FastQCFastQC Report
Mon 15 Aug 2016
SRR2921687_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921687_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3753793
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA49900.045.2712751
GGTATCA26700.042.6595541
TCAACGC59200.037.43944
CAACGCA61650.035.8673825
ATCAACG63600.034.9238783
TATCAAC64500.034.510612
AACGCAG67650.032.8099026
GTACATG109750.029.1455141
ACGCAGA76950.028.2943957
TACATGG115250.027.7561022
CGCAGAG80300.026.4079468
ACATGGG118800.025.7677783
CATGGGG104400.023.8212474
GCAGAGT95550.020.258219
ATGGGGG60300.019.8303365
TATACCG3850.019.7199615
AGAGTAC76000.018.94659610-11
CGTATAC2755.9667036E-717.2584193
GAGTACA80600.016.9579661
CAGAGTA85950.016.47724210-11
Produced by FastQC (version 0.11.5)