##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921692_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3567625
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.32284418906135	31.0	31.0	34.0	26.0	34.0
2	30.383060439367927	31.0	31.0	34.0	26.0	34.0
3	30.443326302512176	33.0	31.0	34.0	26.0	34.0
4	33.893410321992924	37.0	35.0	37.0	30.0	37.0
5	33.89245029956904	37.0	35.0	37.0	30.0	37.0
6	33.93348852527942	37.0	35.0	37.0	30.0	37.0
7	33.84628205038366	37.0	35.0	37.0	29.0	37.0
8	33.810693668757224	37.0	35.0	37.0	29.0	37.0
9	35.36544984408395	39.0	37.0	39.0	29.0	39.0
10-11	35.26021863284398	39.0	35.0	39.0	29.0	39.0
12-13	35.15200798850776	39.0	35.0	39.0	28.0	39.0
14-15	36.28829991941418	40.0	37.0	41.0	27.5	41.0
16-17	36.22590140499632	40.0	36.5	41.0	27.0	41.0
18-19	36.19254812375179	40.0	36.5	41.0	27.0	41.0
20-21	36.11528818191374	40.0	36.5	41.0	27.0	41.0
22-23	36.0177416348411	40.0	36.0	41.0	27.0	41.0
24-25	35.906359412774606	40.0	36.0	41.0	27.0	41.0
26-27	35.762602011141865	40.0	36.0	41.0	27.0	41.0
28-29	35.62148544199573	40.0	36.0	41.0	26.0	41.0
30-31	35.4785079709891	40.0	36.0	41.0	25.0	41.0
32-33	35.3065954241267	39.5	35.0	41.0	25.0	41.0
34-35	35.13543772117305	39.0	35.0	41.0	24.5	41.0
36-37	35.10782355208297	39.0	35.0	41.0	24.5	41.0
38-39	35.2935859290144	39.5	35.0	41.0	24.5	41.0
40-41	35.35617420552889	40.0	35.0	41.0	24.5	41.0
42-43	35.2026845590554	40.0	35.0	41.0	24.0	41.0
44-45	35.08606243649487	40.0	35.0	41.0	23.5	41.0
46-47	34.93146252759189	40.0	35.0	41.0	22.5	41.0
48-49	34.769822921411304	39.0	35.0	41.0	22.0	41.0
50-51	33.07886717353982	37.0	32.5	39.5	18.0	40.0
52-53	33.19909253354823	37.5	33.0	39.5	16.5	40.0
54-55	34.11393896499772	39.0	34.0	40.5	19.0	41.0
56-57	34.135386006096496	39.0	34.0	41.0	18.5	41.0
58-59	33.946459199046984	38.0	34.0	41.0	18.0	41.0
60-61	33.699681160435865	38.0	34.0	40.0	16.0	41.0
62-63	33.411552223117624	37.0	34.0	40.0	13.0	41.0
64-65	33.12015276269227	37.0	33.0	40.0	12.0	41.0
66-67	32.78131740303423	36.0	33.0	39.5	8.0	41.0
68-69	32.42549497214533	36.0	33.0	39.0	7.0	41.0
70-71	32.02244756665849	35.0	32.0	39.0	4.0	40.5
72-73	31.66632872008689	35.0	32.0	37.5	2.0	40.0
74-75	31.272429137030937	35.0	32.0	37.0	2.0	39.0
76-77	30.905970919028768	35.0	32.0	36.5	2.0	39.0
78-79	30.573219438702218	35.0	31.0	36.0	2.0	38.5
80-81	30.26424414000911	35.0	31.0	36.0	2.0	37.0
82-83	29.96684292771802	35.0	31.0	35.0	2.0	37.0
84-85	29.71664048211345	35.0	31.0	35.0	2.0	36.5
86-87	29.49194856522196	35.0	31.0	35.0	2.0	36.0
88-89	29.281690900809362	35.0	31.0	35.0	2.0	36.0
90-91	29.06977078588697	34.5	30.0	35.0	2.0	35.5
92-93	28.89149826565292	34.0	30.0	35.0	2.0	35.0
94-95	28.67506268175607	34.0	30.0	35.0	2.0	35.0
96-97	28.45560519953751	34.0	30.0	35.0	2.0	35.0
98-99	28.206767807715217	34.0	30.0	35.0	2.0	35.0
100-101	27.14925405556918	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	127525.0
3	22149.0
4	14820.0
5	11109.0
6	10284.0
7	10455.0
8	11069.0
9	11680.0
10	12583.0
11	13285.0
12	13475.0
13	13533.0
14	14291.0
15	14931.0
16	15427.0
17	15392.0
18	15048.0
19	14325.0
20	14477.0
21	15295.0
22	16499.0
23	19275.0
24	22454.0
25	26556.0
26	31511.0
27	37738.0
28	44536.0
29	52807.0
30	62588.0
31	76476.0
32	95591.0
33	123824.0
34	173335.0
35	250790.0
36	418451.0
37	762770.0
38	819748.0
39	141508.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.81972651654253	17.9474465500738	13.095845415126147	25.136981518257517
2	15.763381530171294	19.488315925153476	39.60450448049662	25.143798064178608
3	19.032598917773264	23.885116002352838	29.158411772939395	27.923873306934503
4	13.370402669710305	15.826594495251234	36.55095846291378	34.25204437212467
5	14.554527095254125	36.887355548075845	34.210030292830695	14.348087063839335
6	33.42860723036931	35.09689091820919	17.32977616707954	14.144725684341955
7	29.0430944195132	31.43901675962619	21.180726093479826	18.337162727380786
8	26.595744382609155	34.61768885532196	20.05697780461637	18.729588957452517
9	26.93551642363389	15.726519445425547	19.30389469140163	38.03406943953894
10-11	25.707179988694033	25.018743989413377	27.90365736618109	21.370418655711497
12-13	26.286518125802978	22.5081953539685	27.603746487580278	23.601540032648245
14-15	23.567872930128843	24.022436993924003	24.706659592772514	27.70303048317464
16-17	23.84771500023201	27.021078513217546	25.544998004425175	23.586208482125272
18-19	23.86513707948196	26.51048977121509	26.67263206790017	22.951741081402776
20-21	24.363367169240608	25.781444607690524	26.51941624473996	23.33577197832891
22-23	24.278756876367755	25.523439551545223	26.23731858074938	23.960484991337637
24-25	24.07276296951381	25.706580469616156	26.213828390630106	24.00682817023992
26-27	23.901054806274477	25.86782477097876	26.21158617367892	24.019534249067846
28-29	24.007263987001522	26.003290547986413	26.07670974355984	23.912735721452226
30-31	24.284592361911514	25.790262962880423	25.87157290373203	24.053571771476033
32-33	24.04492444963975	25.877727623448678	25.832035090743826	24.24531283616774
34-35	24.380954917125212	25.872196511213957	25.900269942227318	23.846578629433512
36-37	24.400655005615814	25.6996504375627	25.94663699968859	23.953057557132897
38-39	24.239904863283016	25.821784037690286	25.908875899702803	24.029435199323895
40-41	24.396912359461748	25.640416882207358	25.97436888914988	23.988301869181015
42-43	24.23881204179649	25.650462686250584	25.82010799032613	24.290617281626798
44-45	24.131456308879944	25.707752977155923	25.996600505149658	24.164190208814475
46-47	24.36779852916765	25.660485628024855	25.771656128688143	24.200059714119355
48-49	24.10708680350102	25.731377385098764	25.822218895174544	24.339316916225673
50-51	24.155403555080948	25.821340428478056	25.902834790438856	24.120421226002136
52-53	24.351752941840697	25.6166468310871	25.908295860145543	24.123304366926668
54-55	23.96825775790396	25.517451070714337	26.124629238478303	24.3896619329034
56-57	23.98597358136859	25.647523874770055	26.017003830468415	24.34949871339293
58-59	24.088151628013986	25.51311595579974	26.035751290751968	24.362981125434306
60-61	24.08445473500161	25.564558856661918	26.172253588944184	24.178732819392295
62-63	24.089093559613392	25.560293130422814	26.162297517829103	24.18831579213469
64-65	23.924192407865014	25.450301563326192	26.095265782305617	24.53024024650318
66-67	23.933462958242664	25.512850491078982	26.04514120759015	24.508545343088205
68-69	23.97439044530101	25.481271001452615	26.117985051403352	24.426353501843014
70-71	23.941275615669852	25.30290441398975	26.102149401436865	24.653670568903532
72-73	23.891797321433337	25.50574566396383	25.99412639870889	24.608330615893944
74-75	23.901481739777168	25.592806152047725	26.05808010980864	24.447631998366465
76-77	23.950347457143955	25.24790181832582	26.15950814796341	24.642242576566815
78-79	23.84758494321669	25.31220355865848	26.19275284927795	24.647458648846875
80-81	24.0429167934387	25.41716778492945	26.07032820823146	24.469587213400388
82-83	23.993999853072534	25.432273671651384	26.13893017419614	24.434796301079945
84-85	23.970243351505186	25.468666230594643	26.082842354345086	24.478248063555085
86-87	23.886552883513808	25.517059816582016	26.108705800575056	24.487681499329124
88-89	23.82264405545397	25.297491743305617	26.175859899373048	24.70400430186736
90-91	23.819939586885784	25.31020653462993	26.235761742725323	24.634092135758962
92-93	23.975744706376005	25.142095838640433	26.341711887333076	24.540447567650485
94-95	23.859319755719838	25.06734674611541	26.378416786731368	24.694916711433383
96-97	23.888837531712277	24.93130704451562	26.39328195680022	24.786573466971888
98-99	23.734552114395868	24.895857463978054	26.389724949633823	24.979865471992248
100-101	23.939236121599127	24.813228259463134	26.506238556161055	24.741297062776688
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	47.0
1	40.0
2	71.0
3	110.5
4	92.5
5	87.0
6	147.5
7	343.5
8	538.5
9	586.0
10	522.0
11	481.0
12	577.0
13	694.5
14	734.0
15	811.0
16	963.5
17	1064.0
18	1196.5
19	1289.0
20	1351.0
21	1487.0
22	1674.0
23	1876.0
24	2047.5
25	2320.5
26	2815.0
27	3678.0
28	5017.5
29	7171.5
30	10601.5
31	15820.5
32	23167.5
33	30497.5
34	37759.0
35	46266.5
36	57445.0
37	69452.0
38	80412.0
39	91202.0
40	99937.5
41	107935.5
42	116600.5
43	125053.0
44	133516.5
45	142608.0
46	148688.0
47	152695.0
48	157157.0
49	159429.5
50	158389.5
51	156946.5
52	155086.0
53	152621.5
54	149949.0
55	142621.0
56	131575.5
57	118541.5
58	107043.0
59	93957.5
60	77325.0
61	63900.0
62	53090.0
63	40861.5
64	29648.0
65	22010.0
66	16512.5
67	12864.5
68	9886.0
69	7508.0
70	5683.5
71	4144.5
72	2955.5
73	2174.5
74	1665.0
75	1241.0
76	880.5
77	597.5
78	399.5
79	273.0
80	203.5
81	162.5
82	145.0
83	119.5
84	85.5
85	65.5
86	62.0
87	59.5
88	45.5
89	41.5
90	36.5
91	34.0
92	34.5
93	26.5
94	28.5
95	24.0
96	13.0
97	8.5
98	9.5
99	7.5
100	5.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.056003643880732985
2	0.07652149539259312
3	0.0719806594022634
4	0.07270943554885953
5	0.06797239059598473
6	0.010258925755930065
7	0.01348235871202831
8	0.01743456781472268
9	0.0057461196173925234
10-11	0.01349637363792439
12-13	0.022914403840089696
14-15	0.040951613468343785
16-17	0.027567359237588032
18-19	0.010875582495357555
20-21	0.02112049332539154
22-23	0.037405837216635714
24-25	0.034280508741810024
26-27	0.02134473213972881
28-29	0.012277075084965488
30-31	0.011506254160681125
32-33	0.00847903016712799
34-35	0.005465821099470937
36-37	6.166567394274902E-4
38-39	0.0035738061035002276
40-41	0.001303388108335377
42-43	0.22814897866227532
44-45	0.19889982831715777
46-47	0.20540275393293858
48-49	0.21242423180687434
50-51	0.20338460460390317
52-53	0.21089660488420167
54-55	0.20700045548509163
56-57	0.20983147051609966
58-59	0.21811429172068253
60-61	0.2226411127851161
62-63	0.20840194807469956
64-65	0.20819172418625836
66-67	0.20608948530184645
68-69	0.20060964927647945
70-71	0.19311166392207701
72-73	0.20428155986125224
74-75	0.20320241056725413
76-77	0.1973862163203812
78-79	0.2154935005781157
80-81	0.22664938159139483
82-83	0.22551417259381243
84-85	0.21906730668161592
86-87	0.23787533723415438
88-89	0.23097999369328334
90-91	0.2372446655688308
92-93	0.23319435198486388
94-95	0.2363757401632739
96-97	0.2430748747415998
98-99	0.23658596405171506
100-101	0.24297677026032727
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3567625.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.212445030386895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.43319835562451	45.07575802803837
2	12.245773256677122	14.257128051177977
3	4.163110459965265	7.2703451641846994
4	1.9208395702358618	4.4726707157818835
5	1.0980043909791042	3.195876012649727
6	0.6863418734751418	2.397218314903467
7	0.45622778830080063	1.8590694533456742
8	0.33091681901951625	1.5410781709443262
9	0.2510421323430752	1.315239869639914
>10	1.3318840885233134	13.61606385439458
>50	0.0629913132663819	2.4641362627491845
>100	0.018124576731776944	1.6850968567041453
>500	0.00101415225778895	0.41266361305834237
>1k	5.312226003679432E-4	0.4376556324276648
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	5.60597035843173E-5	0.0
3	0.0	0.0	0.0	5.60597035843173E-5	0.0
4	0.0	0.0	0.0	5.60597035843173E-5	0.0
5	0.0	0.0	0.0	5.60597035843173E-5	0.0
6	0.0	0.0	0.0	5.60597035843173E-5	2.802985179215865E-5
7	0.0	0.0	0.0	5.60597035843173E-5	2.802985179215865E-5
8	0.0	0.0	0.0	5.60597035843173E-5	2.802985179215865E-5
9	0.0	0.0	0.0	8.408955537647594E-5	2.802985179215865E-5
10-11	0.0	2.802985179215865E-5	0.0	8.408955537647594E-5	2.802985179215865E-5
12-13	0.0	2.802985179215865E-5	0.0	1.121194071686346E-4	2.1022388844118986E-4
14-15	0.0	2.802985179215865E-5	0.0	1.121194071686346E-4	2.6628359202550715E-4
16-17	0.0	2.802985179215865E-5	0.0	1.5416418485687258E-4	3.924179250902211E-4
18-19	0.0	2.802985179215865E-5	0.0	1.9620896254511055E-4	4.484776286745384E-4
20-21	0.0	2.802985179215865E-5	0.0	2.1022388844118986E-4	4.7650748046669706E-4
22-23	0.0	2.802985179215865E-5	0.0	2.5226866612942787E-4	5.60597035843173E-4
24-25	0.0	5.60597035843173E-5	0.0	3.223432956098245E-4	5.60597035843173E-4
26-27	0.0	5.60597035843173E-5	0.0	6.867313689078869E-4	5.886268876353316E-4
28-29	0.0	5.60597035843173E-5	0.0	0.002032164254931502	5.886268876353316E-4
30-31	0.0	5.60597035843173E-5	0.0	0.009305910794996671	5.886268876353316E-4
32-33	0.0	5.60597035843173E-5	0.0	0.02881468764233909	5.886268876353316E-4
34-35	0.0	5.60597035843173E-5	0.0	0.054784345327774084	7.007462948039662E-4
36-37	0.0	5.60597035843173E-5	0.0	0.09633860060964927	7.007462948039662E-4
38-39	0.0	5.60597035843173E-5	0.0	0.1680529764198872	7.147612207000455E-4
40-41	0.0	5.60597035843173E-5	0.0	0.2523387407589083	7.568059983882835E-4
42-43	1.4014925896079325E-5	8.408955537647594E-5	0.0	0.3407028485336884	7.848358501804421E-4
44-45	2.802985179215865E-5	8.408955537647594E-5	0.0	0.44978101678287374	8.549104796608388E-4
46-47	2.802985179215865E-5	8.408955537647594E-5	0.0	0.5772467678077152	9.249851091412355E-4
48-49	2.802985179215865E-5	8.408955537647594E-5	0.0	0.7112715041519218	9.53014960933394E-4
50-51	2.802985179215865E-5	8.408955537647594E-5	0.0	0.8520234049262465	9.53014960933394E-4
52-53	2.802985179215865E-5	8.408955537647594E-5	0.0	1.0016327388668933	0.0010511194422059493
54-55	2.802985179215865E-5	8.408955537647594E-5	0.0	1.1673312077362392	0.0010651343681020286
56-57	2.802985179215865E-5	8.408955537647594E-5	0.0	1.3567008864440628	0.0011071791457902666
58-59	2.802985179215865E-5	8.408955537647594E-5	0.0	1.5631407448933115	0.001121194071686346
60-61	2.802985179215865E-5	8.408955537647594E-5	0.0	1.78691706667601	0.001121194071686346
62-63	2.802985179215865E-5	8.408955537647594E-5	0.0	2.016327388668932	0.001121194071686346
64-65	2.802985179215865E-5	8.408955537647594E-5	0.0	2.261981009775411	0.0011772537752706633
66-67	2.802985179215865E-5	8.408955537647594E-5	0.0	2.551781647454539	0.001205283627062822
68-69	2.802985179215865E-5	1.121194071686346E-4	0.0	2.864398584492484	0.00124732840475106
70-71	2.802985179215865E-5	1.4014925896079324E-4	0.0	3.197813671560212	0.0012613433306471393
72-73	2.802985179215865E-5	1.6817911075295188E-4	0.0	3.5439402964156828	0.0013174030342314566
74-75	2.802985179215865E-5	1.6817911075295188E-4	0.0	3.907571563715357	0.0013734627378157737
76-77	2.802985179215865E-5	1.9620896254511055E-4	0.0	4.311509757892155	0.001387477663711853
78-79	2.802985179215865E-5	1.9620896254511055E-4	0.0	4.763421043411233	0.0014014925896079324
80-81	2.802985179215865E-5	1.9620896254511055E-4	0.0	5.238078553659648	0.0014435373672961704
82-83	2.802985179215865E-5	1.9620896254511055E-4	0.0	5.728586945096527	0.0016257314039452016
84-85	2.802985179215865E-5	1.9620896254511055E-4	0.0	6.239445008934515	0.0016257314039452016
86-87	2.802985179215865E-5	1.9620896254511055E-4	0.0	6.7874005816194245	0.0016677761816334396
88-89	2.802985179215865E-5	1.9620896254511055E-4	0.0	7.377456991696157	0.0017098209593216776
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4900	0.0	44.16744	1
GGTATCA	2660	0.0	43.535313	1
TCAACGC	5780	0.0	36.215878	4
ATCAACG	6030	0.0	34.791645	3
CAACGCA	6010	0.0	34.670498	5
TATCAAC	6215	0.0	33.833332	2
AACGCAG	6585	0.0	31.839668	6
GTACATG	9680	0.0	28.044914	1
ACGCAGA	7450	0.0	27.447845	7
TACATGG	9855	0.0	26.779373	2
CGCAGAG	7755	0.0	25.39018	8
ACATGGG	10355	0.0	24.247982	3
CATGGGG	9100	0.0	22.011993	4
GCAGAGT	8980	0.0	20.341825	9
AGAGTAC	7670	0.0	18.558142	10-11
GTATAGA	1055	0.0	18.444414	1
ATGGGGG	5330	0.0	17.899416	5
GTATAAC	855	0.0	17.76304	1
GTATTAG	955	0.0	17.393946	1
TAAGGTG	1710	0.0	17.209394	5
>>END_MODULE