FastQCFastQC Report
Mon 15 Aug 2016
SRR2921690_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921690_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2676310
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA34300.044.5543561
GGTATCA17850.044.136541
TCAACGC40850.036.5998574
CAACGCA42250.035.3850985
ATCAACG43950.033.9096683
TATCAAC44750.033.728342
AACGCAG44700.033.5323836
GTACATG75200.028.7158681
ACGCAGA53550.028.0833957
TACATGG78850.026.9672832
CGCAGAG57850.025.6717648
ACATGGG82150.025.1902053
GTATATA7700.024.038221
TATCGCG601.7012226E-423.7720490-91
CATGGGG71850.022.1959324
GCAGAGT68250.020.786599
GAGTACA57500.020.5522751
AGTACAT56900.019.4358942
AGAGTAC55400.019.09914210-11
ATGGGGG42450.018.2241155
Produced by FastQC (version 0.11.5)