FastQCFastQC Report
Mon 15 Aug 2016
SRR2921686_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921686_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3180307
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA38200.042.567871
GGTATCA21350.040.3088041
TCAACGC45450.035.0891274
CAACGCA46150.034.277475
ATCAACG47450.033.812323
TATCAAC51100.031.2113762
AACGCAG51100.031.044156
ACGCAGA60750.025.4080167
GTACATG81350.024.6646041
CGCAGAG64750.023.7650598
TACATGG84300.022.6356532
ACATGGG86350.021.7134723
CATGGGG74600.019.4056514
GCAGAGT77350.018.9117159
CTAATAC8400.018.082783
AGAGTAC62950.017.5415410-11
GTATTAT9850.016.894721
CCTAATA8350.016.485652
TAATACT18000.016.0851294
GAGTACA63450.016.0361961
Produced by FastQC (version 0.11.5)