FastQCFastQC Report
Mon 15 Aug 2016
SRR2921689_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921689_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3180157
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA23550.049.1740651
GTATCAA41800.038.3775831
TCAACGC48300.031.8427284
CAACGCA48850.031.7742335
ATCAACG50450.030.5773893
AACGCAG52900.029.2316366
TATCAAC53850.029.1761052
GTACATG79750.026.1853961
ACGCAGA62250.024.0828157
TACATGG84300.023.7612842
CGCAGAG63850.023.1835828
ACATGGG85200.022.507123
CATGGGG72550.021.0029354
GAGTACA61700.018.1536521
TAAGGCG10750.018.1036975
AGAGTAC63250.018.07841910-11
GCAGAGT77100.017.9076719
TATACCG3208.358256E-917.8001295
ATGGGGG41000.017.3659785
TATAGGG6300.016.575522
Produced by FastQC (version 0.11.5)