##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921691_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3028891
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36281959304577	33.0	31.0	34.0	28.0	34.0
2	31.502452877967546	34.0	31.0	34.0	28.0	34.0
3	31.57387340779183	34.0	31.0	34.0	28.0	34.0
4	35.05242380792178	37.0	35.0	37.0	32.0	37.0
5	34.8017875189302	37.0	35.0	37.0	32.0	37.0
6	34.773387685459795	37.0	35.0	37.0	32.0	37.0
7	34.662869017075884	37.0	35.0	37.0	32.0	37.0
8	34.60581116983081	37.0	35.0	37.0	32.0	37.0
9	36.185942973847524	39.0	37.0	39.0	32.0	39.0
10-11	36.08193824076205	39.0	37.0	39.0	32.0	39.0
12-13	36.002483747351754	39.0	37.0	39.0	31.0	39.0
14-15	37.22776933867874	40.0	38.0	41.0	31.0	41.0
16-17	37.19661965386011	40.0	38.0	41.0	31.0	41.0
18-19	37.165044895970176	40.0	38.0	41.0	31.0	41.0
20-21	37.1087376534844	40.0	38.0	41.0	31.0	41.0
22-23	37.0243970482926	40.0	37.5	41.0	31.0	41.0
24-25	36.93115945737235	40.0	37.0	41.0	30.5	41.0
26-27	36.80137779801254	40.0	37.0	41.0	30.0	41.0
28-29	36.683982850488846	40.0	37.0	41.0	30.0	41.0
30-31	36.517774162226374	40.0	37.0	41.0	30.0	41.0
32-33	36.44828915929956	40.0	36.5	41.0	30.0	41.0
34-35	36.560913879040214	40.0	37.0	41.0	30.0	41.0
36-37	36.55009341702953	40.0	37.0	41.0	30.0	41.0
38-39	36.43745450067368	40.0	37.0	41.0	30.0	41.0
40-41	36.35255560533542	40.0	36.5	41.0	29.5	41.0
42-43	36.21927514063728	40.0	36.0	41.0	29.0	41.0
44-45	36.083061919362564	40.0	36.0	41.0	28.0	41.0
46-47	35.915233331275374	40.0	36.0	41.0	28.0	41.0
48-49	35.76580174063709	40.0	35.0	41.0	27.0	41.0
50-51	35.609394494552625	39.5	35.0	41.0	27.0	41.0
52-53	35.43683397652804	39.0	35.0	41.0	26.5	41.0
54-55	35.220543096466656	39.0	35.0	41.0	26.0	41.0
56-57	34.99434248376716	39.0	35.0	41.0	26.0	41.0
58-59	34.75330921449468	38.5	35.0	40.5	26.0	41.0
60-61	34.47561335155342	38.0	34.0	40.0	25.0	41.0
62-63	34.17418933200304	37.5	34.0	40.0	24.0	41.0
64-65	33.84249697331465	37.0	34.0	40.0	23.0	41.0
66-67	33.49671381373578	36.0	34.0	39.0	22.5	41.0
68-69	33.135753482050035	36.0	33.5	39.0	22.0	41.0
70-71	32.73750491516532	35.0	33.0	39.0	21.5	40.0
72-73	32.38069725850154	35.0	33.0	37.5	20.0	40.0
74-75	31.976793816614727	35.0	33.0	37.0	19.5	39.0
76-77	29.279446668764244	33.0	29.0	34.5	12.0	36.5
78-79	30.840881695643716	34.5	31.5	36.0	14.0	37.5
80-81	31.015296522720693	35.0	32.0	35.5	15.5	37.0
82-83	30.838898461516113	35.0	32.0	35.0	12.5	37.0
84-85	30.63694170572662	35.0	32.0	35.0	9.5	36.5
86-87	30.427694988033572	35.0	32.0	35.0	7.0	36.0
88-89	30.155263923330352	35.0	31.5	35.0	2.0	36.0
90-91	29.950882847880628	34.5	31.0	35.0	2.0	35.0
92-93	29.786306275135026	34.0	31.0	35.0	2.0	35.0
94-95	29.67485393168655	34.0	31.0	35.0	2.0	35.0
96-97	29.47022309485551	34.0	31.0	35.0	2.0	35.0
98-99	29.2646174788066	34.0	31.0	35.0	2.0	35.0
100-101	28.150473556162964	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	57360.0
3	19648.0
4	10813.0
5	7685.0
6	6618.0
7	6795.0
8	6899.0
9	7521.0
10	8302.0
11	9056.0
12	9741.0
13	10486.0
14	11348.0
15	12287.0
16	13402.0
17	14587.0
18	13720.0
19	12229.0
20	11510.0
21	11517.0
22	12000.0
23	13461.0
24	15895.0
25	18941.0
26	22604.0
27	27143.0
28	32578.0
29	39680.0
30	48016.0
31	59087.0
32	75595.0
33	99919.0
34	144508.0
35	213879.0
36	366876.0
37	710405.0
38	765209.0
39	101566.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.117584731750036	19.730638884406076	13.534360970086274	25.617415413757616
2	15.064954616948024	21.35743726072045	37.56043480040834	26.01717332192319
3	17.57539616894976	25.546949731044595	29.442650968163512	27.435003131842134
4	12.926498852148166	17.115786602903356	36.08520616594375	33.87250837900473
5	13.846985534316206	38.065414585625724	34.197452422813804	13.890147457244268
6	32.65759452568035	36.05547086947349	17.39584189686259	13.89109270798357
7	28.43905574680634	32.393506402178225	21.334640302341683	17.832797548673756
8	27.04293419604733	34.47889012843315	20.122282379920573	18.35589329559895
9	27.247680585191468	15.221356741696379	19.433066051611664	38.097896621500496
10-11	25.538992376871285	25.55816704688446	27.942845279901462	20.95999529634279
12-13	26.11801768741644	22.96272231634403	27.761382809764612	23.157877186474916
14-15	23.40385033178967	24.4264585175417	25.116187466281204	27.05350368438743
16-17	23.454628023033518	27.454260891900844	25.855886833809276	23.23522425125636
18-19	23.523180509028432	26.929607076748802	27.13118533330406	22.416027080918706
20-21	24.106737680732852	26.02241056811858	26.991992263816023	22.87885948733254
22-23	23.968723205952276	25.78016178198555	26.746208430742474	23.5049065813197
24-25	23.693174680446695	26.00990829437912	26.691943365799165	23.60497365937502
26-27	23.410313829168178	26.28957531825749	26.721974645053027	23.578136207521304
28-29	23.518154808531868	26.55851961004591	26.484560864168326	23.4387647172539
30-31	23.99311378955881	26.151419360441636	26.32017345298842	23.53529339701113
32-33	23.762723270153664	26.26412164942054	26.14820640936488	23.824948671060913
34-35	24.023756598871802	26.35806003404932	26.383319663416266	23.234863703662615
36-37	24.110604467543034	26.069741823088123	26.455873887517868	23.36377982185098
38-39	23.96577624146139	26.25711069071508	26.22320387996923	23.5539091878543
40-41	24.067018314940913	26.07562969165183	26.278483378147797	23.578868615259456
42-43	23.884878285222598	26.03974129625264	26.13901034062176	23.936370077903003
44-45	23.70980778526468	26.035693248843728	26.385216608071595	23.869282357819998
46-47	23.83644253095097	26.050005241379992	26.115592651502705	23.99795957616633
48-49	23.58624975195047	26.191653054897117	26.208459339192753	24.01363785395966
50-51	23.815533575090207	26.157896500611383	26.185927399312035	23.84064252498638
52-53	23.98551256961222	26.005531834460964	26.19537396009849	23.813581635828328
54-55	23.35992213370294	25.90005254588355	26.53900586754112	24.201019452872387
56-57	23.514290591483856	25.982980653890763	26.305498352247568	24.19723040237781
58-59	23.585768547377583	25.893023718097098	26.380781965145832	24.140425769379487
60-61	23.70168510398323	26.027793245617616	26.407826101455566	23.862695548943595
62-63	23.610692141349087	25.909915639716054	26.468024640540456	24.011367578394402
64-65	23.240774396322987	25.743371686751665	26.483163893608776	24.532690023316576
66-67	23.353394894509975	25.987160292512495	26.232979937998348	24.426464874979185
68-69	23.44729070401776	25.847941703383377	26.279772163434927	24.424995429163932
70-71	23.361069124728466	25.73627045287476	26.22106036953523	24.681600052861544
72-73	23.32121919489859	26.041764714236127	26.037553829147715	24.599462261717573
74-75	23.25640339753243	26.12804153126734	25.9761466011466	24.63940847005363
76-77	23.268325943760935	25.673950677685863	26.16912951736112	24.888593861192078
78-79	23.198047120245846	25.8241747348247	26.180930509202653	24.796847635726795
80-81	23.414856306900766	26.04575667066592	25.861090641302713	24.6782963811306
82-83	23.315060920152888	25.99733757102728	26.003810357912087	24.68379115090774
84-85	23.21369968039298	26.096587681842454	25.885525820517646	24.804186817246922
86-87	23.075474493864125	26.262190763572658	25.80967120557296	24.85266353699026
88-89	22.996681590101627	25.932504969833314	25.908448443453334	25.162364996611718
90-91	22.992039886005923	25.918701400023707	26.01215111169393	25.07710760227644
92-93	23.18670219926055	25.752927351150834	25.98229261206682	25.07807783752179
94-95	22.945649254262353	25.727911625866724	26.000663347507754	25.325775772363173
96-97	22.729529379929037	25.621681540486712	26.100578205892543	25.54821087369171
98-99	22.50911026829379	25.637000689202626	26.010243119322844	25.84364592318073
100-101	22.534293113512284	25.723252229861256	25.993087232013863	25.749367424612597
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	75.0
1	68.5
2	102.5
3	150.0
4	134.0
5	149.5
6	242.5
7	468.0
8	689.5
9	704.5
10	598.5
11	489.0
12	475.5
13	531.0
14	585.0
15	640.5
16	684.5
17	753.5
18	852.0
19	964.5
20	1100.0
21	1224.0
22	1385.5
23	1658.5
24	2042.0
25	2633.5
26	3596.5
27	5070.5
28	7279.0
29	10680.0
30	15420.0
31	21403.5
32	29552.0
33	35815.5
34	40818.0
35	47595.0
36	56024.0
37	64934.0
38	72659.5
39	80312.0
40	85856.5
41	90186.5
42	95485.5
43	101470.0
44	107645.5
45	114918.5
46	120481.5
47	124918.0
48	130327.0
49	132746.0
50	133247.5
51	132373.0
52	129844.5
53	128144.0
54	125327.0
55	119531.5
56	110666.0
57	98548.5
58	87459.0
59	76057.5
60	62610.5
61	51568.0
62	42572.5
63	32546.0
64	22888.0
65	16360.5
66	11971.0
67	8907.5
68	6656.5
69	4830.0
70	3474.5
71	2507.0
72	1837.0
73	1384.0
74	1049.0
75	746.5
76	494.0
77	291.0
78	165.0
79	86.5
80	59.5
81	42.5
82	23.5
83	18.0
84	12.5
85	7.5
86	5.0
87	5.0
88	3.5
89	2.5
90	2.0
91	1.0
92	1.5
93	2.0
94	1.0
95	0.0
96	1.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1649118439719356
2	2.311076892499598E-4
3	0.010630953705498151
4	0.0062729229939274805
5	0.025058676591531357
6	0.0019148922823568097
7	0.0
8	0.0
9	6.603076835713137E-5
10-11	0.04818595320861662
12-13	4.787230705892024E-4
14-15	0.005447538389463338
16-17	1.6507692089282842E-5
18-19	0.0014031538275890416
20-21	2.4761538133924265E-4
22-23	0.0
24-25	4.787230705892024E-4
26-27	0.0030869384206958918
28-29	0.005794199923338278
30-31	0.017167999772854155
32-33	0.012710922908747788
34-35	0.011324276773248031
36-37	0.004440569172017085
38-39	3.3015384178565684E-5
40-41	0.0035326461071065285
42-43	0.008352892197177119
44-45	0.006437999914820309
46-47	0.003582169183374377
48-49	0.008650030654784209
50-51	0.002987892268160195
52-53	0.0019313999744460925
54-55	0.0036151845675529426
56-57	0.007263384519284451
58-59	0.014658830575283164
60-61	0.006784661448695248
62-63	0.020783184340407098
64-65	0.04016321485322516
66-67	0.06190384533481066
68-69	0.05102527624797327
70-71	0.069860552921845
72-73	0.03380775339885126
74-75	0.02677547656881677
76-77	0.22953945850147794
78-79	0.0855428604066637
80-81	0.06852342986261309
82-83	0.027369753484030958
84-85	0.0521477993100445
86-87	0.0680281990999346
88-89	0.2945137345648952
90-91	0.29994476526226926
92-93	0.05360047621390139
94-95	0.010317307555801777
96-97	0.01556675364019372
98-99	9.40938449089122E-4
100-101	9.904615253569706E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3028891.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.41941383918972
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.53536176254788	43.180802486132855
2	12.698584898512616	14.328934331225366
3	4.308286480865564	7.292129937052218
4	2.0475356746025626	4.6208305030362595
5	1.133972019850171	3.1989018334994332
6	0.7037841541436914	2.382425366765821
7	0.4718862526795763	1.8636482042467424
8	0.33763122913510407	1.5239164833286183
9	0.26564044853362	1.348855055842166
>10	1.4030257298136775	14.168174114680298
>50	0.06804263891648338	2.589397682677377
>100	0.024134310551590047	2.3298951745692027
>500	0.0011159332532589352	0.42617495714101106
>1k	9.984665942410567E-4	0.7459138698026072
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.3015384178565684E-5	0.0
4	0.0	0.0	0.0	3.3015384178565684E-5	0.0
5	0.0	0.0	0.0	3.3015384178565684E-5	0.0
6	0.0	0.0	0.0	3.3015384178565684E-5	0.0
7	0.0	0.0	0.0	3.3015384178565684E-5	0.0
8	0.0	0.0	0.0	3.3015384178565684E-5	0.0
9	0.0	0.0	0.0	3.3015384178565684E-5	0.0
10-11	0.0	0.0	0.0	6.603076835713137E-5	3.3015384178565684E-5
12-13	0.0	0.0	0.0	6.603076835713137E-5	3.3015384178565684E-5
14-15	0.0	0.0	0.0	6.603076835713137E-5	4.952307626784853E-5
16-17	0.0	0.0	0.0	8.25384604464142E-5	1.3206153671426274E-4
18-19	0.0	0.0	0.0	9.904615253569706E-5	1.6507692089282844E-4
20-21	0.0	0.0	0.0	1.485692288035456E-4	1.6507692089282844E-4
22-23	0.0	0.0	0.0	2.971384576070912E-4	1.815846129821113E-4
24-25	0.0	0.0	0.0	6.437999914820308E-4	2.311076892499598E-4
26-27	0.0	0.0	0.0	0.0011225230620712334	2.311076892499598E-4
28-29	0.0	0.0	0.0	0.004027876869785014	2.311076892499598E-4
30-31	0.0	0.0	0.0	0.013371230592319101	2.311076892499598E-4
32-33	0.0	0.0	0.0	0.03953592255383241	2.311076892499598E-4
34-35	0.0	0.0	0.0	0.07517602977459406	2.311076892499598E-4
36-37	0.0	0.0	0.0	0.13721193664611897	2.311076892499598E-4
38-39	0.0	0.0	0.0	0.2402694583595118	2.6412307342852547E-4
40-41	0.0	0.0	0.0	0.3566651952810451	2.6412307342852547E-4
42-43	0.0	0.0	0.0	0.47708880907236345	2.6412307342852547E-4
44-45	0.0	0.0	0.0	0.629157668598837	2.6412307342852547E-4
46-47	0.0	0.0	0.0	0.8082991431517343	2.6412307342852547E-4
48-49	0.0	0.0	0.0	0.9901313715151849	2.971384576070912E-4
50-51	0.0	0.0	0.0	1.1863087843042224	2.971384576070912E-4
52-53	0.0	0.0	0.0	1.3879667508669016	3.301538417856568E-4
54-55	0.0	0.0	0.0	1.6173246247553972	3.6316922596422253E-4
56-57	0.0	0.0	0.0	1.8810350058816907	3.6316922596422253E-4
58-59	0.0	0.0	0.0	2.1610549867921955	3.6316922596422253E-4
60-61	0.0	0.0	0.0	2.4570709213372153	3.6316922596422253E-4
62-63	0.0	0.0	0.0	2.763899394200716	3.9618461014278824E-4
64-65	0.0	0.0	0.0	3.09548940519814	3.9618461014278824E-4
66-67	0.0	0.0	0.0	3.4696032310175573	3.9618461014278824E-4
68-69	0.0	0.0	0.0	3.8746854871964693	3.9618461014278824E-4
70-71	0.0	0.0	0.0	4.3021851892326275	4.2919999432135394E-4
72-73	0.0	1.6507692089282842E-5	0.0	4.747661767954013	4.622153784999196E-4
74-75	0.0	3.3015384178565684E-5	0.0	5.211181254128986	4.7872307058920247E-4
76-77	0.0	3.3015384178565684E-5	0.0	5.725891093472826	4.952307626784853E-4
78-79	0.0	3.3015384178565684E-5	0.0	6.292319532132388	4.952307626784853E-4
80-81	0.0	3.3015384178565684E-5	0.0	6.886332324273141	5.117384547677681E-4
82-83	0.0	3.3015384178565684E-5	0.0	7.491702408571323	5.777692231248995E-4
84-85	0.0	3.3015384178565684E-5	0.0	8.115280477243981	5.942769152141824E-4
86-87	0.0	3.3015384178565684E-5	0.0	8.778031299244509	6.27292299392748E-4
88-89	0.0	3.3015384178565684E-5	0.0	9.504122135791615	6.603076835713138E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2550	0.0	57.42335	1
GTATCAA	5395	0.0	46.17616	1
TCAACGC	6760	0.0	35.45489	4
ATCAACG	7085	0.0	33.896626	3
CAACGCA	7130	0.0	33.774357	5
TATCAAC	7155	0.0	33.764004	2
AACGCAG	7675	0.0	31.3719	6
GTACATG	11285	0.0	29.82701	1
TACATGG	11720	0.0	28.388094	2
ACATGGG	11880	0.0	26.767275	3
ACGCAGA	9005	0.0	26.58019	7
CGCAGAG	9380	0.0	25.315031	8
CATGGGG	9285	0.0	25.097534	4
ATGGGGG	5710	0.0	23.207949	5
GCAGAGT	10415	0.0	21.660074	9
AGAGTAC	9170	0.0	19.26634	10-11
GTATATA	1025	0.0	18.089174	1
GAGTACA	8545	0.0	18.026478	1
GTATAGG	825	0.0	17.288021	1
CAGAGTA	9820	0.0	17.19308	10-11
>>END_MODULE