##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921688_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3208978
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.105513032498198	31.0	31.0	34.0	26.0	34.0
2	30.16804945375132	31.0	31.0	34.0	26.0	34.0
3	30.228618270365207	31.0	31.0	34.0	26.0	34.0
4	33.67610435471979	37.0	35.0	37.0	28.0	37.0
5	33.66194096687481	37.0	35.0	37.0	28.0	37.0
6	33.697811577393175	37.0	35.0	37.0	28.0	37.0
7	33.60133600168029	37.0	35.0	37.0	28.0	37.0
8	33.57196621478863	37.0	35.0	37.0	28.0	37.0
9	35.10084861909306	39.0	35.0	39.0	28.0	39.0
10-11	34.99225797122947	39.0	35.0	39.0	27.5	39.0
12-13	34.875593880668546	39.0	35.0	39.0	27.0	39.0
14-15	35.9836430788868	40.0	36.0	41.0	27.0	41.0
16-17	35.913553941472955	40.0	36.0	41.0	27.0	41.0
18-19	35.87780050221597	40.0	36.0	41.0	27.0	41.0
20-21	35.7956593968547	40.0	36.0	41.0	27.0	41.0
22-23	35.69290331688157	40.0	36.0	41.0	26.5	41.0
24-25	35.57394192169595	40.0	36.0	41.0	26.0	41.0
26-27	35.42447221514139	40.0	36.0	41.0	25.0	41.0
28-29	35.2850215863119	40.0	35.5	41.0	24.5	41.0
30-31	35.13441522503426	39.0	35.0	41.0	24.0	41.0
32-33	34.957844366648814	39.0	35.0	41.0	24.0	41.0
34-35	34.78230078236747	39.0	35.0	41.0	23.0	41.0
36-37	34.74985166616911	39.0	35.0	41.0	22.5	41.0
38-39	34.93201714065974	39.0	35.0	41.0	23.5	41.0
40-41	34.99551368067964	40.0	35.0	41.0	23.0	41.0
42-43	34.83702583813289	40.0	35.0	41.0	22.0	41.0
44-45	34.710634039871884	39.5	35.0	41.0	20.5	41.0
46-47	34.55419949903053	39.0	35.0	41.0	19.5	41.0
48-49	34.38871145268057	39.0	34.5	41.0	17.5	41.0
50-51	32.69687124685804	37.0	32.0	39.5	14.0	40.0
52-53	32.811049343435826	37.5	32.5	39.5	13.0	40.0
54-55	33.71658484414664	38.0	33.5	40.0	13.0	41.0
56-57	33.72804347677048	38.0	34.0	41.0	12.5	41.0
58-59	33.53284269945135	38.0	33.5	40.5	9.0	41.0
60-61	33.272775163930696	37.5	33.0	40.0	8.0	41.0
62-63	32.977512310773086	37.0	33.0	40.0	6.5	41.0
64-65	32.683081342408705	36.5	33.0	40.0	2.0	41.0
66-67	32.33670891480091	36.0	32.5	39.0	2.0	41.0
68-69	31.969824660686363	35.5	32.0	39.0	2.0	41.0
70-71	31.564925188019366	35.0	32.0	38.5	2.0	40.0
72-73	31.204721253931936	35.0	32.0	37.0	2.0	39.5
74-75	30.80805368562826	35.0	31.0	37.0	2.0	39.0
76-77	30.44342108297408	35.0	31.0	36.0	2.0	39.0
78-79	30.108018191461582	35.0	31.0	36.0	2.0	37.5
80-81	29.807663062819376	35.0	31.0	35.5	2.0	37.0
82-83	29.50952593006247	35.0	30.0	35.0	2.0	37.0
84-85	29.261150278998485	35.0	30.0	35.0	2.0	36.0
86-87	29.0391255409043	34.5	30.0	35.0	2.0	36.0
88-89	28.82433986770866	34.0	30.0	35.0	2.0	36.0
90-91	28.609192552893788	34.0	30.0	35.0	2.0	35.0
92-93	28.424723852890235	34.0	29.0	35.0	2.0	35.0
94-95	28.2033106802228	34.0	29.0	35.0	2.0	35.0
96-97	27.971507283627375	34.0	29.0	35.0	2.0	35.0
98-99	27.70593005000346	34.0	29.0	35.0	2.0	35.0
100-101	26.63210062518347	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	127459.0
3	21298.0
4	14290.0
5	11006.0
6	9955.0
7	10131.0
8	10770.0
9	11370.0
10	12228.0
11	12893.0
12	13033.0
13	13027.0
14	13756.0
15	14102.0
16	14068.0
17	14900.0
18	14313.0
19	13812.0
20	14100.0
21	14765.0
22	16259.0
23	18813.0
24	21588.0
25	25364.0
26	30362.0
27	35926.0
28	42513.0
29	49582.0
30	59513.0
31	72186.0
32	90216.0
33	116217.0
34	159720.0
35	231624.0
36	384144.0
37	680343.0
38	685007.0
39	108318.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.030288886207465	17.904316128869322	12.979378900733932	25.086016084189282
2	16.0398688917234	19.420834489834743	39.08900954620444	25.450287072237415
3	18.777632073397584	23.83703216289923	29.239438728016353	28.145897035686833
4	13.388285990409274	15.742776159087443	36.14466736043565	34.72427049006764
5	14.578644668963275	36.92631719440095	34.18794490013126	14.307093236504521
6	33.94040049852726	34.838441450939975	17.147642420443983	14.073515630088782
7	29.23165389596122	31.293480005547735	21.281994941585126	18.19287115690592
8	26.88617144224979	34.373819506520995	19.98869219711732	18.751316854111888
9	27.081964045581113	15.230687004582732	19.171555588797627	38.515793361038526
10-11	25.8522707793499	24.838057487721855	27.90818644956314	21.401485283365112
12-13	26.369479899520197	22.353249026637	27.59535154682974	23.681919527013065
14-15	23.703622532254435	23.88015311475548	24.626444920353922	27.789779432636163
16-17	24.035606643655825	26.72128546504687	25.46656803986717	23.776539851430133
18-19	24.03245564183168	26.31615936481213	26.46285769404999	23.188527299306198
20-21	24.60677092099636	25.558752463915557	26.238176010532655	23.596300604555424
22-23	24.490295665923256	25.320225255023626	26.00608153648267	24.183397542570443
24-25	24.29300587379661	25.497271343715923	25.99851736669861	24.21120541578886
26-27	24.08315097990842	25.669014079019487	26.000764583066122	24.247070358005967
28-29	24.25873041152434	25.78134958640621	25.853499815962373	24.106420186107076
30-31	24.5399763447127	25.588024004350828	25.668028118313835	24.20397153262264
32-33	24.29643965742922	25.695130084645402	25.558547440068814	24.449882817856565
34-35	24.602794942402074	25.659234803334336	25.63452148407066	24.10344877019293
36-37	24.68543073960451	25.442640688087682	25.70213354735963	24.16979502494818
38-39	24.4495144876172	25.60749233403551	25.654814930006342	24.288178248340945
40-41	24.63086639702356	25.46413402835148	25.732992867099437	24.172006707525522
42-43	24.474117886122354	25.428926784552058	25.580056831520142	24.516898497805446
44-45	24.3474825827767	25.469913717995556	25.741328861533464	24.441274837694273
46-47	24.552903544651873	25.452975451093863	25.56093661068478	24.433184393569487
48-49	24.272067833781747	25.56785947021177	25.59906041129961	24.56101228470687
50-51	24.29798669916379	25.570889622046828	25.758358617288902	24.37276506150048
52-53	24.575544915939748	25.42159737903163	25.685052797271595	24.31780490775703
54-55	24.148693637141555	25.325450809176708	25.924447692394175	24.601407861287562
56-57	24.222579509747796	25.473366932102564	25.79636235076453	24.507691207385108
58-59	24.343325874534763	25.27778402467775	25.80191454990461	24.576975550882874
60-61	24.276282591003202	25.352079900307345	25.93870663493719	24.43293087375226
62-63	24.313433635236343	25.342925980018844	25.94863187981511	24.395008504929706
64-65	24.141101501906096	25.240723312204118	25.861920080625506	24.756255105264284
66-67	24.178419004277874	25.285831799423995	25.768693134228048	24.767056062070083
68-69	24.162651260832227	25.269700991490357	25.852322585681943	24.715325161995473
70-71	24.12052008686329	25.084609587468055	25.845654813492008	24.949215512176647
72-73	24.05915302809733	25.237910166183052	25.771747085468714	24.931189720250906
74-75	24.06335524007502	25.322890090969008	25.80061830632036	24.81313636263561
76-77	24.114868319873217	25.002181982832987	25.926749392050404	24.956200305243396
78-79	24.0075222032897	25.059628078207304	25.958332103526182	24.97451761497682
80-81	24.110644033489024	25.165168729730215	25.79301372312753	24.931173513653235
82-83	24.08678626795683	25.173012461270357	25.892831763010648	24.84736950776217
84-85	24.019580436180703	25.16583306341309	25.894492783680974	24.920093716725237
86-87	23.975798917068396	25.203660841804915	25.89454015083813	24.926000090288557
88-89	23.849060387809384	24.984782738744656	25.974697408059548	25.191459465386412
90-91	23.859629665499284	24.988550059514697	26.03297832790255	25.11884194708347
92-93	23.990223851129127	24.80278383133566	26.1451533493947	25.061838968140513
94-95	23.839758245901944	24.73782298535258	26.183008514534095	25.239410254211382
96-97	23.844995438469326	24.57999550095605	26.238080657860202	25.336928402714427
98-99	23.702119437205642	24.556092536506295	26.13466969891942	25.607118327368646
100-101	23.82399167429393	24.479381708222554	26.25274960285901	25.443877014624505
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	50.0
1	44.5
2	65.0
3	84.0
4	74.5
5	83.0
6	137.5
7	303.5
8	475.5
9	517.5
10	465.5
11	436.0
12	478.5
13	552.5
14	661.5
15	761.0
16	852.5
17	949.0
18	1053.0
19	1129.0
20	1234.5
21	1379.5
22	1472.0
23	1645.5
24	1764.5
25	1984.0
26	2481.5
27	3127.5
28	4103.0
29	5652.0
30	8391.5
31	12393.0
32	18085.0
33	23968.5
34	29960.5
35	37528.5
36	46889.5
37	56877.5
38	66546.5
39	75840.5
40	83826.5
41	91553.5
42	100023.0
43	108860.5
44	116960.0
45	125106.0
46	131080.0
47	135577.5
48	140945.5
49	144167.5
50	144049.5
51	143544.0
52	143299.5
53	142456.0
54	140671.0
55	134758.5
56	125279.0
57	113467.0
58	102402.0
59	89946.5
60	74351.0
61	61515.0
62	50880.0
63	39291.5
64	28653.0
65	21034.5
66	15842.0
67	12384.5
68	9373.5
69	7062.0
70	5404.5
71	3901.0
72	2833.5
73	2132.5
74	1600.5
75	1154.5
76	812.0
77	553.0
78	368.0
79	250.5
80	190.0
81	162.0
82	125.0
83	99.0
84	84.0
85	68.0
86	56.0
87	47.5
88	51.5
89	44.5
90	32.0
91	31.0
92	27.0
93	24.0
94	21.5
95	17.5
96	15.5
97	12.5
98	9.0
99	7.5
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05388008269299446
2	0.076909221565246
3	0.07270227468059924
4	0.07288925009769465
5	0.06914974175578642
6	0.010190160231699935
7	0.0144282696858626
8	0.017887314902127718
9	0.006014375916569076
10-11	0.01346223003086964
12-13	0.0240263410967604
14-15	0.04108784790671672
16-17	0.0288253768022093
18-19	0.010984805754355436
20-21	0.020878921575654304
22-23	0.03851693592165481
24-25	0.036522531472637086
26-27	0.021611241959278
28-29	0.01230921495878127
30-31	0.012246889819749467
32-33	0.00928644571573878
34-35	0.005671587651894155
36-37	8.258080921714016E-4
38-39	0.004721129281659145
40-41	0.0014023156282155877
42-43	0.241868283297673
44-45	0.20841526492235224
46-47	0.2150373109444814
48-49	0.22285911589297278
50-51	0.21341685732965449
52-53	0.22150354411903106
54-55	0.21682915869164573
56-57	0.2204751793250063
58-59	0.23029138872251537
60-61	0.23491903029562683
62-63	0.21849635616074653
64-65	0.2185586812997783
66-67	0.21564498105004146
68-69	0.2096617677029883
70-71	0.20176205633070715
72-73	0.21428940927609974
74-75	0.21326104448207497
76-77	0.20615597863244936
78-79	0.22613118569214247
80-81	0.2377859866910898
82-83	0.23742761714165694
84-85	0.23107045296041295
86-87	0.2533361088795249
88-89	0.24442361399797694
90-91	0.252042862244615
92-93	0.24686987570497523
94-95	0.2505314776230937
96-97	0.25858700184295436
98-99	0.2510924038743799
100-101	0.25829095743255326
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3208978.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.478052186475246
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.37949290512915	45.83480076455609
2	11.461292408718654	13.40468111203004
3	3.998815801409118	7.0152887735671285
4	1.8796518534453104	4.396735167127192
5	1.0937975568605338	3.198157530576471
6	0.6777315258394917	2.377945172187678
7	0.46524186525852196	1.9044506653144655
8	0.34628204275502633	1.6199919493974146
9	0.2484380690146234	1.3075356928449857
>10	1.3671647170953363	14.14227431081781
>50	0.06352482978338567	2.4886965405935415
>100	0.017335371518029666	1.6261671966981643
>500	8.563848161987984E-4	0.37857135280090687
>1k	3.7466835661051215E-4	0.304703771488035
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.1162569515901944E-5
2	0.0	0.0	0.0	0.0	3.1162569515901944E-5
3	0.0	0.0	0.0	0.0	3.1162569515901944E-5
4	0.0	0.0	0.0	0.0	3.1162569515901944E-5
5	0.0	0.0	0.0	0.0	3.1162569515901944E-5
6	0.0	0.0	0.0	0.0	9.348770854770584E-5
7	0.0	0.0	0.0	0.0	9.348770854770584E-5
8	0.0	0.0	0.0	0.0	1.5581284757950974E-4
9	0.0	0.0	0.0	0.0	2.1813798661131365E-4
10-11	0.0	0.0	0.0	0.0	2.1813798661131365E-4
12-13	0.0	0.0	0.0	0.0	2.4930055612721556E-4
14-15	0.0	0.0	0.0	0.0	2.9604441040106846E-4
16-17	0.0	0.0	0.0	3.1162569515901944E-5	3.583695494328724E-4
18-19	0.0	0.0	0.0	3.1162569515901944E-5	4.362759732226273E-4
20-21	0.0	0.0	0.0	3.1162569515901944E-5	4.6743854273852923E-4
22-23	0.0	0.0	0.0	6.232513903180389E-5	5.453449665282841E-4
24-25	0.0	0.0	0.0	1.713941323374607E-4	6.855765293498428E-4
26-27	0.0	0.0	0.0	5.141823970123821E-4	7.790642378975487E-4
28-29	0.0	0.0	0.0	0.0021190547270813326	7.946455226554997E-4
30-31	0.0	0.0	0.0	0.009411095993802389	8.102268074134506E-4
32-33	0.0	0.0	0.0	0.02944862819252734	9.348770854770585E-4
34-35	0.0	0.0	0.0	0.05509542290411464	0.0010283647940247643
36-37	0.0	0.0	0.0	0.09886325178919893	0.0010283647940247643
38-39	0.0	4.674385427385292E-5	0.0	0.17463503956711451	0.0010595273635406663
40-41	0.0	6.232513903180389E-5	0.0	0.2604255934443926	0.0010751086482986172
42-43	0.0	6.232513903180389E-5	0.0	0.34990891180930506	0.0010906899330565681
44-45	0.0	6.232513903180389E-5	0.0	0.4584169788636756	0.0011218525025724702
46-47	0.0	6.232513903180389E-5	0.0	0.5888479135724832	0.001215340211120176
48-49	0.0	6.232513903180389E-5	0.0	0.7224262678023969	0.0012776653501519798
50-51	0.0	6.232513903180389E-5	0.0	0.8670984967799718	0.0013088279196678818
52-53	0.0	7.790642378975487E-5	0.0	1.0155258153842128	0.0014646407672473916
54-55	0.0	9.348770854770584E-5	0.0	1.180110926282449	0.0016671974691007542
56-57	0.0	1.0906899330565681E-4	0.0	1.3790527700719668	0.001682778753858705
58-59	0.0	1.5581284757950974E-4	0.0	1.5899454592708333	0.0016983600386166562
60-61	0.0	1.5581284757950974E-4	0.0	1.814721073189034	0.0017918477471643621
62-63	0.0	1.5581284757950974E-4	0.0	2.041880623675201	0.0019320793099859208
64-65	0.0	1.5581284757950974E-4	0.0	2.2878467848642154	0.0019476605947438717
66-67	0.0	1.5581284757950974E-4	0.0	2.5713482610351335	0.0019788231642597735
68-69	0.0	1.5581284757950974E-4	0.0	2.888333918150888	0.0019944044490177244
70-71	0.0	1.713941323374607E-4	0.0	3.2200750519324224	0.0020567295880495285
72-73	0.0	1.8697541709541168E-4	0.0	3.561102631429695	0.0021346360118392835
74-75	0.0	1.8697541709541168E-4	0.0	3.924785398965029	0.0021657985813551854
76-77	0.0	1.8697541709541168E-4	0.0	4.330771354618199	0.002212542435629038
78-79	0.0	1.8697541709541168E-4	0.0	4.7781567838732455	0.002212542435629038
80-81	0.0	1.8697541709541168E-4	0.0	5.247106711233296	0.002259286289902891
82-83	0.0	1.8697541709541168E-4	0.0	5.737667880552625	0.002383936567966499
84-85	0.0	1.8697541709541168E-4	0.0	6.242594994418784	0.00239951785272445
86-87	0.0	1.8697541709541168E-4	0.0	6.789046232164882	0.00239951785272445
88-89	0.0	1.8697541709541168E-4	0.0	7.375556953023674	0.00239951785272445
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2430	0.0	49.21292	1
GTATCAA	4190	0.0	42.132168	1
TCAACGC	5145	0.0	33.300236	4
ATCAACG	5295	0.0	32.356384	3
CAACGCA	5275	0.0	32.297108	5
TATCAAC	5555	0.0	30.92787	2
AACGCAG	5675	0.0	29.920704	6
GTACATG	9155	0.0	28.509476	1
TACATGG	9530	0.0	26.991804	2
ACATGGG	9820	0.0	25.710993	3
ACGCAGA	6695	0.0	24.516666	7
CGCAGAG	6960	0.0	23.244577	8
CATGGGG	8540	0.0	22.284966	4
TAAGGCG	860	0.0	22.072584	5
GTATTAA	1670	0.0	20.74394	1
TATTAAG	1370	0.0	19.746029	2
GTATTAG	745	0.0	19.109524	1
GCAGAGT	8190	0.0	18.653574	9
AGAGTAC	6600	0.0	18.29535	10-11
GAGTACA	6620	0.0	17.992876	1
>>END_MODULE