FastQCFastQC Report
Mon 15 Aug 2016
SRR2921686_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921686_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3180307
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21650.043.401351
GTATCAA36200.041.9505161
TCAACGC43150.033.765564
CAACGCA44300.032.672715
ATCAACG46750.031.367973
TATCAAC47050.031.0675852
AACGCAG47550.030.319726
GTACATG81900.028.2769011
TACATGG83550.027.4357662
ACGCAGA55250.025.7564937
ACATGGG88250.024.7373243
CGCAGAG57450.024.6073578
CATGGGG78100.022.4228974
GTGTAGC21200.020.594341
GTATATA10250.020.3715821
GTATTAG7150.018.5843721
GCAGAGT72650.018.4144639
GTATAGC11300.018.0586761
TACCGTA1650.001478101717.2490467
TAATACT19050.017.1897894
Produced by FastQC (version 0.11.5)