FastQCFastQC Report
Mon 15 Aug 2016
SRR2921693_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921693_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3618057
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26150.043.9869041
GTATCAA45000.043.7287981
TCAACGC56300.033.971094
CAACGCA57700.033.501775
ATCAACG59900.032.1671073
TATCAAC60400.031.9008242
AACGCAG63550.030.2633156
ACGCAGA75800.024.6826657
GTACATG91200.024.3389451
CGCAGAG79500.023.1755288
TACATGG94350.022.635152
ACATGGG94650.022.3127023
CATGGGG79300.020.347854
GCAGAGT91650.019.0158889
AGAGTAC79050.017.15091310-11
GTATTAG9550.016.922141
ATGGGGG45100.016.5337855
CAGAGTA85250.015.51364510-11
CTATACT15150.015.3495724
GTATACG2801.3309258E-515.2779191
Produced by FastQC (version 0.11.5)