##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921690_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2676310
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.21318046115734	31.0	31.0	34.0	26.0	34.0
2	30.268413972970247	31.0	31.0	34.0	26.0	34.0
3	30.3270775806988	33.0	31.0	34.0	26.0	34.0
4	33.76594938553456	37.0	35.0	37.0	28.0	37.0
5	33.758321345434574	37.0	35.0	37.0	28.0	37.0
6	33.798969476630134	37.0	35.0	37.0	29.0	37.0
7	33.704000657621876	37.0	35.0	37.0	28.0	37.0
8	33.67476562879487	37.0	35.0	37.0	28.0	37.0
9	35.21632807858581	39.0	35.0	39.0	28.0	39.0
10-11	35.10996726836577	39.0	35.0	39.0	28.0	39.0
12-13	34.998725297144205	39.0	35.0	39.0	27.0	39.0
14-15	36.131021817353	40.0	36.0	41.0	27.0	41.0
16-17	36.06991865665786	40.0	36.0	41.0	27.0	41.0
18-19	36.026271807077656	40.0	36.0	41.0	27.0	41.0
20-21	35.949580018757175	40.0	36.0	41.0	27.0	41.0
22-23	35.85143574548539	40.0	36.0	41.0	27.0	41.0
24-25	35.74533181881023	40.0	36.0	41.0	27.0	41.0
26-27	35.60192447810605	40.0	36.0	41.0	26.0	41.0
28-29	35.462601118704484	40.0	36.0	41.0	25.0	41.0
30-31	35.321219141280345	40.0	35.5	41.0	25.0	41.0
32-33	35.14959515153327	39.0	35.0	41.0	24.0	41.0
34-35	34.97958009348693	39.0	35.0	41.0	24.0	41.0
36-37	34.95310315322216	39.0	35.0	41.0	24.0	41.0
38-39	35.13737982520709	39.5	35.0	41.0	24.0	41.0
40-41	35.19720379926092	40.0	35.0	41.0	24.0	41.0
42-43	35.04383199255692	40.0	35.0	41.0	23.0	41.0
44-45	34.92896170473525	40.0	35.0	41.0	22.5	41.0
46-47	34.77378125105089	40.0	35.0	41.0	21.5	41.0
48-49	34.61259215113347	39.0	35.0	41.0	19.5	41.0
50-51	32.933476503095676	37.0	32.5	39.5	16.0	40.0
52-53	33.04317623892598	37.5	32.5	39.5	14.5	40.0
54-55	33.94894406851224	39.0	34.0	40.5	15.0	41.0
56-57	33.964549510333256	39.0	34.0	41.0	14.5	41.0
58-59	33.77350250905164	38.0	34.0	41.0	13.0	41.0
60-61	33.51983458567953	38.0	34.0	40.0	12.0	41.0
62-63	33.22580661433092	37.0	33.0	40.0	8.0	41.0
64-65	32.93462696772795	37.0	33.0	40.0	7.5	41.0
66-67	32.59308133213268	36.0	33.0	39.0	4.5	41.0
68-69	32.23863715339404	36.0	33.0	39.0	2.0	41.0
70-71	31.837381132977868	35.0	32.0	39.0	2.0	40.5
72-73	31.484512257548637	35.0	32.0	37.5	2.0	40.0
74-75	31.097682256539787	35.0	32.0	37.0	2.0	39.0
76-77	30.739190340431414	35.0	31.5	36.5	2.0	39.0
78-79	30.411883152549592	35.0	31.0	36.0	2.0	38.5
80-81	30.117748504470708	35.0	31.0	35.5	2.0	37.0
82-83	29.824037760946975	35.0	31.0	35.0	2.0	37.0
84-85	29.58155464053118	35.0	31.0	35.0	2.0	36.0
86-87	29.365986750413818	35.0	31.0	35.0	2.0	36.0
88-89	29.16508831189212	35.0	30.0	35.0	2.0	36.0
90-91	28.965346503207776	34.0	30.0	35.0	2.0	35.5
92-93	28.791484917666487	34.0	30.0	35.0	2.0	35.0
94-95	28.582335379683222	34.0	30.0	35.0	2.0	35.0
96-97	28.36337569265145	34.0	30.0	35.0	2.0	35.0
98-99	28.11976919714084	34.0	29.5	35.0	2.0	35.0
100-101	27.073670651008293	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	104574.0
3	17361.0
4	11701.0
5	8738.0
6	7661.0
7	7853.0
8	8435.0
9	8954.0
10	9774.0
11	10230.0
12	10005.0
13	10365.0
14	10755.0
15	11479.0
16	11235.0
17	11649.0
18	11306.0
19	10608.0
20	10899.0
21	11351.0
22	12337.0
23	14159.0
24	16739.0
25	19725.0
26	23500.0
27	28335.0
28	32969.0
29	39281.0
30	47284.0
31	57292.0
32	71476.0
33	93146.0
34	128840.0
35	188405.0
36	316407.0
37	576927.0
38	600968.0
39	103579.0
40	8.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.65954521290714	17.412246039323765	12.669565692008963	25.258643055760132
2	15.918317287971831	19.007312676465013	39.58608239539604	25.488287640167112
3	19.511882318717895	23.20348120268703	28.76273426066694	28.521902217928137
4	13.471824379424438	15.15188112288946	36.07539821566181	35.3008962820243
5	14.609679715728266	36.760980234978824	34.128311119619056	14.501028929673854
6	34.3032571863327	34.646262479204445	16.804942504854164	14.245537829608693
7	29.707222264366557	31.141346246191592	20.544516438595224	18.60691505084662
8	26.89138470404061	35.02814238578519	19.34703536100771	18.73343754916649
9	26.872161261815037	15.741523721621961	18.890717804902202	38.4955972116608
10-11	26.039941434277242	24.94652402420358	27.42615008849134	21.587384453027838
12-13	26.512048833293377	22.160296937398613	27.41240613959145	23.915248089716563
14-15	23.544072187106348	23.740575717591124	24.42517951459105	28.29017258071148
16-17	24.048531881797068	26.995476158262697	25.157833638934342	23.79815832100589
18-19	24.107593450238817	26.354068787925726	26.343082390692352	23.195255371143105
20-21	24.571704165403794	25.54504688167446	26.288307068707837	23.594941884213906
22-23	24.398888363513354	25.310026594800277	26.049223742073423	24.241861299612946
24-25	24.17489282016588	25.61242949678741	25.981569180050908	24.231108502995802
26-27	24.03385284986641	25.785076895996163	26.042518061243975	24.13855219289345
28-29	24.263693063128038	25.905066909816554	25.863325348749434	23.967914678305974
30-31	24.39261139218108	25.689722791742625	25.7182356624263	24.199430153649992
32-33	24.098001155051783	25.811801295967353	25.767351794356692	24.32284575462418
34-35	24.409855147197575	25.746095625643413	25.79865217578139	24.045397051377623
36-37	24.47532719771833	25.587222147699407	25.827797990275332	24.109652664306928
38-39	24.315263963339	25.732294221404977	25.833146345250192	24.119295470005834
40-41	24.419229869587344	25.63872383769375	25.817124853434294	24.124921439284616
42-43	24.25818137833695	25.549709908353286	25.791399895819406	24.400708817490358
44-45	24.216890315134414	25.630266021536812	25.91248069449106	24.24036296883771
46-47	24.390973041060544	25.64591329194002	25.697410019717015	24.265703647282418
48-49	24.216521656433954	25.703279035930937	25.677407083747994	24.40279222388711
50-51	24.242824716154036	25.80058470752831	25.775164268821698	24.18142630749596
52-53	24.429026709568912	25.671352266932445	25.7568484970111	24.142772526487544
54-55	24.087274235740175	25.480338137681898	26.046365701316702	24.386021925261225
56-57	24.11973887570987	25.67632329331704	25.882601148970842	24.321336682002254
58-59	24.241039383912362	25.556051895805492	25.91543884861377	24.287469871668378
60-61	24.245996343419044	25.559856620087228	25.97417551401362	24.21997152248011
62-63	24.198071061219594	25.617490852039378	26.034514679324833	24.1499234074162
64-65	24.054967185697613	25.444384914818347	26.035693814048166	24.46495408543587
66-67	24.052142793095424	25.61969501067292	25.904811716460223	24.42335047977143
68-69	24.164908368848703	25.48911627377633	26.001836666237388	24.34413869113758
70-71	24.144445166085475	25.306503510076535	25.99244098697284	24.556610336865152
72-73	24.079185791408655	25.500456747508483	25.888673410556105	24.531684050526756
74-75	23.912104269668305	25.61231923937712	25.989302430408145	24.48627406054643
76-77	24.068960986235094	25.296551295564118	26.055447085717752	24.579040632483036
78-79	24.004016882288276	25.307968464029283	26.071711533334728	24.61630312034771
80-81	24.142118015891455	25.492200970743085	25.892623599545917	24.473057413819543
82-83	24.128273007595507	25.506424828227193	25.920941350752052	24.444360813425252
84-85	24.057279978012733	25.494034848995735	25.949768903405612	24.498916269585923
86-87	23.98041109950307	25.550886329959344	25.996490371930314	24.47221219860727
88-89	23.972747661969446	25.347816333951616	26.04573338681041	24.633702617268526
90-91	23.96987544352039	25.348692977153785	26.06656494816326	24.614866631162567
92-93	24.038767978219227	25.31327649710045	26.163647904967526	24.484307619712798
94-95	23.981311060805716	25.160670685064485	26.245596899483193	24.612421354646607
96-97	23.917421133305805	25.119342904736357	26.253566335478517	24.70966962647932
98-99	23.893360368191395	25.04798450114657	26.23369322801523	24.8249619026468
100-101	24.084619799864605	24.896229038640342	26.33586678364796	24.68328437784709
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	29.0
1	21.5
2	42.5
3	79.5
4	74.0
5	68.5
6	107.0
7	226.5
8	388.0
9	456.0
10	400.5
11	361.0
12	404.0
13	462.0
14	534.0
15	597.5
16	670.0
17	739.5
18	831.0
19	958.0
20	1011.5
21	1111.0
22	1249.0
23	1385.5
24	1610.5
25	1910.5
26	2370.5
27	3173.5
28	4497.0
29	6563.0
30	9660.0
31	13927.5
32	19848.0
33	25080.5
34	29468.0
35	34806.0
36	41901.5
37	49471.0
38	56181.0
39	62725.0
40	68700.0
41	74359.0
42	79834.0
43	86891.0
44	94516.5
45	101817.5
46	106892.0
47	110850.0
48	115650.0
49	118006.5
50	118114.5
51	118688.0
52	118778.5
53	117875.0
54	116705.0
55	112617.0
56	104730.0
57	95180.5
58	86862.5
59	76737.5
60	63077.5
61	51150.5
62	41817.5
63	32075.0
64	23252.5
65	16987.0
66	12389.5
67	9423.0
68	7195.0
69	5344.5
70	3971.5
71	2797.0
72	1968.5
73	1430.0
74	1101.0
75	797.5
76	547.0
77	362.5
78	263.0
79	206.0
80	158.0
81	120.5
82	97.0
83	86.0
84	77.5
85	63.0
86	50.5
87	47.5
88	45.0
89	36.0
90	34.0
91	33.5
92	30.0
93	24.0
94	11.5
95	6.0
96	8.5
97	10.0
98	8.0
99	7.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05186245240648505
2	0.07147901401556621
3	0.0684524587958794
4	0.06789198560704851
5	0.06299718642459207
6	0.009640138847891312
7	0.011994126240981052
8	0.016926290302692885
9	0.006165205077139794
10-11	0.0127787887053443
12-13	0.022213420717330953
14-15	0.038859474425608394
16-17	0.02645433451281802
18-19	0.0102192944763499
20-21	0.01984075088461352
22-23	0.036430757274007866
24-25	0.033628391329853416
26-27	0.020662778228232155
28-29	0.01195676136172566
30-31	0.01216226819763032
32-33	0.008948888581666549
34-35	0.00541790749203194
36-37	8.593922228740318E-4
38-39	0.004278278674742462
40-41	0.0013451356531941367
42-43	0.2222089369318203
44-45	0.1911774047102167
46-47	0.1967821365985256
48-49	0.20416170025146563
50-51	0.19545568338495914
52-53	0.2029660241152931
54-55	0.19904271179347682
56-57	0.20169561822060972
58-59	0.21114893267222407
60-61	0.21606241429430822
62-63	0.20025707036927712
64-65	0.2006867664807141
66-67	0.19797781273469817
68-69	0.1930269662333586
70-71	0.18419017228945825
72-73	0.19616561609081157
74-75	0.1956425077812361
76-77	0.1890849714719147
78-79	0.20857075600360198
80-81	0.21976153734059206
82-83	0.21856586120441954
84-85	0.2119896424554704
86-87	0.23265242068370257
88-89	0.22445082968714386
90-91	0.2315127918664131
92-93	0.22661799268395666
94-95	0.23070944696242215
96-97	0.23829451745126687
98-99	0.23080285916056062
100-101	0.23792086865871295
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2676310.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.81395520336112
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.92577220606512	49.40528102739754
2	11.130550863114905	13.760467448826343
3	3.6344389268645263	6.73977135043667
4	1.693180327340251	4.1864869162169045
5	0.9307635628671981	2.8767088589996885
6	0.5884394171156082	2.1824260661685675
7	0.4000318115506652	1.7309283935378554
8	0.28557040195283434	1.4121788826974664
9	0.21774495862009438	1.211370940611021
>10	1.1247072084257284	12.095390489848906
>50	0.051278824181096784	2.1217789650352428
>100	0.016249029859301867	1.7012995657708134
>500	0.001151275181491829	0.4954573333430947
>1k	1.2118686120047691E-4	0.08045376110983905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.7364879255392686E-5
2	0.0	0.0	0.0	3.7364879255392686E-5	3.7364879255392686E-5
3	0.0	0.0	0.0	3.7364879255392686E-5	3.7364879255392686E-5
4	0.0	0.0	0.0	3.7364879255392686E-5	3.7364879255392686E-5
5	0.0	0.0	0.0	3.7364879255392686E-5	3.7364879255392686E-5
6	0.0	0.0	0.0	3.7364879255392686E-5	7.472975851078537E-5
7	0.0	0.0	0.0	3.7364879255392686E-5	7.472975851078537E-5
8	0.0	0.0	0.0	3.7364879255392686E-5	1.1209463776617806E-4
9	0.0	0.0	0.0	3.7364879255392686E-5	1.1209463776617806E-4
10-11	0.0	0.0	0.0	9.341219813848172E-5	1.4945951702157075E-4
12-13	0.0	0.0	0.0	1.8682439627696343E-4	2.2418927553235612E-4
14-15	0.0	0.0	0.0	1.8682439627696343E-4	2.8023659441544515E-4
16-17	0.0	0.0	0.0	2.8023659441544515E-4	2.989190340431415E-4
18-19	0.0	0.0	0.0	4.4837855106471224E-4	2.989190340431415E-4
20-21	0.0	0.0	0.0	4.670609906924086E-4	2.989190340431415E-4
22-23	0.0	0.0	0.0	5.97838068086283E-4	3.362839132985342E-4
24-25	0.0	0.0	0.0	7.472975851078537E-4	3.7364879255392686E-4
26-27	0.0	0.0	0.0	8.780746625017281E-4	3.7364879255392686E-4
28-29	0.0	0.0	0.0	0.002372669832717436	3.7364879255392686E-4
30-31	0.0	0.0	0.0	0.008238955875814087	3.7364879255392686E-4
32-33	0.0	0.0	0.0	0.02155953533036158	3.923312321816232E-4
34-35	0.0	0.0	0.0	0.039233123218162316	4.4837855106471224E-4
36-37	0.0	0.0	0.0	0.06981627688870123	4.4837855106471224E-4
38-39	0.0	1.8682439627696343E-5	0.0	0.12388325717125445	4.857434303201049E-4
40-41	0.0	3.7364879255392686E-5	0.0	0.18895419439452082	4.857434303201049E-4
42-43	0.0	3.7364879255392686E-5	0.0	0.2524184418098053	5.044258699478013E-4
44-45	0.0	5.604731888308903E-5	0.0	0.3302494852987883	5.41790749203194E-4
46-47	0.0	7.472975851078537E-5	0.0	0.42016806722689076	6.165205077139793E-4
48-49	0.0	7.472975851078537E-5	0.0	0.5220807753959743	7.472975851078537E-4
50-51	0.0	7.472975851078537E-5	0.0	0.6347919336698664	7.472975851078537E-4
52-53	0.0	7.472975851078537E-5	0.0	0.7505296471634453	8.407097832463354E-4
54-55	0.0	7.472975851078537E-5	0.0	0.8766174322107678	8.967571021294245E-4
56-57	0.0	7.472975851078537E-5	0.0	1.0236295496411103	8.967571021294245E-4
58-59	0.0	7.472975851078537E-5	0.0	1.1865404231946224	9.341219813848172E-4
60-61	0.0	7.472975851078537E-5	0.0	1.3669006953604028	9.714868606402098E-4
62-63	0.0	7.472975851078537E-5	0.0	1.5535195848014618	9.714868606402098E-4
64-65	0.0	7.472975851078537E-5	0.0	1.7448464490287	9.714868606402098E-4
66-67	0.0	7.472975851078537E-5	0.0	1.9646453512485476	9.714868606402098E-4
68-69	0.0	7.472975851078537E-5	0.0	2.2171011579376083	9.714868606402098E-4
70-71	0.0	7.472975851078537E-5	0.0	2.4858480519820203	9.714868606402098E-4
72-73	1.8682439627696343E-5	7.472975851078537E-5	0.0	2.766645119586296	0.0010462166191509952
74-75	3.7364879255392686E-5	7.472975851078537E-5	0.0	3.05678340700442	0.0010462166191509952
76-77	3.7364879255392686E-5	7.472975851078537E-5	0.0	3.373973867003449	0.0010462166191509952
78-79	3.7364879255392686E-5	9.341219813848172E-5	0.0	3.7310700180472365	0.0010462166191509952
80-81	3.7364879255392686E-5	1.1209463776617806E-4	0.0	4.121831925300134	0.0010648990587786916
82-83	5.604731888308903E-5	1.4945951702157075E-4	0.0	4.525129749543215	0.0011209463776617806
84-85	7.472975851078537E-5	1.4945951702157075E-4	0.0	4.937376462367961	0.0011209463776617806
86-87	7.472975851078537E-5	1.4945951702157075E-4	0.0	5.376918219488774	0.0011209463776617806
88-89	7.472975851078537E-5	1.4945951702157075E-4	0.0	5.850854347964175	0.0011583112569171733
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3430	0.0	44.554356	1
GGTATCA	1785	0.0	44.13654	1
TCAACGC	4085	0.0	36.599857	4
CAACGCA	4225	0.0	35.385098	5
ATCAACG	4395	0.0	33.909668	3
TATCAAC	4475	0.0	33.72834	2
AACGCAG	4470	0.0	33.532383	6
GTACATG	7520	0.0	28.715868	1
ACGCAGA	5355	0.0	28.083395	7
TACATGG	7885	0.0	26.967283	2
CGCAGAG	5785	0.0	25.671764	8
ACATGGG	8215	0.0	25.190205	3
GTATATA	770	0.0	24.03822	1
TATCGCG	60	1.7012226E-4	23.77204	90-91
CATGGGG	7185	0.0	22.195932	4
GCAGAGT	6825	0.0	20.78659	9
GAGTACA	5750	0.0	20.552275	1
AGTACAT	5690	0.0	19.435894	2
AGAGTAC	5540	0.0	19.099142	10-11
ATGGGGG	4245	0.0	18.224115	5
>>END_MODULE