##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921686_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3180307
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.01167088586102	31.0	31.0	34.0	26.0	34.0
2	30.075833873899594	31.0	31.0	34.0	26.0	34.0
3	30.13874320938199	31.0	31.0	34.0	26.0	34.0
4	33.56517153847097	37.0	35.0	37.0	28.0	37.0
5	33.55643307391394	37.0	35.0	37.0	28.0	37.0
6	33.59074328358866	37.0	35.0	37.0	28.0	37.0
7	33.49673632136772	37.0	35.0	37.0	28.0	37.0
8	33.46874405521228	37.0	35.0	37.0	28.0	37.0
9	34.992705735641245	39.0	35.0	39.0	28.0	39.0
10-11	34.87979572412349	39.0	35.0	39.0	27.0	39.0
12-13	34.76749351556312	39.0	35.0	39.0	27.0	39.0
14-15	35.87443193377243	40.0	36.0	41.0	27.0	41.0
16-17	35.81303896133298	40.0	36.0	41.0	27.0	41.0
18-19	35.77142867025102	40.0	36.0	41.0	27.0	41.0
20-21	35.69625510996266	40.0	36.0	41.0	26.5	41.0
22-23	35.59233888426495	40.0	36.0	41.0	26.0	41.0
24-25	35.486859759136465	40.0	36.0	41.0	25.0	41.0
26-27	35.34185677671998	40.0	36.0	41.0	24.5	41.0
28-29	35.20717308108934	40.0	35.5	41.0	24.0	41.0
30-31	35.0637807922317	39.0	35.0	41.0	24.0	41.0
32-33	34.89864799215925	39.0	35.0	41.0	23.0	41.0
34-35	34.731141993524524	39.0	35.0	41.0	22.5	41.0
36-37	34.703861765546534	39.0	35.0	41.0	22.0	41.0
38-39	34.895679882476756	39.5	35.0	41.0	22.5	41.0
40-41	34.96812336041772	40.0	35.0	41.0	22.5	41.0
42-43	34.81922499934755	40.0	35.0	41.0	21.0	41.0
44-45	34.71184873032698	40.0	35.0	41.0	19.5	41.0
46-47	34.564035641842125	39.5	35.0	41.0	18.0	41.0
48-49	34.41037280363185	39.0	35.0	41.0	15.5	41.0
50-51	32.747994926275986	37.0	32.0	39.5	13.5	40.0
52-53	32.869023179208796	37.5	32.5	39.5	12.5	40.0
54-55	33.78129281229768	38.5	34.0	40.5	13.0	41.0
56-57	33.80688955500208	39.0	34.0	41.0	10.5	41.0
58-59	33.62114537999005	38.0	34.0	41.0	8.0	41.0
60-61	33.375687472938935	38.0	33.5	40.0	7.5	41.0
62-63	33.088864062494594	37.0	33.0	40.0	4.0	41.0
64-65	32.804221101925066	37.0	33.0	40.0	2.0	41.0
66-67	32.46476598013965	36.0	33.0	39.5	2.0	41.0
68-69	32.10644679900399	36.0	32.0	39.0	2.0	41.0
70-71	31.702108318473655	35.0	32.0	39.0	2.0	40.5
72-73	31.336052934512296	35.0	32.0	37.5	2.0	40.0
74-75	30.935616278554242	35.0	31.5	37.0	2.0	39.0
76-77	30.563314956700722	35.0	31.0	36.5	2.0	39.0
78-79	30.226216840072357	35.0	31.0	36.0	2.0	38.5
80-81	29.91148307380388	35.0	31.0	36.0	2.0	37.0
82-83	29.60780138521218	35.0	31.0	35.0	2.0	37.0
84-85	29.350663945336095	35.0	30.0	35.0	2.0	36.5
86-87	29.12517455075878	35.0	30.0	35.0	2.0	36.0
88-89	28.918080707302785	34.0	30.0	35.0	2.0	36.0
90-91	28.709651929829416	34.0	30.0	35.0	2.0	35.5
92-93	28.532389168718616	34.0	30.0	35.0	2.0	35.0
94-95	28.3173820955021	34.0	29.5	35.0	2.0	35.0
96-97	28.096970040942587	34.0	29.0	35.0	2.0	35.0
98-99	27.845933427181713	34.0	29.0	35.0	2.0	35.0
100-101	26.80210574010622	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	136610.0
3	21662.0
4	14525.0
5	10618.0
6	9600.0
7	9516.0
8	10311.0
9	10916.0
10	11725.0
11	12458.0
12	12287.0
13	12479.0
14	13087.0
15	13433.0
16	13322.0
17	13764.0
18	13815.0
19	13137.0
20	13406.0
21	14047.0
22	15562.0
23	17659.0
24	20560.0
25	24314.0
26	28907.0
27	34439.0
28	40356.0
29	47361.0
30	57181.0
31	69324.0
32	86165.0
33	111313.0
34	152132.0
35	221106.0
36	365972.0
37	667268.0
38	721888.0
39	118075.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.18870391589444	17.684668341680084	13.002716391031946	25.123911351393524
2	15.82196800788993	19.17358383571119	39.70219537838596	25.302252778012924
3	19.172647347862767	23.638750597491214	28.711517868409388	28.477084186236635
4	13.108043216531387	15.716718619900574	36.73619148495622	34.43904667861182
5	14.569378050653114	36.9371316875225	34.33792488879826	14.155565373026121
6	33.807174281518385	35.09737513246981	17.326547105790308	13.768903480221512
7	29.447062127402102	31.065933848218275	21.019828175311623	18.467175849067996
8	26.597237509985096	34.89080868247089	19.724883166548207	18.787070640995804
9	26.740332881566992	15.681595919638	18.962174264412237	38.61589693438277
10-11	25.690801851079925	25.04079171702899	28.000186170927872	21.26822026096321
12-13	26.398202129019666	22.37612471118057	27.625188372463487	23.600484787336278
14-15	23.5793287881669	23.93091938408575	24.65113433343352	27.838617494313823
16-17	23.96962055893164	26.865681969511733	25.44341094682717	23.72128652472945
18-19	23.935529904848636	26.467946714230816	26.480509662621156	23.11601371829939
20-21	24.5721073579938	25.66918693361022	26.266584707277453	23.49212100111853
22-23	24.342602349758575	25.517780469008038	25.9571255563769	24.182491624856482
24-25	24.140072682961275	25.695205588790337	26.017178493807858	24.147543234440533
26-27	24.087611901549124	25.861132239487123	25.977733618292532	24.07352224067122
28-29	24.20007695098015	25.982858516644857	25.823814960408924	23.993249571966068
30-31	24.404819651443056	25.77396519650767	25.752895613265597	24.068319538783676
32-33	24.153425863688717	25.78744747506908	25.731316061524435	24.32781059971777
34-35	24.471599105384595	25.801651658143715	25.795645628530213	23.93110360794148
36-37	24.47357254381412	25.724861148297723	25.73680977611573	24.064756531772424
38-39	24.30360139652341	25.84492779189122	25.782588612038992	24.06888219954638
40-41	24.5045916129367	25.629171245701997	25.811247840188916	24.05498930117239
42-43	24.300300148853026	25.627984054050433	25.77756204113828	24.29415375595826
44-45	24.293147376865598	25.694469389465617	25.82181503545134	24.190568198217445
46-47	24.43279880180234	25.64431012093213	25.65678872060636	24.26610235665918
48-49	24.189299849977868	25.703844244222353	25.784488427055543	24.32236747874424
50-51	24.279349305901192	25.71974475528387	25.81911442668962	24.181791512125322
52-53	24.412728690542348	25.694755529532515	25.767425356254687	24.125090423670446
54-55	24.09878142164324	25.498224285223596	26.03033998556739	24.372654307565774
56-57	24.181203269577537	25.607310350825998	25.909287438068308	24.30219894152816
58-59	24.246379531583457	25.57448999225173	25.945058039534928	24.234072436629887
60-61	24.301485947300268	25.562034710712805	25.949827984497727	24.186651357489204
62-63	24.272891310746278	25.53825297275221	26.01926309400685	24.16959262249466
64-65	24.121617757052196	25.44707521571654	25.965727686733086	24.465579340498174
66-67	24.097226872418485	25.614840852996114	25.835833935342272	24.45209833924313
68-69	24.13908984433712	25.526021170353186	25.881848594761326	24.453040390548367
70-71	24.088419862646614	25.291136305132838	25.91104451145719	24.709399320763346
72-73	24.10941814594107	25.419439797394578	25.857053770034526	24.614088286629826
74-75	23.99149331464526	25.50962595288993	25.90020368652442	24.598677045940388
76-77	24.016511187005058	25.2533220406166	26.015879752732356	24.714287019645987
78-79	24.027152052090493	25.21685856404799	26.035019138263653	24.72097024559787
80-81	24.12201890815566	25.360599236298363	25.898373785145484	24.619008070400493
82-83	24.049989173351506	25.405652613666412	25.974343206963603	24.57001500601848
84-85	24.038616998980444	25.401305722898687	25.93213581661259	24.62794146150828
86-87	23.93551626893535	25.381528269280846	25.972655932302768	24.71029952948104
88-89	23.938665046080175	25.22374999763595	26.006725265830188	24.830859690453693
90-91	23.904866365696385	25.21680278523864	26.066250763839623	24.812080085225354
92-93	23.951583440690676	25.12349377936829	26.205472970694043	24.719449809246992
94-95	23.84114853410215	25.101676311547187	26.179230859919013	24.87794429443165
96-97	23.817974011104052	25.083639769482886	26.15760199401939	24.94078422539367
98-99	23.761508739273417	24.9443979046864	26.17720325913153	25.11689009690865
100-101	23.87377560661282	24.772365522822398	26.274691598354515	25.079167272210263
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	20.5
2	28.5
3	41.0
4	33.5
5	45.0
6	88.5
7	190.0
8	290.0
9	327.5
10	329.0
11	343.0
12	414.0
13	487.5
14	559.0
15	649.0
16	727.0
17	802.0
18	899.5
19	1019.5
20	1109.5
21	1147.0
22	1220.5
23	1375.5
24	1579.5
25	1810.0
26	2117.0
27	2663.5
28	3425.0
29	4590.0
30	6703.5
31	10035.0
32	15215.0
33	21456.0
34	28209.5
35	36568.5
36	47200.0
37	57909.0
38	68539.5
39	79387.0
40	88480.5
41	96840.0
42	105636.0
43	114418.0
44	122819.5
45	131182.0
46	136663.0
47	140677.0
48	145704.0
49	147724.5
50	146254.0
51	144162.0
52	142273.5
53	139952.5
54	136505.0
55	129896.0
56	119596.5
57	107853.5
58	97041.0
59	85034.5
60	70265.0
61	57228.5
62	46056.0
63	34770.5
64	24995.5
65	18113.5
66	13626.5
67	10376.0
68	7532.5
69	5344.0
70	3925.5
71	2747.0
72	1860.5
73	1322.0
74	987.5
75	702.5
76	478.5
77	329.0
78	240.0
79	174.0
80	135.5
81	113.0
82	93.0
83	80.5
84	71.5
85	64.0
86	59.5
87	51.0
88	40.5
89	41.5
90	33.5
91	26.5
92	29.0
93	21.5
94	19.0
95	19.0
96	15.0
97	13.5
98	10.0
99	7.0
100	5.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.057384397166688625
2	0.08131290469756536
3	0.07659637890304301
4	0.07700514447190161
5	0.07228861867737926
6	0.010596461285026886
7	0.01377225532000527
8	0.017828467503294494
9	0.00619437054347269
10-11	0.013630759546169598
12-13	0.024164333820602856
14-15	0.04167836627092919
16-17	0.028928024873070432
18-19	0.010093365200277834
20-21	0.02149163587037352
22-23	0.03952448615809732
24-25	0.036395857381064156
26-27	0.021082870301514917
28-29	0.011335383659502055
30-31	0.011555488196579766
32-33	0.008474024677491827
34-35	0.005329674147810258
36-37	8.018093850687999E-4
38-39	0.003898994656805145
40-41	0.0016350622754344158
42-43	0.2425709216122846
44-45	0.2094451887820893
46-47	0.21614265541031102
48-49	0.22351615740241432
50-51	0.2139258882868855
52-53	0.221692434095199
54-55	0.2181235962440104
56-57	0.22177104285844101
58-59	0.23081105063127555
60-61	0.23527602838342337
62-63	0.21963288449825757
64-65	0.2197114932614996
66-67	0.21710168232186391
68-69	0.21123746858400777
70-71	0.20281060916446117
72-73	0.2153251242725938
74-75	0.2139101665342371
76-77	0.2068982648530472
78-79	0.22681772545857995
80-81	0.23903352726639282
82-83	0.23834177014986288
84-85	0.23117265094218892
86-87	0.2535918702188185
88-89	0.24503923677808462
90-91	0.25190964268543886
92-93	0.2477748217389076
94-95	0.25107638979507324
96-97	0.2585127787977701
98-99	0.2512178855689089
100-101	0.25865427457160584
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3180307.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.694540227512874
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.59467102397917	51.334405156600084
2	11.096163244993019	14.13530032358515
3	3.509080687286978	6.705278429939668
4	1.5562079474832335	3.9648779885338414
5	0.8758737340225857	2.789418739296175
6	0.5282432906569379	2.0187728115997263
7	0.3593281605455179	1.6021069383721287
8	0.24936976863552845	1.2706794207904997
9	0.18397915963410003	1.0546621185904377
>10	0.9813048451773584	10.938447093020846
>50	0.04938666316255996	2.1478404609280437
>100	0.015498176336319308	1.6397649918512156
>500	8.436704153355572E-4	0.36244160348616705
>1k	4.9627671402671935E-5	0.03600392340600462
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	3.1443505296815685E-5	0.0
4	0.0	0.0	0.0	3.1443505296815685E-5	0.0
5	0.0	0.0	0.0	6.288701059363137E-5	0.0
6	0.0	0.0	0.0	6.288701059363137E-5	3.1443505296815685E-5
7	0.0	0.0	0.0	6.288701059363137E-5	3.1443505296815685E-5
8	0.0	0.0	0.0	6.288701059363137E-5	6.288701059363137E-5
9	0.0	0.0	0.0	1.5721752648407842E-4	6.288701059363137E-5
10-11	3.1443505296815685E-5	0.0	0.0	1.5721752648407842E-4	6.288701059363137E-5
12-13	3.1443505296815685E-5	1.5721752648407842E-5	0.0	1.5721752648407842E-4	9.433051589044706E-5
14-15	3.1443505296815685E-5	3.1443505296815685E-5	0.0	1.7293927913248627E-4	9.433051589044706E-5
16-17	3.1443505296815685E-5	3.1443505296815685E-5	0.0	2.201045370777098E-4	9.433051589044706E-5
18-19	3.1443505296815685E-5	3.1443505296815685E-5	0.0	2.515480423745255E-4	1.5721752648407842E-4
20-21	3.1443505296815685E-5	3.1443505296815685E-5	0.0	2.515480423745255E-4	2.0438278442930197E-4
22-23	3.1443505296815685E-5	3.1443505296815685E-5	0.0	2.829915476713412E-4	2.515480423745255E-4
24-25	3.1443505296815685E-5	3.1443505296815685E-5	0.0	3.7732206356178824E-4	2.829915476713412E-4
26-27	3.1443505296815685E-5	3.1443505296815685E-5	0.0	3.9304381621019604E-4	2.829915476713412E-4
28-29	3.1443505296815685E-5	3.1443505296815685E-5	0.0	0.0012577402118726273	2.829915476713412E-4
30-31	3.1443505296815685E-5	4.716525794522353E-5	0.0	0.005974266006394981	2.829915476713412E-4
32-33	3.1443505296815685E-5	6.288701059363137E-5	0.0	0.01866172039366011	2.829915476713412E-4
34-35	4.716525794522353E-5	6.288701059363137E-5	0.0	0.035877039543666694	3.7732206356178824E-4
36-37	6.288701059363137E-5	7.860876324203921E-5	0.0	0.0653553257594314	3.7732206356178824E-4
38-39	6.288701059363137E-5	9.433051589044706E-5	0.0	0.11898222404315055	4.2448732150701175E-4
40-41	6.288701059363137E-5	9.433051589044706E-5	0.0	0.17782874420614111	4.402090741554196E-4
42-43	6.288701059363137E-5	9.433051589044706E-5	0.0	0.24137606841100562	4.402090741554196E-4
44-45	6.288701059363137E-5	9.433051589044706E-5	0.0	0.3191673005153276	4.402090741554196E-4
46-47	6.288701059363137E-5	9.433051589044706E-5	0.0	0.40945732597513385	4.7165257945223525E-4
48-49	6.288701059363137E-5	9.433051589044706E-5	0.0	0.5076396712644408	4.7165257945223525E-4
50-51	6.288701059363137E-5	9.433051589044706E-5	0.0	0.6169215739235238	4.7165257945223525E-4
52-53	6.288701059363137E-5	9.433051589044706E-5	0.0	0.7284045219533837	5.03096084749051E-4
54-55	6.288701059363137E-5	1.5721752648407842E-4	0.0	0.8548388567518796	5.345395900458667E-4
56-57	6.288701059363137E-5	1.5721752648407842E-4	0.0	0.999070215548373	5.659830953426824E-4
58-59	6.288701059363137E-5	2.201045370777098E-4	0.0	1.1566336205907166	5.817048479910902E-4
60-61	6.288701059363137E-5	2.515480423745255E-4	0.0	1.3258940096034753	5.97426600639498E-4
62-63	6.288701059363137E-5	2.515480423745255E-4	0.0	1.5015374301914877	5.97426600639498E-4
64-65	6.288701059363137E-5	2.515480423745255E-4	0.0	1.6915033674421998	5.97426600639498E-4
66-67	6.288701059363137E-5	3.1443505296815683E-4	0.0	1.9078032403789948	6.131483532879059E-4
68-69	6.288701059363137E-5	3.1443505296815683E-4	0.0	2.1484089429102284	6.288701059363137E-4
70-71	6.288701059363137E-5	3.4587855826497254E-4	0.0	2.407314765524209	7.389223744751687E-4
72-73	6.288701059363137E-5	3.7732206356178824E-4	0.0	2.6798041824264134	8.175311377172078E-4
74-75	6.288701059363137E-5	4.087655688586039E-4	0.0	2.959179726988621	8.332528903656157E-4
76-77	6.288701059363137E-5	4.087655688586039E-4	0.0	3.270847751490658	8.489746430140235E-4
78-79	6.288701059363137E-5	4.5593082680382745E-4	0.0	3.621002626475997	8.489746430140235E-4
80-81	6.288701059363137E-5	5.03096084749051E-4	0.0	3.9936553295012085	8.804181483108392E-4
82-83	6.288701059363137E-5	5.03096084749051E-4	0.0	4.387406624580583	9.433051589044705E-4
84-85	6.288701059363137E-5	5.03096084749051E-4	0.0	4.796077862923296	9.433051589044705E-4
86-87	6.288701059363137E-5	5.03096084749051E-4	0.0	5.228378895496567	9.747486642012862E-4
88-89	6.288701059363137E-5	5.03096084749051E-4	0.0	5.696871402666472	0.0010376356747949177
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2165	0.0	43.40135	1
GTATCAA	3620	0.0	41.950516	1
TCAACGC	4315	0.0	33.76556	4
CAACGCA	4430	0.0	32.67271	5
ATCAACG	4675	0.0	31.36797	3
TATCAAC	4705	0.0	31.067585	2
AACGCAG	4755	0.0	30.31972	6
GTACATG	8190	0.0	28.276901	1
TACATGG	8355	0.0	27.435766	2
ACGCAGA	5525	0.0	25.756493	7
ACATGGG	8825	0.0	24.737324	3
CGCAGAG	5745	0.0	24.607357	8
CATGGGG	7810	0.0	22.422897	4
GTGTAGC	2120	0.0	20.59434	1
GTATATA	1025	0.0	20.371582	1
GTATTAG	715	0.0	18.584372	1
GCAGAGT	7265	0.0	18.414463	9
GTATAGC	1130	0.0	18.058676	1
TACCGTA	165	0.0014781017	17.249046	7
TAATACT	1905	0.0	17.189789	4
>>END_MODULE