##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921692_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3567625
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.35138082057391	33.0	31.0	34.0	28.0	34.0
2	31.48904243018815	34.0	31.0	34.0	28.0	34.0
3	31.561590974387723	34.0	31.0	34.0	28.0	34.0
4	35.034628078904035	37.0	35.0	37.0	32.0	37.0
5	34.77014680634876	37.0	35.0	37.0	32.0	37.0
6	34.743319995795524	37.0	35.0	37.0	32.0	37.0
7	34.628608948530186	37.0	35.0	37.0	32.0	37.0
8	34.57457243964823	37.0	35.0	37.0	32.0	37.0
9	36.158950282050384	39.0	37.0	39.0	32.0	39.0
10-11	36.05016138187169	39.0	37.0	39.0	31.5	39.0
12-13	35.97124753862864	39.0	37.0	39.0	31.0	39.0
14-15	37.2111647104166	40.0	38.0	41.0	31.0	41.0
16-17	37.17756742931222	40.0	38.0	41.0	31.0	41.0
18-19	37.13688434182404	40.0	38.0	41.0	31.0	41.0
20-21	37.07752944886304	40.0	38.0	41.0	31.0	41.0
22-23	36.98569005991381	40.0	37.5	41.0	31.0	41.0
24-25	36.89930738236222	40.0	37.0	41.0	30.0	41.0
26-27	36.775298272660386	40.0	37.0	41.0	30.0	41.0
28-29	36.65502680354578	40.0	37.0	41.0	30.0	41.0
30-31	36.501442836620996	40.0	37.0	41.0	30.0	41.0
32-33	36.45318173855156	40.0	37.0	41.0	30.0	41.0
34-35	36.58475568480432	40.0	37.0	41.0	30.0	41.0
36-37	36.585462037069476	40.0	37.0	41.0	30.0	41.0
38-39	36.48029277180197	40.0	37.0	41.0	30.0	41.0
40-41	36.4006116113661	40.0	37.0	41.0	30.0	41.0
42-43	36.28416663746891	40.0	36.5	41.0	29.0	41.0
44-45	36.15381465260502	40.0	36.0	41.0	28.5	41.0
46-47	36.00432276374339	40.0	36.0	41.0	28.0	41.0
48-49	35.86471041659367	40.0	35.5	41.0	28.0	41.0
50-51	35.711545916400965	40.0	35.0	41.0	27.5	41.0
52-53	35.53705252093479	39.0	35.0	41.0	27.0	41.0
54-55	35.320991556007144	39.0	35.0	41.0	26.5	41.0
56-57	35.095058196979785	39.0	35.0	41.0	26.0	41.0
58-59	34.85458869696227	38.5	35.0	40.5	26.0	41.0
60-61	34.57615808836411	38.0	34.5	40.0	26.0	41.0
62-63	34.27196650432711	37.5	34.0	40.0	25.5	41.0
64-65	33.93565999789776	37.0	34.0	40.0	24.5	41.0
66-67	33.58112792123612	36.5	34.0	39.0	23.0	41.0
68-69	33.2219636312673	36.0	34.0	39.0	23.0	41.0
70-71	32.82226733471147	35.0	33.0	38.5	22.0	40.0
72-73	32.46377211730493	35.0	33.0	37.5	21.5	40.0
74-75	32.060717283907366	35.0	33.0	37.0	20.5	39.0
76-77	29.36393258820644	33.0	29.0	34.5	13.5	36.5
78-79	30.93109267369749	34.5	31.5	36.0	17.5	37.0
80-81	31.113937563505132	35.0	32.0	35.5	18.0	37.0
82-83	30.94477054062577	35.0	32.0	35.0	18.0	37.0
84-85	30.748994358992327	35.0	32.0	35.0	16.0	36.0
86-87	30.54473101853474	35.0	32.0	35.0	11.5	36.0
88-89	30.278712729056444	35.0	32.0	35.0	7.0	36.0
90-91	30.087046704740548	34.5	31.5	35.0	2.0	35.0
92-93	29.934791913387755	34.0	31.0	35.0	2.0	35.0
94-95	29.834784765775552	34.0	31.0	35.0	2.0	35.0
96-97	29.638208051574928	34.0	31.0	35.0	2.0	35.0
98-99	29.44265526786027	34.0	31.0	35.0	2.0	35.0
100-101	28.335338775796224	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	69677.0
3	24154.0
4	12999.0
5	9098.0
6	7819.0
7	7804.0
8	8309.0
9	8877.0
10	9572.0
11	10430.0
12	11045.0
13	11702.0
14	12616.0
15	13346.0
16	14056.0
17	14948.0
18	14214.0
19	12899.0
20	12633.0
21	12499.0
22	13205.0
23	15265.0
24	17910.0
25	21685.0
26	25990.0
27	31310.0
28	37850.0
29	45622.0
30	55430.0
31	69506.0
32	88432.0
33	116431.0
34	166105.0
35	249386.0
36	441906.0
37	859171.0
38	905342.0
39	108381.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.77025638808398	19.446340319167664	13.648276572669026	26.13512672007933
2	14.909892258644284	20.790314433888383	38.003283983879435	26.296509323587898
3	17.669407628701077	24.995017154723865	28.99926245480048	28.336312761774586
4	13.099876297259955	16.979091069379773	35.47642093466348	34.44461169869679
5	14.328607448574566	37.23342423981592	34.227622467910535	14.210345843698983
6	33.49083563111779	35.62596021322094	17.0501352882791	13.833068867382167
7	28.918734452191586	31.642030762762342	21.20421849269472	18.235016292351354
8	26.594274902771453	35.03465190427806	19.80861217196314	18.56246102098735
9	26.706366280088297	15.749160856311972	19.17833292456466	38.36613993903507
10-11	25.61777328334303	25.30929502482058	27.836376962114525	21.236554729721867
12-13	26.18839863931544	22.666915385512343	27.642492914035593	23.502193061136627
14-15	23.409043287892413	24.17031481147962	24.701688389684236	27.718953510943734
16-17	23.75219140915755	27.231467325106664	25.479293014161104	23.53704825157468
18-19	23.785102796002494	26.713660299550774	26.610158635745762	22.89107826870097
20-21	24.324646290011355	25.909768214704965	26.481621319807196	23.28396417547648
22-23	24.16861357345573	25.633621807224692	26.242191934410148	23.95557268490943
24-25	24.016016358324674	25.876901925032286	26.14053835874372	23.96654335789932
26-27	23.808425944119865	26.01051035604756	26.218413361180804	23.962650338651766
28-29	23.92455067412022	26.160994161759422	26.07099934056123	23.843455823559133
30-31	24.22154864371304	25.926754504743176	25.862905653966422	23.98879119757736
32-33	24.00635733949911	25.99670527334275	25.77440303228106	24.222534354877073
34-35	24.268568513976447	26.029649856746133	25.887748567286145	23.814033061991267
36-37	24.33646780591171	25.834346069823244	25.9448442520833	23.884341872181746
38-39	24.179461126099298	25.9242212956799	25.879990189551872	24.01632738866893
40-41	24.32489782051674	25.806833194253564	25.93430351868551	23.93396546654419
42-43	24.198643959277852	25.781340886391014	25.77108101736413	24.24893413696701
44-45	24.063728535902314	25.780842334917082	25.924505130724036	24.23092399845657
46-47	24.247930196261347	25.79502720121273	25.729364751892646	24.227677850633274
48-49	24.02460807718141	25.898148283119593	25.789984761516045	24.287258878182953
50-51	24.093155938566348	25.944061756005247	25.806403237427865	24.156379068000533
52-53	24.315850801312887	25.724253735370045	25.7850237060348	24.174871757282272
54-55	23.84556702756136	25.67227842986492	26.013668204962247	24.468486337611477
56-57	23.9002099189912	25.783804323038932	25.90166563017676	24.41432012779311
58-59	24.02368205891163	25.652209077515174	25.918869128141903	24.40523973543129
60-61	23.97146351120751	25.767019751987636	26.01932099695503	24.24219573984982
62-63	23.99125731104	25.710217316826594	25.99778068478042	24.30074468735299
64-65	23.748439150494075	25.60927460772308	25.932450723704193	24.709835518078656
66-67	23.79925477586692	25.675931694939663	25.87710939644025	24.647704132753162
68-69	23.835699916331674	25.64367612855841	25.904440310494042	24.616183644615873
70-71	23.776159636418868	25.489961787573577	25.88324951619144	24.850629059816114
72-73	23.753514351958685	25.669566938748844	25.71663058500519	24.86028812428728
74-75	23.689036665197502	25.756325540030257	25.775951788960626	24.77868600581161
76-77	23.72734199021339	25.39101764882044	25.967198736472337	24.914441624493826
78-79	23.621521076612094	25.465444977194668	25.952200041154384	24.96083390503886
80-81	23.73603719208196	25.551675536950164	25.810814032578133	24.901473238389745
82-83	23.677442417955223	25.57259613362679	25.86579843831046	24.884163010107525
84-85	23.585824238846488	25.565966170331805	25.837069048924437	25.01114054189727
86-87	23.49012800274652	25.655678894749183	25.81406953144196	25.040123571062335
88-89	23.39911912651445	25.43077311027842	25.87607329573583	25.2940344674713
90-91	23.335172910922086	25.426879461655066	25.913838046700345	25.324109580722503
92-93	23.412297832284494	25.29701604861911	25.990010260089125	25.300675859007278
94-95	23.247946578883592	25.267567418691865	25.993386835370437	25.4910991670541
96-97	23.157430257713767	25.06758296732609	26.099738807214944	25.6752479677452
98-99	22.962650894699244	25.067205629113502	26.034383662609734	25.93575981357752
100-101	22.979334813966233	25.034823650566896	26.067459286916467	25.918382248550397
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	46.0
1	36.5
2	50.5
3	72.0
4	66.0
5	85.5
6	129.0
7	280.5
8	438.0
9	464.5
10	402.5
11	340.0
12	344.5
13	375.5
14	402.5
15	424.5
16	478.0
17	540.5
18	619.5
19	719.0
20	805.5
21	906.5
22	1084.5
23	1321.0
24	1607.5
25	2007.5
26	2665.5
27	3601.0
28	4999.5
29	7345.5
30	11017.0
31	16441.5
32	23953.5
33	31069.0
34	38156.5
35	47099.0
36	57972.5
37	69241.5
38	80307.0
39	90801.5
40	99344.5
41	107147.0
42	115874.5
43	125504.0
44	134484.5
45	142871.5
46	148599.5
47	153450.5
48	158430.5
49	160254.0
50	158777.5
51	157367.0
52	156476.5
53	155166.5
54	152292.5
55	144221.5
56	133249.0
57	119933.5
58	107760.0
59	93839.0
60	76741.5
61	63441.0
62	52135.5
63	40011.0
64	28805.5
65	21062.5
66	15759.0
67	12146.5
68	9290.5
69	6935.0
70	5241.5
71	3827.0
72	2645.0
73	1879.5
74	1369.5
75	973.0
76	663.0
77	391.0
78	204.0
79	123.0
80	81.5
81	52.0
82	32.0
83	18.5
84	13.5
85	12.0
86	7.5
87	6.0
88	5.5
89	3.0
90	3.0
91	2.0
92	1.0
93	0.5
94	0.5
95	1.5
96	1.5
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16251708069093584
2	1.9620896254511053E-4
3	0.010819522791773239
4	0.005662030062016047
5	0.02679653831330367
6	0.0016257314039452016
7	0.0
8	0.0
9	0.0
10-11	0.04858974808170702
12-13	2.5226866612942787E-4
14-15	0.004835149434147367
16-17	1.4014925896079323E-5
18-19	0.001387477663711853
20-21	3.6438807329806244E-4
22-23	0.0
24-25	6.306716653235695E-4
26-27	0.0026908657720472306
28-29	0.005535895728951333
30-31	0.0170141200378403
32-33	0.011478224308888967
34-35	0.009992642163904558
36-37	0.004078343435759083
38-39	0.0
40-41	0.0035878210293963067
42-43	0.009193791387828037
44-45	0.006825268911390631
46-47	0.0038400896955257348
48-49	0.009558179461126099
50-51	0.0028450299569041026
52-53	0.0020601941067236607
54-55	0.00416243299113556
56-57	0.00831085105637504
58-59	0.015038015486493117
60-61	0.0073578360954416454
62-63	0.021274657510248415
64-65	0.04086752391296731
66-67	0.05943730072527242
68-69	0.04931852422830314
70-71	0.06793034581829649
72-73	0.033019165411162885
74-75	0.027273045793770365
76-77	0.22611681440734382
78-79	0.0840475105987877
80-81	0.06587015171157282
82-83	0.02732910549735468
84-85	0.05101433026172873
86-87	0.06696331593146701
88-89	0.29358466767106967
90-91	0.2984898917346975
92-93	0.05231771837006412
94-95	0.009712343645982972
96-97	0.014799761746259767
98-99	8.268806278686802E-4
100-101	1.8219403664903122E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3567625.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.27797753274471
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.68989064849133	43.92641833552935
2	12.564260047678802	14.393108094530168
3	4.34012657775676	7.457810178300598
4	2.003742767319589	4.590813328317245
5	1.1493076926795862	3.2915010099756
6	0.6918845572323404	2.377784887464421
7	0.48076341170008846	1.9276009125746354
8	0.3472203393793697	1.5910463038306826
9	0.25427362453757174	1.3107851058089341
>10	1.3932626591574608	14.08808276802306
>50	0.06491082383331807	2.4911344974887553
>100	0.01873625009647268	1.7043154344161735
>500	0.001080400096868505	0.42980648222172874
>1k	5.402000404132007E-4	0.4197926615186363
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.802985179215865E-5
2	0.0	0.0	0.0	0.0	2.802985179215865E-5
3	0.0	0.0	0.0	0.0	2.802985179215865E-5
4	0.0	0.0	0.0	2.802985179215865E-5	2.802985179215865E-5
5	0.0	0.0	0.0	2.802985179215865E-5	2.802985179215865E-5
6	0.0	0.0	0.0	2.802985179215865E-5	8.408955537647594E-5
7	0.0	0.0	0.0	2.802985179215865E-5	8.408955537647594E-5
8	0.0	0.0	0.0	2.802985179215865E-5	8.408955537647594E-5
9	0.0	0.0	0.0	5.60597035843173E-5	1.121194071686346E-4
10-11	0.0	0.0	0.0	5.60597035843173E-5	1.121194071686346E-4
12-13	0.0	0.0	0.0	5.60597035843173E-5	1.8219403664903122E-4
14-15	0.0	1.4014925896079325E-5	0.0	5.60597035843173E-5	2.802985179215865E-4
16-17	0.0	2.802985179215865E-5	0.0	8.408955537647596E-5	3.924179250902211E-4
18-19	0.0	2.802985179215865E-5	0.0	1.4014925896079324E-4	3.924179250902211E-4
20-21	0.0	2.802985179215865E-5	0.0	1.4014925896079324E-4	4.064328509863004E-4
22-23	0.0	5.60597035843173E-5	0.0	1.6817911075295188E-4	4.6249255457061773E-4
24-25	0.0	5.60597035843173E-5	0.0	2.3825374023334853E-4	5.045373322588557E-4
26-27	0.0	5.60597035843173E-5	0.0	5.746119617392523E-4	5.045373322588557E-4
28-29	0.0	5.60597035843173E-5	0.0	0.001962089625451105	5.045373322588557E-4
30-31	0.0	5.60597035843173E-5	0.0	0.009305910794996671	5.045373322588557E-4
32-33	0.0	5.60597035843173E-5	0.0	0.029123016012052836	5.045373322588557E-4
34-35	0.0	5.60597035843173E-5	0.0	0.05561122595564276	5.045373322588557E-4
36-37	0.0	7.007462948039662E-5	0.0	0.09709540660803756	5.045373322588557E-4
38-39	0.0	8.408955537647594E-5	0.0	0.1693984093059108	5.18552258154935E-4
40-41	0.0	8.408955537647594E-5	0.0	0.2545250691986966	5.60597035843173E-4
42-43	0.0	1.4014925896079324E-4	0.0	0.34329560982446305	5.886268876353316E-4
44-45	0.0	1.4014925896079324E-4	0.0	0.453172628849725	5.886268876353316E-4
46-47	0.0	1.4014925896079324E-4	0.0	0.5810167828737606	6.306716653235696E-4
48-49	0.0	1.4014925896079324E-4	0.0	0.7150835639956554	7.007462948039662E-4
50-51	0.0	1.4014925896079324E-4	0.0	0.8558074349181879	7.568059983882835E-4
52-53	0.0	1.4014925896079324E-4	0.0	1.0048561718229916	8.268806278686801E-4
54-55	0.0	1.6817911075295188E-4	0.0	1.1705406257664412	8.689254055569181E-4
56-57	0.0	1.6817911075295188E-4	0.0	1.3611996776567046	8.689254055569181E-4
58-59	0.0	1.6817911075295188E-4	0.0	1.5679618794015626	8.969552573490768E-4
60-61	0.0	1.6817911075295188E-4	0.0	1.7921866788129357	8.969552573490768E-4
62-63	0.0	1.6817911075295188E-4	0.0	2.021372761991521	9.810448127255528E-4
64-65	0.0	1.6817911075295188E-4	0.0	2.268175607021478	9.810448127255528E-4
66-67	0.0	1.6817911075295188E-4	0.0	2.558424722329281	9.810448127255528E-4
68-69	0.0	1.6817911075295188E-4	0.0	2.870915525034161	9.810448127255528E-4
70-71	0.0	1.6817911075295188E-4	0.0	3.204386671805473	0.00103710451630987
72-73	0.0	1.6817911075295188E-4	0.0	3.551017833993203	0.0011912687011667426
74-75	0.0	1.6817911075295188E-4	0.0	3.9168354297326653	0.0012613433306471393
76-77	0.0	1.6817911075295188E-4	0.0	4.323268280718965	0.0012613433306471393
78-79	0.0	1.6817911075295188E-4	0.0	4.777029536456325	0.0012613433306471393
80-81	0.0	1.6817911075295188E-4	0.0	5.254504046809853	0.0013174030342314566
82-83	0.0	1.6817911075295188E-4	0.0	5.746554080095302	0.001429522441400091
84-85	0.0	1.6817911075295188E-4	0.0	6.26137836796188	0.001429522441400091
86-87	0.0	1.6817911075295188E-4	0.0	6.813258119897691	0.0014575522931922498
88-89	0.0	1.6817911075295188E-4	0.0	7.408682246592621	0.0014855821449844084
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2385	0.0	45.445354	1
GTATCAA	4610	0.0	42.38224	1
TCAACGC	5550	0.0	34.20751	4
CAACGCA	5655	0.0	33.599205	5
ATCAACG	5915	0.0	32.17734	3
TATCAAC	6125	0.0	31.229103	2
AACGCAG	6180	0.0	31.201263	6
GTACATG	9955	0.0	27.840067	1
TACATGG	10375	0.0	26.305119	2
ACGCAGA	7430	0.0	25.44069	7
ACATGGG	10695	0.0	25.074263	3
CGCAGAG	7700	0.0	24.363573	8
CATGGGG	8960	0.0	22.989851	4
ATGGGGG	5170	0.0	19.569994	5
GCAGAGT	9050	0.0	19.418068	9
AGAGTAC	7420	0.0	18.786983	10-11
GTACCGT	485	0.0	18.605719	6
TACCGTA	260	3.2895878E-7	18.266748	7
GAGTACA	7685	0.0	16.45435	1
TAAGGTG	1710	0.0	16.111408	5
>>END_MODULE