##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921688_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3208978
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.300243878269033	33.0	31.0	34.0	28.0	34.0
2	31.43145356559004	34.0	31.0	34.0	28.0	34.0
3	31.504270206900763	34.0	31.0	34.0	28.0	34.0
4	34.98583598890363	37.0	35.0	37.0	32.0	37.0
5	34.72236363103767	37.0	35.0	37.0	32.0	37.0
6	34.69266819529458	37.0	35.0	37.0	32.0	37.0
7	34.57197618681088	37.0	35.0	37.0	32.0	37.0
8	34.51607832774173	37.0	35.0	37.0	31.0	37.0
9	36.09568248831871	39.0	37.0	39.0	31.0	39.0
10-11	35.98302855301594	39.0	37.0	39.0	31.0	39.0
12-13	35.90780678458998	39.0	37.0	39.0	30.5	39.0
14-15	37.13719305648091	40.0	38.0	41.0	31.0	41.0
16-17	37.09710786424837	40.0	38.0	41.0	31.0	41.0
18-19	37.055408762540594	40.0	38.0	41.0	31.0	41.0
20-21	36.99509937431793	40.0	37.5	41.0	31.0	41.0
22-23	36.904636616393134	40.0	37.0	41.0	30.0	41.0
24-25	36.815154544531	40.0	37.0	41.0	30.0	41.0
26-27	36.68702403070385	40.0	37.0	41.0	30.0	41.0
28-29	36.56461636695546	40.0	37.0	41.0	30.0	41.0
30-31	36.411792633043916	40.0	37.0	41.0	29.5	41.0
32-33	36.36346774580568	40.0	36.5	41.0	29.5	41.0
34-35	36.50115426157487	40.0	37.0	41.0	30.0	41.0
36-37	36.49647816220616	40.0	37.0	41.0	30.0	41.0
38-39	36.39232973862707	40.0	37.0	41.0	30.0	41.0
40-41	36.31068598787527	40.0	37.0	41.0	29.0	41.0
42-43	36.191106171497594	40.0	36.0	41.0	29.0	41.0
44-45	36.060818117170015	40.0	36.0	41.0	28.0	41.0
46-47	35.90263239573471	40.0	36.0	41.0	28.0	41.0
48-49	35.762286933721576	40.0	35.0	41.0	27.5	41.0
50-51	35.599880553871046	39.5	35.0	41.0	27.0	41.0
52-53	35.420497741025336	39.0	35.0	41.0	26.5	41.0
54-55	35.199077089341216	39.0	35.0	41.0	26.0	41.0
56-57	34.96508109435465	39.0	35.0	41.0	26.0	41.0
58-59	34.713192175203446	38.0	35.0	40.0	26.0	41.0
60-61	34.427181021496565	38.0	34.0	40.0	25.0	41.0
62-63	34.114431604080806	37.0	34.0	40.0	24.5	41.0
64-65	33.771655648620836	37.0	34.0	40.0	23.5	41.0
66-67	33.417270856951966	36.0	34.0	39.0	23.0	41.0
68-69	33.050056902851935	36.0	33.0	39.0	22.0	40.5
70-71	32.65246022253814	35.0	33.0	38.5	21.5	40.0
72-73	32.293377829327596	35.0	33.0	37.0	20.5	39.5
74-75	31.892786270270474	35.0	32.5	37.0	19.5	39.0
76-77	29.201578041357713	33.0	28.5	34.5	13.0	36.5
78-79	30.770824698704697	34.5	31.5	35.5	15.0	37.0
80-81	30.960189194192044	35.0	32.0	35.0	17.0	37.0
82-83	30.793926602176768	35.0	32.0	35.0	15.0	37.0
84-85	30.60610699107317	35.0	32.0	35.0	12.0	36.0
86-87	30.40825396746254	35.0	32.0	35.0	7.5	36.0
88-89	30.146397856264517	35.0	31.5	35.0	2.0	35.5
90-91	29.950602185493324	34.0	31.0	35.0	2.0	35.0
92-93	29.795101119421822	34.0	31.0	35.0	2.0	35.0
94-95	29.687608328882277	34.0	31.0	35.0	2.0	35.0
96-97	29.484365583061024	34.0	31.0	35.0	2.0	35.0
98-99	29.280530904231817	34.0	31.0	35.0	2.0	35.0
100-101	28.155399164469188	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65181.0
3	21139.0
4	11617.0
5	8377.0
6	7069.0
7	7031.0
8	7555.0
9	8207.0
10	9061.0
11	9594.0
12	10175.0
13	10575.0
14	11602.0
15	12255.0
16	13047.0
17	13712.0
18	12967.0
19	12144.0
20	11628.0
21	11628.0
22	12365.0
23	13986.0
24	16793.0
25	20345.0
26	23900.0
27	29347.0
28	34907.0
29	42431.0
30	51751.0
31	64313.0
32	81437.0
33	107765.0
34	155165.0
35	231036.0
36	407708.0
37	780158.0
38	774727.0
39	86280.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.7939633959238	19.42648765125145	13.574461616164454	26.20508733666029
2	15.115962370503699	20.8072554076508	37.4131030124289	26.663679209416596
3	17.430243962642198	24.981339486705767	29.01780719412263	28.570609356529403
4	13.081429956026764	16.908098741885517	35.10008507932946	34.910386222758255
5	14.381617742633809	37.27038465003209	34.14246336736866	14.205534239965436
6	33.962479942484904	35.36274302353737	16.84338886539518	13.831388168582537
7	29.066575090262383	31.561294592857912	21.228253979927565	18.14387633695214
8	26.909782491497293	34.74349154154376	19.7822795918202	18.56444637513875
9	26.927732131538452	15.196925625541837	19.03964439768674	38.83569784523297
10-11	25.780619120101917	25.104121002978808	27.808694844240968	21.30656503267831
12-13	26.264763895314026	22.560115501289587	27.570043146263355	23.60507745713303
14-15	23.60812758564272	24.021098065491262	24.591852157721284	27.778922191144733
16-17	23.988057880110116	26.95574728153325	25.355767474878295	23.70042736347834
18-19	24.014082027153744	26.46341611629857	26.3967268666991	23.125774989848587
20-21	24.59606235390575	25.660982501255074	26.225524490234104	23.51743065460507
22-23	24.4221992173209	25.403742250648026	25.985313704238543	24.188744827792526
24-25	24.279036763854634	25.645525870038316	25.88337599781362	24.192061368293434
26-27	24.056943339003027	25.82960564983231	25.95176688146683	24.161684129697832
28-29	24.19116254971699	25.901076341141323	25.87295045246842	24.034810656673276
30-31	24.43947278525674	25.71008737043418	25.65008876676532	24.200351077543754
32-33	24.253649822148795	25.775724533680155	25.51656012424184	24.454065519929216
34-35	24.478136084319758	25.827304564577613	25.644797504739543	24.049761846363086
36-37	24.559020021986086	25.62130172904758	25.70824883388818	24.111429415078156
38-39	24.444636267372353	25.721631622279737	25.637648497434384	24.19608361291352
40-41	24.548654783704578	25.57280610012553	25.717452846064575	24.161086270105322
42-43	24.387080970198795	25.544292293797227	25.57336983452916	24.49525690147482
44-45	24.31086778169851	25.550175264181828	25.70155926297886	24.437397691140802
46-47	24.48319045488776	25.611944977735767	25.52833237738234	24.37653218999413
48-49	24.19947286521163	25.678934585608292	25.623304007552044	24.498288541628035
50-51	24.275136044436714	25.663019909139578	25.680518237215626	24.38132580920808
52-53	24.53212527600982	25.488668463648818	25.597568760744537	24.381637499596824
54-55	24.069655142969243	25.480618055700553	25.79102646846817	24.65870033286203
56-57	24.186316054213705	25.551802921361833	25.677086953039368	24.584794071385097
58-59	24.23901140557608	25.41994645742081	25.71920082269627	24.62184131430685
60-61	24.217153285808937	25.515498616590072	25.823657151191526	24.44369094640946
62-63	24.149719022411816	25.542185911815835	25.794892750376135	24.513202315396214
64-65	24.018544220690067	25.33123586986748	25.724089405315485	24.926130504126963
66-67	24.02920995818975	25.50347467725562	25.58395516541453	24.883360199140096
68-69	24.078168849628927	25.44123025349051	25.673993300988762	24.806607595891798
70-71	23.987448253280217	25.30903024113387	25.65407074195636	25.04945076362956
72-73	23.991110056767763	25.51318317980844	25.488353809640564	25.007352953783236
74-75	24.014121846750033	25.558924826975893	25.484721542081722	24.942231784192348
76-77	24.007338448861688	25.214540461422075	25.65794282294454	25.120178266771703
78-79	23.91734117684853	25.278728145378874	25.718343012189916	25.085587665582675
80-81	24.09346532899751	25.3706063293555	25.490727123724415	25.045201217922568
82-83	24.035783958999847	25.385716808358133	25.54529796665332	25.0332012659887
84-85	23.9311694432012	25.37227668348598	25.56307428104818	25.133479592264646
86-87	23.907472087781116	25.44982224461218	25.49034557717995	25.15236009042675
88-89	23.76647612963436	25.2446044806884	25.56511800242096	25.423801387256283
90-91	23.698946079358326	25.29068849751531	25.611890652803176	25.398474770323194
92-93	23.805057032033254	25.082106266796767	25.72144933559673	25.391387365573255
94-95	23.664834071467006	25.02153939555057	25.725806338511564	25.58782019447086
96-97	23.615469114583732	24.949801254263484	25.76593117679885	25.66879845435393
98-99	23.454070871913004	24.865703483762882	25.67314979631929	26.007075848004828
100-101	23.419570765697404	24.907384612508064	25.714949033490498	25.958095588304037
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	52.0
1	44.5
2	54.0
3	68.0
4	60.5
5	72.0
6	111.0
7	258.5
8	395.5
9	422.5
10	370.5
11	302.5
12	307.5
13	331.5
14	357.5
15	411.5
16	472.5
17	491.0
18	561.5
19	657.0
20	702.5
21	802.5
22	914.5
23	1104.0
24	1359.0
25	1675.0
26	2182.0
27	2940.5
28	4106.5
29	5793.0
30	8645.0
31	12859.0
32	18747.5
33	24588.0
34	30398.0
35	37690.5
36	47256.5
37	57267.5
38	66397.0
39	75502.0
40	83490.5
41	91313.0
42	99872.0
43	108843.0
44	117010.5
45	124921.0
46	131159.5
47	136082.0
48	141900.0
49	145398.0
50	145402.5
51	144676.0
52	144218.0
53	144355.0
54	143106.5
55	136638.0
56	126638.5
57	114508.5
58	103003.0
59	89973.0
60	73979.5
61	61091.0
62	50365.0
63	38576.0
64	27606.0
65	19958.0
66	14888.0
67	11572.0
68	8797.0
69	6561.0
70	4924.0
71	3501.5
72	2443.5
73	1793.0
74	1338.5
75	899.0
76	609.5
77	359.0
78	193.0
79	111.5
80	60.5
81	37.0
82	32.5
83	21.5
84	12.5
85	10.5
86	5.5
87	2.5
88	2.0
89	4.0
90	3.5
91	0.5
92	0.5
93	1.0
94	1.0
95	1.0
96	0.5
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16999181670924512
2	1.8697541709541168E-4
3	0.010314810509763545
4	0.006481814459307606
5	0.026207720962873538
6	0.0025241681307880577
7	0.0
8	0.0
9	0.0
10-11	0.05148056484027001
12-13	2.9604441040106846E-4
14-15	0.004767873135932998
16-17	0.0
18-19	0.0020255670185336267
20-21	1.5581284757950974E-4
22-23	0.0
24-25	7.634829531395976E-4
26-27	0.003240907229653803
28-29	0.005936469492779321
30-31	0.01776266462406411
32-33	0.012371540097813074
34-35	0.010439460787827152
36-37	0.004066715321825204
38-39	0.0
40-41	0.003926483759003645
42-43	0.009847371967025016
44-45	0.007806223663733437
46-47	0.004097877891341106
48-49	0.009691559119445506
50-51	0.0031162569515901946
52-53	0.002477424276514205
54-55	0.004393922301742174
56-57	0.008382731199777624
58-59	0.01569035375125663
60-61	0.007479016683816467
62-63	0.022281237203869892
64-65	0.04032436495357712
66-67	0.061997932051886924
68-69	0.05107545143656329
70-71	0.0702092691193271
72-73	0.033764644070479764
74-75	0.02801514999479585
76-77	0.23322067025701018
78-79	0.08608659828767913
80-81	0.0687446283520797
82-83	0.02781259329294249
84-85	0.05149614612502797
86-87	0.0680434705379719
88-89	0.29370721768737584
90-91	0.29888020422701556
92-93	0.05302311203130716
94-95	0.010049928668878378
96-97	0.015004777221906787
98-99	0.0010595273635406663
100-101	2.4930055612721556E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3208978.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.17433816011831
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.2331987764383	44.15757024031719
2	12.171754858174683	13.918240565266842
3	4.154770012478387	7.126386770128747
4	1.9354285955990238	4.426273960381658
5	1.10763319148328	3.1664097323618066
6	0.7168593275193192	2.459157456289474
7	0.4969117424590349	1.9887419999360536
8	0.3508166500337137	1.604616782498194
9	0.2765092163835424	1.422830829771178
>10	1.4686806331593503	14.789390285198827
>50	0.06883097011256395	2.6448766147825253
>100	0.017131009977430067	1.5616196131747921
>500	0.0010926045791579944	0.43743877802546816
>1k	3.824116022079579E-4	0.2964463718672288
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.1162569515901944E-5
5	0.0	3.1162569515901944E-5	0.0	0.0	3.1162569515901944E-5
6	0.0	3.1162569515901944E-5	0.0	0.0	3.1162569515901944E-5
7	0.0	3.1162569515901944E-5	0.0	0.0	3.1162569515901944E-5
8	0.0	3.1162569515901944E-5	0.0	0.0	3.1162569515901944E-5
9	0.0	3.1162569515901944E-5	0.0	0.0	6.232513903180389E-5
10-11	0.0	3.1162569515901944E-5	0.0	0.0	6.232513903180389E-5
12-13	0.0	4.674385427385292E-5	0.0	0.0	1.5581284757950974E-4
14-15	0.0	6.232513903180389E-5	0.0	0.0	2.1813798661131362E-4
16-17	0.0	6.232513903180389E-5	0.0	3.1162569515901944E-5	2.4930055612721556E-4
18-19	0.0	9.348770854770584E-5	0.0	3.1162569515901944E-5	3.116256951590195E-4
20-21	0.0	9.348770854770584E-5	0.0	3.1162569515901944E-5	3.116256951590195E-4
22-23	0.0	9.348770854770584E-5	0.0	9.348770854770584E-5	3.583695494328724E-4
24-25	0.0	9.348770854770584E-5	0.0	2.0255670185336265E-4	4.5185725798057826E-4
26-27	0.0	9.348770854770584E-5	0.0	6.07670105560088E-4	4.830198274964802E-4
28-29	0.0	9.348770854770584E-5	0.0	0.0023683552832085477	4.986011122544311E-4
30-31	0.0	9.348770854770584E-5	0.0	0.009894115821298868	4.986011122544311E-4
32-33	0.0	9.348770854770584E-5	0.0	0.030414667847520303	4.986011122544311E-4
34-35	0.0	9.348770854770584E-5	0.0	0.05680936422748925	5.297636817703332E-4
36-37	0.0	1.0906899330565681E-4	0.0	0.10173020818466191	5.453449665282842E-4
38-39	0.0	1.2465027806360778E-4	0.0	0.17818757249192735	5.92088820802137E-4
40-41	0.0	1.2465027806360778E-4	0.0	0.2653336981431471	5.92088820802137E-4
42-43	0.0	1.2465027806360778E-4	0.0	0.35568956845450483	6.076701055600881E-4
44-45	0.0	1.2465027806360778E-4	0.0	0.4655064634285433	6.699952445918919E-4
46-47	0.0	1.2465027806360778E-4	0.0	0.5977448271692731	7.167390988657448E-4
48-49	0.0	1.2465027806360778E-4	0.0	0.7326943344578867	7.479016683816467E-4
50-51	0.0	1.2465027806360778E-4	0.0	0.8788467854874666	7.790642378975487E-4
52-53	0.0	1.2465027806360778E-4	0.0	1.0283647940247644	9.660396549929604E-4
54-55	0.0	1.2465027806360778E-4	0.0	1.1949443093720182	0.0011218525025724702
56-57	0.0	1.2465027806360778E-4	0.0	1.3944159168433066	0.0011218525025724702
58-59	0.0	1.8697541709541168E-4	1.5581284757950972E-5	1.6054332563202365	0.0011218525025724702
60-61	0.0	1.8697541709541168E-4	3.1162569515901944E-5	1.8313774665952836	0.001153015072088372
62-63	0.0	1.8697541709541168E-4	3.1162569515901944E-5	2.060266539689583	0.001215340211120176
64-65	0.0	1.8697541709541168E-4	3.1162569515901944E-5	2.30727664695738	0.001215340211120176
66-67	0.0	1.8697541709541168E-4	3.1162569515901944E-5	2.592320670319335	0.001246502780636078
68-69	0.0	1.8697541709541168E-4	3.1162569515901944E-5	2.910957943619433	0.0012776653501519798
70-71	0.0	1.8697541709541168E-4	3.1162569515901944E-5	3.2444130187243414	0.0014490594824894407
72-73	0.0	1.8697541709541168E-4	3.1162569515901944E-5	3.5882763920475615	0.0016671974691007542
74-75	0.0	1.8697541709541168E-4	3.1162569515901944E-5	3.955636342785772	0.001682778753858705
76-77	3.1162569515901944E-5	1.8697541709541168E-4	3.1162569515901944E-5	4.366514821852939	0.001682778753858705
78-79	3.1162569515901944E-5	1.8697541709541168E-4	3.1162569515901944E-5	4.8186525429591605	0.0017139413233746071
80-81	3.1162569515901944E-5	1.8697541709541168E-4	3.1162569515901944E-5	5.293055919984493	0.001729522608132558
82-83	3.1162569515901944E-5	1.8697541709541168E-4	3.1162569515901944E-5	5.790270297895467	0.0017451038928905092
84-85	3.1162569515901944E-5	1.8697541709541168E-4	3.1162569515901944E-5	6.303922931226079	0.0017451038928905092
86-87	3.1162569515901944E-5	1.8697541709541168E-4	3.1162569515901944E-5	6.8575415599608345	0.0017918477471643621
88-89	3.1162569515901944E-5	2.0255670185336265E-4	3.1162569515901944E-5	7.450798977119819	0.0018385916014382149
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2165	0.0	43.68665	1
GTATCAA	4025	0.0	42.982243	1
TCAACGC	4970	0.0	33.994106	4
CAACGCA	5210	0.0	32.45497	5
ATCAACG	5330	0.0	31.608046	3
TATCAAC	5355	0.0	31.5491	2
AACGCAG	5460	0.0	31.051083	6
GTACATG	8985	0.0	27.824106	1
TACATGG	9350	0.0	26.088432	2
ACATGGG	9695	0.0	24.670572	3
ACGCAGA	6775	0.0	24.532375	7
CATGGGG	8260	0.0	22.292648	4
CGCAGAG	7380	0.0	22.19952	8
TAAGGTG	1630	0.0	20.689009	5
TAAGGCG	850	0.0	20.675297	5
TATACCG	310	2.6011548E-10	19.918222	5
ATGGGGG	4685	0.0	19.363895	5
GCAGAGT	8425	0.0	18.713242	9
AGAGTAC	7010	0.0	18.256672	10-11
GTATAGC	790	0.0	18.048775	1
>>END_MODULE