FastQCFastQC Report
Mon 15 Aug 2016
SRR2921693_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921693_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3618057
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25700.048.199551
GTATCAA50400.042.658281
TCAACGC57700.036.1142354
CAACGCA59750.034.8727535
ATCAACG59950.034.836073
TATCAAC63200.033.2699622
AACGCAG63900.032.7370226
GTACATG90050.027.35391
ACGCAGA73750.027.2754127
CGCAGAG76200.026.0907528
TACATGG93100.025.6438942
ACATGGG96750.023.7443393
GCAGAGT90000.021.1942029
CATGGGG81700.021.1479874
AGAGTAC76200.018.21408810-11
CAGAGTA83550.017.37846810-11
GTATAGC13600.017.099871
ATGGGGG50150.016.9411075
GTGTAGC24400.016.9225221
GTATAAT11800.016.4906391
Produced by FastQC (version 0.11.5)