FastQCFastQC Report
Mon 15 Aug 2016
SRR2921688_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921688_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3208978
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24300.049.212921
GTATCAA41900.042.1321681
TCAACGC51450.033.3002364
ATCAACG52950.032.3563843
CAACGCA52750.032.2971085
TATCAAC55550.030.927872
AACGCAG56750.029.9207046
GTACATG91550.028.5094761
TACATGG95300.026.9918042
ACATGGG98200.025.7109933
ACGCAGA66950.024.5166667
CGCAGAG69600.023.2445778
CATGGGG85400.022.2849664
TAAGGCG8600.022.0725845
GTATTAA16700.020.743941
TATTAAG13700.019.7460292
GTATTAG7450.019.1095241
GCAGAGT81900.018.6535749
AGAGTAC66000.018.2953510-11
GAGTACA66200.017.9928761
Produced by FastQC (version 0.11.5)