##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921686_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3180307
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.315756623495783	33.0	31.0	34.0	28.0	34.0
2	31.45241795839207	34.0	31.0	34.0	28.0	34.0
3	31.529862368632966	34.0	31.0	34.0	28.0	34.0
4	34.995228133636154	37.0	35.0	37.0	32.0	37.0
5	34.734624361736145	37.0	35.0	37.0	32.0	37.0
6	34.70746000307518	37.0	35.0	37.0	32.0	37.0
7	34.589716338705664	37.0	35.0	37.0	32.0	37.0
8	34.535266878323384	37.0	35.0	37.0	32.0	37.0
9	36.12731569625196	39.0	37.0	39.0	32.0	39.0
10-11	36.013155333746084	39.0	37.0	39.0	31.0	39.0
12-13	35.93686395684442	39.0	37.0	39.0	31.0	39.0
14-15	37.1808749909993	40.0	38.0	41.0	31.0	41.0
16-17	37.14383988715555	40.0	38.0	41.0	31.0	41.0
18-19	37.10145718636597	40.0	38.0	41.0	31.0	41.0
20-21	37.04498968181373	40.0	38.0	41.0	31.0	41.0
22-23	36.95717127308779	40.0	37.5	41.0	30.5	41.0
24-25	36.87854631644052	40.0	37.0	41.0	30.0	41.0
26-27	36.75332805920938	40.0	37.0	41.0	30.0	41.0
28-29	36.63502312827032	40.0	37.0	41.0	30.0	41.0
30-31	36.49335268576273	40.0	37.0	41.0	30.0	41.0
32-33	36.45658453727894	40.0	37.0	41.0	30.0	41.0
34-35	36.593319607195156	40.0	37.0	41.0	30.0	41.0
36-37	36.60059060335999	40.0	37.0	41.0	30.0	41.0
38-39	36.50198534292444	40.0	37.0	41.0	30.0	41.0
40-41	36.43291889745235	40.0	37.0	41.0	30.0	41.0
42-43	36.32544672574063	40.0	37.0	41.0	29.0	41.0
44-45	36.20969264916877	40.0	36.5	41.0	29.0	41.0
46-47	36.068896964978535	40.0	36.0	41.0	28.0	41.0
48-49	35.93832985306136	40.0	36.0	41.0	28.0	41.0
50-51	35.78640835617442	40.0	35.5	41.0	28.0	41.0
52-53	35.622746011627186	39.5	35.0	41.0	27.0	41.0
54-55	35.41774221796827	39.0	35.0	41.0	27.0	41.0
56-57	35.19370535611814	39.0	35.0	41.0	26.0	41.0
58-59	34.956776499878785	39.0	35.0	40.5	26.0	41.0
60-61	34.683200238215996	38.0	34.5	40.0	26.0	41.0
62-63	34.382734119693474	37.5	34.0	40.0	26.0	41.0
64-65	34.04597449239964	37.0	34.0	40.0	25.0	41.0
66-67	33.692842074680215	36.5	34.0	39.5	24.0	41.0
68-69	33.327807032465735	36.0	34.0	39.0	23.0	41.0
70-71	32.9279956306105	35.5	33.0	39.0	22.0	40.0
72-73	32.561580532948554	35.0	33.0	37.5	22.0	40.0
74-75	32.15124892030864	35.0	33.0	37.0	20.5	39.0
76-77	29.448230469574163	33.0	29.0	34.5	14.5	36.5
78-79	31.004697974126397	34.5	31.5	36.0	18.0	37.0
80-81	31.177642127002205	35.0	32.0	36.0	18.5	37.0
82-83	30.996113582745313	35.0	32.0	35.0	18.0	37.0
84-85	30.79590555251427	35.0	32.0	35.0	17.5	36.0
86-87	30.583455307930965	35.0	32.0	35.0	13.5	36.0
88-89	30.31645954305669	35.0	32.0	35.0	7.0	36.0
90-91	30.12159989585911	34.5	31.5	35.0	4.5	35.0
92-93	29.96483326924099	34.0	31.0	35.0	2.0	35.0
94-95	29.859114072949563	34.0	31.0	35.0	2.0	35.0
96-97	29.661557044650092	34.0	31.0	35.0	2.0	35.0
98-99	29.461160196169743	34.0	31.0	35.0	2.0	35.0
100-101	28.356263719194406	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65433.0
3	20965.0
4	11172.0
5	7895.0
6	6853.0
7	6831.0
8	6964.0
9	7610.0
10	8323.0
11	8927.0
12	9563.0
13	10117.0
14	10693.0
15	11328.0
16	12132.0
17	12774.0
18	12220.0
19	11451.0
20	10965.0
21	11149.0
22	11624.0
23	13279.0
24	15696.0
25	19211.0
26	22878.0
27	27900.0
28	33024.0
29	40975.0
30	49320.0
31	61677.0
32	78275.0
33	103125.0
34	145925.0
35	218407.0
36	386035.0
37	767486.0
38	826284.0
39	95821.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.74305751755405	19.34587731490887	13.631319826804486	26.279745340732603
2	14.931465671206276	20.618877943743744	37.995958876887244	26.453697508162744
3	17.62083201255868	24.784367814356948	28.61442562655034	28.980374546534037
4	12.764557793138492	17.076316036071617	35.66122389169207	34.49790227909782
5	14.312758317342727	37.225941044909675	34.396162128857284	14.065138508890316
6	33.82297035418947	35.72511820622738	16.96066374718693	13.491247692396218
7	29.231894908258855	31.24742359778474	21.115760208055388	18.404921285901015
8	26.53149522986303	35.330897300166306	19.50126198508509	18.636345484885577
9	26.506694638817773	15.605196481093328	18.89302475925273	38.99508412083617
10-11	25.567337493076835	25.357829752112654	28.00888366932855	21.065949085481957
12-13	26.28596107312636	22.49972604755563	27.691820558240405	23.52249232107761
14-15	23.380112551241663	24.099653742911304	24.70879138934104	27.811442316505993
16-17	23.878842514260416	27.06994953631835	25.40776723756543	23.643440711855803
18-19	23.83055460131766	26.659460751212755	26.46334455621911	23.046640091250474
20-21	24.517132589912553	25.790597958021866	26.23855905349765	23.453710398567935
22-23	24.176034605469628	25.653282160068535	25.970232743290623	24.200450491171214
24-25	24.039304622616665	25.905095058229797	26.00373393914858	24.051866380004952
26-27	23.982981723322983	26.034076802040605	25.95175566028599	24.031185814350415
28-29	24.08800106032951	26.15671273953518	25.79809684688757	23.95718935324774
30-31	24.32527161081452	25.898085071622106	25.752980670515047	24.023662647048326
32-33	24.028040032660854	25.964341931415035	25.675579847315376	24.33203818860873
34-35	24.287856260550395	25.97024317164658	25.83838683059243	23.903513737210595
36-37	24.343133426032665	25.86056611996702	25.81454662543622	23.9817538285641
38-39	24.275175993257253	25.964793016019055	25.77449689204484	23.985534098678855
40-41	24.366915115284364	25.765285627341537	25.8049843697011	24.062814887673
42-43	24.215853001997477	25.74020067823558	25.790609229639095	24.25333709012785
44-45	24.173750838811685	25.81733433036885	25.78797985216789	24.220934978651574
46-47	24.316982418340547	25.80027476354393	25.61971945692536	24.263023361190168
48-49	24.132488120782565	25.830365985713648	25.758354052487263	24.27879184101652
50-51	24.16813655765497	25.856366429476914	25.773274811259395	24.202222201608723
52-53	24.364194298128456	25.82587804397566	25.700604341846905	24.10932331604898
54-55	24.046274819196242	25.611534838732908	25.953685874784544	24.388504467286307
56-57	24.069706871836832	25.74723837714357	25.837236693212816	24.34581805780678
58-59	24.178800883635894	25.71495870909532	25.8416484095859	24.264591997682885
60-61	24.194019311744917	25.76087969961814	25.817922191085742	24.2271787975512
62-63	24.12771466549547	25.763177430978658	25.852290998649064	24.25681690487681
64-65	23.99328600444601	25.59619582193442	25.83845517706496	24.57206299655461
66-67	23.961356867232546	25.822344849892186	25.719477511318317	24.496820771556955
68-69	24.00307799124578	25.756674158731418	25.70154149664808	24.538706353374725
70-71	23.952136223942837	25.52627215147853	25.778784611119796	24.742807013458837
72-73	23.988086400958224	25.693408202756395	25.643285908762643	24.675219487522742
74-75	23.922146417837897	25.731387025231207	25.617987105024742	24.728479451906153
76-77	23.8837023609711	25.466586592971975	25.8497206271655	24.799990418891422
78-79	23.8939097472839	25.508960835730992	25.781999022840512	24.815130394144596
80-81	23.988234648161956	25.671181511356227	25.608896760705264	24.731687079776556
82-83	23.941528770439106	25.661499306167997	25.649846325332355	24.747125598060542
84-85	23.982176187580396	25.62489205387538	25.62913911451617	24.76379264402805
86-87	23.828451260794125	25.746344089557045	25.613201662400332	24.8120029872485
88-89	23.800163144098686	25.558369587139463	25.632404145746058	25.00906312301579
90-91	23.748096247182417	25.575635874729674	25.672065350642175	25.004202527445734
92-93	23.750060167752412	25.554321963699262	25.761991157778564	24.93362671076976
94-95	23.63421411661006	25.484454911278853	25.75294619498197	25.12838477712912
96-97	23.594458814557402	25.521105721226796	25.747832034781787	25.13660342943401
98-99	23.543088874365367	25.3615442532367	25.71187487648499	25.383491995912937
100-101	23.480599314530075	25.384224758670566	25.75200452787473	25.38317139892463
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	16.0
1	14.0
2	22.5
3	42.0
4	42.5
5	35.0
6	62.5
7	173.5
8	256.0
9	242.5
10	208.0
11	187.0
12	215.0
13	235.5
14	254.5
15	276.5
16	300.0
17	340.5
18	387.5
19	462.5
20	525.5
21	610.0
22	746.5
23	969.5
24	1222.5
25	1533.0
26	1959.0
27	2647.0
28	3645.0
29	5159.5
30	7596.0
31	11152.5
32	16469.5
33	22579.0
34	29446.0
35	37729.0
36	47934.0
37	58753.0
38	69189.0
39	79657.5
40	88777.5
41	96916.5
42	105708.5
43	114480.5
44	122688.0
45	131496.5
46	137458.5
47	141852.5
48	146885.0
49	148731.0
50	147360.0
51	144987.0
52	142911.0
53	141030.0
54	137516.5
55	130541.0
56	120137.5
57	108129.5
58	96597.5
59	84163.0
60	69591.5
61	56153.5
62	45047.5
63	33601.0
64	23501.0
65	16782.0
66	12380.5
67	9536.5
68	6938.5
69	4869.0
70	3463.0
71	2303.5
72	1498.5
73	1020.0
74	730.0
75	472.0
76	302.0
77	172.5
78	83.0
79	53.0
80	43.5
81	31.0
82	19.5
83	13.5
84	7.5
85	5.0
86	4.5
87	3.0
88	4.5
89	5.5
90	4.0
91	2.0
92	2.0
93	2.0
94	2.0
95	1.0
96	0.5
97	0.5
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.16973204159221109
2	9.433051589044706E-5
3	0.011162444380369568
4	0.005942822501098164
5	0.02628677042813791
6	0.001886610317808941
7	0.0
8	0.0
9	3.1443505296815685E-5
10-11	0.05236915807184653
12-13	3.301568056165647E-4
14-15	0.004889465073654839
16-17	0.0
18-19	0.0015564535121923764
20-21	2.6726979502293333E-4
22-23	1.5721752648407842E-5
24-25	6.131483532879059E-4
26-27	0.002484036918448439
28-29	0.005141013116029364
30-31	0.016586449044070275
32-33	0.011115279122424344
34-35	0.010407800253245992
36-37	0.0041819862044764865
38-39	1.5721752648407842E-5
40-41	0.0033172898088140545
42-43	0.009150060041373365
44-45	0.006697466628221741
46-47	0.003710333625024251
48-49	0.008757016225163167
50-51	0.002594089186987294
52-53	0.0022167671234255056
54-55	0.004166264451828078
56-57	0.007782267560961882
58-59	0.015423039348088093
60-61	0.006964736423244674
62-63	0.0206426612273595
64-65	0.04012191275873682
66-67	0.06104756553376765
68-69	0.05063976528052166
70-71	0.07062211289664803
72-73	0.034053316236451386
74-75	0.026774144760238556
76-77	0.2325718869278972
78-79	0.08582504770765842
80-81	0.0679651366990671
82-83	0.027135745071151934
84-85	0.05150446167618409
86-87	0.06702183154016264
88-89	0.29841458701942924
90-91	0.3017318768282433
92-93	0.05326529797280577
94-95	0.009920425921145348
96-97	0.01515576955306516
98-99	8.018093850687999E-4
100-101	2.201045370777098E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3180307.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.25927671848341
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44165053792294	49.459797038136
2	11.636173849201887	14.489195352436813
3	3.837477538021218	7.1675572782188235
4	1.6756302283707127	4.17294104263951
5	0.9133786348594303	2.8433146588231946
6	0.5442720566816199	2.0331590752247433
7	0.36967612824541146	1.6111037855254042
8	0.26970143121543916	1.3433132829930436
9	0.19570182661426597	1.096582875974122
>10	1.045644749485864	11.431213180663036
>50	0.052976517814138376	2.239850655185624
>100	0.01680503661716368	1.7137013704348658
>500	8.101910669642908E-4	0.32645373492586344
>1k	1.0127388293941833E-4	0.07181666881897537
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.1443505296815685E-5
2	0.0	0.0	0.0	0.0	3.1443505296815685E-5
3	0.0	0.0	0.0	0.0	3.1443505296815685E-5
4	0.0	0.0	0.0	3.1443505296815685E-5	3.1443505296815685E-5
5	0.0	0.0	0.0	6.288701059363137E-5	3.1443505296815685E-5
6	0.0	0.0	0.0	6.288701059363137E-5	3.1443505296815685E-5
7	0.0	0.0	0.0	6.288701059363137E-5	3.1443505296815685E-5
8	0.0	0.0	0.0	6.288701059363137E-5	3.1443505296815685E-5
9	0.0	0.0	0.0	6.288701059363137E-5	9.433051589044706E-5
10-11	3.1443505296815685E-5	0.0	0.0	6.288701059363137E-5	9.433051589044706E-5
12-13	3.1443505296815685E-5	0.0	0.0	6.288701059363137E-5	9.433051589044706E-5
14-15	3.1443505296815685E-5	0.0	0.0	7.860876324203921E-5	1.100522685388549E-4
16-17	3.1443505296815685E-5	0.0	0.0	9.433051589044706E-5	1.2577402118726274E-4
18-19	3.1443505296815685E-5	0.0	0.0	9.433051589044706E-5	1.2577402118726274E-4
20-21	3.1443505296815685E-5	3.1443505296815685E-5	0.0	9.433051589044706E-5	1.5721752648407842E-4
22-23	3.1443505296815685E-5	3.1443505296815685E-5	0.0	1.5721752648407842E-4	1.5721752648407842E-4
24-25	7.860876324203921E-5	3.1443505296815685E-5	0.0	2.515480423745255E-4	1.5721752648407842E-4
26-27	9.433051589044706E-5	3.1443505296815685E-5	0.0	2.8299154767134113E-4	1.5721752648407842E-4
28-29	9.433051589044706E-5	3.1443505296815685E-5	0.0	0.0010690791800917333	1.5721752648407842E-4
30-31	9.433051589044706E-5	3.1443505296815685E-5	0.0	0.005801326727262494	1.5721752648407842E-4
32-33	9.433051589044706E-5	3.1443505296815685E-5	0.0	0.018630276888363294	1.5721752648407842E-4
34-35	9.433051589044706E-5	3.1443505296815685E-5	0.0	0.036191474596634854	1.8866103178089412E-4
36-37	9.433051589044706E-5	3.1443505296815685E-5	0.0	0.06610996988655497	1.8866103178089412E-4
38-39	9.433051589044706E-5	3.1443505296815685E-5	0.0	0.12069589508182701	1.8866103178089412E-4
40-41	1.100522685388549E-4	3.1443505296815685E-5	0.0	0.18009267658751182	1.8866103178089412E-4
42-43	1.2577402118726274E-4	3.1443505296815685E-5	0.0	0.243860105329454	2.0438278442930197E-4
44-45	1.2577402118726274E-4	3.1443505296815685E-5	0.0	0.32224876403441555	2.3582628972611762E-4
46-47	1.2577402118726274E-4	3.1443505296815685E-5	0.0	0.4124444589783314	2.6726979502293333E-4
48-49	1.2577402118726274E-4	3.1443505296815685E-5	0.0	0.5110512915891453	3.1443505296815683E-4
50-51	1.2577402118726274E-4	3.1443505296815685E-5	0.0	0.6205375770326576	3.1443505296815683E-4
52-53	1.2577402118726274E-4	3.1443505296815685E-5	0.0	0.7320048033098692	3.1443505296815683E-4
54-55	1.2577402118726274E-4	7.860876324203921E-5	0.0	0.859366721514621	3.301568056165647E-4
56-57	1.2577402118726274E-4	9.433051589044706E-5	0.0	1.0037081325796535	3.4587855826497254E-4
58-59	1.2577402118726274E-4	9.433051589044706E-5	0.0	1.1615388074170199	3.4587855826497254E-4
60-61	1.2577402118726274E-4	1.4149577383567056E-4	0.0	1.331192240245989	3.4587855826497254E-4
62-63	1.2577402118726274E-4	1.7293927913248627E-4	0.0	1.5084235578514904	4.402090741554196E-4
64-65	1.2577402118726274E-4	2.829915476713412E-4	0.0	1.699490017787591	4.402090741554196E-4
66-67	1.2577402118726274E-4	3.1443505296815683E-4	0.0	1.9168589699044778	4.402090741554196E-4
68-69	1.2577402118726274E-4	3.1443505296815683E-4	0.0	2.15886390842142	4.402090741554196E-4
70-71	1.2577402118726274E-4	3.4587855826497254E-4	0.0	2.4174395742297836	4.5593082680382745E-4
72-73	1.2577402118726274E-4	3.7732206356178824E-4	0.0	2.69109240082797	4.7165257945223525E-4
74-75	1.2577402118726274E-4	3.7732206356178824E-4	0.0	2.9729677040612748	4.873743321006431E-4
76-77	1.2577402118726274E-4	3.7732206356178824E-4	0.0	3.2887076624992493	5.03096084749051E-4
78-79	1.2577402118726274E-4	3.7732206356178824E-4	0.0	3.6429816366784715	5.03096084749051E-4
80-81	1.2577402118726274E-4	3.7732206356178824E-4	0.0	4.020162204466424	5.502613426942745E-4
82-83	1.2577402118726274E-4	3.7732206356178824E-4	0.0	4.416963519559589	6.288701059363137E-4
84-85	1.2577402118726274E-4	3.7732206356178824E-4	0.0	4.829282204516733	6.603136112331294E-4
86-87	1.2577402118726274E-4	3.7732206356178824E-4	0.0	5.2655765622627	6.603136112331294E-4
88-89	1.2577402118726274E-4	3.7732206356178824E-4	0.0	5.7379523423367615	6.603136112331294E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3820	0.0	42.56787	1
GGTATCA	2135	0.0	40.308804	1
TCAACGC	4545	0.0	35.089127	4
CAACGCA	4615	0.0	34.27747	5
ATCAACG	4745	0.0	33.81232	3
TATCAAC	5110	0.0	31.211376	2
AACGCAG	5110	0.0	31.04415	6
ACGCAGA	6075	0.0	25.408016	7
GTACATG	8135	0.0	24.664604	1
CGCAGAG	6475	0.0	23.765059	8
TACATGG	8430	0.0	22.635653	2
ACATGGG	8635	0.0	21.713472	3
CATGGGG	7460	0.0	19.405651	4
GCAGAGT	7735	0.0	18.911715	9
CTAATAC	840	0.0	18.08278	3
AGAGTAC	6295	0.0	17.54154	10-11
GTATTAT	985	0.0	16.89472	1
CCTAATA	835	0.0	16.48565	2
TAATACT	1800	0.0	16.085129	4
GAGTACA	6345	0.0	16.036196	1
>>END_MODULE