##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921693_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3618057
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.408367253473344	33.0	31.0	34.0	29.0	34.0
2	31.55267205574705	34.0	31.0	34.0	29.0	34.0
3	31.626015565813365	34.0	31.0	34.0	28.0	34.0
4	35.10338228502204	37.0	35.0	37.0	32.0	37.0
5	34.84986195629312	37.0	35.0	37.0	32.0	37.0
6	34.82262523780029	37.0	35.0	37.0	32.0	37.0
7	34.71540967983644	37.0	35.0	37.0	32.0	37.0
8	34.65872676964459	37.0	35.0	37.0	32.0	37.0
9	36.24890099851937	39.0	37.0	39.0	32.0	39.0
10-11	36.147606159880844	39.0	37.0	39.0	32.0	39.0
12-13	36.07217492703957	39.0	37.0	39.0	32.0	39.0
14-15	37.320468140772796	40.0	38.0	41.0	32.0	41.0
16-17	37.287514818036314	40.0	38.0	41.0	31.5	41.0
18-19	37.253435338359786	40.0	38.0	41.0	31.0	41.0
20-21	37.19944904129482	40.0	38.0	41.0	31.0	41.0
22-23	37.11105795182331	40.0	38.0	41.0	31.0	41.0
24-25	37.03141326960852	40.0	37.5	41.0	31.0	41.0
26-27	36.906052336931126	40.0	37.0	41.0	30.0	41.0
28-29	36.791872129156616	40.0	37.0	41.0	30.0	41.0
30-31	36.63799602936051	40.0	37.0	41.0	30.0	41.0
32-33	36.583929164189506	40.0	37.0	41.0	30.0	41.0
34-35	36.71191830311131	40.0	37.0	41.0	30.0	41.0
36-37	36.70895759795934	40.0	37.0	41.0	30.0	41.0
38-39	36.606458107210585	40.0	37.0	41.0	30.0	41.0
40-41	36.52721032310989	40.0	37.0	41.0	30.0	41.0
42-43	36.41010036602519	40.0	37.0	41.0	30.0	41.0
44-45	36.28788822287764	40.0	36.5	41.0	29.0	41.0
46-47	36.14111220469992	40.0	36.0	41.0	28.5	41.0
48-49	36.004597909872615	40.0	36.0	41.0	28.0	41.0
50-51	35.85618731269297	40.0	35.5	41.0	28.0	41.0
52-53	35.687639525856	39.5	35.0	41.0	27.5	41.0
54-55	35.48756404335255	39.0	35.0	41.0	27.0	41.0
56-57	35.274156957726206	39.0	35.0	41.0	26.5	41.0
58-59	35.04672977788907	39.0	35.0	41.0	26.0	41.0
60-61	34.78044099360513	38.0	35.0	40.0	26.0	41.0
62-63	34.48944516352285	38.0	34.0	40.0	26.0	41.0
64-65	34.16144936356724	37.0	34.0	40.0	25.5	41.0
66-67	33.818579419837775	37.0	34.0	39.5	24.5	41.0
68-69	33.463847308099346	36.0	34.0	39.0	23.5	41.0
70-71	33.06676691384354	36.0	33.5	39.0	22.5	40.5
72-73	32.699874407727684	35.0	33.0	38.0	22.0	40.0
74-75	32.2880213053581	35.0	33.0	37.0	21.5	39.0
76-77	29.55601321372217	33.0	29.0	34.5	15.5	36.5
78-79	31.125432103474324	34.5	31.5	36.0	18.5	38.0
80-81	31.292895744870798	35.0	32.0	36.0	19.0	37.0
82-83	31.10084722269439	35.0	32.0	35.5	18.0	37.0
84-85	30.89098306079755	35.0	32.0	35.0	18.0	36.5
86-87	30.667726351464335	35.0	32.0	35.0	14.5	36.0
88-89	30.392176242662842	35.0	32.0	35.0	7.0	36.0
90-91	30.189828131508154	34.5	32.0	35.0	4.5	35.5
92-93	30.02504631629629	34.0	31.0	35.0	2.0	35.0
94-95	29.91882963701235	34.0	31.0	35.0	2.0	35.0
96-97	29.7183242276172	34.0	31.0	35.0	2.0	35.0
98-99	29.51971666007473	34.0	31.0	35.0	2.0	35.0
100-101	28.407846808383617	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65743.0
3	22788.0
4	12410.0
5	8634.0
6	7288.0
7	7307.0
8	7672.0
9	8558.0
10	9356.0
11	10544.0
12	10967.0
13	11861.0
14	12908.0
15	13668.0
16	14709.0
17	15661.0
18	14720.0
19	13751.0
20	13085.0
21	12871.0
22	13518.0
23	15509.0
24	18178.0
25	21663.0
26	26184.0
27	31456.0
28	38016.0
29	45923.0
30	55477.0
31	69213.0
32	87714.0
33	116064.0
34	163875.0
35	245309.0
36	425943.0
37	844613.0
38	973029.0
39	131868.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.41823788719437	19.81635649220801	13.77090988722095	25.99449573337667
2	14.693763384274188	21.118574304625803	38.435738347596995	25.75192396350301
3	17.4769205423137	25.154694631346487	29.480339373138793	27.88804545320102
4	12.766195040350087	17.340200053291525	36.31441557881562	33.579189327542764
5	13.870453707282307	37.58333153635237	34.7431340122051	13.803080744160226
6	32.76453653245947	36.35310564877495	17.37028978537242	13.512068033393145
7	28.251821350520455	32.07202097700506	21.626110368078777	18.050047304395704
8	25.97855699896381	35.54181153033244	20.209714772321167	18.269916698382584
9	26.388176412968733	15.763713994476591	19.627197588981485	38.22091200357319
10-11	25.18813152663651	25.64810614424493	28.39822010783714	20.765542221281414
12-13	25.794414712271085	23.07404753014131	27.981150572920537	23.15038718466706
14-15	22.983214170314987	24.628490275916263	25.296742035997394	27.091553517771356
16-17	23.28412736449426	27.603420841628534	26.0546475635956	23.05780423028161
18-19	23.253197516254474	27.109219214409276	27.1809991339146	22.456584135421647
20-21	23.82689241070571	26.273707345986463	27.091993326240193	22.80740691706763
22-23	23.585338760555736	26.134607608448402	26.72256130845921	23.557492322536653
24-25	23.43452359235784	26.316349533282697	26.783564342643356	23.465562531716103
26-27	23.278712675549865	26.520766505152704	26.73833425443417	23.46218656486326
28-29	23.437235038352107	26.682248628574058	26.54534443511265	23.33517189796118
30-31	23.763592975433646	26.385400202767283	26.421862376717286	23.429144445081786
32-33	23.519884078818993	26.42112012268804	26.312209055070717	23.74678674342225
34-35	23.80972322712569	26.48870718852081	26.390163302284407	23.31140628206909
36-37	23.83374190176281	26.307983295360287	26.427306099061976	23.43096870381493
38-39	23.64177789349366	26.449182530844595	26.388680443674602	23.520359131987142
40-41	23.897029913144955	26.245337623822717	26.403245838878064	23.454386624154264
42-43	23.737919588958512	26.208842710235707	26.241556531057952	23.81168116974783
44-45	23.647055929354025	26.274568012617568	26.358665757830558	23.719710300197853
46-47	23.75547344580674	26.25131221734465	26.17132154866799	23.82189278818062
48-49	23.505121184127674	26.35560593722341	26.316009152731084	23.82326372591783
50-51	23.677249627828324	26.349063586767546	26.282769211548846	23.690917573855277
52-53	23.831580995620065	26.189114274235887	26.21790108365823	23.761403646485817
54-55	23.431519773969136	26.111034179097175	26.49105990110873	23.966386145824956
56-57	23.490627904485823	26.226720060257826	26.341845672251342	23.940806363005013
58-59	23.542386135989133	26.15022256843672	26.416439463403158	23.890951832170988
60-61	23.553792793868553	26.182055543024944	26.487102134773323	23.777049528333176
62-63	23.555404308208796	26.169710396654764	26.444429823458037	23.8304554716784
64-65	23.36131672541778	26.007632990467194	26.391453019339068	24.23959726477596
66-67	23.34455304639961	26.192526625731443	26.287038258474865	24.175882069394078
68-69	23.4317465619708	26.083616586603604	26.333332042885633	24.15130480853996
70-71	23.349001158838284	25.892927767590695	26.262400814229096	24.495670259341924
72-73	23.311528770397107	26.058436449852756	26.192141980011357	24.43789279973878
74-75	23.293653777676532	26.154971988950397	26.142323888681236	24.409050344691835
76-77	23.254452760921605	25.79541115315902	26.358425007177903	24.59171107874147
78-79	23.194839668396387	25.85470032273511	26.391029253073068	24.559430755795443
80-81	23.330221022040256	25.940220391465335	26.186186094827406	24.543372491667007
82-83	23.259645199959305	25.9558114486157	26.275484250523068	24.50905910090193
84-85	23.172363903440466	25.93418441614753	26.226848984142713	24.66660269626929
86-87	23.083281095356053	25.965254796179238	26.19221169442021	24.7592524140445
88-89	23.004316209352854	25.811120416877387	26.251486585767942	24.933076788001816
90-91	22.91073833531	25.800647844678053	26.31331582629025	24.975297993721693
92-93	23.008701257382917	25.62567379057832	26.40114219298897	24.964482759049794
94-95	22.83458124436367	25.5719878237975	26.417370092288984	25.17606083954985
96-97	22.71618968059226	25.448335028093116	26.50078154295335	25.334693748361275
98-99	22.55688422575997	25.37271334374508	26.412050098555056	25.658352331939895
100-101	22.491377263615135	25.406634677067846	26.451603363247226	25.65038469606979
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	38.5
2	53.0
3	84.0
4	81.0
5	81.5
6	124.0
7	263.0
8	416.5
9	453.0
10	399.0
11	354.0
12	351.5
13	367.5
14	408.5
15	457.0
16	517.5
17	581.5
18	673.0
19	764.0
20	878.0
21	1066.5
22	1276.5
23	1595.5
24	1996.5
25	2540.0
26	3455.5
27	4871.5
28	7019.5
29	10342.0
30	15349.5
31	22031.5
32	31499.0
33	40466.0
34	48297.0
35	57741.5
36	69140.5
37	81110.5
38	91778.5
39	101482.5
40	110036.0
41	118274.5
42	126536.5
43	134337.5
44	141501.0
45	149243.5
46	154834.5
47	158368.0
48	162350.5
49	163429.5
50	160269.0
51	156426.0
52	152544.0
53	148645.0
54	144288.0
55	134538.5
56	121495.0
57	108219.5
58	96120.5
59	83316.0
60	67930.5
61	55466.5
62	45650.5
63	34402.0
64	24095.5
65	17324.5
66	12841.5
67	9701.5
68	7217.0
69	5389.5
70	3915.0
71	2734.5
72	1927.5
73	1391.5
74	1036.0
75	698.0
76	454.0
77	277.5
78	154.5
79	91.5
80	53.0
81	35.0
82	22.0
83	14.0
84	6.5
85	4.5
86	6.0
87	3.0
88	2.0
89	3.0
90	2.5
91	1.5
92	0.5
93	1.0
94	1.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16445290939308033
2	1.381957221790591E-4
3	0.012327058418372071
4	0.006108250920314412
5	0.02429480795907859
6	0.0019071009660710154
7	0.0
8	0.0
9	2.7639144435811816E-5
10-11	0.04893510522360482
12-13	4.4222631097298905E-4
14-15	0.005085602576189375
16-17	0.0
18-19	0.0014786942273159322
20-21	1.7965443883277682E-4
22-23	0.0
24-25	5.804220331520482E-4
26-27	0.0028606514491065233
28-29	0.005486370170508646
30-31	0.016846058533627306
32-33	0.01178809510187374
34-35	0.010489055313390585
36-37	0.0038694802210136547
38-39	0.0
40-41	0.0036621866377450655
42-43	0.008844526219459782
44-45	0.006716312097902272
46-47	0.0037312844988345952
48-49	0.009369669963740206
50-51	0.0028468318768886175
52-53	0.0022249511270828514
54-55	0.003980036798756902
56-57	0.0074487494254512855
58-59	0.014317076817750522
60-61	0.006882146964517142
62-63	0.02017657543814263
64-65	0.0383631324769068
66-67	0.05804220331520482
68-69	0.04811975046274838
70-71	0.06542185487956657
72-73	0.03188175310670893
74-75	0.02523453886989619
76-77	0.2217073970918645
78-79	0.08341493790728007
80-81	0.06381878450228949
82-83	0.025455652025382684
84-85	0.04977809912889708
86-87	0.06612665306267979
88-89	0.28682522138263716
90-91	0.29177262823664746
92-93	0.05128443250064883
94-95	0.009521685258137172
96-97	0.014745483556505604
98-99	7.877156164206369E-4
100-101	1.6583486661487091E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3618057.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.615845086126185
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.52148054066663	49.7930470655378
2	11.790819095557184	14.765842038518942
3	3.698499790166232	6.9475406973635705
4	1.6031543498808107	4.0153145768514475
5	0.9037721350932048	2.829522800207681
6	0.5564081585053868	2.090398023457803
7	0.37744830574784327	1.6543971248509421
8	0.26583533279472604	1.3316403213354695
9	0.19246956721367628	1.0846480144000776
>10	1.0274610797832804	11.218029602487894
>50	0.047712708331000594	2.0188047348633305
>100	0.01338868387382244	1.3779860374172404
>500	0.001063030220110152	0.4641350094317576
>1k	4.8722216608295315E-4	0.40869395327611396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.291743330743546E-5
2	0.0	0.0	0.0	5.527828887162364E-5	8.291743330743546E-5
3	0.0	0.0	0.0	5.527828887162364E-5	8.291743330743546E-5
4	0.0	0.0	0.0	5.527828887162364E-5	8.291743330743546E-5
5	0.0	0.0	0.0	5.527828887162364E-5	8.291743330743546E-5
6	0.0	0.0	0.0	5.527828887162364E-5	1.6583486661487091E-4
7	0.0	0.0	0.0	5.527828887162364E-5	1.6583486661487091E-4
8	0.0	0.0	0.0	5.527828887162364E-5	1.6583486661487091E-4
9	0.0	0.0	0.0	1.1055657774324728E-4	1.9347401105068273E-4
10-11	0.0	0.0	0.0	1.381957221790591E-4	1.9347401105068273E-4
12-13	2.763914443581182E-5	0.0	0.0	1.381957221790591E-4	1.9347401105068273E-4
14-15	2.763914443581182E-5	0.0	0.0	1.381957221790591E-4	2.2111315548649455E-4
16-17	2.763914443581182E-5	0.0	0.0	1.6583486661487091E-4	2.2111315548649455E-4
18-19	2.763914443581182E-5	2.763914443581182E-5	0.0	1.9347401105068273E-4	3.0403058879393E-4
20-21	2.763914443581182E-5	2.763914443581182E-5	0.0	1.9347401105068273E-4	3.0403058879393E-4
22-23	2.763914443581182E-5	2.763914443581182E-5	0.0	2.0729358326858864E-4	3.3166973322974183E-4
24-25	2.763914443581182E-5	2.763914443581182E-5	0.0	3.3166973322974183E-4	3.5930887766555365E-4
26-27	2.763914443581182E-5	2.763914443581182E-5	0.0	6.495198942415778E-4	3.8694802210136547E-4
28-29	2.763914443581182E-5	2.763914443581182E-5	0.0	0.002390785993697722	3.8694802210136547E-4
30-31	2.763914443581182E-5	4.145871665371773E-5	0.0	0.010544333602262208	3.8694802210136547E-4
32-33	2.763914443581182E-5	5.527828887162364E-5	0.0	0.032102866262195426	4.422263109729891E-4
34-35	2.763914443581182E-5	5.527828887162364E-5	0.0	0.06102723091427249	4.975045998446127E-4
36-37	2.763914443581182E-5	5.527828887162364E-5	0.0	0.1076130088608333	4.975045998446127E-4
38-39	2.763914443581182E-5	6.909786108952955E-5	0.0	0.1866471423750372	5.389633164983305E-4
40-41	2.763914443581182E-5	8.291743330743546E-5	0.0	0.2776490254299476	5.527828887162364E-4
42-43	2.763914443581182E-5	8.291743330743546E-5	0.0	0.3729764345890626	5.666024609341422E-4
44-45	2.763914443581182E-5	8.291743330743546E-5	0.0	0.49219788411293686	5.942416053699542E-4
46-47	2.763914443581182E-5	8.291743330743546E-5	0.0	0.6326185574190788	6.0806117758786E-4
48-49	2.763914443581182E-5	8.291743330743546E-5	0.0	0.7782077507347176	6.0806117758786E-4
50-51	2.763914443581182E-5	8.291743330743546E-5	0.0	0.9326276506975981	6.0806117758786E-4
52-53	2.763914443581182E-5	8.291743330743546E-5	0.0	1.0942751869304437	6.909786108952955E-4
54-55	2.763914443581182E-5	8.291743330743546E-5	0.0	1.279982598394663	7.186177553311073E-4
56-57	2.763914443581182E-5	8.291743330743546E-5	0.0	1.4918780992118146	7.186177553311073E-4
58-59	2.763914443581182E-5	1.1055657774324728E-4	0.0	1.7170818480748091	7.186177553311073E-4
60-61	2.763914443581182E-5	1.1055657774324728E-4	0.0	1.9534656308620897	7.186177553311073E-4
62-63	2.763914443581182E-5	1.1055657774324728E-4	0.0	2.201499313029065	7.738960442027309E-4
64-65	2.763914443581182E-5	1.1055657774324728E-4	0.0	2.4758454606989333	7.738960442027309E-4
66-67	2.763914443581182E-5	1.381957221790591E-4	0.0	2.7877255665126337	7.738960442027309E-4
68-69	2.763914443581182E-5	1.381957221790591E-4	0.0	3.12345825397444	7.738960442027309E-4
70-71	4.145871665371773E-5	1.381957221790591E-4	0.0	3.4810120459683196	7.738960442027309E-4
72-73	5.527828887162364E-5	1.381957221790591E-4	0.0	3.8543892481517013	8.982721941638841E-4
74-75	5.527828887162364E-5	1.381957221790591E-4	0.0	4.25279369562171	9.950091996892255E-4
76-77	5.527828887162364E-5	1.381957221790591E-4	0.0	4.695406954616802	9.950091996892255E-4
78-79	5.527828887162364E-5	1.381957221790591E-4	0.0	5.182795627597907	9.950091996892255E-4
80-81	5.527828887162364E-5	1.381957221790591E-4	0.0	5.69257200757202	0.0010226483441250372
82-83	5.527828887162364E-5	1.381957221790591E-4	0.0	6.226891947805134	0.0011055657774324728
84-85	8.291743330743546E-5	1.381957221790591E-4	0.0	6.787206503380129	0.0011055657774324728
86-87	8.291743330743546E-5	1.381957221790591E-4	0.0	7.386312598170786	0.0011055657774324728
88-89	8.291743330743546E-5	1.381957221790591E-4	0.0	8.031202935719365	0.0011055657774324728
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2615	0.0	43.986904	1
GTATCAA	4500	0.0	43.728798	1
TCAACGC	5630	0.0	33.97109	4
CAACGCA	5770	0.0	33.50177	5
ATCAACG	5990	0.0	32.167107	3
TATCAAC	6040	0.0	31.900824	2
AACGCAG	6355	0.0	30.263315	6
ACGCAGA	7580	0.0	24.682665	7
GTACATG	9120	0.0	24.338945	1
CGCAGAG	7950	0.0	23.175528	8
TACATGG	9435	0.0	22.63515	2
ACATGGG	9465	0.0	22.312702	3
CATGGGG	7930	0.0	20.34785	4
GCAGAGT	9165	0.0	19.015888	9
AGAGTAC	7905	0.0	17.150913	10-11
GTATTAG	955	0.0	16.92214	1
ATGGGGG	4510	0.0	16.533785	5
CAGAGTA	8525	0.0	15.513645	10-11
CTATACT	1515	0.0	15.349572	4
GTATACG	280	1.3309258E-5	15.277919	1
>>END_MODULE