FastQCFastQC Report
Mon 15 Aug 2016
SRR2921687_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921687_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3753793
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26200.045.7322771
GTATCAA44200.040.2321241
TCAACGC57750.030.2414554
CAACGCA59350.029.5325155
GTACATG107950.028.7616711
TATCAAC61700.028.6899972
ATCAACG61700.028.3054123
AACGCAG64950.026.9822586
TACATGG113600.026.7367422
ACATGGG117400.025.386243
CATGGGG103350.023.0056294
TATACCG3550.021.4085065
ACGCAGA81100.021.3742797
CGCAGAG83500.020.5324218
ATGGGGG57900.019.5250955
AGAGTAC77600.017.59525710-11
TATTAAG14150.017.1049022
GCAGAGT99250.016.6042189
GTATAGG9950.016.2472341
GAGTACA81600.016.140361
Produced by FastQC (version 0.11.5)