FastQCFastQC Report
Mon 15 Aug 2016
SRR2921691_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921691_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3028891
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA25500.057.423351
GTATCAA53950.046.176161
TCAACGC67600.035.454894
ATCAACG70850.033.8966263
CAACGCA71300.033.7743575
TATCAAC71550.033.7640042
AACGCAG76750.031.37196
GTACATG112850.029.827011
TACATGG117200.028.3880942
ACATGGG118800.026.7672753
ACGCAGA90050.026.580197
CGCAGAG93800.025.3150318
CATGGGG92850.025.0975344
ATGGGGG57100.023.2079495
GCAGAGT104150.021.6600749
AGAGTAC91700.019.2663410-11
GTATATA10250.018.0891741
GAGTACA85450.018.0264781
GTATAGG8250.017.2880211
CAGAGTA98200.017.1930810-11
Produced by FastQC (version 0.11.5)