FastQCFastQC Report
Mon 15 Aug 2016
SRR2921692_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921692_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3567625
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23850.045.4453541
GTATCAA46100.042.382241
TCAACGC55500.034.207514
CAACGCA56550.033.5992055
ATCAACG59150.032.177343
TATCAAC61250.031.2291032
AACGCAG61800.031.2012636
GTACATG99550.027.8400671
TACATGG103750.026.3051192
ACGCAGA74300.025.440697
ACATGGG106950.025.0742633
CGCAGAG77000.024.3635738
CATGGGG89600.022.9898514
ATGGGGG51700.019.5699945
GCAGAGT90500.019.4180689
AGAGTAC74200.018.78698310-11
GTACCGT4850.018.6057196
TACCGTA2603.2895878E-718.2667487
GAGTACA76850.016.454351
TAAGGTG17100.016.1114085
Produced by FastQC (version 0.11.5)