FastQCFastQC Report
Mon 15 Aug 2016
SRR2921685_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921685_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3060839
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA24100.049.432591
GTATCAA42650.042.0658261
TCAACGC51700.033.602814
CAACGCA52750.032.841815
TATCAAC54050.032.4063382
ATCAACG53650.032.381463
GTACATG84250.030.59051
AACGCAG57550.030.0863826
TACATGG87350.028.7469672
ACATGGG87950.027.8483163
ACGCAGA67500.025.0920737
CATGGGG77350.024.7916894
CGCAGAG67450.024.5499748
ATGGGGG44500.019.8385375
AATACCG2902.4665496E-919.639935
GCAGAGT80200.019.5834679
GTATATA8300.019.4427411
TAAGGCG6700.019.1269455
AGAGTAC66450.018.60183510-11
ATACCGT3059.430005E-817.1086316
Produced by FastQC (version 0.11.5)