##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921687_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3753793
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.320284842557914	31.0	31.0	34.0	26.0	34.0
2	30.384929589884152	31.0	31.0	34.0	26.0	34.0
3	30.4439357737627	33.0	31.0	34.0	26.0	34.0
4	33.89463723758875	37.0	35.0	37.0	30.0	37.0
5	33.89271757925916	37.0	35.0	37.0	30.0	37.0
6	33.928650301175374	37.0	35.0	37.0	30.0	37.0
7	33.83813145796798	37.0	35.0	37.0	29.0	37.0
8	33.805026542486495	37.0	35.0	37.0	29.0	37.0
9	35.356228486759925	39.0	37.0	39.0	29.0	39.0
10-11	35.24948019243469	39.0	35.0	39.0	29.0	39.0
12-13	35.14274694955209	39.0	35.0	39.0	28.0	39.0
14-15	36.27906853148269	40.0	37.0	41.0	27.5	41.0
16-17	36.21819836629244	40.0	36.5	41.0	27.0	41.0
18-19	36.17811877213261	40.0	36.5	41.0	27.0	41.0
20-21	36.10197219186034	40.0	36.5	41.0	27.0	41.0
22-23	36.002175532854366	40.0	36.0	41.0	27.0	41.0
24-25	35.8963396489897	40.0	36.0	41.0	27.0	41.0
26-27	35.754296920474836	40.0	36.0	41.0	27.0	41.0
28-29	35.615900104241234	40.0	36.0	41.0	26.0	41.0
30-31	35.47640013714128	40.0	36.0	41.0	25.0	41.0
32-33	35.30920511599867	39.5	35.0	41.0	25.0	41.0
34-35	35.138913760028856	39.0	35.0	41.0	24.0	41.0
36-37	35.11553167689321	39.0	35.0	41.0	24.5	41.0
38-39	35.29970126216337	39.5	35.0	41.0	25.0	41.0
40-41	35.36745419899286	40.0	35.0	41.0	24.5	41.0
42-43	35.217189253642914	40.0	35.0	41.0	24.0	41.0
44-45	35.10968092806396	40.0	35.0	41.0	23.5	41.0
46-47	34.95830483993124	40.0	35.0	41.0	23.0	41.0
48-49	34.80289110241294	39.0	35.0	41.0	22.0	41.0
50-51	33.117632751726056	37.0	32.5	39.5	18.5	40.0
52-53	33.241763197917415	37.5	33.0	39.5	18.0	40.0
54-55	34.15470285655069	39.0	34.0	40.5	20.0	41.0
56-57	34.17578220216192	39.0	34.0	41.0	19.5	41.0
58-59	33.985604826904414	38.0	34.0	41.0	18.5	41.0
60-61	33.73239587265468	38.0	34.0	40.0	17.5	41.0
62-63	33.438655114973045	37.0	34.0	40.0	14.0	41.0
64-65	33.1462711183062	37.0	33.0	40.0	13.0	41.0
66-67	32.80654846977444	36.0	33.0	39.5	9.5	41.0
68-69	32.44403953547785	36.0	33.0	39.0	7.5	41.0
70-71	32.03446114370185	35.0	32.0	39.0	4.5	40.5
72-73	31.670111804247064	35.0	32.0	37.5	2.0	40.0
74-75	31.27289277805143	35.0	32.0	37.0	2.0	39.0
76-77	30.897177068634313	35.0	32.0	36.5	2.0	39.0
78-79	30.562524358695324	35.0	31.0	36.0	2.0	38.5
80-81	30.254165187052138	35.0	31.0	36.0	2.0	37.0
82-83	29.95442343251213	35.0	31.0	35.0	2.0	37.0
84-85	29.70179788816272	35.0	31.0	35.0	2.0	36.0
86-87	29.476088585598617	35.0	31.0	35.0	2.0	36.0
88-89	29.26817581576821	35.0	31.0	35.0	2.0	36.0
90-91	29.057422585635383	34.0	30.0	35.0	2.0	35.0
92-93	28.877602201293463	34.0	30.0	35.0	2.0	35.0
94-95	28.66516640635219	34.0	30.0	35.0	2.0	35.0
96-97	28.441376762117677	34.0	30.0	35.0	2.0	35.0
98-99	28.189063035708152	34.0	30.0	35.0	2.0	35.0
100-101	27.13346367261061	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	134184.0
3	23085.0
4	15608.0
5	11867.0
6	10644.0
7	10862.0
8	11480.0
9	12222.0
10	13576.0
11	14239.0
12	14127.0
13	13959.0
14	14852.0
15	15322.0
16	15163.0
17	15776.0
18	15399.0
19	14995.0
20	15231.0
21	16204.0
22	17585.0
23	20388.0
24	23904.0
25	28565.0
26	33639.0
27	39515.0
28	47045.0
29	55629.0
30	66436.0
31	80836.0
32	101260.0
33	131442.0
34	181235.0
35	263468.0
36	441008.0
37	810225.0
38	861862.0
39	140939.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.20216362857576	17.704390454275643	12.999070034727767	25.094375882420835
2	15.953539968695704	19.443001095996905	39.34527581611246	25.25818311919493
3	18.745106381957736	23.887227580264668	29.159925237113036	28.20774080066456
4	13.246942701512973	15.776092075124021	36.39203588140199	34.58492934196102
5	14.527798304345554	37.03000824261657	34.24748536215797	14.194708090879912
6	33.82025432536692	34.99026610940909	17.211868446075684	13.977611119148309
7	29.281031506959536	31.326903022594188	21.202414110803762	18.189651359642507
8	26.7734924484871	34.56608676213114	19.914848808541443	18.745571980840317
9	27.151015101643374	15.271162215120826	18.96527700998917	38.612545673246636
10-11	25.78243094418718	24.89936486330047	28.021575224806313	21.296628967706035
12-13	26.356534925493403	22.377373804554303	27.717180017847582	23.548911252104713
14-15	23.706616431804832	23.83973686128678	24.650185762297017	27.803460944611373
16-17	24.027551238209544	26.81631735086872	25.449947331493423	23.70618407942832
18-19	24.047027234910644	26.414041457617287	26.475305919340585	23.063625388131477
20-21	24.730491734262724	25.599891286753174	26.142111928014778	23.527505050969328
22-23	24.556084694790865	25.358934557962197	25.89693497663646	24.18804577061048
24-25	24.304221600163732	25.55284382115416	25.88833434246096	24.254600236221144
26-27	24.157937095931644	25.72412393763337	25.94288223516091	24.175056731274083
28-29	24.29267775423297	25.879188093559286	25.74171140098741	24.086422751220336
30-31	24.59194047808718	25.643141344294495	25.63673377606388	24.128184401554442
32-33	24.30614732113301	25.679366124321145	25.62141990613778	24.39306664840806
34-35	24.61411096798944	25.717996432762636	25.67694256588	23.990950033367923
36-37	24.595779924832943	25.63558735053701	25.659456674513	24.109176050117057
38-39	24.498600368839274	25.6913538559289	25.65058039903641	24.159465376195413
40-41	24.568258868322218	25.565713682889186	25.69868723416612	24.16734021462248
42-43	24.383684332729082	25.54111247846879	25.650769609339964	24.424433579462164
44-45	24.325290025183833	25.579142745940125	25.72679144507794	24.368775783798096
46-47	24.533987964203355	25.526012153260723	25.562706329658663	24.377293552877255
48-49	24.27779068955616	25.58243504511476	25.68307571999332	24.45669854533576
50-51	24.350703219364103	25.573073025974274	25.72951158733216	24.34671216732946
52-53	24.52519836324873	25.52437325970364	25.70150153894292	24.2489268381047
54-55	24.104716769067384	25.319028913363496	26.005047865015797	24.571206452553326
56-57	24.173237564573764	25.458605712619768	25.837354808730574	24.530801914075894
58-59	24.34549105976493	25.4075215377207	25.82100732624939	24.425980076264977
60-61	24.392152747543676	25.36247876047557	25.91585790589604	24.32951058608471
62-63	24.31153912486778	25.384163170633645	25.960961898979996	24.343335805518578
64-65	24.14934789378831	25.23323928252632	25.95070762364175	24.666705200043623
66-67	24.131754439287985	25.4147426715324	25.808344923022176	24.64515796615744
68-69	24.149924999859852	25.360778837547215	25.859925525755116	24.629370636837816
70-71	24.141850317394262	25.148113318600522	25.899363823433706	24.810672540571506
72-73	24.09691400038416	25.273887421407743	25.795199550220627	24.833999027987474
74-75	24.02046139448811	25.346210046308165	25.886069334510804	24.747259224692918
76-77	24.078751974365087	25.05698530834798	25.948943192474605	24.915319524812336
78-79	23.965457582372515	25.014097202346115	26.03822691369522	24.982218301586148
80-81	24.071546252459953	25.175968427152007	25.869855460228198	24.88262986015984
82-83	23.9944362987448	25.263946035622535	25.904949253522506	24.836668412110154
84-85	24.01314625718427	25.208968109859732	25.899870195529083	24.878015437426914
86-87	23.923514936198128	25.236286780665807	25.91007181708043	24.93012646605564
88-89	23.91121424823319	25.039234981287112	25.941833155528617	25.107717614951085
90-91	23.893623567089225	24.999879822971224	25.99808357698113	25.108413032958417
92-93	23.889628979716584	24.935785233401	26.128990865794112	25.045594921088306
94-95	23.85084537369841	24.86574513353649	26.132156620930058	25.15125287183504
96-97	23.791428196378757	24.76716054648383	26.17192241627161	25.26948884086581
98-99	23.714281975471778	24.670470286710948	26.144978385649576	25.470269352167698
100-101	23.854820445161394	24.531923530951378	26.216795842169084	25.39646018171815
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	44.0
1	33.5
2	48.0
3	70.5
4	62.0
5	92.0
6	151.5
7	288.0
8	425.5
9	497.0
10	491.0
11	443.0
12	495.5
13	579.0
14	704.0
15	807.5
16	891.0
17	1040.0
18	1137.5
19	1212.0
20	1292.5
21	1378.5
22	1479.0
23	1625.5
24	1781.5
25	2001.5
26	2386.5
27	2978.0
28	3974.5
29	5529.0
30	8058.0
31	12134.0
32	18225.0
33	24843.0
34	31959.0
35	41054.5
36	52680.5
37	64954.5
38	77419.0
39	89601.5
40	100128.5
41	110270.5
42	120409.0
43	131967.5
44	143330.0
45	152989.0
46	159854.0
47	165633.0
48	172126.5
49	174666.5
50	172652.5
51	170778.0
52	169496.5
53	167941.0
54	164607.5
55	156951.0
56	144592.0
57	129026.5
58	116204.0
59	103275.0
60	85260.5
61	69268.0
62	56372.5
63	42748.0
64	30913.0
65	22365.5
66	16690.5
67	12814.0
68	9607.5
69	7092.0
70	5338.5
71	3885.5
72	2640.5
73	1879.5
74	1379.5
75	999.5
76	707.0
77	463.5
78	301.0
79	219.0
80	167.5
81	134.5
82	124.0
83	102.0
84	87.0
85	74.0
86	60.0
87	48.0
88	47.5
89	48.0
90	35.0
91	32.5
92	31.0
93	26.0
94	22.0
95	12.5
96	11.5
97	12.5
98	8.5
99	7.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0543450318118234
2	0.07645600063722213
3	0.0728329985164339
4	0.07376538876810736
5	0.06811776781511393
6	0.010975565248270217
7	0.014039133218054377
8	0.01875436391937435
9	0.006073856496615557
10-11	0.013493018927788506
12-13	0.024082308214651155
14-15	0.041331527870609806
16-17	0.028664340308589203
18-19	0.010482730400957112
20-21	0.021325097041845407
22-23	0.03922698987397547
24-25	0.03668289647298079
26-27	0.021111979270034338
28-29	0.011441760374106937
30-31	0.011162043298604905
32-33	0.008324912961370006
34-35	0.005008267637560196
36-37	5.594341510040644E-4
38-39	0.003343285045286194
40-41	0.0010389491375789768
42-43	0.23811915041665857
44-45	0.20616480450573593
46-47	0.21223866100235148
48-49	0.22082997117848532
50-51	0.21081343590336493
52-53	0.21851231541003993
54-55	0.21447639760636775
56-57	0.2177397634872248
58-59	0.2280759754200618
60-61	0.2320719336415194
62-63	0.21628789866676187
64-65	0.2163811376919292
66-67	0.21329093000066868
68-69	0.20778982751579536
70-71	0.19995774940173844
72-73	0.21195894392684947
74-75	0.21070687701745938
76-77	0.2038471487372905
78-79	0.2229478290358579
80-81	0.2345094681566085
82-83	0.23453610787808493
84-85	0.22762310015496326
86-87	0.24906807594345237
88-89	0.2410361999183226
90-91	0.2482022849954699
92-93	0.24328725638307708
94-95	0.2460444675558828
96-97	0.25383658608772514
98-99	0.24748301251560756
100-101	0.25395646483436884
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3753793.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.51196456080011
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.07247527907442	44.90101431424071
2	11.762908793760847	13.530159873573954
3	4.032571132329128	6.957632641542552
4	1.840678759381431	4.2344420630944954
5	1.0467448105421098	3.0100175224049623
6	0.6746133110205341	2.327900210139419
7	0.4561923254559056	1.836556179817032
8	0.33883304278988013	1.5589563159167723
9	0.2652528111169876	1.3729689245351517
>10	1.4115559011776608	14.418012044558287
>50	0.07136214770687271	2.7839157006530018
>100	0.025418757921978896	2.430721834054873
>500	0.0011607731082049803	0.4765210402271555
>1k	2.3215461393457507E-4	0.1611813352416827
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	2.663972147638402E-5
5	0.0	0.0	0.0	0.0	2.663972147638402E-5
6	0.0	0.0	0.0	0.0	2.663972147638402E-5
7	0.0	0.0	0.0	2.663972147638402E-5	2.663972147638402E-5
8	0.0	0.0	0.0	2.663972147638402E-5	5.327944295276804E-5
9	0.0	0.0	0.0	2.663972147638402E-5	5.327944295276804E-5
10-11	0.0	0.0	0.0	2.663972147638402E-5	5.327944295276804E-5
12-13	0.0	0.0	0.0	2.663972147638402E-5	1.5983832885830412E-4
14-15	0.0	0.0	0.0	2.663972147638402E-5	1.9979791107288015E-4
16-17	0.0	0.0	0.0	5.327944295276804E-5	2.1311777181107217E-4
18-19	0.0	0.0	0.0	1.0655888590553608E-4	2.3975749328745619E-4
20-21	0.0	1.331986073819201E-5	0.0	1.331986073819201E-4	2.530773540256482E-4
22-23	0.0	2.663972147638402E-5	0.0	1.7315818959649613E-4	2.663972147638402E-4
24-25	0.0	2.663972147638402E-5	0.0	2.663972147638402E-4	2.9303693624022423E-4
26-27	0.0	2.663972147638402E-5	0.0	4.7951498657491237E-4	3.596362399311843E-4
28-29	0.0	2.663972147638402E-5	0.0	0.0019047400855614575	3.729561006693763E-4
30-31	0.0	2.663972147638402E-5	0.0	0.008498071150966503	3.729561006693763E-4
32-33	0.0	2.663972147638402E-5	0.0	0.02638664412235837	3.995958221457603E-4
34-35	0.0	2.663972147638402E-5	0.0	0.051641100081970426	4.5287526509852835E-4
36-37	0.0	2.663972147638402E-5	0.0	0.09046849413380013	4.6619512583672036E-4
38-39	0.0	2.663972147638402E-5	0.0	0.1579069490512663	4.7951498657491237E-4
40-41	0.0	2.663972147638402E-5	0.0	0.2382257093025641	4.7951498657491237E-4
42-43	0.0	2.663972147638402E-5	0.0	0.324791484240074	5.194745687894884E-4
44-45	0.0	2.663972147638402E-5	0.0	0.4281402837077058	5.594341510040644E-4
46-47	0.0	2.663972147638402E-5	0.0	0.5507496018027632	5.860738724804485E-4
48-49	0.0	2.663972147638402E-5	0.0	0.679486055837389	6.393533154332165E-4
50-51	0.0	2.663972147638402E-5	0.0	0.8188117991588775	6.393533154332165E-4
52-53	0.0	2.663972147638402E-5	0.0	0.9638118031548356	7.192724798623686E-4
54-55	0.0	2.663972147638402E-5	0.0	1.1249421585047443	7.459122013387526E-4
56-57	1.331986073819201E-5	2.663972147638402E-5	0.0	1.3123525990911058	7.459122013387526E-4
58-59	2.663972147638402E-5	2.663972147638402E-5	0.0	1.5128831025045866	7.592320620769446E-4
60-61	2.663972147638402E-5	2.663972147638402E-5	0.0	1.7265070290237103	7.725519228151366E-4
62-63	2.663972147638402E-5	2.663972147638402E-5	0.0	1.9449394252693208	8.258313657679046E-4
64-65	2.663972147638402E-5	2.663972147638402E-5	0.0	2.184403881620537	8.391512265060967E-4
66-67	2.663972147638402E-5	2.663972147638402E-5	0.0	2.4564753570588467	8.524710872442887E-4
68-69	2.663972147638402E-5	2.663972147638402E-5	0.0	2.757557489184939	8.657909479824807E-4
70-71	2.663972147638402E-5	2.663972147638402E-5	0.0	3.0738642221347847	9.190703909352487E-4
72-73	2.663972147638402E-5	2.663972147638402E-5	0.0	3.4045430848211398	9.457101124116327E-4
74-75	2.663972147638402E-5	2.663972147638402E-5	0.0	3.7484885288027336	9.856696946262088E-4
76-77	2.663972147638402E-5	2.663972147638402E-5	0.0	4.133352584972053	0.0010389491375789768
78-79	2.663972147638402E-5	2.663972147638402E-5	0.0	4.5559118470304565	0.0010655888590553608
80-81	2.663972147638402E-5	2.663972147638402E-5	0.0	5.00668257413235	0.0010655888590553608
82-83	2.663972147638402E-5	2.663972147638402E-5	0.0	5.4689616609120435	0.001225427187913665
84-85	2.663972147638402E-5	2.663972147638402E-5	0.0	5.953871191085923	0.001225427187913665
86-87	2.663972147638402E-5	2.663972147638402E-5	0.0	6.470801666474417	0.001278706630866433
88-89	2.663972147638402E-5	3.995958221457603E-5	0.0	7.034991007762016	0.001305346352342817
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4990	0.0	45.271275	1
GGTATCA	2670	0.0	42.659554	1
TCAACGC	5920	0.0	37.4394	4
CAACGCA	6165	0.0	35.867382	5
ATCAACG	6360	0.0	34.923878	3
TATCAAC	6450	0.0	34.51061	2
AACGCAG	6765	0.0	32.809902	6
GTACATG	10975	0.0	29.145514	1
ACGCAGA	7695	0.0	28.294395	7
TACATGG	11525	0.0	27.756102	2
CGCAGAG	8030	0.0	26.407946	8
ACATGGG	11880	0.0	25.767778	3
CATGGGG	10440	0.0	23.821247	4
GCAGAGT	9555	0.0	20.25821	9
ATGGGGG	6030	0.0	19.830336	5
TATACCG	385	0.0	19.719961	5
AGAGTAC	7600	0.0	18.946596	10-11
CGTATAC	275	5.9667036E-7	17.258419	3
GAGTACA	8060	0.0	16.957966	1
CAGAGTA	8595	0.0	16.477242	10-11
>>END_MODULE