##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921689_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3180157
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.31973641552917	31.0	31.0	34.0	26.0	34.0
2	30.394805665254893	31.0	31.0	34.0	26.0	34.0
3	30.450962012252855	33.0	31.0	34.0	26.0	34.0
4	33.907285080579356	37.0	35.0	37.0	30.0	37.0
5	33.90464024260437	37.0	35.0	37.0	30.0	37.0
6	33.944743294120386	37.0	35.0	37.0	30.0	37.0
7	33.855330412932446	37.0	35.0	37.0	29.0	37.0
8	33.82725695618172	37.0	35.0	37.0	29.0	37.0
9	35.37721093644119	39.0	35.0	39.0	29.0	39.0
10-11	35.27245227201047	39.0	35.0	39.0	29.0	39.0
12-13	35.167095681125176	39.0	35.0	39.0	28.0	39.0
14-15	36.29410780662715	40.0	36.5	41.0	28.0	41.0
16-17	36.23364664071617	40.0	36.0	41.0	27.0	41.0
18-19	36.207102510976654	40.0	36.5	41.0	27.0	41.0
20-21	36.13291230590188	40.0	36.0	41.0	27.0	41.0
22-23	36.03160598674845	40.0	36.0	41.0	27.0	41.0
24-25	35.920373270879395	40.0	36.0	41.0	27.0	41.0
26-27	35.77606310002933	40.0	36.0	41.0	27.0	41.0
28-29	35.63823043956634	40.0	36.0	41.0	26.0	41.0
30-31	35.49492053379754	40.0	36.0	41.0	25.0	41.0
32-33	35.32418603861382	39.0	35.0	41.0	25.0	41.0
34-35	35.150901354870214	39.0	35.0	41.0	24.5	41.0
36-37	35.11518236363803	39.0	35.0	41.0	24.5	41.0
38-39	35.296115569137	39.5	35.0	41.0	25.0	41.0
40-41	35.3607801438734	40.0	35.0	41.0	24.5	41.0
42-43	35.204794134377636	40.0	35.0	41.0	24.0	41.0
44-45	35.08547659753905	40.0	35.0	41.0	23.5	41.0
46-47	34.93364620048633	40.0	35.0	41.0	23.0	41.0
48-49	34.777789587117866	39.0	35.0	41.0	22.0	41.0
50-51	33.1043569547038	37.0	32.5	39.5	18.0	40.0
52-53	33.225991672738175	37.5	33.0	39.5	17.5	40.0
54-55	34.14373598536173	39.0	34.0	40.5	20.0	41.0
56-57	34.16998657613445	39.0	34.0	41.0	19.0	41.0
58-59	33.99115358141123	38.5	34.0	41.0	18.0	41.0
60-61	33.74911647443821	38.0	34.0	40.5	16.5	41.0
62-63	33.46887339209982	37.5	34.0	40.0	13.5	41.0
64-65	33.18852088120178	37.0	33.0	40.0	12.5	41.0
66-67	32.853682066640104	36.5	33.0	39.5	9.0	41.0
68-69	32.49722042024969	36.0	33.0	39.0	7.5	41.0
70-71	32.092400469536564	35.5	32.0	39.0	4.5	41.0
72-73	31.731642651604936	35.0	32.0	38.0	2.0	40.0
74-75	31.334155043288746	35.0	32.0	37.0	2.0	39.0
76-77	30.95188083481413	35.0	32.0	37.0	2.0	39.0
78-79	30.602687068594413	35.0	31.0	36.0	2.0	39.0
80-81	30.28355722689163	35.0	31.0	36.0	2.0	37.0
82-83	29.969158755369627	35.0	31.0	35.0	2.0	37.0
84-85	29.70654027458393	35.0	31.0	35.0	2.0	36.5
86-87	29.471940693494062	35.0	31.0	35.0	2.0	36.0
88-89	29.254757548133632	35.0	30.5	35.0	2.0	36.0
90-91	29.032824637274196	34.0	30.0	35.0	2.0	35.5
92-93	28.847653590687504	34.0	30.0	35.0	2.0	35.0
94-95	28.62424622432163	34.0	30.0	35.0	2.0	35.0
96-97	28.397365601761173	34.0	30.0	35.0	2.0	35.0
98-99	28.13883182496965	34.0	29.5	35.0	2.0	35.0
100-101	27.07412307002453	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	109685.0
3	18644.0
4	13116.0
5	9692.0
6	8998.0
7	9110.0
8	9840.0
9	10582.0
10	11644.0
11	12430.0
12	12206.0
13	12495.0
14	13027.0
15	13957.0
16	13646.0
17	14314.0
18	13966.0
19	13248.0
20	13220.0
21	14139.0
22	15387.0
23	17703.0
24	20719.0
25	24421.0
26	28822.0
27	34203.0
28	40617.0
29	48033.0
30	56761.0
31	70083.0
32	86583.0
33	111177.0
34	152607.0
35	221436.0
36	365796.0
37	663895.0
38	739713.0
39	134227.0
40	15.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01880191071059	18.540732858571296	13.517157317730833	24.923307912987283
2	15.559634113105702	19.71120287865599	39.812921348668205	24.9162416595701
3	18.594756669340498	24.133425517030645	29.70356353767631	27.568254275952548
4	13.17864780867768	16.288176791129228	37.055356740501665	33.47781865969143
5	14.257272143693282	37.1614094931212	34.62537918360668	13.955939179578838
6	33.00161362705056	35.69339262021144	17.538311456123086	13.766682296614905
7	28.49689244090417	31.690498310199878	21.73231402291939	18.080295225976563
8	26.123098029237646	34.90181413548098	20.370582957046178	18.604504878235186
9	26.6939105386299	15.607059297089235	19.734270044529424	37.96476011975144
10-11	25.3576863769868	25.286987238616828	28.31907766558238	21.03624871881399
12-13	25.940031364783252	22.902126275649014	27.84464304473665	23.31319931483109
14-15	23.236487567355518	24.431092928553486	25.042402694016214	27.290016810074786
16-17	23.50198641584402	27.236237458985595	25.952069667435566	23.309706457734816
18-19	23.4401891071549	26.85358080083729	27.021862645094348	22.684367446913463
20-21	24.052475841688043	26.107111809486188	26.839645548089543	23.00076680073623
22-23	23.936704492512582	25.929946109730288	26.455337703653537	23.67801169410359
24-25	23.7601463873136	26.076891716924127	26.50325703594001	23.659704859822256
26-27	23.552784723990335	26.265820445774118	26.515113509963623	23.66628132027192
28-29	23.6954337389751	26.2895492863399	26.445678649321408	23.569338325363592
30-31	23.916776699438273	26.145358991450387	26.277256796125588	23.660607512985756
32-33	23.70252032526717	26.251541937152645	26.116960011447087	23.928977726133095
34-35	24.051143225654695	26.303490455810692	26.144998469325902	23.50036784920871
36-37	24.134210738984816	25.97902351882767	26.241355314384652	23.64541042780286
38-39	23.827132230994337	26.19175126241106	26.20893665649275	23.772179850101853
40-41	24.10676537173909	26.0320740124527	26.238670412235287	23.622490203572923
42-43	23.96106346099837	25.98180578569837	26.112063903229803	23.945066850073456
44-45	23.861363129688666	26.07905833937942	26.230434916185935	23.82914361474598
46-47	24.029865608856763	25.9907908017681	26.020554310288595	23.95878927908654
48-49	23.81942551357586	26.13325475600321	26.091071966014255	23.956247764406676
50-51	23.893471784467987	26.14933727107318	26.130272509496145	23.826918434962693
52-53	24.065925563977427	25.983906551036203	26.166091187806963	23.784076697179415
54-55	23.68886910553413	25.939092653066048	26.385510180768218	23.986528060631606
56-57	23.724473395605642	26.053571203469094	26.236748225743423	23.985207175181838
58-59	23.800647962357104	25.86578174740562	26.36291848750792	23.97065180272936
60-61	23.757648154553987	25.98819475579881	26.46341453805344	23.790742551593773
62-63	23.779214282056433	25.959024175027672	26.43314937149655	23.82861217141934
64-65	23.58552865341184	25.828227171331473	26.408932788374923	24.177311386881765
66-67	23.607668543313643	25.87264399553588	26.319906885620544	24.199780575529932
68-69	23.728349705189988	25.815991853749402	26.38979330133801	24.065865139722597
70-71	23.617469702994505	25.69526672896949	26.3617819447429	24.325481623293104
72-73	23.534827247938544	25.8167281839487	26.314796006633973	24.333648561478785
74-75	23.625054535536844	25.889841130437972	26.292628118924576	24.192476215100605
76-77	23.617714156074136	25.622333486680827	26.42483977287766	24.33511258436738
78-79	23.5558082436008	25.684113224904735	26.397823885291956	24.36225464620251
80-81	23.610514645218586	25.799823332274798	26.297723728555795	24.291938293950825
82-83	23.662000597921647	25.707368572677186	26.386499767623757	24.24413106177741
84-85	23.54889315247667	25.766669687072792	26.38845837158087	24.29597878886967
86-87	23.573569147939317	25.76475481836871	26.34238060254217	24.319295431149804
88-89	23.439013511575386	25.570683994937692	26.428550039165128	24.561752454321798
90-91	23.397128696407364	25.613377245348534	26.496440620663076	24.493053437581025
92-93	23.53620844819135	25.449746957740132	26.603654336865368	24.410390257203154
94-95	23.350101071396203	25.373656814339924	26.653264034738612	24.62297807952526
96-97	23.42468003382036	25.190791203428443	26.680299263505603	24.7042294992456
98-99	23.23528577327907	25.17287305305034	26.626926459601485	24.964914714069106
100-101	23.391659570819183	25.06698364429486	26.747162633041228	24.794194151844724
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	40.0
1	38.0
2	53.5
3	71.0
4	66.5
5	73.0
6	123.0
7	274.5
8	428.0
9	464.0
10	455.0
11	456.5
12	506.5
13	579.0
14	673.0
15	806.0
16	891.5
17	966.0
18	1073.0
19	1184.0
20	1270.5
21	1341.5
22	1486.0
23	1618.0
24	1825.0
25	2203.5
26	2787.0
27	3759.5
28	5309.0
29	7887.0
30	12010.5
31	17862.0
32	25726.5
33	33238.0
34	40201.5
35	48723.5
36	59630.5
37	70160.0
38	79548.0
39	88763.5
40	96422.0
41	103656.0
42	110903.5
43	117905.5
44	124089.5
45	130137.5
46	133876.0
47	136297.5
48	138734.0
49	139145.5
50	137174.0
51	134710.0
52	132485.0
53	129095.0
54	125505.5
55	118588.5
56	108305.0
57	97321.0
58	87520.0
59	76155.5
60	62222.5
61	51615.0
62	43053.0
63	33141.5
64	24122.0
65	17946.5
66	13512.0
67	10407.5
68	7906.5
69	5970.5
70	4626.5
71	3416.0
72	2481.5
73	1857.5
74	1434.0
75	1046.0
76	715.0
77	469.5
78	312.0
79	239.5
80	171.5
81	136.0
82	121.0
83	100.0
84	93.5
85	75.5
86	58.5
87	50.0
88	48.0
89	44.5
90	35.5
91	28.0
92	23.0
93	20.5
94	18.5
95	19.0
96	15.0
97	8.5
98	6.5
99	5.5
100	3.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05858201340374076
2	0.08059350528920428
3	0.07685155166867548
4	0.07675721670345206
5	0.07160023860457204
6	0.011445975780441028
7	0.015439489308232267
8	0.019684562743285946
9	0.00745246225264979
10-11	0.015282264366193243
12-13	0.024998765784204995
14-15	0.04429026617239337
16-17	0.029511121620725014
18-19	0.011713258181907372
20-21	0.02242027673476498
22-23	0.04056403504606848
24-25	0.037513871170511394
26-27	0.02410258361458255
28-29	0.01326978510809372
30-31	0.013285507602297622
32-33	0.009732223912215655
34-35	0.005833045349647832
36-37	7.546797217873205E-4
38-39	0.004119293481422458
40-41	0.0013678569957395186
42-43	0.23929636178339625
44-45	0.20799287582342632
46-47	0.2140774810803366
48-49	0.2220645081359191
50-51	0.2126624566019854
52-53	0.2201935313256547
54-55	0.21679747257761173
56-57	0.22002058388941176
58-59	0.22915535302187912
60-61	0.23314886654967035
62-63	0.218369721998002
64-65	0.21849550195163323
66-67	0.21557111802970735
68-69	0.209816685151079
70-71	0.20159382068243803
72-73	0.21363725124262734
74-75	0.2128039590498205
76-77	0.20555588922182144
78-79	0.22522472947090347
80-81	0.23692226515860695
82-83	0.23585313555274157
84-85	0.22937546794073374
86-87	0.24990904537103042
88-89	0.24187485083283625
90-91	0.2485569108694948
92-93	0.2440760000213826
94-95	0.24824246098541675
96-97	0.25479874106844413
98-99	0.2480066235723582
100-101	0.25514463594092995
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3180157.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.29899266716773
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.66803506675612	46.833042769636535
2	12.445662453070405	15.009218179914743
3	4.171921418414188	7.546879770508713
4	1.8881728678940852	4.55419687661962
5	1.0522163458666407	3.17237928618433
6	0.6389226400231877	2.3115834951387386
7	0.4461144382389177	1.8830175868060306
8	0.3032616452407386	1.4629097378083618
9	0.23281812511028896	1.2634828576928148
>10	1.0852876314514672	11.422798008803905
>50	0.05035913874878202	2.062991898784546
>100	0.01560979191706913	1.5769314524692724
>500	0.001096360730456173	0.4870062641414803
>1k	5.220765375917855E-4	0.413561815491053
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.1444988407805024E-5
2	0.0	0.0	0.0	0.0	3.1444988407805024E-5
3	0.0	0.0	0.0	0.0	3.1444988407805024E-5
4	0.0	0.0	0.0	0.0	3.1444988407805024E-5
5	0.0	0.0	0.0	0.0	3.1444988407805024E-5
6	0.0	0.0	0.0	0.0	9.433496522341507E-5
7	0.0	0.0	0.0	0.0	9.433496522341507E-5
8	0.0	0.0	0.0	0.0	1.257799536312201E-4
9	0.0	0.0	0.0	0.0	1.257799536312201E-4
10-11	0.0	0.0	0.0	0.0	1.257799536312201E-4
12-13	0.0	0.0	0.0	0.0	2.9872738987414776E-4
14-15	0.0	0.0	0.0	0.0	4.559523319131728E-4
16-17	0.0	0.0	0.0	0.0	5.660097913404904E-4
18-19	0.0	0.0	0.0	0.0	6.288997681561005E-4
20-21	0.0	0.0	0.0	0.0	7.232347333795155E-4
22-23	0.0	0.0	0.0	0.0	8.175696986029306E-4
24-25	0.0	0.0	0.0	4.716748261170753E-5	9.119046638263457E-4
26-27	0.0	0.0	0.0	3.144498840780502E-4	9.119046638263457E-4
28-29	0.0	0.0	0.0	0.001226354547904396	9.119046638263457E-4
30-31	0.0	0.0	0.0	0.005974547797482955	9.119046638263457E-4
32-33	0.0	0.0	0.0	0.019873232673732776	9.119046638263457E-4
34-35	0.0	0.0	0.0	0.03966785287644604	9.747946406419557E-4
36-37	0.0	0.0	0.0	0.07518496728306182	9.747946406419557E-4
38-39	0.0	0.0	0.0	0.13357831075635573	9.747946406419557E-4
40-41	0.0	0.0	0.0	0.1995184514475229	9.747946406419557E-4
42-43	0.0	0.0	0.0	0.2708985751332403	0.0010062396290497608
44-45	0.0	0.0	0.0	0.36067401703752366	0.0010691296058653707
46-47	0.0	0.0	0.0	0.4679014275081388	0.0011162970884770783
48-49	0.0	0.0	0.0	0.5799870886877597	0.0011949095594965908
50-51	0.0	0.0	0.0	0.6976070678271544	0.0011949095594965908
52-53	0.0	0.0	0.0	0.8209185898683619	0.001352134501535616
54-55	0.0	0.0	0.0	0.9605186159048122	0.0013835794899434211
56-57	0.0	0.0	0.0	1.1255576375631768	0.0014150244783512262
58-59	0.0	0.0	0.0	1.3004232180989805	0.001446469466759031
60-61	0.0	0.0	0.0	1.4871435592645268	0.0014936369493707387
62-63	0.0	0.0	0.0	1.681457865130558	0.0015722494203902512
64-65	0.0	0.0	0.0	1.895944131060196	0.0016036944087980563
66-67	0.0	3.1444988407805024E-5	0.0	2.146576411164606	0.0016508618914097637
68-69	0.0	3.1444988407805024E-5	0.0	2.423025026751824	0.0016665843856136662
70-71	0.0	3.1444988407805024E-5	0.0	2.706690267178633	0.0016980293740214713
72-73	0.0	3.1444988407805024E-5	0.0	3.0068484040253356	0.0017923643392448864
74-75	0.0	3.1444988407805024E-5	0.0	3.3227133125817376	0.001933866787080009
76-77	0.0	3.1444988407805024E-5	0.0	3.679157978678411	0.0020596467407112294
78-79	0.0	3.1444988407805024E-5	0.0	4.076402517234212	0.002106814223322937
80-81	0.0	3.1444988407805024E-5	0.0	4.494337858162348	0.002153981705934644
82-83	0.0	4.716748261170753E-5	0.0	4.928939042946622	0.0022168716827502544
84-85	3.1444988407805024E-5	6.288997681561005E-5	0.0	5.3928155119385615	0.0022325941769541567
86-87	3.1444988407805024E-5	6.288997681561005E-5	0.0	5.888451419222385	0.002311206647973669
88-89	3.1444988407805024E-5	6.288997681561005E-5	0.0	6.4288492675047175	0.002405541613197084
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2355	0.0	49.174065	1
GTATCAA	4180	0.0	38.377583	1
TCAACGC	4830	0.0	31.842728	4
CAACGCA	4885	0.0	31.774233	5
ATCAACG	5045	0.0	30.577389	3
AACGCAG	5290	0.0	29.231636	6
TATCAAC	5385	0.0	29.176105	2
GTACATG	7975	0.0	26.185396	1
ACGCAGA	6225	0.0	24.082815	7
TACATGG	8430	0.0	23.761284	2
CGCAGAG	6385	0.0	23.183582	8
ACATGGG	8520	0.0	22.50712	3
CATGGGG	7255	0.0	21.002935	4
GAGTACA	6170	0.0	18.153652	1
TAAGGCG	1075	0.0	18.103697	5
AGAGTAC	6325	0.0	18.078419	10-11
GCAGAGT	7710	0.0	17.907671	9
TATACCG	320	8.358256E-9	17.800129	5
ATGGGGG	4100	0.0	17.365978	5
TATAGGG	630	0.0	16.57552	2
>>END_MODULE