34-35	0.010449330750538268
36-37	0.004192436653711403
38-39	3.176088374023789E-5
40-41	0.0032872514671146223
42-43	0.009448862912720773
44-45	0.007050916190332813
46-47	0.00317608837402379
48-49	0.008257829772461853
50-51	0.00241382716425808
52-53	0.0022867836292971284
54-55	0.004589447700464376
56-57	0.0081307862375009
58-59	0.016102768056300613
60-61	0.008114905795630782
62-63	0.021819727129543432
64-65	0.04197200786272438
66-67	0.062235451688996156
68-69	0.051706718729107294
70-71	0.07025507483340623
72-73	0.034651124160599546
74-75	0.027727251505227684
76-77	0.22767789509189534
78-79	0.0870883432157323
80-81	0.06868291108826445
82-83	0.028092501668240417
84-85	0.0534694477766905
86-87	0.06879407418135527
88-89	0.29124730389798154
90-91	0.2962814039708092
92-93	0.05381881749783312
94-95	0.010036439261915175
96-97	0.015165821985963593
98-99	0.0011116309309083263
100-101	1.2704353496095156E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3148527.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.50457529446869
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.49505935044893	47.493102287130256
2	12.115011350590446	14.660272329102849
3	3.864166598458526	7.0139927672041456
4	1.8063357265748572	4.37166303902549
5	0.9597140108736009	2.9033544316029163
6	0.600641052015774	2.180491905398237
7	0.4122838240349495	1.7461540371809854
8	0.3072129689451156	1.4870232168781614
9	0.21835332332061	1.1890237582484382
>10	1.1485357090395645	12.265102076165139
>50	0.0544721149459827	2.245358964864237
>100	0.016582998591713076	1.6328235936968982
>500	0.0013153001293647543	0.5680540009465932
>1k	3.156720304468596E-4	0.24358359255570375
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.17608837402379E-5
2	0.0	0.0	0.0	3.17608837402379E-5	3.17608837402379E-5
3	0.0	0.0	0.0	3.17608837402379E-5	3.17608837402379E-5
4	0.0	0.0	0.0	3.17608837402379E-5	3.17608837402379E-5
5	0.0	0.0	0.0	3.17608837402379E-5	3.17608837402379E-5
6	0.0	0.0	0.0	3.17608837402379E-5	6.35217674804758E-5
7	0.0	0.0	0.0	3.17608837402379E-5	6.35217674804758E-5
8	0.0	0.0	0.0	3.17608837402379E-5	6.35217674804758E-5
9	0.0	0.0	0.0	3.17608837402379E-5	6.35217674804758E-5
10-11	0.0	0.0	0.0	3.17608837402379E-5	6.35217674804758E-5
12-13	0.0	0.0	0.0	3.17608837402379E-5	2.064457443115463E-4
14-15	0.0	0.0	0.0	3.17608837402379E-5	2.6996751179202215E-4
16-17	0.0	0.0	0.0	4.764132561035685E-5	2.8584795366214105E-4
18-19	0.0	0.0	0.0	6.35217674804758E-5	3.4936972114261685E-4
20-21	0.0	0.0	0.0	7.940220935059473E-5	3.4936972114261685E-4
22-23	0.0	0.0	0.0	1.1116309309083263E-4	3.652501630127358E-4
24-25	0.0	0.0	0.0	2.6996751179202215E-4	4.1289148862309264E-4
26-27	0.0	0.0	0.0	3.811306048828548E-4	4.2877193049321155E-4
28-29	0.0	0.0	0.0	0.0013021962333497538	4.446523723633305E-4
30-31	0.0	0.0	0.0	0.006177491887476271	4.605328142334495E-4
32-33	0.0	1.588044187011895E-5	0.0	0.020628693989284512	4.7641325610356844E-4
34-35	0.0	3.17608837402379E-5	0.0	0.0392246914191938	4.7641325610356844E-4
36-37	0.0	3.17608837402379E-5	0.0	0.06909580257688755	4.7641325610356844E-4
38-39	0.0	3.17608837402379E-5	0.0	0.11918271623524271	4.7641325610356844E-4
40-41	0.0	3.17608837402379E-5	0.0	0.1795125148998246	4.7641325610356844E-4
42-43	0.0	3.17608837402379E-5	0.0	0.24403475021811788	4.7641325610356844E-4
44-45	0.0	3.17608837402379E-5	0.0	0.32118193682315577	5.081741398438064E-4
46-47	3.17608837402379E-5	3.17608837402379E-5	0.0	0.41530531578735075	5.716959073242821E-4
48-49	3.17608837402379E-5	3.17608837402379E-5	0.0	0.5178612093845789	6.0345679106452E-4
50-51	3.17608837402379E-5	3.17608837402379E-5	0.0	0.6292625091034634	6.0345679106452E-4
52-53	3.17608837402379E-5	3.17608837402379E-5	0.0	0.7423947769861907	6.0345679106452E-4
54-55	3.17608837402379E-5	3.17608837402379E-5	0.0	0.8704546602268299	6.0345679106452E-4
56-57	3.17608837402379E-5	3.17608837402379E-5	0.0	1.0195084876197664	6.19337232934639E-4
58-59	3.17608837402379E-5	3.17608837402379E-5	0.0	1.1822988972303556	6.35217674804758E-4
60-61	3.17608837402379E-5	3.17608837402379E-5	0.0	1.3610332704785444	6.35217674804758E-4
62-63	3.17608837402379E-5	3.17608837402379E-5	0.0	1.546167461800391	6.35217674804758E-4
64-65	3.17608837402379E-5	3.17608837402379E-5	0.0	1.744545941641917	6.35217674804758E-4
66-67	3.17608837402379E-5	3.17608837402379E-5	0.0	1.9788618614355222	6.35217674804758E-4
68-69	3.17608837402379E-5	3.17608837402379E-5	0.0	2.2355374433822544	6.35217674804758E-4
70-71	3.17608837402379E-5	3.17608837402379E-5	0.0	2.5085540000133397	6.35217674804758E-4
72-73	3.17608837402379E-5	3.17608837402379E-5	0.0	2.7958788347694017	6.510981166748769E-4
74-75	3.17608837402379E-5	3.17608837402379E-5	0.0	3.096177990533351	6.669785585449958E-4
76-77	3.17608837402379E-5	3.17608837402379E-5	0.0	3.428984410805434	6.669785585449958E-4
78-79	3.17608837402379E-5	3.17608837402379E-5	0.0	3.805112676499201	6.828590004151147E-4
80-81	3.17608837402379E-5	3.17608837402379E-5	0.0	4.2022031254615255	7.305003260254716E-4
82-83	3.17608837402379E-5	3.17608837402379E-5	0.0	4.617444919481396	7.781416516358284E-4
84-85	3.17608837402379E-5	3.17608837402379E-5	0.0	5.057412561493041	7.940220935059474E-4
86-87	3.17608837402379E-5	3.17608837402379E-5	0.0	5.523884660985916	8.416634191163043E-4
88-89	3.17608837402379E-5	3.17608837402379E-5	0.0	6.0323764096671235	8.89304744726661E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2245	0.0	44.041477	1
GTATCAA	4140	0.0	41.56455	1
TCAACGC	4880	0.0	34.23481	4
ATCAACG	5045	0.0	33.02054	3
CAACGCA	5080	0.0	33.008728	5
TATCAAC	5530	0.0	30.639471	2
AACGCAG	5550	0.0	30.378298	6
GTACATG	8250	0.0	25.524937	1
ACGCAGA	6660	0.0	24.45952	7
TACATGG	8640	0.0	23.620708	2
CGCAGAG	6970	0.0	23.030956	8
ACATGGG	8785	0.0	22.420462	3
GTATATA	905	0.0	20.48476	1
TATACCG	280	1.5770638E-9	20.358278	5
CATGGGG	7465	0.0	19.963879	4
TATACTG	1355	0.0	19.632092	5
GCAGAGT	8130	0.0	18.57653	9
AGAGTAC	6870	0.0	17.766605	10-11
CTATACT	1230	0.0	17.749977	4
CCTATAC	855	0.0	17.208134	3
>>END_MODULE