FastQCFastQC Report
Mon 15 Aug 2016
SRR2921685_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921685_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3060839
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26000.049.9185831
GTATCAA44800.039.370311
TCAACGC57000.030.4762614
CAACGCA58700.029.6159115
ATCAACG59800.029.1281743
TATCAAC60200.029.0134742
AACGCAG64150.027.2425676
GTACATG82950.026.249561
TACATGG84250.025.0690862
ACATGGG87850.023.123333
ACGCAGA74650.022.9646637
CGCAGAG77550.021.9834238
CATGGGG74450.021.2292674
TAAGGCG6900.019.963245
ATGGGGG42750.018.777285
AGAGTAC74950.018.34314510-11
GCAGAGT91050.017.6813989
TCTATAC9050.016.7822343
CTACACG6100.016.3397334
TATACCG2355.140019E-516.1697855
Produced by FastQC (version 0.11.5)