FastQCFastQC Report
Mon 15 Aug 2016
SRR2921684_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921684_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3584544
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA47450.046.013761
GGTATCA25400.042.045061
TCAACGC57750.037.0683144
CAACGCA58850.036.452035
ATCAACG61450.034.9131283
TATCAAC63650.034.0051542
AACGCAG64700.033.3571056
ACGCAGA74650.028.6608167
GTACATG107150.028.438651
CGCAGAG77400.027.0927738
TACATGG113550.026.3767032
ACATGGG117350.024.8346163
CATGGGG99500.023.2795734
GCAGAGT92550.021.1718069
AGAGTAC76000.019.2586510-11
GAGTACA81700.018.9392011
GTATATA7250.018.3311
AGTACAT80650.018.0092372
ATGGGGG57700.017.9312445
GTATTAA16750.017.00211
Produced by FastQC (version 0.11.5)