##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921689_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3180157
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.50521342185307	33.0	31.0	34.0	30.0	34.0
2	31.63901562092689	34.0	31.0	34.0	30.0	34.0
3	31.708202456671163	34.0	31.0	34.0	29.0	34.0
4	35.19519602334098	37.0	35.0	37.0	33.0	37.0
5	34.95558363942409	37.0	35.0	37.0	32.0	37.0
6	34.937506544488215	37.0	35.0	37.0	32.0	37.0
7	34.82994613159036	37.0	35.0	37.0	32.0	37.0
8	34.77810372255206	37.0	35.0	37.0	32.0	37.0
9	36.38158965107697	39.0	37.0	39.0	32.0	39.0
10-11	36.27743410152392	39.0	37.0	39.0	32.0	39.0
12-13	36.21118705774589	39.0	37.0	39.0	32.0	39.0
14-15	37.47074609838445	40.0	38.0	41.0	32.0	41.0
16-17	37.440899301512474	40.0	38.0	41.0	32.0	41.0
18-19	37.40391071887331	40.0	38.0	41.0	32.0	41.0
20-21	37.3508392824631	40.0	38.0	41.0	31.5	41.0
22-23	37.263437779958664	40.0	38.0	41.0	31.0	41.0
24-25	37.182576520593166	40.0	38.0	41.0	31.0	41.0
26-27	37.05854883894097	40.0	37.0	41.0	31.0	41.0
28-29	36.94292640269018	40.0	37.0	41.0	30.0	41.0
30-31	36.788056375833015	40.0	37.0	41.0	30.0	41.0
32-33	36.74093496012932	40.0	37.0	41.0	30.0	41.0
34-35	36.87593112541299	40.0	37.0	41.0	30.0	41.0
36-37	36.8763889644442	40.0	37.0	41.0	30.0	41.0
38-39	36.77222665421864	40.0	37.0	41.0	30.0	41.0
40-41	36.697928750058566	40.0	37.0	41.0	30.0	41.0
42-43	36.58004494746643	40.0	37.0	41.0	30.0	41.0
44-45	36.455210072961805	40.0	37.0	41.0	30.0	41.0
46-47	36.3076096557497	40.0	36.0	41.0	29.5	41.0
48-49	36.16881745146544	40.0	36.0	41.0	29.0	41.0
50-51	36.018566850630336	40.0	36.0	41.0	28.0	41.0
52-53	35.85244722194533	39.5	35.0	41.0	28.0	41.0
54-55	35.64677828799019	39.0	35.0	41.0	28.0	41.0
56-57	35.42963979451329	39.0	35.0	41.0	27.5	41.0
58-59	35.19619660287212	39.0	35.0	41.0	27.0	41.0
60-61	34.92718692819254	38.0	35.0	40.0	26.0	41.0
62-63	34.6304017380274	38.0	34.0	40.0	26.0	41.0
64-65	34.30112664248966	37.0	34.0	40.0	26.0	41.0
66-67	33.954521427715676	37.0	34.0	39.5	26.0	41.0
68-69	33.59091988225738	36.0	34.0	39.0	25.0	41.0
70-71	33.193108390560596	35.5	33.5	39.0	24.5	40.0
72-73	32.832185486439826	35.0	33.0	38.0	24.0	40.0
74-75	32.4240672708926	35.0	33.0	37.0	22.5	39.0
76-77	29.688637542108772	33.0	29.0	34.5	18.5	36.5
78-79	31.262579646225014	34.5	31.5	36.0	20.0	38.0
80-81	31.438467817783838	35.0	32.0	36.0	20.0	37.0
82-83	31.256986526136917	35.0	32.0	35.0	20.0	37.0
84-85	31.050145951913695	35.0	32.0	35.0	20.0	36.5
86-87	30.830853319505923	35.0	32.0	35.0	18.5	36.0
88-89	30.55251061504196	35.0	32.0	35.0	16.0	36.0
90-91	30.352144249482023	34.5	32.0	35.0	11.0	35.5
92-93	30.195680118937524	34.0	32.0	35.0	8.5	35.0
94-95	30.090873658124423	34.0	31.5	35.0	2.0	35.0
96-97	29.889856695754332	34.0	31.0	35.0	2.0	35.0
98-99	29.687193588241083	34.0	31.0	35.0	2.0	35.0
100-101	28.565089710979677	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	50101.0
3	17396.0
4	9701.0
5	6889.0
6	6197.0
7	6248.0
8	6545.0
9	7092.0
10	7883.0
11	8822.0
12	9350.0
13	9925.0
14	11013.0
15	11671.0
16	12670.0
17	13230.0
18	12721.0
19	11535.0
20	11025.0
21	11221.0
22	11697.0
23	13330.0
24	16099.0
25	19077.0
26	22724.0
27	27769.0
28	33368.0
29	40525.0
30	49424.0
31	61683.0
32	78670.0
33	103105.0
34	146618.0
35	220021.0
36	387046.0
37	757813.0
38	842543.0
39	107403.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.92629917470069	20.079994279823115	14.177848993000028	25.815857552476167
2	14.787091937743856	21.03732777552771	38.298483846055156	25.877096440673274
3	17.27081633795482	25.30547653206851	29.517269401148376	27.906437728828294
4	12.815844911967995	17.535550389113194	36.142761947380485	33.505842751538324
5	13.968040482223604	37.436085997045865	34.6976917986256	13.898181722104935
6	32.85912124278557	36.36742700345463	17.234712815581485	13.538738938178325
7	28.18794795351299	31.947070537712445	21.781283125329974	18.08369838344459
8	26.10795630530191	35.38642903479294	20.11212025066687	18.393494409238286
9	26.394797489557902	15.721204959377792	19.700159457536216	38.18383809352809
10-11	25.254277547848602	25.601782593443794	28.27608175515925	20.867858103548357
12-13	25.782982919652113	23.08949023095166	27.954266879570937	23.173259969825285
14-15	23.083917561693823	24.601207932885067	25.09938748003917	27.215487025381936
16-17	23.340973942634584	27.5410817046268	25.93638080390069	23.18156354883793
18-19	23.320247778605495	27.072413199974715	27.011676058937823	22.595662962481963
20-21	23.92164199930978	26.24972583818832	26.87622193624667	22.95241022625523
22-23	23.811123760733157	26.05148520206474	26.511336784840623	23.62605425236148
24-25	23.629624924492152	26.193417067776725	26.574203028615468	23.60275497911565
26-27	23.459773427627642	26.442798659829652	26.55208961764202	23.545338294900684
28-29	23.56926164539117	26.49180851642589	26.448443041067637	23.490486797115302
30-31	23.793769072122796	26.32134969103259	26.304507645360932	23.58037359148368
32-33	23.626788170142184	26.376089444437977	26.15487478917149	23.842247596248345
34-35	23.936200239481035	26.402666211710358	26.19307706399081	23.468056484817797
36-37	24.003152189858433	26.12650929092552	26.292687935248033	23.577650583968012
38-39	23.729303888031925	26.33346817994649	26.237450892746946	23.69977703927464
40-41	23.958082090549603	26.162435829907	26.281349989386875	23.598132090156522
42-43	23.8728544291049	26.116413883674173	26.110753213491705	23.89997847372922
44-45	23.79843709437734	26.177167564936184	26.217027177337226	23.807368163349253
46-47	23.97009198378499	26.144923124624807	25.999845283845968	23.885139607744236
48-49	23.722632902093263	26.263844328393226	26.10924447972245	23.904278289791066
50-51	23.8279871165649	26.236076291193307	26.104789150493584	23.83114744174821
52-53	24.031159617965287	26.095904895399563	26.113058565396596	23.759876921238554
54-55	23.665710429017633	26.068794079240625	26.310854017966996	23.954641473774743
56-57	23.659664863148	26.13721522251521	26.161854400852857	24.041265513483935
58-59	23.69128144727645	26.007574786669192	26.29918127202007	24.001962494034284
60-61	23.692494877592907	26.125966480408273	26.357231505441288	23.824307136557533
62-63	23.704277534205602	26.09981369205358	26.317479623207234	23.87842915053358
64-65	23.504283990371245	25.974813861278996	26.236263736263737	24.284638412086025
66-67	23.525952459128067	26.057819877327177	26.17935366678849	24.236873996756266
68-69	23.619712775292143	26.01350894309148	26.241101505213017	24.125676776403367
70-71	23.510828159366312	25.916968666064477	26.171511952190297	24.400691222378914
72-73	23.509473181574112	26.026784295949675	26.08228870028471	24.381453822191506
74-75	23.513289679212036	26.1229119970408	26.030075169050154	24.33372315469701
76-77	23.56696661381331	25.859744384324035	26.18011107242938	24.39317792943328
78-79	23.44671176121793	25.92673897909058	26.185321358476504	24.441227901214983
80-81	23.584216352698398	26.049034557802237	26.04030243662368	24.32644665287568
82-83	23.61001764263119	25.98480575963181	26.10283744716883	24.302339150568173
84-85	23.540071837229355	26.057332294457446	26.02266114885767	24.37993471945553
86-87	23.50100774571767	26.100677022610952	25.991893979801027	24.40642125187035
88-89	23.37216600850355	25.94026381196301	26.070813514843366	24.616756664690076
90-91	23.3434268402119	25.990243994757684	26.088671457928214	24.5776577071022
92-93	23.414799208345443	25.850610990129802	26.211924119838038	24.52266568168672
94-95	23.26439026384542	25.76962841187214	26.235349892563914	24.730631431718532
96-97	23.260370567632673	25.628770970760645	26.306773213470535	24.804085248136147
98-99	23.0708916322092	25.69003107893282	26.16843941997941	25.07063786887857
100-101	23.040769749370472	25.68270321670159	26.245742341875484	25.030784692052453
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	54.0
1	40.0
2	39.5
3	51.0
4	44.5
5	49.0
6	82.5
7	226.5
8	341.5
9	348.0
10	318.5
11	272.0
12	271.5
13	303.0
14	362.0
15	420.0
16	459.0
17	506.0
18	558.0
19	600.5
20	682.0
21	814.0
22	969.0
23	1237.5
24	1563.5
25	2017.0
26	2773.5
27	3941.0
28	5813.0
29	8756.5
30	13199.0
31	19236.0
32	27373.0
33	35053.0
34	41442.5
35	49446.5
36	60091.5
37	70858.5
38	80706.5
39	89719.5
40	96764.0
41	104066.5
42	111580.0
43	118329.5
44	124311.0
45	130455.5
46	134565.5
47	137260.0
48	139772.0
49	139800.5
50	137910.0
51	135795.0
52	132890.0
53	129306.5
54	125456.5
55	118007.5
56	107382.0
57	96238.0
58	86775.5
59	75715.5
60	61935.0
61	51146.5
62	42261.0
63	31917.5
64	23057.5
65	17045.5
66	12663.5
67	9619.5
68	7261.0
69	5566.0
70	4239.5
71	3048.5
72	2158.0
73	1525.5
74	1113.0
75	812.5
76	535.5
77	321.5
78	196.0
79	112.0
80	67.5
81	43.5
82	27.5
83	22.5
84	16.5
85	10.0
86	9.5
87	5.5
88	4.5
89	4.0
90	1.5
91	1.5
92	3.0
93	3.5
94	2.0
95	0.5
96	1.0
97	2.0
98	1.5
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17074628705438127
2	1.5722494203902513E-4
3	0.011666090699295664
4	0.007358127287426375
5	0.027828814740907443
6	0.0023583741305853766
7	0.0
8	0.0
9	0.0
10-11	0.05163267096561585
12-13	3.458948724858553E-4
14-15	0.005628652924997099
16-17	1.5722494203902512E-5
18-19	0.0018866993044683016
20-21	2.987273898741477E-4
22-23	1.5722494203902512E-5
24-25	7.546797217873205E-4
26-27	0.00356900618428587
28-29	0.0065405575888234455
30-31	0.019322945376596186
32-33	0.01383579489943421
34-35	0.011461698274644931
36-37	0.004575245813335631
38-39	1.5722494203902512E-5
40-41	0.004150738469830263
42-43	0.010109563773109315
44-45	0.007751189642523939
46-47	0.004402298377092703
48-49	0.010534071116614682
50-51	0.0032860012886156247
52-53	0.0024998765784204993
54-55	0.005015475651044901
56-57	0.008238586962844916
58-59	0.01665012136193276
60-61	0.008034194538194183
62-63	0.023379348881203035
64-65	0.042293509408497756
66-67	0.06378615898523249
68-69	0.05251313064103439
70-71	0.0725907557394179
72-73	0.035532836900819674
74-75	0.02902372430040404
76-77	0.2371738250658694
78-79	0.08908365215931163
80-81	0.07027954909144424
82-83	0.029118059265627452
84-85	0.05413254754403635
86-87	0.07144301366253301
88-89	0.30555095235864144
90-91	0.30822377637330484
92-93	0.05504445220786269
94-95	0.011304473332605905
96-97	0.01639856145467032
98-99	8.961821696224432E-4
100-101	1.5722494203902513E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3180157.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.89151038618942
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55772904870376	45.08600295414812
2	12.92443982511154	15.222795643924725
3	4.432680689678425	7.831417826245746
4	2.0233114143527433	4.766234606914006
5	1.1206131761373046	3.299730125069539
6	0.6533222357707089	2.308507994005151
7	0.4681901942079068	1.9300699379434887
8	0.31615520162476923	1.4895085872106324
9	0.2359094268043228	1.2503756212962094
>10	1.1955691327385745	12.140821335145892
>50	0.05396101280402714	2.160525637299949
>100	0.016462002321099567	1.6262268506527362
>500	0.0011756798195445288	0.5169528022113776
>1k	4.8095992523102595E-4	0.37083007793249334
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	3.1444988407805024E-5
9	0.0	0.0	0.0	0.0	6.288997681561005E-5
10-11	0.0	0.0	0.0	0.0	9.433496522341507E-5
12-13	0.0	0.0	0.0	0.0	2.515599072624402E-4
14-15	0.0	0.0	0.0	0.0	3.7733986089366026E-4
16-17	0.0	0.0	0.0	0.0	4.402298377092703E-4
18-19	0.0	0.0	0.0	0.0	4.7167482611707535E-4
20-21	0.0	0.0	0.0	0.0	6.288997681561005E-4
22-23	0.0	1.5722494203902512E-5	0.0	0.0	6.288997681561005E-4
24-25	0.0	3.1444988407805024E-5	0.0	4.716748261170753E-5	6.288997681561005E-4
26-27	0.0	3.1444988407805024E-5	0.0	3.144498840780502E-4	6.603447565639055E-4
28-29	0.0	3.1444988407805024E-5	0.0	0.0012735220305161036	6.917897449717106E-4
30-31	0.0	3.1444988407805024E-5	0.0	0.006100327751114174	7.075122391756131E-4
32-33	0.0	3.1444988407805024E-5	0.0	0.02045496495927717	7.38957227583418E-4
34-35	3.1444988407805024E-5	3.1444988407805024E-5	0.0	0.04045397758664116	7.861247101951256E-4
36-37	3.1444988407805024E-5	4.716748261170753E-5	0.0	0.0763484318541506	7.861247101951256E-4
38-39	3.1444988407805024E-5	6.288997681561005E-5	0.0	0.13559079001445526	8.175696986029306E-4
40-41	3.1444988407805024E-5	6.288997681561005E-5	0.0	0.20211266299116679	8.175696986029306E-4
42-43	3.1444988407805024E-5	6.288997681561005E-5	0.0	0.27440469134071055	8.490146870107357E-4
44-45	3.1444988407805024E-5	6.288997681561005E-5	0.0	0.3649819804493929	8.961821696224432E-4
46-47	3.1444988407805024E-5	6.288997681561005E-5	0.0	0.47337285549109687	9.905171348458582E-4
48-49	3.1444988407805024E-5	6.288997681561005E-5	0.0	0.5865119237823793	0.0010376846174575659
50-51	3.1444988407805024E-5	6.288997681561005E-5	0.0	0.703660228095657	0.0010376846174575659
52-53	3.1444988407805024E-5	6.288997681561005E-5	0.0	0.8280251572485258	0.0010848521000692735
54-55	6.288997681561005E-5	6.288997681561005E-5	0.0	0.968474197971987	0.0011320195826809809
56-57	6.288997681561005E-5	6.288997681561005E-5	0.0	1.1349125216144988	0.0011477420768848834
58-59	6.288997681561005E-5	6.288997681561005E-5	0.0	1.310690006814129	0.001163464571088786
60-61	6.288997681561005E-5	6.288997681561005E-5	0.0	1.497599017910122	0.001163464571088786
62-63	6.288997681561005E-5	6.288997681561005E-5	0.0	1.6924793335674937	0.001257799536312201
64-65	6.288997681561005E-5	6.288997681561005E-5	0.0	1.9086636288711532	0.001257799536312201
66-67	6.288997681561005E-5	6.288997681561005E-5	0.0	2.159594636365437	0.001257799536312201
68-69	6.288997681561005E-5	6.288997681561005E-5	0.0	2.437363941465783	0.001257799536312201
70-71	6.288997681561005E-5	6.288997681561005E-5	0.0	2.722365593899924	0.0012735220305161036
72-73	6.288997681561005E-5	6.288997681561005E-5	0.0	3.024536210004726	0.0013364120073317135
74-75	6.288997681561005E-5	6.288997681561005E-5	0.0	3.343828622297578	0.0013993019841473235
76-77	6.288997681561005E-5	6.288997681561005E-5	0.0	3.7016725903784	0.001446469466759031
78-79	6.288997681561005E-5	7.861247101951255E-5	0.0	4.101762900385107	0.0014621919609629336
80-81	6.288997681561005E-5	9.433496522341507E-5	0.0	4.5228584626482276	0.001509359443574641
82-83	6.288997681561005E-5	9.433496522341507E-5	0.0	4.962003448257429	0.0016036944087980563
84-85	6.288997681561005E-5	9.433496522341507E-5	0.0	5.432593422274435	0.0016036944087980563
86-87	6.288997681561005E-5	9.433496522341507E-5	0.0	5.933339140174526	0.0016665843856136662
88-89	6.288997681561005E-5	9.433496522341507E-5	0.0	6.479821593713769	0.0017766418450409838
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2425	0.0	46.271088	1
GTATCAA	4215	0.0	39.81865	1
TCAACGC	5070	0.0	32.109726	4
CAACGCA	5265	0.0	30.855083	5
ATCAACG	5340	0.0	30.486681	3
AACGCAG	5665	0.0	28.668314	6
TATCAAC	5770	0.0	28.461485	2
GTACATG	7740	0.0	24.817032	1
ACGCAGA	6705	0.0	23.937956	7
TACATGG	8135	0.0	23.046068	2
ACATGGG	8320	0.0	22.533625	3
CGCAGAG	7145	0.0	22.065052	8
CATGGGG	6795	0.0	20.81504	4
AGAGTAC	6865	0.0	18.26191	10-11
GCAGAGT	8455	0.0	17.804445	9
ATGGGGG	3865	0.0	16.222712	5
ACTATAC	795	0.0	16.119587	3
CAGAGTA	7530	0.0	16.081553	10-11
TAAGGTG	1600	0.0	16.03145	5
TAATACT	2575	0.0	15.667227	4
>>END_MODULE