##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921691_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3028891
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.313460603237292	31.0	31.0	34.0	26.0	34.0
2	30.367895708363225	31.0	31.0	34.0	26.0	34.0
3	30.42577695928972	33.0	31.0	34.0	26.0	34.0
4	33.886304921504276	37.0	35.0	37.0	30.0	37.0
5	33.88969064915179	37.0	35.0	37.0	30.0	37.0
6	33.932204559358524	37.0	35.0	37.0	30.0	37.0
7	33.84529948420065	37.0	35.0	37.0	29.0	37.0
8	33.81989414607525	37.0	35.0	37.0	29.0	37.0
9	35.36259475827951	39.0	36.0	39.0	29.0	39.0
10-11	35.255819209076854	39.0	35.0	39.0	29.0	39.0
12-13	35.1439592907107	39.0	35.0	39.0	28.0	39.0
14-15	36.26193960099587	40.0	36.0	41.0	27.5	41.0
16-17	36.19817814507026	40.0	36.0	41.0	27.0	41.0
18-19	36.173082986479216	40.0	36.5	41.0	27.0	41.0
20-21	36.10057278390012	40.0	36.0	41.0	27.0	41.0
22-23	36.00401830240837	40.0	36.0	41.0	27.0	41.0
24-25	35.88663804673064	40.0	36.0	41.0	27.0	41.0
26-27	35.73509231596647	40.0	36.0	41.0	27.0	41.0
28-29	35.598655250387026	40.0	36.0	41.0	26.0	41.0
30-31	35.441240374777436	40.0	35.5	41.0	25.0	41.0
32-33	35.25730457121105	39.0	35.0	41.0	25.0	41.0
34-35	35.083677656277494	39.0	35.0	41.0	24.0	41.0
36-37	35.044193402799905	39.0	35.0	41.0	24.0	41.0
38-39	35.2142185043965	39.5	35.0	41.0	24.5	41.0
40-41	35.27208027624633	40.0	35.0	41.0	24.0	41.0
42-43	35.111506653755455	40.0	35.0	41.0	24.0	41.0
44-45	34.987517378472845	40.0	35.0	41.0	23.0	41.0
46-47	34.821444713593195	39.5	35.0	41.0	22.5	41.0
48-49	34.65331601566382	39.0	35.0	41.0	21.0	41.0
50-51	32.969064254870844	37.0	32.5	39.5	17.0	40.0
52-53	33.0878423819147	37.5	32.5	39.5	16.5	40.0
54-55	33.99261495379002	38.5	34.0	40.5	18.0	41.0
56-57	34.019934028659335	39.0	34.0	41.0	17.5	41.0
58-59	33.83455149095825	38.0	34.0	41.0	15.5	41.0
60-61	33.58102619077411	38.0	34.0	40.0	13.0	41.0
62-63	33.29259009320573	37.0	33.0	40.0	11.5	41.0
64-65	33.0124165907588	37.0	33.0	40.0	8.0	41.0
66-67	32.6813087694473	36.0	33.0	39.5	7.0	41.0
68-69	32.329874036404746	36.0	33.0	39.0	4.0	41.0
70-71	31.928386495255193	35.0	32.0	39.0	2.0	40.5
72-73	31.57408272532752	35.0	32.0	37.5	2.0	40.0
74-75	31.181768673748905	35.0	32.0	37.0	2.0	39.0
76-77	30.816588645811287	35.0	31.5	36.5	2.0	39.0
78-79	30.476724649384874	35.0	31.0	36.0	2.0	38.5
80-81	30.164014981060724	35.0	31.0	36.0	2.0	37.0
82-83	29.85645175082233	35.0	31.0	35.0	2.0	37.0
84-85	29.59330197091939	35.0	31.0	35.0	2.0	36.5
86-87	29.362201379977027	35.0	31.0	35.0	2.0	36.0
88-89	29.14236514288563	35.0	30.0	35.0	2.0	36.0
90-91	28.915094336507984	34.0	30.0	35.0	2.0	35.5
92-93	28.72265624613101	34.0	30.0	35.0	2.0	35.0
94-95	28.490990266734592	34.0	30.0	35.0	2.0	35.0
96-97	28.25444642940271	34.0	29.5	35.0	2.0	35.0
98-99	27.989021889529866	34.0	29.0	35.0	2.0	35.0
100-101	26.92136891027112	33.0	26.5	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	106096.0
3	18342.0
4	12481.0
5	9627.0
6	8583.0
7	9024.0
8	9596.0
9	10097.0
10	11092.0
11	11865.0
12	12027.0
13	12124.0
14	12718.0
15	13787.0
16	14089.0
17	14673.0
18	13592.0
19	13011.0
20	12940.0
21	13668.0
22	14833.0
23	17257.0
24	19707.0
25	23422.0
26	27781.0
27	33261.0
28	38679.0
29	45797.0
30	55000.0
31	66371.0
32	83500.0
33	107107.0
34	150817.0
35	215699.0
36	349989.0
37	632760.0
38	681525.0
39	125936.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.61803135399331	18.07449562934926	12.831465514108901	24.476007502548526
2	15.972084097927022	19.983466934860914	38.78137036775166	25.2630785994604
3	18.819893958096003	24.62497455998689	29.531593192384648	27.023538289532457
4	13.355255376495068	15.97807046814674	36.99108559376183	33.675588561596356
5	14.060307686615166	37.80051676445063	34.11867742394804	14.020498124986167
6	32.72007462255351	35.46212327579802	17.655068802984903	14.162733298663563
7	28.629378701956696	32.25758519329588	21.26499026239687	17.848045842350547
8	27.060862306263754	33.987552272793316	20.411749788002947	18.539835632939983
9	27.48319399870571	15.213954594080588	19.464122984270375	37.838728422943326
10-11	25.62467919291252	25.231084289570283	27.990277017178006	21.153959500339194
12-13	26.180986424441322	22.810419194640247	27.69010099895994	23.31849338195849
14-15	23.53080285394002	24.224222951348132	25.11228091267422	27.13269328203763
16-17	23.559934248059186	27.200332887776696	25.894095122351946	23.345637741812176
18-19	23.58511420734219	26.711269343762822	27.127007017286083	22.576609431608908
20-21	24.214945365788097	25.912131867747217	26.96449614161719	22.9084266248475
22-23	24.150480446654296	25.631329874555593	26.665363170837274	23.552826507952837
24-25	23.822798894470555	25.846514902139972	26.663838496395403	23.666847706994073
26-27	23.490082261733715	26.12466362707838	26.652937114733504	23.7323169964544
28-29	23.64567714574302	26.40002139642726	26.452389812527016	23.50191164530271
30-31	24.108178394533724	26.045798718819285	26.256214020345485	23.589808866301507
32-33	23.816647100611018	26.104942846260993	26.12830324739292	23.950106805735068
34-35	24.1118560506509	26.246940964073413	26.30515014203964	23.336052843236047
36-37	24.21074578258837	25.958888325422798	26.34875293508607	23.48161295690277
38-39	24.01572255404009	26.15439600894092	26.196195155128983	23.63368628189001
40-41	24.115676113090316	25.964709088769066	26.272482310550842	23.647132487589776
42-43	23.91160652461124	25.933501381975336	26.143449122542865	24.011442970870558
44-45	23.743331447152674	25.923515898088024	26.44282482487218	23.890327829887127
46-47	23.986551702747967	25.904844612617083	26.076347621115314	24.032256063519633
48-49	23.696778271617355	26.029363814890612	26.20155790239181	24.07230001110022
50-51	23.85507973720937	26.021430103875314	26.314081351819834	23.809408807095487
52-53	24.034603776175537	25.867670911769302	26.30499064930467	23.792734662750487
54-55	23.424525628633972	25.755329301160867	26.651724811590533	24.168420258614628
56-57	23.544597708146682	25.89238095742245	26.375370053981236	24.187651280449636
58-59	23.696122559756102	25.763595476173577	26.45809127559815	24.08219068847217
60-61	23.788947916132415	25.85248226011657	26.609312260000763	23.749257563750252
62-63	23.714659568942274	25.71264803238309	26.672197289388315	23.900495109286318
64-65	23.41721603650423	25.53643375767646	26.64294831457105	24.40340189124826
66-67	23.470123021393515	25.714531478225194	26.47530556846735	24.340039931913935
68-69	23.565928555584613	25.66698872082635	26.521234484705904	24.245848238883134
70-71	23.453363135691475	25.513797925059883	26.499945253548844	24.532893685699797
72-73	23.414494228464218	25.814633310384593	26.375559561567734	24.39531289958345
74-75	23.44123398143417	25.871889187940532	26.413341566581046	24.27353526404426
76-77	23.460599729134096	25.406480800873865	26.522611745417805	24.61030772457423
78-79	23.407609382558213	25.495405267514876	26.603226470317704	24.493758879609207
80-81	23.61932368588463	25.656886029895343	26.36779595257181	24.355994331648215
82-83	23.631756630993085	25.628429449608753	26.522946549186777	24.216867370211386
84-85	23.526287873699353	25.735735209110832	26.431227208611862	24.30674970857795
86-87	23.41425549662709	25.854843804631813	26.372789555105435	24.358111143635668
88-89	23.38241360156975	25.50607046293764	26.52942127376392	24.582094661728693
90-91	23.454002860988872	25.410578378105576	26.66899980391719	24.466418956988367
92-93	23.711698254069475	25.18638558726228	26.71772738809998	24.38418877056827
94-95	23.532743525664777	25.1586555270931	26.761881769634805	24.54671917760732
96-97	23.435909579602374	25.017652606305756	26.820096650398302	24.726341163693565
98-99	23.265769772042272	24.936439398389496	26.782853157429248	25.014937672138988
100-101	23.43528282606807	24.968488463972925	26.8694569358889	24.726771774070105
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	64.0
1	59.0
2	109.0
3	175.0
4	151.0
5	160.0
6	269.0
7	506.0
8	782.0
9	860.0
10	716.5
11	656.0
12	734.0
13	793.5
14	873.0
15	947.0
16	1079.0
17	1217.0
18	1288.5
19	1410.0
20	1551.0
21	1639.5
22	1822.0
23	2049.5
24	2295.0
25	2759.5
26	3697.0
27	5023.0
28	6966.0
29	10183.0
30	14737.5
31	20630.0
32	28703.5
33	35180.0
34	40252.5
35	47370.0
36	56110.0
37	65175.0
38	72829.0
39	79938.5
40	85467.0
41	90355.5
42	95746.0
43	101147.0
44	107144.0
45	114517.5
46	120197.5
47	124067.0
48	128791.5
49	131873.5
50	132451.5
51	130946.5
52	128307.5
53	126368.0
54	124004.5
55	118698.0
56	109728.5
57	98382.5
58	88216.5
59	76682.5
60	63006.5
61	52084.0
62	43506.0
63	33692.0
64	24012.5
65	17501.0
66	12807.0
67	9518.5
68	7221.0
69	5350.5
70	3977.5
71	2902.5
72	2108.0
73	1579.0
74	1229.0
75	927.0
76	681.5
77	458.0
78	315.5
79	227.0
80	158.0
81	128.0
82	103.5
83	81.0
84	63.0
85	54.5
86	58.0
87	49.0
88	43.0
89	44.0
90	37.0
91	27.0
92	28.0
93	23.0
94	15.0
95	10.5
96	8.5
97	10.5
98	9.0
99	7.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05457443004716908
2	0.07359129133402291
3	0.07141227597823757
4	0.06949738369588077
5	0.06537046067356006
6	0.010432861400426756
7	0.014031538275890418
8	0.01805941514567543
9	0.00696624606167736
10-11	0.013090599826801294
12-13	0.02283013815947817
14-15	0.04017972254531444
16-17	0.02784847655462016
18-19	0.009855092177301857
20-21	0.020287953577728612
22-23	0.03748896873476134
24-25	0.03369219955422628
26-27	0.020799692032496382
28-29	0.011489353694140858
30-31	0.011769984459658666
32-33	0.008633522962694927
34-35	0.005249446084391944
36-37	6.27292299392748E-4
38-39	0.003994861485606448
40-41	0.0012380769066962132
42-43	0.22940739696476367
44-45	0.1990002281363047
46-47	0.20481093575173223
48-49	0.2122724125760881
50-51	0.2040515819156252
52-53	0.2109187818247669
54-55	0.20751819725437462
56-57	0.20994482799149922
58-59	0.21877644325926554
60-61	0.22272178166860412
62-63	0.2088553203136065
64-65	0.20921848953957076
66-67	0.20621408957932127
68-69	0.20164145887058993
70-71	0.1936022128231092
72-73	0.20509156651725002
74-75	0.2042826896048752
76-77	0.19786119738214414
78-79	0.21516125869171257
80-81	0.2261058585469071
82-83	0.2249503201006573
84-85	0.2192881817140333
86-87	0.23911391991326197
88-89	0.23125625847876335
90-91	0.23799139685119075
92-93	0.233220673837388
94-95	0.23676982763658383
96-97	0.24373607369826117
98-99	0.2366707814840481
100-101	0.2436040121615469
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3028891.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.3419431000882
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.34310856669022	44.35004130615095
2	12.268739653267275	14.070267422148957
3	4.218990411248645	7.257753243049125
4	1.9477694263205534	4.467555344646584
5	1.0642635265703855	3.051346929204913
6	0.6709464652354077	2.3084024419640436
7	0.4675646965196971	1.8767747756409734
8	0.3204891019351437	1.4701974277890701
9	0.24986327226076957	1.2894880986701007
>10	1.3555444716984733	13.81079577540349
>50	0.06710780232672652	2.5923328836647483
>100	0.023478401923820613	2.2593314189945213
>500	0.0012113049751012906	0.48250560895027717
>1k	9.228990277830337E-4	0.7132073237222072
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.3015384178565684E-5
2	0.0	0.0	0.0	0.0	3.3015384178565684E-5
3	0.0	0.0	0.0	0.0	3.3015384178565684E-5
4	0.0	0.0	0.0	0.0	3.3015384178565684E-5
5	0.0	0.0	0.0	0.0	3.3015384178565684E-5
6	0.0	0.0	0.0	0.0	3.3015384178565684E-5
7	0.0	0.0	0.0	0.0	3.3015384178565684E-5
8	0.0	0.0	0.0	0.0	6.603076835713137E-5
9	0.0	0.0	0.0	0.0	6.603076835713137E-5
10-11	0.0	0.0	0.0	3.3015384178565684E-5	6.603076835713137E-5
12-13	0.0	0.0	0.0	3.3015384178565684E-5	9.904615253569706E-5
14-15	0.0	0.0	0.0	3.3015384178565684E-5	2.1459999716067694E-4
16-17	0.0	0.0	0.0	4.952307626784853E-5	2.6412307342852547E-4
18-19	0.0	0.0	0.0	9.904615253569706E-5	2.971384576070912E-4
20-21	0.0	0.0	0.0	1.485692288035456E-4	3.6316922596422253E-4
22-23	0.0	0.0	0.0	2.8063076551780835E-4	3.9618461014278824E-4
24-25	0.0	0.0	0.0	5.777692231248995E-4	4.2919999432135394E-4
26-27	0.0	0.0	0.0	0.001039984601624819	4.4570768641063677E-4
28-29	0.0	0.0	0.0	0.0038132768726243365	4.7872307058920247E-4
30-31	0.0	0.0	0.0	0.01247981521949783	4.952307626784853E-4
32-33	0.0	0.0	0.0	0.03780261488445771	4.952307626784853E-4
34-35	0.0	0.0	0.0	0.0728319374979159	5.612615310356167E-4
36-37	0.0	1.6507692089282842E-5	0.0	0.13387738284408385	5.612615310356167E-4
38-39	0.0	3.3015384178565684E-5	0.0	0.23675331994449453	5.612615310356167E-4
40-41	0.0	3.3015384178565684E-5	0.0	0.35238970302992084	5.612615310356167E-4
42-43	0.0	3.3015384178565684E-5	0.0	0.4712615937648466	5.612615310356167E-4
44-45	0.0	3.3015384178565684E-5	0.0	0.6229837917574452	5.612615310356167E-4
46-47	0.0	3.3015384178565684E-5	0.0	0.8011182970928964	5.612615310356167E-4
48-49	0.0	3.3015384178565684E-5	0.0	0.9827854485354541	5.942769152141824E-4
50-51	0.0	3.3015384178565684E-5	0.0	1.1783685844092773	6.603076835713138E-4
52-53	0.0	3.3015384178565684E-5	0.0	1.3796138586697244	6.933230677498794E-4
54-55	0.0	3.3015384178565684E-5	0.0	1.6090872864028452	7.263384519284451E-4
56-57	0.0	3.3015384178565684E-5	0.0	1.8724014829189959	7.263384519284451E-4
58-59	0.0	3.3015384178565684E-5	0.0	2.15151354076459	7.263384519284451E-4
60-61	0.0	3.3015384178565684E-5	0.0	2.447001229162753	7.758615281962936E-4
62-63	0.0	3.3015384178565684E-5	0.0	2.7527567020404495	8.253846044641421E-4
64-65	0.0	3.3015384178565684E-5	0.0	3.082646420752678	8.253846044641421E-4
66-67	0.0	3.3015384178565684E-5	0.0	3.4559678773518097	8.41892296553425E-4
68-69	0.0	3.3015384178565684E-5	0.0	3.860043164313275	8.583999886427079E-4
70-71	0.0	3.3015384178565684E-5	0.0	4.285000681767683	8.749076807319907E-4
72-73	0.0	3.3015384178565684E-5	0.0	4.727852537446874	9.904615253569706E-4
74-75	0.0	3.3015384178565684E-5	0.0	5.18739366982833	9.904615253569706E-4
76-77	0.0	3.3015384178565684E-5	0.0	5.698818478446402	9.904615253569706E-4
78-79	0.0	3.3015384178565684E-5	0.0	6.2612190402361785	0.0010069692174462534
80-81	0.0	3.3015384178565684E-5	0.0	6.850708724744469	0.0010564922937141019
82-83	0.0	3.3015384178565684E-5	0.0	7.449970962969616	0.0010895076778926675
84-85	0.0	3.3015384178565684E-5	0.0	8.068728785552203	0.0010895076778926675
86-87	0.0	3.3015384178565684E-5	0.0	8.72528922301925	0.0011225230620712334
88-89	0.0	3.3015384178565684E-5	0.0	9.444347782736322	0.0011225230620712334
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2830	0.0	57.696617	1
GTATCAA	5730	0.0	44.317726	1
TCAACGC	7005	0.0	35.71457	4
CAACGCA	7155	0.0	34.829685	5
ATCAACG	7275	0.0	34.51902	3
TATCAAC	7530	0.0	33.414196	2
AACGCAG	7565	0.0	33.235435	6
GTACATG	11350	0.0	30.110294	1
TACATGG	11835	0.0	28.399788	2
ACGCAGA	8850	0.0	27.772448	7
CGCAGAG	9075	0.0	26.823334	8
ACATGGG	12305	0.0	26.542545	3
CATGGGG	9725	0.0	24.163425	4
GCAGAGT	10140	0.0	22.977728	9
ATGGGGG	5995	0.0	20.982468	5
AGAGTAC	8825	0.0	19.680218	10-11
CAGAGTA	9540	0.0	19.349277	10-11
ACGTATA	230	2.0711923E-6	18.576538	2
GAGTACA	8530	0.0	18.084757	1
GTATAGA	895	0.0	18.031597	1
>>END_MODULE