##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921685_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3060839
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.326901872329778	31.0	31.0	34.0	26.0	34.0
2	30.393883507103773	31.0	31.0	34.0	26.0	34.0
3	30.451526199189175	33.0	31.0	34.0	26.0	34.0
4	33.907461646953664	37.0	35.0	37.0	30.0	37.0
5	33.907361020948834	37.0	35.0	37.0	30.0	37.0
6	33.944350225542735	37.0	35.0	37.0	30.0	37.0
7	33.85695948071754	37.0	35.0	37.0	29.0	37.0
8	33.8280327714068	37.0	35.0	37.0	29.0	37.0
9	35.37916270669578	39.0	37.0	39.0	30.0	39.0
10-11	35.27367022571262	39.0	35.0	39.0	29.0	39.0
12-13	35.16653342433235	39.0	35.0	39.0	28.0	39.0
14-15	36.29352834304581	40.0	37.0	41.0	28.0	41.0
16-17	36.232283534024496	40.0	36.0	41.0	27.0	41.0
18-19	36.207332858735796	40.0	36.5	41.0	27.0	41.0
20-21	36.129247895756684	40.0	36.5	41.0	27.0	41.0
22-23	36.02763147620636	40.0	36.0	41.0	27.0	41.0
24-25	35.91890344444775	40.0	36.0	41.0	27.0	41.0
26-27	35.77386134978024	40.0	36.0	41.0	27.0	41.0
28-29	35.63504924630142	40.0	36.0	41.0	26.0	41.0
30-31	35.48770239140314	40.0	36.0	41.0	25.0	41.0
32-33	35.3109315779105	39.0	35.0	41.0	25.0	41.0
34-35	35.13514497168914	39.0	35.0	41.0	24.5	41.0
36-37	35.10373364949937	39.0	35.0	41.0	24.5	41.0
38-39	35.284054306678655	39.5	35.0	41.0	24.5	41.0
40-41	35.34687286721059	40.0	35.0	41.0	24.5	41.0
42-43	35.19152379461971	40.0	35.0	41.0	24.0	41.0
44-45	35.0746363987129	40.0	35.0	41.0	23.5	41.0
46-47	34.91636753844289	40.0	35.0	41.0	22.5	41.0
48-49	34.752033674427175	39.0	35.0	41.0	22.0	41.0
50-51	33.06273149290113	37.0	32.5	39.5	18.0	40.0
52-53	33.18223189785546	37.5	33.0	39.5	16.5	40.0
54-55	34.10680159916938	39.0	34.0	40.5	19.0	41.0
56-57	34.13589983007927	39.0	34.0	41.0	18.5	41.0
58-59	33.94772609732168	38.5	34.0	41.0	17.5	41.0
60-61	33.700333470659515	38.0	34.0	40.0	14.0	41.0
62-63	33.41278567739107	37.5	34.0	40.0	13.0	41.0
64-65	33.123366828506825	37.0	33.0	40.0	9.5	41.0
66-67	32.78502528228371	36.5	33.0	39.5	7.5	41.0
68-69	32.42331220296134	36.0	33.0	39.0	6.0	41.0
70-71	32.00907251247125	35.0	32.0	39.0	2.0	40.5
72-73	31.64077153355665	35.0	32.0	37.5	2.0	40.0
74-75	31.23372643905805	35.0	32.0	37.0	2.0	39.0
76-77	30.849926931798763	35.0	31.5	37.0	2.0	39.0
78-79	30.499260496876836	35.0	31.0	36.0	2.0	38.5
80-81	30.176490988255182	35.0	31.0	36.0	2.0	37.0
82-83	29.860121522236224	35.0	31.0	35.0	2.0	37.0
84-85	29.596402979705893	35.0	31.0	35.0	2.0	36.5
86-87	29.35761632676531	35.0	31.0	35.0	2.0	36.0
88-89	29.132785814608347	35.0	30.0	35.0	2.0	36.0
90-91	28.90632143670412	34.0	30.0	35.0	2.0	35.5
92-93	28.714858899798386	34.0	30.0	35.0	2.0	35.0
94-95	28.483013154236467	34.0	30.0	35.0	2.0	35.0
96-97	28.242946133396757	34.0	29.5	35.0	2.0	35.0
98-99	27.976962035572598	34.0	29.0	35.0	2.0	35.0
100-101	26.900599639510602	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	107272.0
3	18310.0
4	12559.0
5	9596.0
6	8702.0
7	8919.0
8	9302.0
9	10135.0
10	10970.0
11	11741.0
12	11860.0
13	12095.0
14	12950.0
15	13745.0
16	13709.0
17	14236.0
18	13468.0
19	12952.0
20	13018.0
21	13896.0
22	15287.0
23	17260.0
24	20189.0
25	23651.0
26	28188.0
27	33229.0
28	38581.0
29	46014.0
30	54642.0
31	66453.0
32	82472.0
33	107293.0
34	148011.0
35	214239.0
36	353078.0
37	644177.0
38	707983.0
39	120644.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.690267066155606	18.061794392205652	13.193464263369814	25.05447427826893
2	15.908008997698264	19.786500836995188	39.409754917346724	24.895735247959824
3	18.77172517151279	23.921326435332553	29.57127687120913	27.73567152194553
4	13.365763678057501	15.955886535859845	36.51702805609519	34.16132172998746
5	14.320354228795868	37.092186710273985	34.3908782946593	14.19658076627085
6	33.41869992282373	35.195845420035646	17.332412794402007	14.053041862738628
7	28.805607064378314	31.749381213393562	21.286161235776078	18.158850486452042
8	26.308397716831116	34.739836643893355	20.22188616309208	18.72987947618344
9	26.918770389901475	15.4347204592496	19.38395338237528	38.26255576847364
10-11	25.440322830979355	25.11495952359558	28.246289322561406	21.198428322863656
12-13	26.064473794715052	22.746850049033913	27.80312712511605	23.385549031134982
14-15	23.408238598665577	24.27657162803741	25.01679959262622	27.298390180670793
16-17	23.538323755138297	27.188174073203214	25.890914295919735	23.382587875738746
18-19	23.517217606100903	26.74953357751762	27.056865967659192	22.67638284872228
20-21	24.19965381426812	25.968292341008304	26.776587335431884	23.05546650929169
22-23	24.04826772960868	25.7607975202668	26.422205691829614	23.76872905829491
24-25	23.810357990733866	25.938673300772873	26.48043431084431	23.770534397648955
26-27	23.61998955609482	26.10278619600687	26.52833611859257	23.748888129305744
28-29	23.764276072855882	26.31293471914125	26.334826903076113	23.587962304926755
30-31	24.094284112281454	26.022643060567525	26.23931040251571	23.643762424635316
32-33	23.827764375146113	26.109885750983114	26.087618522348016	23.974731351522756
34-35	24.088321487574817	26.149837967928153	26.188849167965273	23.572991376531757
36-37	24.122006720432303	26.05804336426736	26.187437006156856	23.63251290914348
38-39	23.957014710420403	26.122027110274487	26.180314020730076	23.740644158575027
40-41	24.109601080243635	25.97075396523317	26.204321039888057	23.71532391463514
42-43	23.9393461095249	25.920056402728054	26.097788524481185	24.04280896326586
44-45	23.878427880696492	26.004851492603276	26.222808562458876	23.893912064241356
46-47	24.03956488189497	25.92281881941132	26.00515562638667	24.03246067230704
48-49	23.80096755520748	26.01180435687962	26.121338599795173	24.06588948811772
50-51	23.86220501891994	26.048118211938366	26.19047813914934	23.89919862999236
52-53	24.028515316718714	25.918421167652717	26.156507168362847	23.89655634726572
54-55	23.634568952111245	25.76374008626687	26.419319195606445	24.182371766015436
56-57	23.71741952825443	25.878676831087947	26.270147504591357	24.13375613606626
58-59	23.825892262942507	25.80393831257227	26.33814270084112	24.0320267236441
60-61	23.903397720554516	25.729347733726005	26.39249010582871	23.97476443989077
62-63	23.867824966413423	25.73101042604004	26.42371160870339	23.97745299884315
64-65	23.666484634665704	25.56634542602036	26.426774026259753	24.340395913054184
66-67	23.669858518417026	25.76010481596558	26.27018888383077	24.29984778178662
68-69	23.670545446212422	25.699678032380092	26.32505143788742	24.304725083520072
70-71	23.5611475761058	25.509978449436378	26.356403145953472	24.572470828504358
72-73	23.551443510737016	25.636562773004663	26.295170531693792	24.516823184564533
74-75	23.507600843974405	25.659571890872602	26.342346530391097	24.490480734761892
76-77	23.519263794201777	25.377324458114742	26.457674124442924	24.645737623240553
78-79	23.449261952447888	25.372412544401868	26.46955494503156	24.708770558118687
80-81	23.61984038037574	25.49684862168643	26.295126845126067	24.588184152811763
82-83	23.512973476724106	25.5174949882869	26.41140057488164	24.558130960107352
84-85	23.494598024667425	25.48068674305877	26.43369433817453	24.59102089409928
86-87	23.426626159009857	25.56647648067434	26.378976716066553	24.627920644249247
88-89	23.39336321236974	25.2528289133711	26.46907991299581	24.884727961263355
90-91	23.33297199259983	25.241227689529495	26.531672606955492	24.894127710915186
92-93	23.415314513717803	25.108863772343803	26.659171656153895	24.8166500577845
94-95	23.314333154302634	25.05364371143744	26.645663308749363	24.986359825510565
96-97	23.292453463598683	24.9445427912179	26.660694897740278	25.10230884744314
98-99	23.152047025896632	24.822640104288027	26.680823254487972	25.344489615327365
100-101	23.35974123336651	24.68979806128087	26.756429945373018	25.1940307599796
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	62.5
2	85.5
3	125.5
4	117.5
5	131.5
6	204.0
7	420.0
8	651.0
9	722.0
10	628.5
11	571.0
12	678.5
13	736.5
14	804.0
15	940.0
16	1053.0
17	1150.0
18	1265.5
19	1362.0
20	1453.5
21	1560.5
22	1668.0
23	1873.0
24	2137.0
25	2410.5
26	2821.5
27	3532.5
28	4606.5
29	6299.0
30	8952.0
31	13062.0
32	19361.5
33	26030.5
34	32849.0
35	41153.5
36	51372.5
37	61524.0
38	71322.5
39	80551.5
40	88278.5
41	96476.5
42	104196.0
43	112268.0
44	120747.0
45	128662.5
46	133206.5
47	136104.5
48	140185.5
49	141762.0
50	139510.0
51	135688.5
52	132528.0
53	129443.0
54	125543.5
55	118529.0
56	108161.5
57	96161.0
58	85578.0
59	75092.0
60	61404.0
61	49624.5
62	40515.5
63	30587.0
64	21846.0
65	15914.0
66	11998.5
67	9230.0
68	6934.0
69	5127.5
70	3729.5
71	2667.0
72	1882.0
73	1334.5
74	946.0
75	654.5
76	489.5
77	332.0
78	215.5
79	156.5
80	128.5
81	104.0
82	88.0
83	71.0
84	58.5
85	53.0
86	48.0
87	48.5
88	38.0
89	28.5
90	29.0
91	26.0
92	22.5
93	22.0
94	17.5
95	17.5
96	14.5
97	7.0
98	6.5
99	7.0
100	6.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.052175236920334586
2	0.07445670941856139
3	0.07105894821648574
4	0.07125497290122088
5	0.06615833109810741
6	0.010225954387016109
7	0.013525703246724183
8	0.017348184599059277
9	0.006338131473102636
10-11	0.013525703246724183
12-13	0.02326159592190246
14-15	0.04054443895938336
16-17	0.027786499061205117
18-19	0.010095271263859354
20-21	0.02064793345876735
22-23	0.03773475181151312
24-25	0.03577450496416179
26-27	0.022248801717437604
28-29	0.012610921384626893
30-31	0.012643592165416084
32-33	0.009392849476891793
34-35	0.005864405151659398
36-37	7.840987389405322E-4
38-39	0.0038388167427296895
40-41	0.0015681974778810645
42-43	0.23150515267219215
44-45	0.19958579984115468
46-47	0.20621796834136
48-49	0.21376491870366263
50-51	0.2044374107883492
52-53	0.21172299490433832
54-55	0.20791684894239781
56-57	0.21103690850776535
58-59	0.22100149664846794
60-61	0.22524869815106252
62-63	0.2097300772761978
64-65	0.21010579125527346
66-67	0.20721442715543026
68-69	0.20203610840034383
70-71	0.19375406547028445
72-73	0.20548287577360327
74-75	0.20399635524769513
76-77	0.19736418674748982
78-79	0.2166726181939004
80-81	0.22923453340734354
82-83	0.2284667700587976
84-85	0.22121385672359767
86-87	0.2425642119693326
88-89	0.23500092621663538
90-91	0.2416330947168407
92-93	0.2369285022831975
94-95	0.24042427582764073
96-97	0.2477752015052082
98-99	0.2408653313682948
100-101	0.2478405430667866
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3060839.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.91768844683475
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.54722109690319	50.6784496223327
2	11.008075962164398	13.852053875731007
3	3.4542697048292137	6.52003995299353
4	1.5932227932739216	4.009675813344176
5	0.8741619018758926	2.7500123097159968
6	0.5433084238737921	2.051022608629925
7	0.3684716100097757	1.6228367372069064
8	0.2724416652304713	1.3713119850326119
9	0.21235350125368663	1.2024712289226587
>10	1.062318287285457	11.631070959272618
>50	0.04671669126007203	1.9680511570018049
>100	0.01629251169989069	1.7376741712963635
>500	7.812615959024026E-4	0.32042619214082385
>1k	3.645887443605859E-4	0.2849033863789631
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	3.267078078918885E-5	0.0
6	0.0	0.0	0.0	3.267078078918885E-5	3.267078078918885E-5
7	0.0	0.0	0.0	3.267078078918885E-5	3.267078078918885E-5
8	0.0	0.0	0.0	3.267078078918885E-5	3.267078078918885E-5
9	0.0	0.0	0.0	6.53415615783777E-5	6.53415615783777E-5
10-11	0.0	0.0	0.0	6.53415615783777E-5	6.53415615783777E-5
12-13	0.0	0.0	0.0	6.53415615783777E-5	1.306831231567554E-4
14-15	0.0	0.0	0.0	6.53415615783777E-5	1.7968929434053867E-4
16-17	3.267078078918885E-5	0.0	0.0	6.53415615783777E-5	2.2869546552432192E-4
18-19	3.267078078918885E-5	0.0	0.0	6.53415615783777E-5	2.777016367081052E-4
20-21	3.267078078918885E-5	0.0	0.0	6.53415615783777E-5	3.4304319828648284E-4
22-23	3.267078078918885E-5	0.0	0.0	1.470185135513498E-4	4.24720150259455E-4
24-25	3.267078078918885E-5	0.0	0.0	3.7571397907567173E-4	4.900617118378327E-4
26-27	3.267078078918885E-5	0.0	0.0	7.187571773621547E-4	4.900617118378327E-4
28-29	4.900617118378327E-5	0.0	0.0	0.002744345586291863	4.900617118378327E-4
30-31	6.53415615783777E-5	0.0	0.0	0.012137195063183656	5.063971022324272E-4
32-33	6.53415615783777E-5	1.6335390394594424E-5	0.0	0.036035871210475294	5.554032734162103E-4
34-35	6.53415615783777E-5	3.267078078918885E-5	0.0	0.06976845237531278	6.207448349945881E-4
36-37	6.53415615783777E-5	3.267078078918885E-5	0.0	0.12481871800509597	6.207448349945881E-4
38-39	6.53415615783777E-5	3.267078078918885E-5	0.0	0.21489206064088964	7.187571773621547E-4
40-41	9.801234236756654E-5	3.267078078918885E-5	0.0	0.31615514569698044	8.4944030051891E-4
42-43	9.801234236756654E-5	3.267078078918885E-5	0.0	0.4219431338923739	8.984464717026933E-4
44-45	9.801234236756654E-5	3.267078078918885E-5	0.0	0.5561873721551509	9.801234236756654E-4
46-47	9.801234236756654E-5	3.267078078918885E-5	0.0	0.711291903951825	0.0010618003756486375
48-49	9.801234236756654E-5	3.267078078918885E-5	0.0	0.8690264336020288	0.0011108065468324207
50-51	9.801234236756654E-5	3.267078078918885E-5	0.0	1.0363498374138596	0.0011108065468324207
52-53	9.801234236756654E-5	3.267078078918885E-5	0.0	1.2070383316469766	0.0011924834988053929
54-55	9.801234236756654E-5	3.267078078918885E-5	0.0	1.409009098485742	0.0013231666219621482
56-57	9.801234236756654E-5	3.267078078918885E-5	0.0	1.6464603332615666	0.001388508183540526
58-59	9.801234236756654E-5	3.267078078918885E-5	0.0	1.892651001898499	0.0014375143547243092
60-61	9.801234236756654E-5	3.267078078918885E-5	0.0	2.146012906918658	0.0014375143547243092
62-63	9.801234236756654E-5	3.267078078918885E-5	0.0	2.410009151085699	0.001502855916302687
64-65	9.801234236756654E-5	3.267078078918885E-5	0.0	2.7022819560257823	0.0015191913066972813
66-67	9.801234236756654E-5	3.267078078918885E-5	0.0	3.0402121771187574	0.00155186208748647
68-69	9.801234236756654E-5	3.267078078918885E-5	0.0	3.3976631897332723	0.0015681974778810647
70-71	9.801234236756654E-5	3.267078078918885E-5	0.0	3.7758274773681335	0.0016662098202486313
72-73	9.801234236756654E-5	3.267078078918885E-5	0.0	4.171519638896394	0.0017968929434053864
74-75	9.801234236756654E-5	3.267078078918885E-5	0.0	4.593054388028904	0.0018949052857729532
76-77	9.801234236756654E-5	3.267078078918885E-5	0.0	5.0635299667836176	0.0018949052857729532
78-79	9.801234236756654E-5	3.267078078918885E-5	0.0	5.580071346451088	0.0018949052857729532
80-81	9.801234236756654E-5	3.267078078918885E-5	0.0	6.123092393948196	0.0019439114569567363
82-83	9.801234236756654E-5	3.267078078918885E-5	0.0	6.679802498595974	0.002041923799324303
84-85	9.801234236756654E-5	3.267078078918885E-5	0.0	7.255526997663059	0.0020909299705080863
86-87	9.801234236756654E-5	3.267078078918885E-5	0.0	7.886171079236771	0.0020909299705080863
88-89	9.801234236756654E-5	3.267078078918885E-5	0.0	8.564416488420331	0.0020909299705080863
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2410	0.0	49.43259	1
GTATCAA	4265	0.0	42.065826	1
TCAACGC	5170	0.0	33.60281	4
CAACGCA	5275	0.0	32.84181	5
TATCAAC	5405	0.0	32.406338	2
ATCAACG	5365	0.0	32.38146	3
GTACATG	8425	0.0	30.5905	1
AACGCAG	5755	0.0	30.086382	6
TACATGG	8735	0.0	28.746967	2
ACATGGG	8795	0.0	27.848316	3
ACGCAGA	6750	0.0	25.092073	7
CATGGGG	7735	0.0	24.791689	4
CGCAGAG	6745	0.0	24.549974	8
ATGGGGG	4450	0.0	19.838537	5
AATACCG	290	2.4665496E-9	19.63993	5
GCAGAGT	8020	0.0	19.583467	9
GTATATA	830	0.0	19.442741	1
TAAGGCG	670	0.0	19.126945	5
AGAGTAC	6645	0.0	18.601835	10-11
ATACCGT	305	9.430005E-8	17.108631	6
>>END_MODULE