##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921685_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3060839
Sequences flagged as poor quality	0
Sequence length	101
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.303778473810613	33.0	31.0	34.0	28.0	34.0
2	31.438074658614845	34.0	31.0	34.0	28.0	34.0
3	31.507046924062323	34.0	31.0	34.0	28.0	34.0
4	34.98974823569616	37.0	35.0	37.0	32.0	37.0
5	34.721535827268276	37.0	35.0	37.0	32.0	37.0
6	34.69187565892881	37.0	35.0	37.0	32.0	37.0
7	34.574084752579274	37.0	35.0	37.0	32.0	37.0
8	34.51682496204472	37.0	35.0	37.0	31.0	37.0
9	36.09883629945907	39.0	37.0	39.0	32.0	39.0
10-11	35.983798559806644	39.0	37.0	39.0	31.0	39.0
12-13	35.90766779958044	39.0	37.0	39.0	30.5	39.0
14-15	37.13277437983507	40.0	38.0	41.0	31.0	41.0
16-17	37.10028818895734	40.0	38.0	41.0	31.0	41.0
18-19	37.06376372622016	40.0	38.0	41.0	31.0	41.0
20-21	37.00471227006713	40.0	37.5	41.0	31.0	41.0
22-23	36.91732070847242	40.0	37.0	41.0	30.0	41.0
24-25	36.83142644876127	40.0	37.0	41.0	30.0	41.0
26-27	36.703182689452134	40.0	37.0	41.0	30.0	41.0
28-29	36.58120240888201	40.0	37.0	41.0	30.0	41.0
30-31	36.42242600803244	40.0	37.0	41.0	30.0	41.0
32-33	36.36631230848796	40.0	36.5	41.0	29.5	41.0
34-35	36.49348005563181	40.0	37.0	41.0	30.0	41.0
36-37	36.489507778749555	40.0	37.0	41.0	30.0	41.0
38-39	36.388538240658846	40.0	37.0	41.0	29.5	41.0
40-41	36.30660024914738	40.0	37.0	41.0	29.0	41.0
42-43	36.18544898963977	40.0	36.0	41.0	29.0	41.0
44-45	36.056663712139056	40.0	36.0	41.0	28.0	41.0
46-47	35.901022236060115	40.0	36.0	41.0	27.5	41.0
48-49	35.7596853346419	40.0	35.5	41.0	27.0	41.0
50-51	35.604358478181965	40.0	35.0	41.0	27.0	41.0
52-53	35.43816532003153	39.0	35.0	41.0	26.5	41.0
54-55	35.22991816949536	39.0	35.0	41.0	26.0	41.0
56-57	35.00790159168777	39.0	35.0	41.0	26.0	41.0
58-59	34.77042961096615	38.5	35.0	40.5	26.0	41.0
60-61	34.49701405398977	38.0	34.0	40.0	25.0	41.0
62-63	34.19450206299645	37.5	34.0	40.0	24.0	41.0
64-65	33.86226782918017	37.0	34.0	40.0	23.0	41.0
66-67	33.51481358542543	36.5	34.0	39.5	22.5	41.0
68-69	33.14886849651354	36.0	33.5	39.0	22.0	41.0
70-71	32.75112951056883	35.5	33.0	39.0	21.5	40.0
72-73	32.384164766588505	35.0	33.0	37.5	20.0	40.0
74-75	31.975767591826944	35.0	33.0	37.0	19.5	39.0
76-77	29.264666648588836	33.0	29.0	34.5	12.0	36.5
78-79	30.83019933423483	34.5	31.5	36.0	14.0	37.0
80-81	31.009265106724005	35.0	32.0	36.0	16.5	37.0
82-83	30.82919307418652	35.0	32.0	35.0	13.0	37.0
84-85	30.62927239884228	35.0	32.0	35.0	10.5	36.0
86-87	30.421695162666182	35.0	32.0	35.0	7.0	36.0
88-89	30.153156046430407	35.0	31.5	35.0	2.0	36.0
90-91	29.95635575735934	34.0	31.0	35.0	2.0	35.0
92-93	29.79829893045665	34.0	31.0	35.0	2.0	35.0
94-95	29.69342066015233	34.0	31.0	35.0	2.0	35.0
96-97	29.49332388929963	34.0	31.0	35.0	2.0	35.0
98-99	29.295883579632903	34.0	31.0	35.0	2.0	35.0
100-101	28.184872677066647	33.0	28.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	61514.0
3	20966.0
4	11333.0
5	7728.0
6	6663.0
7	6835.0
8	7040.0
9	7489.0
10	8548.0
11	9384.0
12	9653.0
13	10613.0
14	11438.0
15	11933.0
16	12742.0
17	13773.0
18	13145.0
19	11707.0
20	11561.0
21	11259.0
22	11863.0
23	13511.0
24	15793.0
25	18982.0
26	22927.0
27	27792.0
28	33007.0
29	39830.0
30	48753.0
31	60389.0
32	76544.0
33	100728.0
34	143914.0
35	212944.0
36	370897.0
37	724556.0
38	781479.0
39	91605.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.489130968346196	19.757924906062218	13.765640207006735	25.987303918584857
2	14.979863298472406	21.240226095610044	37.89545250649415	25.88445809942339
3	17.35128142879875	25.167969328178234	29.435270830630916	28.045478412392104
4	13.00480912960276	17.264216566682034	35.605659867537376	34.12531443617783
5	14.088410518441979	37.5109922483204	34.44051539306908	13.960081840168542
6	33.31032177735029	35.85235271651255	17.089363793097252	13.747961713039908
7	28.53953721170477	31.99006285206862	21.423283427610347	18.047116508616266
8	26.278513832318524	35.28245033469582	20.018465525302048	18.42057030768361
9	26.74960255982185	15.37931769012277	19.368290644588182	38.502789105467194
10-11	25.319540276473475	25.45846017264638	28.2525315290962	20.969468021783946
12-13	25.87709561453418	22.97163854272694	27.88349813359235	23.267767709146533
14-15	23.224910383579353	24.51057092312877	25.05562891426049	27.20888977903138
16-17	23.424316572076574	27.514012908554307	25.86655584736013	23.195114672008994
18-19	23.43111250799017	26.988019275406376	27.037516276427194	22.543351940176258
20-21	24.07096701369122	26.138069389990726	26.82925434779874	22.961709248519316
22-23	23.903564333760176	25.94919660086607	26.50522070994598	23.64201835542777
24-25	23.658994537083046	26.147658518847404	26.510747613628897	23.68259933044064
26-27	23.525435424181843	26.344253420157344	26.527786807878194	23.60252434778262
28-29	23.658928299253247	26.531218690356994	26.393976982404276	23.41587602798548
30-31	24.02278074707296	26.182566533025238	26.232659603859553	23.56199311604225
32-33	23.744283956849692	26.255912091789973	26.125964518463697	23.873839432896634
34-35	24.006519138697353	26.28566910208601	26.258304533827015	23.449507225389617
36-37	24.012625791521536	26.199840625683052	26.2530960862464	23.534437496549014
38-39	23.90568402977092	26.247231559712876	26.19961389671263	23.64747051380357
40-41	24.014352843736802	26.14047196449852	26.208674926712444	23.63650026505223
42-43	23.902016416311625	26.062360942228256	26.07260418744518	23.963018454014936
44-45	23.789002743890286	26.10864864546964	26.17280235560269	23.92954625503738
46-47	23.93285486094253	26.04002727469057	26.027105448959116	24.00001241540778
48-49	23.697631406591572	26.146178300192823	26.142110376652145	24.014079916563457
50-51	23.833707152997064	26.08931812352875	26.12453830685013	23.952436416624057
52-53	23.99169947160338	26.047784069951046	26.05332189394234	23.907194564503236
54-55	23.583497524190822	25.930447984973455	26.285299536889482	24.20075495394624
56-57	23.61436551909475	25.99009832246534	26.140101957382434	24.255434201057476
58-59	23.68175381018013	25.975230316492798	26.22415576760074	24.11886010572633
60-61	23.771590238362343	25.925112726582434	26.23673325625728	24.066563778797946
62-63	23.743737908184798	25.908316816056054	26.247136810111954	24.100808465647194
64-65	23.501666807480674	25.76026897708403	26.22502056238553	24.513043653049763
66-67	23.54018911563514	26.002680636487714	26.033475265407528	24.423654982469618
68-69	23.55135564843162	25.925284887852477	26.044201990406872	24.479157473309026
70-71	23.44783382882449	25.692836239123213	26.08459764357951	24.77473228847279
72-73	23.387702183719806	25.85392605152202	26.046113134314602	24.71225863044357
74-75	23.359501698125076	25.873200669609485	26.013053953563613	24.754243678701826
76-77	23.26223061825978	25.557715144778438	26.254989035868665	24.925065201093123
78-79	23.14242603440978	25.61207391400809	26.26640675593509	24.97909329564704
80-81	23.30216955354183	25.741617752925507	26.054928327774483	24.90128436575818
82-83	23.174159713057087	25.69474684732571	26.18831192280084	24.94278151681636
84-85	23.122211843886955	25.659003859596652	26.154140342877312	25.064643953639077
86-87	23.01207428797009	25.791228344049777	26.121932899553663	25.07476446842647
88-89	22.90462438399736	25.55855357115649	26.165090592261997	25.37173145258416
90-91	22.8442085826144	25.454694403187737	26.239226265424843	25.46187074877302
92-93	22.836858312681084	25.363523454979347	26.349421521804544	25.450196710535028
94-95	22.63293997984358	25.322265001199945	26.38429165086756	25.660503368088918
96-97	22.55308435513988	25.20492087850938	26.4221029454267	25.819891820924042
98-99	22.34063302789161	25.118146714614713	26.402805398434992	26.138414859058688
100-101	22.27368244973916	25.11761507982143	26.420969853948208	26.187732616491203
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	71.0
1	65.5
2	81.5
3	105.0
4	100.0
5	111.5
6	174.5
7	385.0
8	562.5
9	583.5
10	513.5
11	438.5
12	445.5
13	461.5
14	488.0
15	546.5
16	621.5
17	653.5
18	702.5
19	811.5
20	922.0
21	1035.5
22	1216.5
23	1492.5
24	1792.5
25	2197.0
26	2816.5
27	3662.5
28	4937.5
29	6975.5
30	10236.0
31	14803.0
32	21098.5
33	27779.5
34	34528.0
35	42571.0
36	52080.0
37	62039.0
38	71777.5
39	80744.0
40	88640.0
41	96461.5
42	104617.5
43	112790.5
44	120591.5
45	128860.5
46	133781.5
47	137236.0
48	141506.0
49	142397.5
50	140194.0
51	136799.0
52	133768.5
53	130188.5
54	125846.5
55	118206.5
56	107185.0
57	95072.5
58	84216.0
59	73861.5
60	60618.5
61	48801.0
62	39464.0
63	29801.5
64	21058.0
65	15066.0
66	11285.0
67	8652.0
68	6396.0
69	4604.0
70	3347.5
71	2344.0
72	1566.0
73	1051.5
74	758.5
75	505.5
76	299.0
77	163.5
78	90.0
79	52.5
80	32.5
81	22.0
82	15.5
83	11.5
84	5.5
85	3.5
86	2.5
87	3.0
88	2.5
89	1.5
90	1.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.1663922865593388
2	3.2670780789188845E-4
3	0.010127942044648543
4	0.006599497719416147
5	0.02378432841452948
6	0.001894905285772953
7	3.267078078918885E-5
8	0.0
9	3.267078078918885E-5
10-11	0.04972492836114543
12-13	4.083847598648606E-4
14-15	0.005080306412718866
16-17	1.6335390394594424E-5
18-19	0.00155186208748647
20-21	2.777016367081052E-4
22-23	1.6335390394594424E-5
24-25	6.534156157837769E-4
26-27	0.0028750287094486187
28-29	0.005897075932448587
30-31	0.017495203112610627
32-33	0.01221887201515663
34-35	0.01019328360622692
36-37	0.0039204936947026614
38-39	0.0
40-41	0.003969499865886445
42-43	0.009327507915313416
44-45	0.007220242554410735
46-47	0.004116518379437795
48-49	0.010111606654253948
50-51	0.003071053394183752
52-53	0.0022869546552432194
54-55	0.004002170646675634
56-57	0.008053347464535052
58-59	0.01584532868275659
60-61	0.0073672610679620856
62-63	0.021546379930470042
64-65	0.04075679903451308
66-67	0.06089833539104801
68-69	0.051129771935080545
70-71	0.06826559645901009
72-73	0.034631027636540176
74-75	0.027655815938048358
76-77	0.22926720418813273
78-79	0.08525440246938829
80-81	0.0670731129602047
82-83	0.028015194526729438
84-85	0.051668839818102164
86-87	0.06751416850085874
88-89	0.2942167163970401
90-91	0.2988232964883158
92-93	0.05385778213097782
94-95	0.009000800107421526
96-97	0.014718186745529575
98-99	8.494403005189099E-4
100-101	2.2869546552432195E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3060839.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.112776591377695
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.97321740089934	49.673585857157406
2	11.318724207816153	14.060747760390063
3	3.5264062347175336	6.571046478823547
4	1.6521072664880707	4.10467878193461
5	0.8997121003587252	2.794180834307034
6	0.573575448555481	2.137581821665551
7	0.37751691032635104	1.6414036457397467
8	0.28845614649096124	1.4333449746722235
9	0.21928087647656125	1.2258129682215122
>10	1.1049292326415936	12.002384672945116
>50	0.04883236869588781	2.0579875090049713
>100	0.01597483340023174	1.6788970110584722
>500	0.0010030203976826538	0.3954762659988763
>1k	2.6395273544048664E-4	0.2228714180808098
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	3.267078078918885E-5
3	0.0	0.0	0.0	0.0	3.267078078918885E-5
4	0.0	0.0	0.0	3.267078078918885E-5	6.53415615783777E-5
5	0.0	0.0	0.0	6.53415615783777E-5	6.53415615783777E-5
6	0.0	0.0	0.0	6.53415615783777E-5	1.306831231567554E-4
7	0.0	0.0	0.0	9.801234236756654E-5	1.306831231567554E-4
8	0.0	0.0	0.0	9.801234236756654E-5	1.306831231567554E-4
9	0.0	0.0	0.0	9.801234236756654E-5	1.960246847351331E-4
10-11	0.0	0.0	0.0	9.801234236756654E-5	1.960246847351331E-4
12-13	0.0	0.0	0.0	9.801234236756654E-5	2.2869546552432192E-4
14-15	0.0	0.0	0.0	9.801234236756654E-5	3.1037241749729406E-4
16-17	0.0	0.0	0.0	9.801234236756654E-5	4.24720150259455E-4
18-19	0.0	0.0	0.0	9.801234236756654E-5	4.737263214432383E-4
20-21	0.0	0.0	0.0	9.801234236756654E-5	4.900617118378327E-4
22-23	0.0	0.0	0.0	1.7968929434053867E-4	5.063971022324272E-4
24-25	0.0	0.0	0.0	4.0838475986486057E-4	5.554032734162103E-4
26-27	0.0	0.0	0.0	7.514279581513435E-4	5.554032734162103E-4
28-29	0.0	0.0	0.0	0.0027606809766864576	5.554032734162103E-4
30-31	0.0	0.0	0.0	0.012578250603837706	5.717386638108047E-4
32-33	0.0	0.0	0.0	0.03711400697651853	6.207448349945881E-4
34-35	0.0	0.0	0.0	0.0714673329763506	6.207448349945881E-4
36-37	0.0	0.0	0.0	0.12723635578349596	6.207448349945881E-4
38-39	0.0	0.0	0.0	0.21897590823953825	6.207448349945881E-4
40-41	0.0	0.0	0.0	0.3212191167193047	6.207448349945881E-4
42-43	0.0	0.0	0.0	0.42746449584574686	6.370802253891825E-4
44-45	0.0	0.0	0.0	0.562950223778513	6.534156157837769E-4
46-47	0.0	0.0	0.0	0.7202763686688518	6.534156157837769E-4
48-49	0.0	0.0	3.267078078918885E-5	0.8788276678387854	7.514279581513435E-4
50-51	0.0	0.0	3.267078078918885E-5	1.0458080284523297	7.840987389405324E-4
52-53	0.0	0.0	3.267078078918885E-5	1.216088137925582	8.167695197297211E-4
54-55	0.0	0.0	3.267078078918885E-5	1.4184509541338177	8.821110813080988E-4
56-57	0.0	0.0	3.267078078918885E-5	1.656833306162134	9.311172524918822E-4
58-59	0.0	0.0	3.267078078918885E-5	1.9030893163606448	9.474526428864766E-4
60-61	0.0	0.0	3.267078078918885E-5	2.1569739538734316	9.474526428864766E-4
62-63	0.0	3.267078078918885E-5	3.267078078918885E-5	2.4205781486710016	9.801234236756654E-4
64-65	0.0	4.900617118378327E-5	3.267078078918885E-5	2.71365138774042	9.801234236756654E-4
66-67	0.0	9.801234236756654E-5	3.267078078918885E-5	3.051793968908525	9.801234236756654E-4
68-69	0.0	9.801234236756654E-5	3.267078078918885E-5	3.4111398868088125	9.801234236756654E-4
70-71	0.0	9.801234236756654E-5	3.267078078918885E-5	3.7899902608402467	9.801234236756654E-4
72-73	0.0	9.801234236756654E-5	3.267078078918885E-5	4.188524780297167	0.0011924834988053929
74-75	0.0	9.801234236756654E-5	3.267078078918885E-5	4.613114247433465	0.0012088188891999873
76-77	0.0	9.801234236756654E-5	3.267078078918885E-5	5.086775227315125	0.0012088188891999873
78-79	0.0	9.801234236756654E-5	3.267078078918885E-5	5.607318777629271	0.0012088188891999873
80-81	0.0	9.801234236756654E-5	3.267078078918885E-5	6.155518143881465	0.0012088188891999873
82-83	0.0	9.801234236756654E-5	3.267078078918885E-5	6.716328431518287	0.0013068312315675538
84-85	0.0	9.801234236756654E-5	3.267078078918885E-5	7.297982677298609	0.0013231666219621482
86-87	0.0	9.801234236756654E-5	3.267078078918885E-5	7.93516091503016	0.0013721727931459316
88-89	0.0	9.801234236756654E-5	3.267078078918885E-5	8.618404953674467	0.0014211789643297148
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2600	0.0	49.918583	1
GTATCAA	4480	0.0	39.37031	1
TCAACGC	5700	0.0	30.476261	4
CAACGCA	5870	0.0	29.615911	5
ATCAACG	5980	0.0	29.128174	3
TATCAAC	6020	0.0	29.013474	2
AACGCAG	6415	0.0	27.242567	6
GTACATG	8295	0.0	26.24956	1
TACATGG	8425	0.0	25.069086	2
ACATGGG	8785	0.0	23.12333	3
ACGCAGA	7465	0.0	22.964663	7
CGCAGAG	7755	0.0	21.983423	8
CATGGGG	7445	0.0	21.229267	4
TAAGGCG	690	0.0	19.96324	5
ATGGGGG	4275	0.0	18.77728	5
AGAGTAC	7495	0.0	18.343145	10-11
GCAGAGT	9105	0.0	17.681398	9
TCTATAC	905	0.0	16.782234	3
CTACACG	610	0.0	16.339733	4
TATACCG	235	5.140019E-5	16.169785	5
>>END_MODULE