FastQCFastQC Report
Mon 15 Aug 2016
SRR2921689_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921689_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3180157
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24250.046.2710881
GTATCAA42150.039.818651
TCAACGC50700.032.1097264
CAACGCA52650.030.8550835
ATCAACG53400.030.4866813
AACGCAG56650.028.6683146
TATCAAC57700.028.4614852
GTACATG77400.024.8170321
ACGCAGA67050.023.9379567
TACATGG81350.023.0460682
ACATGGG83200.022.5336253
CGCAGAG71450.022.0650528
CATGGGG67950.020.815044
AGAGTAC68650.018.2619110-11
GCAGAGT84550.017.8044459
ATGGGGG38650.016.2227125
ACTATAC7950.016.1195873
CAGAGTA75300.016.08155310-11
TAAGGTG16000.016.031455
TAATACT25750.015.6672274
Produced by FastQC (version 0.11.5)