##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921687_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3753793
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.440322361941643	33.0	31.0	34.0	29.0	34.0
2	31.573350475106114	34.0	31.0	34.0	29.0	34.0
3	31.64921747150149	34.0	31.0	34.0	28.0	34.0
4	35.12080234578731	37.0	35.0	37.0	33.0	37.0
5	34.86823700720844	37.0	35.0	37.0	32.0	37.0
6	34.8400372636424	37.0	35.0	37.0	32.0	37.0
7	34.72716529654139	37.0	35.0	37.0	32.0	37.0
8	34.672923893246114	37.0	35.0	37.0	32.0	37.0
9	36.27516088393793	39.0	37.0	39.0	32.0	39.0
10-11	36.16459058877248	39.0	37.0	39.0	32.0	39.0
12-13	36.089963271816	39.0	37.0	39.0	32.0	39.0
14-15	37.342791944041664	40.0	38.0	41.0	32.0	41.0
16-17	37.308586808063204	40.0	38.0	41.0	32.0	41.0
18-19	37.266311568059294	40.0	38.0	41.0	31.0	41.0
20-21	37.20653962538691	40.0	38.0	41.0	31.0	41.0
22-23	37.11909966798915	40.0	38.0	41.0	31.0	41.0
24-25	37.03749793875155	40.0	37.5	41.0	31.0	41.0
26-27	36.91333965405125	40.0	37.0	41.0	30.0	41.0
28-29	36.79740345298742	40.0	37.0	41.0	30.0	41.0
30-31	36.65160998488729	40.0	37.0	41.0	30.0	41.0
32-33	36.61055750809914	40.0	37.0	41.0	30.0	41.0
34-35	36.74978548364281	40.0	37.0	41.0	30.0	41.0
36-37	36.75456544886732	40.0	37.0	41.0	30.0	41.0
38-39	36.659765202822854	40.0	37.0	41.0	30.0	41.0
40-41	36.58559915264374	40.0	37.0	41.0	30.0	41.0
42-43	36.47981681461924	40.0	37.0	41.0	30.0	41.0
44-45	36.36010123626956	40.0	36.5	41.0	29.5	41.0
46-47	36.21651500229235	40.0	36.0	41.0	29.0	41.0
48-49	36.08028426181198	40.0	36.0	41.0	28.5	41.0
50-51	35.928729953942586	40.0	35.5	41.0	28.0	41.0
52-53	35.75430904154811	39.5	35.0	41.0	28.0	41.0
54-55	35.54318392090347	39.0	35.0	41.0	27.5	41.0
56-57	35.314395466132524	39.0	35.0	41.0	27.0	41.0
58-59	35.07084194573329	39.0	35.0	40.5	26.5	41.0
60-61	34.794016212401694	38.0	35.0	40.0	26.0	41.0
62-63	34.48591025131114	37.5	34.0	40.0	26.0	41.0
64-65	34.14533180172695	37.0	34.0	40.0	26.0	41.0
66-67	33.787062445904716	36.5	34.0	39.0	25.5	41.0
68-69	33.42311230267626	36.0	34.0	39.0	25.0	41.0
70-71	33.02346186910147	35.5	33.5	38.5	24.0	40.0
72-73	32.65241370528423	35.0	33.0	37.5	23.0	39.5
74-75	32.25040578955739	35.0	33.0	37.0	22.0	39.0
76-77	29.54640306484668	33.0	29.0	34.5	17.5	36.5
78-79	31.117760222793322	34.5	31.5	36.0	19.5	37.0
80-81	31.29745793121784	35.0	32.0	35.5	20.0	37.0
82-83	31.12638216864915	35.0	32.0	35.0	20.0	37.0
84-85	30.932029283447434	35.0	32.0	35.0	19.5	36.0
86-87	30.72943500081118	35.0	32.0	35.0	18.0	36.0
88-89	30.46854101970993	35.0	32.0	35.0	13.0	36.0
90-91	30.280533582965283	35.0	32.0	35.0	8.5	35.0
92-93	30.13048681693423	34.0	32.0	35.0	6.0	35.0
94-95	30.030077444334303	34.0	31.5	35.0	2.0	35.0
96-97	29.835819929335475	34.0	31.0	35.0	2.0	35.0
98-99	29.641651923800808	34.0	31.0	35.0	2.0	35.0
100-101	28.52492132624255	33.5	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	67655.0
3	23474.0
4	12809.0
5	9083.0
6	7930.0
7	7857.0
8	7940.0
9	8651.0
10	9601.0
11	10595.0
12	10905.0
13	11674.0
14	12535.0
15	13036.0
16	13920.0
17	14601.0
18	13970.0
19	13196.0
20	12938.0
21	12809.0
22	13696.0
23	15255.0
24	18538.0
25	22269.0
26	26486.0
27	32716.0
28	38990.0
29	47072.0
30	57739.0
31	72048.0
32	91466.0
33	121382.0
34	173616.0
35	260268.0
36	462779.0
37	918314.0
38	971998.0
39	113981.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.17938398306878	19.117483415821702	13.529177719894637	26.17395488121488
2	15.127435239434076	20.707050929074597	37.770823027080105	26.394690804411226
3	17.457371982735655	24.91759471412586	28.95436137901636	28.670671924122132
4	12.969096878197789	16.9484029732769	35.34342914703313	34.73907100149218
5	14.323038449598771	37.35282792875253	34.237822872575	14.0863107490737
6	33.864522091291335	35.44649985787429	16.972181455244787	13.716796595589601
7	29.14316266240573	31.542362618290355	21.17069321616829	18.143781503135628
8	26.79412530206114	34.96420820221041	19.661526354809656	18.5801401409188
9	26.884993392017087	15.21985895333067	18.871099178883867	39.024048475768375
10-11	25.684940634746837	25.151991360014158	27.9644378865085	21.198630118730506
12-13	26.289186822566368	22.525941177708557	27.695808572743058	23.48906342698202
14-15	23.553382526206562	24.02434467779867	24.62391695422452	27.79835584177025
16-17	23.96954227364162	27.01166526763729	25.34118157287842	23.677610885842668
18-19	23.975180993016025	26.613059629126823	26.42202328560133	22.98973609225582
20-21	24.633382688054542	25.679368891820452	26.136415099667587	23.550833320457418
22-23	24.466999112630877	25.45101714452555	25.91220400272471	24.16977974011886
24-25	24.277389893708207	25.733485837772978	25.827551258128327	24.161573010390487
26-27	24.09129095752567	25.86009688332056	25.905718652083863	24.14289350706991
28-29	24.17652752962721	26.00382592558107	25.77292768025588	24.046718864535833
30-31	24.50247205285365	25.822694287154597	25.57118595656562	24.10364770342613
32-33	24.276663522367397	25.82209482799848	25.56559087759786	24.33565077203626
34-35	24.447163760697148	25.847243075567995	25.703679695806024	24.00191346792883
36-37	24.4702996990648	25.82350907068141	25.659015055285124	24.04717617496867
38-39	24.44495474310917	25.83103277138617	25.59553763353493	24.128474851969727
40-41	24.496831763090206	25.680754675763556	25.654673499100213	24.16774006204603
42-43	24.362218034971754	25.647206608839983	25.58671571477764	24.40385964141062
44-45	24.28783286539548	25.674622431631278	25.69400421017521	24.343540492798038
46-47	24.449420625392367	25.680605819467644	25.523865443842205	24.346108111297788
48-49	24.17860754288345	25.71096763158564	25.6745878501121	24.435836975418805
50-51	24.2892258788621	25.71603561190072	25.655801532838762	24.33893697639842
52-53	24.494577843277273	25.59195634941725	25.624404174154243	24.289061633151235
54-55	24.065288258926923	25.4490419342021	25.853197324563514	24.632472482307463
56-57	24.13507806773059	25.55227402878404	25.70577062203352	24.60687728145185
58-59	24.242172336943973	25.532041026731374	25.747336647561696	24.478449988762957
60-61	24.25648979180795	25.5291719433669	25.746141065362615	24.468197199462534
62-63	24.215575643322964	25.535035538472812	25.806620081402087	24.442768736802137
64-65	24.049382702888682	25.362875506141524	25.756835400717776	24.83090639025202
66-67	24.034609786914174	25.629074993202792	25.655424172473175	24.680891047409865
68-69	24.046929809715127	25.5271261155345	25.64453326460373	24.781410810146642
70-71	24.023575320076638	25.32876716345735	25.733849682142644	24.91380783432337
72-73	24.020640480060248	25.491355296654604	25.547584042654968	24.94042018063018
74-75	23.91418841940432	25.543969994698628	25.619993159780414	24.921848426116636
76-77	23.898835359134317	25.21652942522542	25.79376321559113	25.09087200004913
78-79	23.831129061802347	25.232387713600325	25.804388243660014	25.132094980937314
80-81	23.938331958024445	25.42685113888505	25.55335467627903	25.081462226811475
82-83	23.81081238082143	25.464669849380673	25.637755237994742	25.08676253180316
84-85	23.8059141670035	25.392409169276377	25.654119674918185	25.147556988801938
86-87	23.699457791683724	25.467316284096952	25.619399592160818	25.21382633205851
88-89	23.64609025788846	25.253026850144032	25.690491630521883	25.410391261445625
90-91	23.578844873826664	25.20590225978782	25.745885495283684	25.469367371101832
92-93	23.600058426410445	25.160819227833148	25.80798767927739	25.43113466647902
94-95	23.476092977158014	25.090911028548817	25.83971311306164	25.593282881231527
96-97	23.38093383161275	25.049078355379518	25.8584182701505	25.711569542857234
98-99	23.236251549946125	24.96694314060344	25.840081124003817	25.956724185446618
100-101	23.20612395222422	24.9802399444827	25.84719156797739	25.966444535315684
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	31.0
1	32.5
2	48.0
3	65.0
4	59.5
5	62.0
6	110.5
7	267.0
8	374.0
9	370.5
10	320.0
11	267.5
12	276.0
13	302.0
14	321.5
15	350.5
16	411.0
17	467.5
18	534.0
19	606.0
20	646.0
21	740.5
22	916.5
23	1109.0
24	1343.0
25	1681.5
26	2175.5
27	2912.0
28	3978.0
29	5684.0
30	8540.5
31	12884.0
32	19029.5
33	25713.5
34	32926.5
35	41908.5
36	53251.5
37	65036.0
38	77093.0
39	89393.5
40	99565.0
41	109570.0
42	121050.5
43	132632.5
44	143348.5
45	153699.0
46	160569.0
47	166143.5
48	173328.5
49	176111.5
50	174024.5
51	172372.5
52	171375.0
53	169712.5
54	166567.0
55	158195.0
56	144974.5
57	129762.5
58	116796.5
59	102979.0
60	84374.5
61	68338.5
62	55455.5
63	41709.0
64	29512.5
65	21144.0
66	15659.0
67	11944.0
68	8939.0
69	6537.5
70	4834.0
71	3384.5
72	2277.0
73	1600.5
74	1138.5
75	786.5
76	489.5
77	273.5
78	145.0
79	77.5
80	56.0
81	36.5
82	20.5
83	15.5
84	13.5
85	9.5
86	6.0
87	4.0
88	3.0
89	1.5
90	2.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17038765856295218
2	1.0655888590553607E-4
3	0.01046941054021892
4	0.005967297610710021
5	0.025520853174375892
6	0.0019713393892524175
7	0.0
8	0.0
9	0.0
10-11	0.051547861056803077
12-13	3.1967665771660825E-4
14-15	0.005341264156014996
16-17	0.0
18-19	0.0015184641241538892
20-21	3.995958221457603E-4
22-23	0.0
24-25	5.993937332186405E-4
26-27	0.002730571451329362
28-29	0.00510150666272754
30-31	0.01696950258045662
32-33	0.011708157588870777
34-35	0.010615929008339032
36-37	0.004288995157697827
38-39	0.0
40-41	0.003409884348977154
42-43	0.008937626555326839
44-45	0.0071261254949327255
46-47	0.0035564028170972668
48-49	0.008910986833850455
50-51	0.002770531033543938
52-53	0.0019846592499906095
54-55	0.004328954739912403
56-57	0.007712199367413174
58-59	0.015490998038517308
60-61	0.007019566609027189
62-63	0.02179129216768213
64-65	0.04015938012564891
66-67	0.060019292486293195
68-69	0.050402353033318574
70-71	0.06878376085202353
72-73	0.0336726079461494
74-75	0.026360004400881987
76-77	0.23149917962977715
78-79	0.08716516867072852
80-81	0.06698557965236761
82-83	0.02719915562738808
84-85	0.05161446036049404
86-87	0.06818436711880489
88-89	0.29852471886435933
90-91	0.3026805154146752
92-93	0.055011024848733006
94-95	0.01060260914760084
96-97	0.016276869822070637
98-99	8.924306694588647E-4
100-101	2.1311777181107214E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3753793.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.442621839125884
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.14362012292094	43.541881778992085
2	12.32105070864762	13.908648116177833
3	4.139164197835583	7.008758386454479
4	1.9292322195796672	4.355636984383705
5	1.1006415685395698	3.1061547916750647
6	0.6926489578240479	2.345695391623643
7	0.48659250643050334	1.922518978114653
8	0.3436407710327177	1.5516788870324263
9	0.25290559788476724	1.2847189520167157
>10	1.486412985829445	14.95591768187422
>50	0.07701977463761822	2.946209432993804
>100	0.025697995932184938	2.4485517613132943
>500	0.0011359389601935635	0.4607758128730621
>1k	2.3665394516082805E-4	0.16285304447502055
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	2.663972147638402E-5	0.0	0.0	0.0
6	0.0	2.663972147638402E-5	0.0	0.0	0.0
7	0.0	2.663972147638402E-5	0.0	0.0	2.663972147638402E-5
8	0.0	2.663972147638402E-5	0.0	0.0	2.663972147638402E-5
9	0.0	2.663972147638402E-5	0.0	0.0	2.663972147638402E-5
10-11	0.0	2.663972147638402E-5	0.0	1.331986073819201E-5	2.663972147638402E-5
12-13	0.0	2.663972147638402E-5	0.0	5.327944295276804E-5	1.0655888590553608E-4
14-15	0.0	2.663972147638402E-5	0.0	5.327944295276804E-5	1.0655888590553608E-4
16-17	0.0	2.663972147638402E-5	0.0	5.327944295276804E-5	1.0655888590553608E-4
18-19	0.0	2.663972147638402E-5	0.0	5.327944295276804E-5	1.331986073819201E-4
20-21	0.0	2.663972147638402E-5	0.0	5.327944295276804E-5	1.4651846812011211E-4
22-23	0.0	2.663972147638402E-5	0.0	1.331986073819201E-4	2.3975749328745619E-4
24-25	0.0	2.663972147638402E-5	0.0	2.530773540256482E-4	2.9303693624022423E-4
26-27	0.0	2.663972147638402E-5	0.0	4.928348473131044E-4	2.9303693624022423E-4
28-29	0.0	2.663972147638402E-5	0.0	0.0019180599462996495	2.9303693624022423E-4
30-31	0.0	2.663972147638402E-5	0.0	0.008471431429490119	2.9303693624022423E-4
32-33	0.0	2.663972147638402E-5	0.0	0.026866159108933287	2.9303693624022423E-4
34-35	0.0	2.663972147638402E-5	0.0	0.05237369242257098	2.9303693624022423E-4
36-37	0.0	2.663972147638402E-5	0.0	0.09189371923278668	2.9303693624022423E-4
38-39	0.0	2.663972147638402E-5	0.0	0.15971845011166041	3.596362399311843E-4
40-41	0.0	2.663972147638402E-5	0.0	0.2410894793612754	3.729561006693763E-4
42-43	0.0	2.663972147638402E-5	0.0	0.32909379925851	3.729561006693763E-4
44-45	0.0	2.663972147638402E-5	0.0	0.4339610628502957	3.995958221457603E-4
46-47	0.0	2.663972147638402E-5	0.0	0.557316293146692	4.5287526509852835E-4
48-49	0.0	5.327944295276804E-5	0.0	0.686145986206485	4.7951498657491237E-4
50-51	0.0	5.327944295276804E-5	0.0	0.8263242006152177	5.061547080512964E-4
52-53	0.0	5.327944295276804E-5	0.0	0.9720301572303002	5.594341510040644E-4
54-55	0.0	5.327944295276804E-5	0.0	1.133733266591951	5.860738724804485E-4
56-57	0.0	5.327944295276804E-5	0.0	1.3216099023041494	5.860738724804485E-4
58-59	0.0	5.327944295276804E-5	0.0	1.5224467625146088	5.993937332186405E-4
60-61	0.0	5.327944295276804E-5	0.0	1.7369364799817144	6.393533154332165E-4
62-63	0.0	5.327944295276804E-5	0.0	1.9562080274538314	7.459122013387526E-4
64-65	0.0	5.327944295276804E-5	0.0	2.1970843890432956	7.725519228151366E-4
66-67	0.0	5.327944295276804E-5	0.0	2.4698884568222064	7.725519228151366E-4
68-69	0.0	5.327944295276804E-5	0.0	2.7722359757184263	7.725519228151366E-4
70-71	0.0	5.327944295276804E-5	0.0	3.089954613906521	8.524710872442887E-4
72-73	2.663972147638402E-5	5.327944295276804E-5	0.0	3.421619146287502	9.856696946262088E-4
74-75	2.663972147638402E-5	5.327944295276804E-5	0.0	3.768401720606331	0.0010123094161025928
76-77	2.663972147638402E-5	5.327944295276804E-5	0.0	4.156595741960199	0.0010389491375789768
78-79	2.663972147638402E-5	5.327944295276804E-5	0.0	4.582165292545433	0.0010389491375789768
80-81	2.663972147638402E-5	5.327944295276804E-5	0.0	5.037078496336905	0.0010789087197935528
82-83	2.663972147638402E-5	5.327944295276804E-5	0.0	5.504658887690398	0.001172147744960897
84-85	2.663972147638402E-5	5.327944295276804E-5	0.0	5.994097170515262	0.001172147744960897
86-87	2.663972147638402E-5	5.327944295276804E-5	0.0	6.5146772877460215	0.001185467605699089
88-89	2.663972147638402E-5	5.327944295276804E-5	0.0	7.085180243023523	0.001198787466437281
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2620	0.0	45.732277	1
GTATCAA	4420	0.0	40.232124	1
TCAACGC	5775	0.0	30.241455	4
CAACGCA	5935	0.0	29.532515	5
GTACATG	10795	0.0	28.761671	1
TATCAAC	6170	0.0	28.689997	2
ATCAACG	6170	0.0	28.305412	3
AACGCAG	6495	0.0	26.982258	6
TACATGG	11360	0.0	26.736742	2
ACATGGG	11740	0.0	25.38624	3
CATGGGG	10335	0.0	23.005629	4
TATACCG	355	0.0	21.408506	5
ACGCAGA	8110	0.0	21.374279	7
CGCAGAG	8350	0.0	20.532421	8
ATGGGGG	5790	0.0	19.525095	5
AGAGTAC	7760	0.0	17.595257	10-11
TATTAAG	1415	0.0	17.104902	2
GCAGAGT	9925	0.0	16.604218	9
GTATAGG	995	0.0	16.247234	1
GAGTACA	8160	0.0	16.14036	1
>>END_MODULE