FastQCFastQC Report
Mon 15 Aug 2016
SRR2921691_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921691_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028891
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA28300.057.6966171
GTATCAA57300.044.3177261
TCAACGC70050.035.714574
CAACGCA71550.034.8296855
ATCAACG72750.034.519023
TATCAAC75300.033.4141962
AACGCAG75650.033.2354356
GTACATG113500.030.1102941
TACATGG118350.028.3997882
ACGCAGA88500.027.7724487
CGCAGAG90750.026.8233348
ACATGGG123050.026.5425453
CATGGGG97250.024.1634254
GCAGAGT101400.022.9777289
ATGGGGG59950.020.9824685
AGAGTAC88250.019.68021810-11
CAGAGTA95400.019.34927710-11
ACGTATA2302.0711923E-618.5765382
GAGTACA85300.018.0847571
GTATAGA8950.018.0315971
Produced by FastQC (version 0.11.5)