##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921693_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3618057
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.481568974728702	31.0	31.0	34.0	26.0	34.0
2	30.54898361192209	33.0	31.0	34.0	26.0	34.0
3	30.610938136132184	33.0	31.0	34.0	26.0	34.0
4	34.06656694463354	37.0	35.0	37.0	30.0	37.0
5	34.068091243449174	37.0	35.0	37.0	30.0	37.0
6	34.1126803696017	37.0	35.0	37.0	30.0	37.0
7	34.03046303582282	37.0	35.0	37.0	30.0	37.0
8	34.00232113534972	37.0	35.0	37.0	30.0	37.0
9	35.57308301113001	39.0	37.0	39.0	30.0	39.0
10-11	35.4750801327895	39.0	37.0	39.0	30.0	39.0
12-13	35.37268166311365	39.0	35.0	39.0	30.0	39.0
14-15	36.52346715377895	40.0	37.0	41.0	29.0	41.0
16-17	36.469296227229144	40.0	37.0	41.0	29.0	41.0
18-19	36.4403222503128	40.0	37.0	41.0	29.0	41.0
20-21	36.37052304593322	40.0	37.0	41.0	28.0	41.0
22-23	36.27385942233636	40.0	37.0	41.0	28.0	41.0
24-25	36.173226817598504	40.0	36.0	41.0	27.0	41.0
26-27	36.03154110065154	40.0	36.0	41.0	27.0	41.0
28-29	35.89869742240103	40.0	36.0	41.0	27.0	41.0
30-31	35.7548454875089	40.0	36.0	41.0	27.0	41.0
32-33	35.582844742357565	40.0	36.0	41.0	26.0	41.0
34-35	35.41578877834152	39.0	35.0	41.0	25.0	41.0
36-37	35.385096337619885	39.0	35.0	41.0	25.0	41.0
38-39	35.562240865746446	40.0	35.5	41.0	25.5	41.0
40-41	35.62882080077787	40.0	36.0	41.0	26.0	41.0
42-43	35.47390242884509	40.0	35.0	41.0	25.0	41.0
44-45	35.36094152192738	40.0	35.0	41.0	24.5	41.0
46-47	35.21390085894169	40.0	35.0	41.0	24.0	41.0
48-49	35.06152045144673	40.0	35.0	41.0	23.0	41.0
50-51	33.37703897423396	38.0	32.5	39.5	19.5	40.0
52-53	33.49914843795993	37.5	33.5	39.5	21.0	40.0
54-55	34.42331892504734	39.0	34.5	40.5	22.0	41.0
56-57	34.460335616603054	39.0	34.5	41.0	22.0	41.0
58-59	34.288184929093156	39.0	34.0	41.0	21.0	41.0
60-61	34.04869478286274	38.0	34.0	41.0	20.0	41.0
62-63	33.770085158967916	38.0	34.0	40.0	18.5	41.0
64-65	33.496734573280634	37.0	34.0	40.0	18.0	41.0
66-67	33.166944301872526	37.0	33.5	40.0	16.0	41.0
68-69	32.81825797658799	36.0	33.0	39.0	13.0	41.0
70-71	32.41792818631658	35.5	33.0	39.0	9.5	41.0
72-73	32.05877602259998	35.0	33.0	38.5	7.5	40.0
74-75	31.658562731322363	35.0	32.0	37.0	4.5	39.5
76-77	31.277972127028402	35.0	32.0	37.0	2.0	39.0
78-79	30.928442946034295	35.0	32.0	36.0	2.0	39.0
80-81	30.603536511447995	35.0	32.0	36.0	2.0	37.5
82-83	30.289084997831708	35.0	31.0	35.5	2.0	37.0
84-85	30.022519960299135	35.0	31.0	35.0	2.0	37.0
86-87	29.785593621106578	35.0	31.0	35.0	2.0	36.0
88-89	29.563420504430972	35.0	31.0	35.0	2.0	36.0
90-91	29.34555232269696	35.0	31.0	35.0	2.0	36.0
92-93	29.1639558193804	35.0	31.0	35.0	2.0	35.0
94-95	28.942579815630324	34.0	30.5	35.0	2.0	35.0
96-97	28.71766420484807	34.0	30.0	35.0	2.0	35.0
98-99	28.46722273861357	34.0	30.0	35.0	2.0	35.0
100-101	27.412493639541886	33.5	27.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	115184.0
3	20140.0
4	13887.0
5	10583.0
6	9364.0
7	9853.0
8	10415.0
9	11111.0
10	12509.0
11	13195.0
12	13161.0
13	13555.0
14	14556.0
15	15675.0
16	15267.0
17	15958.0
18	15295.0
19	14280.0
20	14519.0
21	15194.0
22	16436.0
23	19098.0
24	22330.0
25	26152.0
26	31102.0
27	37415.0
28	43740.0
29	52036.0
30	61935.0
31	75740.0
32	94741.0
33	122903.0
34	170052.0
35	246402.0
36	409065.0
37	757748.0
38	885160.0
39	172277.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.604251950082535	18.257092045112326	13.180641509193947	24.95801449561119
2	15.549735931806403	19.776624916951423	39.90746134403436	24.766177807207814
3	18.756394395087113	24.088424440411774	29.72174303154416	27.43343813295695
4	13.098233501325005	16.101035083531627	37.42489538666896	33.37583602847441
5	14.070727291534485	37.23382953031197	34.76034908400574	13.935094094147804
6	32.812973800139375	35.74633654449758	17.680531587689497	13.760158067673553
7	28.415721379612318	31.8532804658121	21.59008358036663	18.14091457420895
8	26.051037623474443	35.04690736483085	20.41488352703798	18.487171484656727
9	26.5888941253215	15.80843686037014	19.72563550098609	37.87703351332227
10-11	25.225581217600237	25.356165048121	28.47147673477951	20.946776999499253
12-13	25.901528491389687	22.915552505978628	27.936300948273136	23.246618054358557
14-15	23.10806345695227	24.417765181626518	25.370614868834895	27.10355649258632
16-17	23.334429056703822	27.386799288821162	26.115875372847754	23.162896281627262
18-19	23.29447757709913	26.899658387595533	27.225155565150366	22.580708470154974
20-21	23.93648195051936	26.130763706088516	27.089162902845022	22.843591440547097
22-23	23.684901028356176	26.03541363044043	26.70359768443597	23.576087656767427
24-25	23.531546396997253	26.10331966710568	26.840762342978586	23.524371592918484
26-27	23.382643152065	26.29907374246278	26.74596802005696	23.572315085415262
28-29	23.499796896770626	26.524784191589024	26.57466507316899	23.40075383847136
30-31	23.849866769902313	26.209792726613827	26.472977281846166	23.46736322163769
32-33	23.54002533077335	26.304172722483187	26.38090583774263	23.774896109000828
34-35	23.91437413683327	26.32591323782832	26.38878197318826	23.37093065215015
36-37	23.93510389220208	26.139508555625174	26.429942933016843	23.4954446191559
38-39	23.69536228162637	26.272181138809263	26.462014469401606	23.57044211016276
40-41	23.983493722736277	26.089937375924798	26.456381153490828	23.470187747848094
42-43	23.738293136253162	26.09499178855711	26.323834597703012	23.842880477486712
44-45	23.71407212098915	26.14458897344684	26.444685774846672	23.696653130717337
46-47	23.85002547228976	26.12760405523838	26.179822595437308	23.842547877034544
48-49	23.608163703933897	26.187461189779416	26.321070826956134	23.883304279330556
50-51	23.720965204980534	26.21994154510951	26.386219634135582	23.672873615774375
52-53	23.89756845632026	26.13267593330392	26.298369212540784	23.671386397835033
54-55	23.460872972956132	25.963291127846826	26.616431821187398	23.95940407800964
56-57	23.52090739476716	26.117829984236696	26.468962711327382	23.892299909668765
58-59	23.63399237371009	26.01309124832475	26.506544031485696	23.846372346479473
60-61	23.635210557937608	26.02056065498481	26.630128425840955	23.714100361236632
62-63	23.661253922601926	25.998398347331523	26.603788713126225	23.736559016940323
64-65	23.481970537515377	25.8529501327943	26.54228403426022	24.122795295430098
66-67	23.453629803924176	25.93818562701447	26.47153801458203	24.136646554479324
68-69	23.587728197885944	25.897632700287478	26.534172581927333	23.98046651989924
70-71	23.462490304504332	25.715002133632108	26.527277676503715	24.295229885359852
72-73	23.426236926734322	25.868490784877828	26.483104593879926	24.222167694507927
74-75	23.465474367591575	25.930580122791408	26.468391418171787	24.13555409144523
76-77	23.46310150357721	25.663247998161204	26.598473299354342	24.275177198907244
78-79	23.387522852482885	25.6930944612686	26.65966741215513	24.25971527409338
80-81	23.543750370502924	25.83039747276838	26.503341590123764	24.12251056660493
82-83	23.53753458778726	25.791969954712123	26.587492524273433	24.083002933227185
84-85	23.501611801522664	25.790029266703705	26.58570911788283	24.1226498138908
86-87	23.48737579177914	25.8471536399573	26.513960953463673	24.151509614799892
88-89	23.400211038201014	25.646917410042125	26.632187120907602	24.32068443084925
90-91	23.359531151540875	25.706086771263088	26.690136055316984	24.24424602187906
92-93	23.53408544559306	25.49146390399979	26.8141636681492	24.160286982257947
94-95	23.421141321459686	25.43283997239	26.842567996140254	24.303450710010065
96-97	23.398188538957662	25.30846385161208	26.904218038058108	24.389129571372152
98-99	23.26411783414433	25.20786536153557	26.878711683568135	24.64930512075196
100-101	23.468439944692655	25.148066009186582	26.936550184240065	24.446943861880698
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	56.0
1	45.0
2	56.5
3	84.0
4	85.5
5	93.5
6	154.5
7	328.0
8	501.5
9	600.5
10	588.5
11	549.5
12	614.5
13	713.5
14	809.0
15	929.5
16	1053.0
17	1128.0
18	1262.0
19	1393.0
20	1533.0
21	1682.0
22	1835.0
23	2096.0
24	2419.0
25	2907.0
26	3722.0
27	4922.0
28	6825.5
29	9920.0
30	14525.0
31	21219.5
32	30595.5
33	39669.0
34	47655.5
35	57126.5
36	69021.5
37	81022.0
38	92081.5
39	101938.0
40	110237.5
41	118874.5
42	126993.5
43	134674.5
44	141925.0
45	149443.0
46	154716.0
47	158062.5
48	161525.5
49	162282.0
50	159197.5
51	154982.5
52	150670.5
53	146390.5
54	141842.5
55	132786.0
56	120528.5
57	107178.5
58	95869.5
59	83786.5
60	68120.5
61	55843.0
62	46381.0
63	35292.5
64	25022.5
65	18121.0
66	13482.0
67	10351.0
68	7818.0
69	5799.0
70	4397.0
71	3166.5
72	2214.5
73	1627.5
74	1266.5
75	959.0
76	669.5
77	416.5
78	284.0
79	209.5
80	144.5
81	109.0
82	95.0
83	82.5
84	69.0
85	66.0
86	55.0
87	39.5
88	35.0
89	35.5
90	37.5
91	36.5
92	29.5
93	20.5
94	15.5
95	12.5
96	8.0
97	5.5
98	5.5
99	4.5
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.053288270472245186
2	0.07470860740999935
3	0.07147482751100936
4	0.07186177553311072
5	0.06696964696797203
6	0.010115926863507126
7	0.012741645584909249
8	0.016804599816973586
9	0.006688672953466459
10-11	0.012644908579383907
12-13	0.022042217687559925
14-15	0.03957925483208252
16-17	0.026644135236122593
18-19	0.00996391156911016
20-21	0.019983101427091945
22-23	0.03631783578865673
24-25	0.034092884661573876
26-27	0.020964291054563263
28-29	0.012119764835103481
30-31	0.012257960557282541
32-33	0.008927443652767217
34-35	0.005569287603816081
36-37	7.738960442027308E-4
38-39	0.004049134659846431
40-41	0.001091746205214567
42-43	0.22541104244626328
44-45	0.19500798356687027
46-47	0.20095039962056985
48-49	0.20837150990158532
50-51	0.20006594699862384
52-53	0.20646440893551427
54-55	0.20313389203099894
56-57	0.2060912804856308
58-59	0.2151016415717055
60-61	0.21995231142019045
62-63	0.20484751898601927
64-65	0.20446057096391793
66-67	0.20206978497022018
68-69	0.19702564111068452
70-71	0.1891623045186961
72-73	0.20046671459294313
74-75	0.1994993445376897
76-77	0.19352928933955435
78-79	0.21206133568376617
80-81	0.2238494307856399
82-83	0.22221872126392703
84-85	0.2158893571881261
86-87	0.2361350304873583
88-89	0.2289626725062651
90-91	0.23583099989856438
92-93	0.23096651047786143
94-95	0.23480835155443933
96-97	0.24213272482992942
98-99	0.23518148000432276
100-101	0.24243675541872337
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3618057.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.134826308925405
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.97403290491017	50.491466766757895
2	11.569824573187852	14.60917729705904
3	3.5754181110223993	6.772002042635561
4	1.6111550336507172	4.068799728251556
5	0.8535361561000123	2.6943928481881065
6	0.5233934440203057	1.9826612507671366
7	0.36469073475126257	1.6117280336496744
8	0.2670627279229883	1.3488767152804537
9	0.19705859026451839	1.1197133882128862
>10	1.0034221793511349	11.094285056804114
>50	0.04556492949618772	1.9451864614390297
>100	0.013306367278087752	1.3698652779509364
>500	0.0010520558111987229	0.46450671425092616
>1k	4.821922332762398E-4	0.42733841875272577
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.763914443581182E-5
2	0.0	0.0	0.0	0.0	2.763914443581182E-5
3	0.0	0.0	0.0	0.0	2.763914443581182E-5
4	0.0	0.0	0.0	0.0	1.1055657774324728E-4
5	0.0	0.0	0.0	0.0	1.1055657774324728E-4
6	0.0	0.0	0.0	0.0	1.1055657774324728E-4
7	0.0	0.0	0.0	0.0	1.1055657774324728E-4
8	0.0	0.0	0.0	0.0	1.1055657774324728E-4
9	0.0	0.0	0.0	1.381957221790591E-4	1.1055657774324728E-4
10-11	0.0	0.0	0.0	2.0729358326858864E-4	1.1055657774324728E-4
12-13	0.0	1.381957221790591E-5	0.0	2.2111315548649455E-4	2.2111315548649455E-4
14-15	0.0	2.763914443581182E-5	0.0	2.2111315548649455E-4	3.0403058879393E-4
16-17	0.0	2.763914443581182E-5	0.0	2.2111315548649455E-4	3.5930887766555365E-4
18-19	0.0	2.763914443581182E-5	0.0	2.625718721402123E-4	3.8694802210136547E-4
20-21	0.0	2.763914443581182E-5	0.0	3.3166973322974183E-4	4.145871665371773E-4
22-23	0.0	2.763914443581182E-5	0.0	3.4548930544764774E-4	4.422263109729891E-4
24-25	0.0	2.763914443581182E-5	0.0	4.698654554088009E-4	4.698654554088009E-4
26-27	0.0	2.763914443581182E-5	0.0	7.877156164206368E-4	4.975045998446127E-4
28-29	0.0	2.763914443581182E-5	0.0	0.002584260004748405	4.975045998446127E-4
30-31	0.0	4.145871665371773E-5	0.0	0.010710168468877079	5.251437442804246E-4
32-33	0.0	5.527828887162364E-5	0.0	0.0318126552456194	5.804220331520482E-4
34-35	0.0	5.527828887162364E-5	0.0	0.060723200325478566	6.0806117758786E-4
36-37	0.0	5.527828887162364E-5	0.0	0.10701876725546336	6.218807498057659E-4
38-39	0.0	8.291743330743546E-5	0.0	0.18581796804196288	6.633394664594837E-4
40-41	0.0	1.1055657774324728E-4	0.0	0.2763361660692466	7.047981831132014E-4
42-43	0.0	1.1055657774324728E-4	0.0	0.3711384314840811	7.186177553311073E-4
44-45	0.0	1.1055657774324728E-4	0.0	0.49005585041916144	7.462568997669191E-4
46-47	0.0	1.1055657774324728E-4	0.0	0.6296611689644469	7.738960442027309E-4
48-49	0.0	1.1055657774324728E-4	0.0	0.7756373103021871	8.015351886385427E-4
50-51	0.0	1.1055657774324728E-4	0.0	0.9302783234205542	8.015351886385427E-4
52-53	0.0	1.1055657774324728E-4	0.0	1.0921055140922324	8.706330497280724E-4
54-55	0.0	1.1055657774324728E-4	0.0	1.2777576472675802	8.844526219459782E-4
56-57	0.0	1.1055657774324728E-4	0.0	1.4898604416680001	8.982721941638841E-4
58-59	0.0	1.1055657774324728E-4	0.0	1.7140277226146519	9.25911338599696E-4
60-61	0.0	1.1055657774324728E-4	0.0	1.9493473983411538	9.397309108176018E-4
62-63	0.0	1.1055657774324728E-4	0.0	2.197947682969063	9.673700552534137E-4
64-65	0.0	1.1055657774324728E-4	0.0	2.471768686894651	9.673700552534137E-4
66-67	0.0	1.1055657774324728E-4	0.0	2.783082190247417	9.811896274713196E-4
68-69	0.0	1.1055657774324728E-4	0.0	3.118994532148056	0.0010088287719071313
70-71	0.0	1.1055657774324728E-4	0.0	3.4763410305586673	0.0010502874885608491
72-73	2.763914443581182E-5	1.1055657774324728E-4	0.0	3.847935507925939	0.001064107060778755
74-75	2.763914443581182E-5	1.1055657774324728E-4	0.0	4.243534582235714	0.001091746205214567
76-77	2.763914443581182E-5	1.1055657774324728E-4	0.0	4.684613868714617	0.0011055657774324728
78-79	2.763914443581182E-5	1.1055657774324728E-4	0.0	5.169957245007472	0.0011055657774324728
80-81	2.763914443581182E-5	1.1055657774324728E-4	0.0	5.677619230432246	0.0011332049218682845
82-83	4.145871665371773E-5	1.1055657774324728E-4	0.0	6.208290803599833	0.001188483210739908
84-85	5.527828887162364E-5	1.1055657774324728E-4	0.0	6.763892885048522	0.001188483210739908
86-87	5.527828887162364E-5	1.1055657774324728E-4	0.0	7.358410881862834	0.0012023027829578142
88-89	5.527828887162364E-5	1.1055657774324728E-4	0.0	7.998160338546352	0.0012437614996115317
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2570	0.0	48.19955	1
GTATCAA	5040	0.0	42.65828	1
TCAACGC	5770	0.0	36.114235	4
CAACGCA	5975	0.0	34.872753	5
ATCAACG	5995	0.0	34.83607	3
TATCAAC	6320	0.0	33.269962	2
AACGCAG	6390	0.0	32.737022	6
GTACATG	9005	0.0	27.3539	1
ACGCAGA	7375	0.0	27.275412	7
CGCAGAG	7620	0.0	26.090752	8
TACATGG	9310	0.0	25.643894	2
ACATGGG	9675	0.0	23.744339	3
GCAGAGT	9000	0.0	21.194202	9
CATGGGG	8170	0.0	21.147987	4
AGAGTAC	7620	0.0	18.214088	10-11
CAGAGTA	8355	0.0	17.378468	10-11
GTATAGC	1360	0.0	17.09987	1
ATGGGGG	5015	0.0	16.941107	5
GTGTAGC	2440	0.0	16.922522	1
GTATAAT	1180	0.0	16.490639	1
>>END_MODULE