##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921690_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2676310
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.297487211870074	33.0	31.0	34.0	28.0	34.0
2	31.4323464023226	34.0	31.0	34.0	28.0	34.0
3	31.510048163329362	34.0	31.0	34.0	28.0	34.0
4	34.98308865564901	37.0	35.0	37.0	32.0	37.0
5	34.7143062649693	37.0	35.0	37.0	32.0	37.0
6	34.692454536283165	37.0	35.0	37.0	32.0	37.0
7	34.573634220251016	37.0	35.0	37.0	32.0	37.0
8	34.52114366422425	37.0	35.0	37.0	32.0	37.0
9	36.10667523567898	39.0	37.0	39.0	32.0	39.0
10-11	35.99141747405943	39.0	37.0	39.0	31.0	39.0
12-13	35.90645104640345	39.0	37.0	39.0	31.0	39.0
14-15	37.15164480198482	40.0	38.0	41.0	31.0	41.0
16-17	37.120387585892516	40.0	38.0	41.0	31.0	41.0
18-19	37.07346140768446	40.0	38.0	41.0	31.0	41.0
20-21	37.01596040817394	40.0	38.0	41.0	31.0	41.0
22-23	36.92210693081145	40.0	37.5	41.0	30.0	41.0
24-25	36.84273608064836	40.0	37.0	41.0	30.0	41.0
26-27	36.71689266191136	40.0	37.0	41.0	30.0	41.0
28-29	36.5961161076258	40.0	37.0	41.0	30.0	41.0
30-31	36.44134050240817	40.0	37.0	41.0	30.0	41.0
32-33	36.39516218225841	40.0	37.0	41.0	29.5	41.0
34-35	36.5331039752495	40.0	37.0	41.0	30.0	41.0
36-37	36.53814636570502	40.0	37.0	41.0	30.0	41.0
38-39	36.43368163628279	40.0	37.0	41.0	30.0	41.0
40-41	36.353126132622904	40.0	37.0	41.0	29.5	41.0
42-43	36.234205678714346	40.0	36.5	41.0	29.0	41.0
44-45	36.106564261987586	40.0	36.0	41.0	28.0	41.0
46-47	35.95680694687835	40.0	36.0	41.0	28.0	41.0
48-49	35.81038986515016	40.0	35.5	41.0	27.5	41.0
50-51	35.65472553627943	40.0	35.0	41.0	27.0	41.0
52-53	35.47631851317672	39.0	35.0	41.0	26.5	41.0
54-55	35.26057108481454	39.0	35.0	41.0	26.0	41.0
56-57	35.0303894167716	39.0	35.0	41.0	26.0	41.0
58-59	34.782249253636536	38.5	35.0	40.5	26.0	41.0
60-61	34.50205077139793	38.0	34.0	40.0	25.5	41.0
62-63	34.19443954549361	37.5	34.0	40.0	25.0	41.0
64-65	33.85521819221241	37.0	34.0	40.0	24.0	41.0
66-67	33.4976968288427	36.0	34.0	39.0	23.0	41.0
68-69	33.13486479518441	36.0	33.5	39.0	22.0	41.0
70-71	32.73917109751859	35.0	33.0	38.5	22.0	40.0
72-73	32.379771588493114	35.0	33.0	37.5	21.0	39.5
74-75	31.98338309089754	35.0	33.0	37.0	20.0	39.0
76-77	29.310386128662223	33.0	29.0	34.5	13.0	36.5
78-79	30.874153031599477	34.5	31.5	36.0	16.5	37.0
80-81	31.05976176152987	35.0	32.0	35.5	18.0	37.0
82-83	30.88834720193102	35.0	32.0	35.0	16.5	37.0
84-85	30.695420747222855	35.0	32.0	35.0	14.0	36.0
86-87	30.495055505528136	35.0	32.0	35.0	9.5	36.0
88-89	30.238254537030464	35.0	32.0	35.0	5.5	36.0
90-91	30.045288288725892	34.5	31.0	35.0	2.0	35.0
92-93	29.88869077199577	34.0	31.0	35.0	2.0	35.0
94-95	29.78804753559939	34.0	31.0	35.0	2.0	35.0
96-97	29.592666021499753	34.0	31.0	35.0	2.0	35.0
98-99	29.396054081926234	34.0	31.0	35.0	2.0	35.0
100-101	28.28295657080084	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	56051.0
3	18308.0
4	10141.0
5	6807.0
6	5897.0
7	6030.0
8	6105.0
9	6501.0
10	7202.0
11	7625.0
12	8337.0
13	8794.0
14	9293.0
15	10070.0
16	10601.0
17	11304.0
18	10518.0
19	9709.0
20	9284.0
21	9361.0
22	9811.0
23	11409.0
24	13322.0
25	15974.0
26	19367.0
27	23581.0
28	28338.0
29	34292.0
30	42046.0
31	51809.0
32	66266.0
33	87279.0
34	124074.0
35	187088.0
36	335099.0
37	655271.0
38	663517.0
39	79829.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.227160505839855	19.112291552133158	13.290327989039213	26.370219952987778
2	15.003441310522788	20.441160315748647	37.816266151927316	26.739132221801242
3	18.117904155322158	24.30219600768001	28.55381724773714	29.026082589260692
4	13.121223557898906	16.410525647109818	34.99362885958455	35.474621935406724
5	14.330829905836492	37.115857179430165	34.18574447847544	14.367568436257901
6	34.34584537755843	35.25603755705749	16.426429837593524	13.97168722779055
7	29.518665625432032	31.24843534568118	20.560772107864935	18.672126921021857
8	26.82271485739694	35.51572874592256	19.079179915630103	18.582376481050403
9	26.53127871258513	15.685034874523085	18.809375150627226	38.974311262264564
10-11	26.01146972216895	25.233669141019416	27.370120779366914	21.38474035744472
12-13	26.34887225855243	22.348387260698114	27.445180389870274	23.857560090879183
14-15	23.36474807146216	23.91276688405942	24.384501746982785	28.337983297495633
16-17	23.979121248869014	27.241356053924253	25.052184734239447	23.727337962967287
18-19	24.018966033917508	26.555044394075612	26.340491088676803	23.085498483330085
20-21	24.460023999556103	25.61508904365898	26.273797084369665	23.651089872415252
22-23	24.22544473547534	25.469527072723263	26.079452679248664	24.22557551255273
24-25	24.08841709192848	25.778067980237584	25.980157251122588	24.153357676711344
26-27	23.955997187096216	25.928093839187692	26.009308589146407	24.10660038456968
28-29	24.13697495822385	26.083171908490048	25.844883430337706	23.934969702948393
30-31	24.321177577871534	25.841153077387485	25.732197675854785	24.105471668886196
32-33	24.006109094116034	25.91863895900259	25.780485539948366	24.294766406933007
34-35	24.282263573353582	25.868723776668016	25.882774325760487	23.966238324217915
36-37	24.362277927139363	25.811793192225558	25.813680192660875	24.012248687974203
38-39	24.295148917726273	25.91469224417201	25.789052837675758	24.00110600042596
40-41	24.317350970710265	25.767143503846203	25.84841513774795	24.067090387695586
42-43	24.19232969652037	25.691081032906197	25.78930375652872	24.32728551404471
44-45	24.13958294019502	25.758347455172892	25.83876253449497	24.26330707013712
46-47	24.328014997311502	25.758076804798424	25.72540003355488	24.1885081643352
48-49	24.103078729973905	25.847277594652756	25.685361113363587	24.364282562009755
50-51	24.174308601389562	25.873463610540455	25.718881177412207	24.23334661065778
52-53	24.35893914893895	25.819566389132543	25.67174747477578	24.14974698715272
54-55	23.99057691808275	25.62838762279797	25.92201351583377	24.45902194328551
56-57	24.042031432022622	25.806974161020218	25.768559943275505	24.382434463681655
58-59	24.098808089720354	25.71883369218262	25.797330221126273	24.385027996970756
60-61	24.102439351586387	25.725154940929905	25.888340142681486	24.28406556480222
62-63	24.06103608792895	25.766665040941188	25.903300727259886	24.268998143869975
64-65	23.913371367695092	25.614469878561046	25.901475116462713	24.570683637281153
66-67	23.895383681277988	25.85662653890688	25.742614050810054	24.50537572900508
68-69	23.962725347290952	25.69372675310926	25.807859535554716	24.535688364045072
70-71	23.94802663914237	25.541735036514503	25.796812134520824	24.713426189822304
72-73	23.922952457521923	25.726918087087764	25.632148593184144	24.717980862206165
74-75	23.764579510963387	25.79944139360821	25.706340328309263	24.72963876711914
76-77	23.802095591931977	25.466596787388173	25.89710706902301	24.834200551656835
78-79	23.765013011869055	25.53003873831054	25.864450691205292	24.840497558615112
80-81	23.858810544191996	25.698271175464587	25.646766876024007	24.796151404319406
82-83	23.826248155785766	25.647916026233393	25.7333548861516	24.792480931829246
84-85	23.77889659516682	25.625587044231708	25.739178058634792	24.856338301966673
86-87	23.655696872719766	25.731296227435575	25.721929922465314	24.891076977379345
88-89	23.635017319269068	25.5513133583868	25.751908473466678	25.061760848877455
90-91	23.60016204369933	25.526773186789462	25.72676074495131	25.14630402455989
92-93	23.57919874777821	25.554786479100073	25.83187830838653	25.034136464735184
94-95	23.439026554873063	25.400023355209377	25.948272882266387	25.21267720765117
96-97	23.34064045842805	25.33116561102003	25.965282311512983	25.362911619038936
98-99	23.305889839168966	25.310570204167192	25.94288280741191	25.440657149251926
100-101	23.198488365315203	25.275043081737973	25.973000865371247	25.55346768757557
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	19.0
1	17.0
2	33.0
3	62.5
4	65.0
5	55.5
6	94.0
7	226.0
8	338.5
9	348.0
10	293.0
11	263.5
12	269.5
13	280.0
14	324.5
15	339.0
16	363.5
17	433.0
18	494.0
19	542.5
20	611.0
21	709.0
22	837.0
23	1043.5
24	1354.0
25	1755.5
26	2345.5
27	3298.0
28	4755.0
29	7062.5
30	10340.5
31	14677.0
32	20799.5
33	26144.5
34	30108.5
35	35090.0
36	42086.0
37	49508.0
38	56087.0
39	62249.5
40	68497.0
41	74503.5
42	80087.0
43	87297.5
44	94513.0
45	101746.0
46	107309.5
47	111402.5
48	116188.0
49	119043.5
50	119081.0
51	119248.5
52	119732.0
53	119093.0
54	117942.0
55	113769.0
56	105319.5
57	95608.5
58	86681.0
59	76175.5
60	62650.0
61	50421.5
62	41167.0
63	31411.5
64	22380.5
65	16004.5
66	11703.5
67	8986.0
68	6701.5
69	4729.0
70	3470.5
71	2471.0
72	1671.0
73	1197.0
74	868.0
75	587.0
76	377.5
77	237.5
78	121.5
79	59.5
80	44.5
81	34.5
82	24.5
83	11.5
84	5.0
85	3.0
86	4.0
87	3.0
88	2.5
89	1.5
90	1.0
91	2.5
92	2.0
93	1.0
94	1.0
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1583897231636096
2	1.4945951702157075E-4
3	0.01016324715746681
4	0.006725678265970684
5	0.02410034711972828
6	0.0023166225138343466
7	0.0
8	0.0
9	3.7364879255392686E-5
10-11	0.047341302016582536
12-13	3.549663529262305E-4
14-15	0.004651927467296389
16-17	1.8682439627696343E-5
18-19	0.0019429737212804197
20-21	3.9233123218162315E-4
22-23	0.0
24-25	6.53885386969372E-4
26-27	0.0026902713063882734
28-29	0.0053058128542657615
30-31	0.01660868882902205
32-33	0.01119078133699011
34-35	0.009640138847891312
36-37	0.003923312321816232
38-39	0.0
40-41	0.003717805485911572
42-43	0.009303854934592778
44-45	0.007286151454801573
46-47	0.0036243932877730906
48-49	0.008930206142038852
50-51	0.0025408117893667027
52-53	0.0023353049534620427
54-55	0.0043156435539978555
56-57	0.00812686123804791
58-59	0.014777809745507808
60-61	0.007827942204004767
62-63	0.021727677287010846
64-65	0.04100795498279347
66-67	0.05989590144639447
68-69	0.05064809383068478
70-71	0.06817222220146396
72-73	0.03254480983144703
74-75	0.02701480770164891
76-77	0.2238343091794299
78-79	0.08268847779218402
80-81	0.0663600255575774
82-83	0.02662247646946729
84-85	0.05090964798547253
86-87	0.06835904659774092
88-89	0.289596496668921
90-91	0.29495835684206984
92-93	0.051731675329091176
94-95	0.00924780761570969
96-97	0.0143107487548154
98-99	8.593922228740318E-4
100-101	7.472975851078537E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2676310.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.65443646175428
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.07168472313701	47.96048476963381
2	11.497203663854668	13.94712818234243
3	3.847193879348959	7.000481301330644
4	1.7257778817215865	4.187043394959315
5	0.9997417021804711	3.031938477653573
6	0.6247268273227454	2.273547219228048
7	0.43013925269231224	1.8262897780492626
8	0.3082559313159373	1.4957671839969127
9	0.2360760599927889	1.288715434287652
>10	1.1894506255631594	12.572653774471082
>50	0.051120577837132135	2.0896375414231425
>100	0.017145352574262387	1.6895762673573136
>500	0.0014217090232033248	0.5955698102012509
>1k	6.181343578744289E-5	0.041166865065704966
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.7364879255392686E-5
5	0.0	0.0	0.0	0.0	3.7364879255392686E-5
6	0.0	0.0	0.0	0.0	3.7364879255392686E-5
7	0.0	0.0	0.0	0.0	3.7364879255392686E-5
8	0.0	0.0	0.0	0.0	7.472975851078537E-5
9	0.0	0.0	0.0	0.0	7.472975851078537E-5
10-11	0.0	0.0	0.0	0.0	7.472975851078537E-5
12-13	0.0	0.0	0.0	0.0	1.307770773938744E-4
14-15	0.0	0.0	0.0	1.8682439627696343E-5	1.681419566492671E-4
16-17	0.0	0.0	0.0	5.604731888308903E-5	1.8682439627696343E-4
18-19	0.0	0.0	0.0	7.472975851078537E-5	1.8682439627696343E-4
20-21	0.0	0.0	0.0	1.307770773938744E-4	2.0550683590465977E-4
22-23	0.0	0.0	0.0	2.2418927553235612E-4	3.362839132985342E-4
24-25	0.0	0.0	0.0	3.923312321816232E-4	4.4837855106471224E-4
26-27	0.0	0.0	0.0	5.231083095754976E-4	4.4837855106471224E-4
28-29	0.0	0.0	0.0	0.001905608842025027	4.4837855106471224E-4
30-31	0.0	0.0	0.0	0.00807081391916482	4.4837855106471224E-4
32-33	0.0	0.0	0.0	0.021933184122915506	4.4837855106471224E-4
34-35	0.0	0.0	0.0	0.03962545445034395	4.857434303201049E-4
36-37	0.0	0.0	0.0	0.07017124324162746	4.857434303201049E-4
38-39	0.0	0.0	0.0	0.12427558840343607	5.044258699478013E-4
40-41	0.0	0.0	0.0	0.18891682951526542	5.604731888308903E-4
42-43	1.8682439627696343E-5	0.0	0.0	0.2520821578965068	5.97838068086283E-4
44-45	3.7364879255392686E-5	0.0	0.0	0.3300066135836282	6.912502662247647E-4
46-47	3.7364879255392686E-5	0.0	0.0	0.42009333746837996	7.09932705852461E-4
48-49	3.7364879255392686E-5	0.0	0.0	0.5219873631978358	7.472975851078537E-4
50-51	3.7364879255392686E-5	0.0	0.0	0.6340446360847585	7.846624643632464E-4
52-53	3.7364879255392686E-5	0.0	0.0	0.7490724168724848	8.407097832463354E-4
54-55	3.7364879255392686E-5	0.0	0.0	0.8745810462913489	9.341219813848172E-4
56-57	3.7364879255392686E-5	0.0	0.0	1.0198743792759433	9.901693002679062E-4
58-59	3.7364879255392686E-5	0.0	0.0	1.182318191838763	0.0010088517398956025
60-61	3.7364879255392686E-5	0.0	0.0	1.3634257615896515	0.0010462166191509952
62-63	3.7364879255392686E-5	0.0	0.0	1.5494094480833684	0.0010462166191509952
64-65	3.7364879255392686E-5	0.0	0.0	1.7396714132518283	0.0010462166191509952
66-67	3.7364879255392686E-5	0.0	0.0	1.9586856530073122	0.0010462166191509952
68-69	3.7364879255392686E-5	0.0	0.0	2.2097029118450404	0.0010462166191509952
70-71	3.7364879255392686E-5	0.0	0.0	2.4795894347067415	0.0011209463776617806
72-73	3.7364879255392686E-5	0.0	0.0	2.760778833543199	0.0013451356531941367
74-75	3.7364879255392686E-5	0.0	0.0	3.0512160399953667	0.0013451356531941367
76-77	3.7364879255392686E-5	0.0	0.0	3.37031210883642	0.0013451356531941367
78-79	3.7364879255392686E-5	0.0	0.0	3.728902855050424	0.0013451356531941367
80-81	3.7364879255392686E-5	0.0	0.0	4.121607736024601	0.001363818092821833
82-83	3.7364879255392686E-5	0.0	0.0	4.525671540292418	0.001419865411704922
84-85	3.7364879255392686E-5	0.0	0.0	4.939711767321424	0.0014572302909603148
86-87	3.7364879255392686E-5	0.0	0.0	5.380075551785854	0.0014945951702157075
88-89	3.7364879255392686E-5	1.8682439627696343E-5	0.0	5.856664586688388	0.0015319600494711001
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1865	0.0	43.57923	1
GTATCAA	3300	0.0	41.91228	1
TCAACGC	4035	0.0	33.0472	4
CAACGCA	4120	0.0	32.5067	5
ATCAACG	4160	0.0	32.0536	3
AACGCAG	4465	0.0	30.203764	6
TATCAAC	4440	0.0	30.139076	2
GTACATG	7135	0.0	26.312698	1
TACATGG	7605	0.0	24.272482	2
ACGCAGA	5515	0.0	24.021858	7
ACATGGG	8110	0.0	22.351482	3
CGCAGAG	5855	0.0	22.30251	8
GTATATA	860	0.0	22.106693	1
CATGGGG	7015	0.0	19.820375	4
TATACCG	175	9.9558056E-5	18.996826	5
AGAGTAC	5575	0.0	18.397758	10-11
GCAGAGT	6925	0.0	18.10257	9
GAGTACA	5630	0.0	17.390862	1
GTATAAT	910	0.0	17.235933	1
TATACTG	1135	0.0	17.155724	5
>>END_MODULE