FastQCFastQC Report
Mon 15 Aug 2016
SRR2921688_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921688_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3208978
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.043.686651
GTATCAA40250.042.9822431
TCAACGC49700.033.9941064
CAACGCA52100.032.454975
ATCAACG53300.031.6080463
TATCAAC53550.031.54912
AACGCAG54600.031.0510836
GTACATG89850.027.8241061
TACATGG93500.026.0884322
ACATGGG96950.024.6705723
ACGCAGA67750.024.5323757
CATGGGG82600.022.2926484
CGCAGAG73800.022.199528
TAAGGTG16300.020.6890095
TAAGGCG8500.020.6752975
TATACCG3102.6011548E-1019.9182225
ATGGGGG46850.019.3638955
GCAGAGT84250.018.7132429
AGAGTAC70100.018.25667210-11
GTATAGC7900.018.0487751
Produced by FastQC (version 0.11.5)