FastQCFastQC Report
Mon 15 Aug 2016
SRR2921692_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921692_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3567625
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49000.044.167441
GGTATCA26600.043.5353131
TCAACGC57800.036.2158784
ATCAACG60300.034.7916453
CAACGCA60100.034.6704985
TATCAAC62150.033.8333322
AACGCAG65850.031.8396686
GTACATG96800.028.0449141
ACGCAGA74500.027.4478457
TACATGG98550.026.7793732
CGCAGAG77550.025.390188
ACATGGG103550.024.2479823
CATGGGG91000.022.0119934
GCAGAGT89800.020.3418259
AGAGTAC76700.018.55814210-11
GTATAGA10550.018.4444141
ATGGGGG53300.017.8994165
GTATAAC8550.017.763041
GTATTAG9550.017.3939461
TAAGGTG17100.017.2093945
Produced by FastQC (version 0.11.5)