##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921684_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3584544
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.214478326950374	31.0	31.0	34.0	26.0	34.0
2	30.27215009775302	31.0	31.0	34.0	26.0	34.0
3	30.33503815269111	31.0	31.0	34.0	26.0	34.0
4	33.784475235901695	37.0	35.0	37.0	28.0	37.0
5	33.7698265107082	37.0	35.0	37.0	28.0	37.0
6	33.81170603569101	37.0	35.0	37.0	29.0	37.0
7	33.722502499620596	37.0	35.0	37.0	29.0	37.0
8	33.68715100163368	37.0	35.0	37.0	28.0	37.0
9	35.223000749886175	39.0	35.0	39.0	29.0	39.0
10-11	35.11685349656749	39.0	35.0	39.0	28.0	39.0
12-13	35.00428590080077	39.0	35.0	39.0	27.0	39.0
14-15	36.12001986863601	40.0	36.0	41.0	27.0	41.0
16-17	36.05664988349983	40.0	36.0	41.0	27.0	41.0
18-19	36.01362265325798	40.0	36.0	41.0	27.0	41.0
20-21	35.93164221167323	40.0	36.0	41.0	27.0	41.0
22-23	35.82932975575136	40.0	36.0	41.0	27.0	41.0
24-25	35.712096155047895	40.0	36.0	41.0	26.5	41.0
26-27	35.55907543609452	40.0	36.0	41.0	25.5	41.0
28-29	35.41741501847933	40.0	36.0	41.0	25.0	41.0
30-31	35.26671969991162	39.0	35.0	41.0	25.0	41.0
32-33	35.092593507012324	39.0	35.0	41.0	24.0	41.0
34-35	34.91966676932965	39.0	35.0	41.0	24.0	41.0
36-37	34.89059110447521	39.0	35.0	41.0	23.5	41.0
38-39	35.07529479342421	39.5	35.0	41.0	24.0	41.0
40-41	35.136952147888266	40.0	35.0	41.0	24.0	41.0
42-43	34.976184976387515	40.0	35.0	41.0	23.0	41.0
44-45	34.85322107916656	40.0	35.0	41.0	22.0	41.0
46-47	34.69303180544024	39.0	35.0	41.0	21.5	41.0
48-49	34.523420831213116	39.0	35.0	41.0	20.0	41.0
50-51	32.82164732808413	37.0	32.0	39.5	16.0	40.0
52-53	32.93680479302249	37.5	32.5	39.5	14.5	40.0
54-55	33.83702097114724	38.0	34.0	40.0	16.0	41.0
56-57	33.84786265700742	38.0	34.0	41.0	14.5	41.0
58-59	33.64630884709464	38.0	34.0	40.0	13.0	41.0
60-61	33.38075470687485	37.5	33.0	40.0	12.0	41.0
62-63	33.07533775565316	37.0	33.0	40.0	8.0	41.0
64-65	32.77703007690797	36.5	33.0	40.0	7.5	41.0
66-67	32.4250682932055	36.0	33.0	39.0	4.0	41.0
68-69	32.05687613264058	35.5	32.0	39.0	2.0	41.0
70-71	31.645723138005838	35.0	32.0	38.5	2.0	40.0
72-73	31.2847610463144	35.0	32.0	37.0	2.0	39.5
74-75	30.892922363346635	35.0	31.5	37.0	2.0	39.0
76-77	30.52969136381085	35.0	31.0	36.0	2.0	39.0
78-79	30.201760809743163	35.0	31.0	36.0	2.0	37.5
80-81	29.903273331280072	35.0	31.0	35.0	2.0	37.0
82-83	29.612585310711765	35.0	31.0	35.0	2.0	36.5
84-85	29.377419554621174	35.0	30.0	35.0	2.0	36.0
86-87	29.162957268762774	34.5	30.0	35.0	2.0	36.0
88-89	28.961296471740894	34.0	30.0	35.0	2.0	35.5
90-91	28.75372976311631	34.0	30.0	35.0	2.0	35.0
92-93	28.577891636983672	34.0	30.0	35.0	2.0	35.0
94-95	28.36556741945419	34.0	29.5	35.0	2.0	35.0
96-97	28.14254393306373	34.0	29.0	35.0	2.0	35.0
98-99	27.88570331400591	34.0	29.0	35.0	2.0	35.0
100-101	26.826350436764063	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	133405.0
3	22713.0
4	15318.0
5	11976.0
6	10773.0
7	11316.0
8	11673.0
9	12644.0
10	13737.0
11	14304.0
12	14419.0
13	14412.0
14	14946.0
15	15456.0
16	15480.0
17	16360.0
18	15569.0
19	15343.0
20	15242.0
21	16238.0
22	17847.0
23	20388.0
24	23743.0
25	28470.0
26	33132.0
27	40112.0
28	46627.0
29	55567.0
30	66141.0
31	80774.0
32	100742.0
33	130410.0
34	180713.0
35	262507.0
36	437404.0
37	775460.0
38	766914.0
39	106263.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.502024305059315	17.345296421680718	13.020061052951265	25.132618220308707
2	16.155684543502723	19.253265879896027	38.839499348080395	25.751550228520852
3	19.448681855085024	23.306825630986427	28.55860599488756	28.685886519040988
4	13.802888467626884	15.534601027966152	35.57312881962572	35.089381684781245
5	15.151638663497147	36.366953421728155	33.571391680985805	14.910016233788895
6	34.3236996542009	34.09391093371338	17.07623706740934	14.506152344676385
7	29.942947320019552	30.928562500139506	20.434502139475498	18.693988040365443
8	27.24164130558221	33.814029006923455	19.693306431563578	19.251023255930754
9	27.189047189398718	15.736309405237586	18.785895910021477	38.28874749534222
10-11	26.143327102480395	24.69178343528746	27.29075920192442	21.874130260307727
12-13	26.814297762861507	22.089302768152812	27.01709054101095	24.07930892797473
14-15	23.969836497843193	23.526976292968715	24.270528194800438	28.232659014387657
16-17	24.404871118021738	26.512066801161595	24.96070565047514	24.12235643034153
18-19	24.384515392451217	26.033173489667078	26.113904445908304	23.468406671973398
20-21	24.937436632660077	25.21894213811084	25.976036664219055	23.867584565010024
22-23	24.853114762425122	24.896902994275017	25.74890365458704	24.501078588712826
24-25	24.712249043365077	25.115442947469536	25.62280167227445	24.54950633689094
26-27	24.36896190660222	25.327746087730546	25.731651764942242	24.571640240724992
28-29	24.57217495969021	25.539902854733814	25.469648451766503	24.41827373380947
30-31	24.81940441051942	25.29614719178776	25.332292815544598	24.55215558214822
32-33	24.56839494688995	25.382117139669745	25.22330941405892	24.826178499381385
34-35	24.83604733643618	25.409527688829957	25.329331669615833	24.425093305118022
36-37	24.94234233379709	25.210342029153704	25.378244612457067	24.469071024592143
38-39	24.757451992924327	25.293972327536025	25.36366333662864	24.58491234291101
40-41	24.92351431980158	25.104334795324696	25.434325537394464	24.537825347479263
42-43	24.809167723369857	25.176297801400203	25.257967709835107	24.756566765394833
44-45	24.645952894731177	25.225117181701457	25.397168182264195	24.73176174130317
46-47	24.811562477721726	25.184062243581412	25.271442010783034	24.732933267913833
48-49	24.64522283653137	25.219545739283994	25.272626187687266	24.862605236497373
50-51	24.67077610035077	25.30421672179242	25.466127365233117	24.55887981262369
52-53	24.96489439941779	25.0566966807611	25.355602783956254	24.62280613586486
54-55	24.469212485966978	24.968942484430702	25.630715341217496	24.931129688384818
56-57	24.46076353668888	25.143595088690095	25.581411928904775	24.814229445716247
58-59	24.643234666762478	25.00316309210463	25.468896074564253	24.88470616656864
60-61	24.61910492225788	25.027931070689412	25.69197121705088	24.660992790001828
62-63	24.60745184824216	25.084793196582243	25.61153637597729	24.696218579198305
64-65	24.4212749717973	24.93032150380859	25.569918236628702	25.07848528776541
66-67	24.43153217467713	24.988554948896013	25.55719401315143	25.02271886327543
68-69	24.536093137809907	24.982241027210854	25.608173181085608	24.873492653893624
70-71	24.416426721410662	24.781121147790206	25.625957667045856	25.176494463753272
72-73	24.39534464369834	24.956590172367278	25.553117106589262	25.094948077345116
74-75	24.36463289084958	25.073138070166976	25.558367527801135	25.003861511182308
76-77	24.34127139875556	24.751746760170125	25.657554496625007	25.24942734444931
78-79	24.31032638821415	24.86331230947639	25.679740695667615	25.146620606641847
80-81	24.42288859943525	24.969729475186	25.577279181764645	25.03010274361411
82-83	24.38206806143638	24.887643654215378	25.696298951769542	25.0339893325787
84-85	24.31222988411257	24.944618946898082	25.65538382128078	25.087767347708574
86-87	24.231528005633738	25.05385107179155	25.60967355986488	25.104947362709833
88-89	24.12525752395004	24.773559299093005	25.719186404692994	25.381996772263953
90-91	24.12529180245893	24.80543939080792	25.804384748831044	25.26488405790211
92-93	24.251032599219226	24.639490419853534	25.849692929413266	25.25978405151397
94-95	24.082276569372787	24.55872926557515	25.885268210720376	25.473725954331687
96-97	24.100392149843135	24.42243832121494	25.94785394383148	25.529315585110446
98-99	23.86676675092165	24.41556146092435	25.93333974707844	25.784332041075565
100-101	24.10321749522644	24.31515057947022	26.023117277657654	25.55851464764569
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	53.0
1	44.5
2	62.0
3	92.5
4	93.5
5	99.5
6	143.0
7	353.0
8	583.5
9	606.0
10	539.0
11	499.0
12	537.0
13	592.0
14	694.5
15	809.0
16	918.5
17	1064.5
18	1145.0
19	1256.0
20	1378.0
21	1459.5
22	1572.5
23	1687.0
24	1786.0
25	1985.0
26	2327.0
27	2734.0
28	3282.0
29	4150.0
30	5566.5
31	8118.0
32	12592.5
33	17605.5
34	23178.5
35	30991.0
36	41379.0
37	52968.0
38	65008.5
39	76586.5
40	87042.0
41	97083.5
42	107885.5
43	119256.0
44	129851.0
45	140303.0
46	149065.0
47	156475.0
48	163336.0
49	167937.0
50	168173.0
51	167625.0
52	167269.0
53	166239.0
54	165359.0
55	158354.0
56	146475.0
57	132225.0
58	120099.0
59	106287.5
60	87246.0
61	72540.0
62	60451.5
63	46747.5
64	34192.0
65	25234.5
66	18848.0
67	14347.5
68	10833.0
69	8013.5
70	6178.5
71	4648.0
72	3256.5
73	2382.0
74	1800.5
75	1330.5
76	946.5
77	654.0
78	441.5
79	300.0
80	224.5
81	186.0
82	137.5
83	101.0
84	95.5
85	83.0
86	70.0
87	68.0
88	60.0
89	55.0
90	42.5
91	32.5
92	24.0
93	16.0
94	20.0
95	19.5
96	16.5
97	14.5
98	12.0
99	10.5
100	8.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.058026906630243624
2	0.07850370925841613
3	0.07510020800414223
4	0.07571395413196212
5	0.06999495612273136
6	0.010656864583054357
7	0.013614005017095619
8	0.017882330360570273
9	0.006193256380727925
10-11	0.013879031754108751
12-13	0.023336301632787878
14-15	0.04106519546140318
16-17	0.028274168206611494
18-19	0.011633278877313264
20-21	0.02169034610818001
22-23	0.03876364748207861
24-25	0.03572281439424373
26-27	0.021941424069560872
28-29	0.011912254389958667
30-31	0.012023844595016828
32-33	0.009359628449253239
34-35	0.005621356579804851
36-37	6.974387816135051E-4
38-39	0.004198581465313301
40-41	0.00138092878759474
42-43	0.23890068025389002
44-45	0.20663716221644926
46-47	0.2128304185971772
48-49	0.22032091111170624
50-51	0.21121236062383386
52-53	0.21859126293330478
54-55	0.2151598641277663
56-57	0.2181588508887044
58-59	0.22704422096646043
60-61	0.23237265325798764
62-63	0.2163036637296125
64-65	0.21645710026156742
66-67	0.2139881669746556
68-69	0.20864578590749616
70-71	0.19989990358606283
72-73	0.2117145165465956
74-75	0.21136579715578885
76-77	0.20504700179437052
78-79	0.22461713400644545
80-81	0.23595748859548105
82-83	0.23495317674995758
84-85	0.22835540587589384
86-87	0.2494599034075185
88-89	0.24167648660471178
90-91	0.24892984993349224
92-93	0.24375485417392004
94-95	0.2468375335886517
96-97	0.25456515528892937
98-99	0.2480092307417624
100-101	0.25450936018640025
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3584544.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.37631849398355
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.8550300474157	40.70340954734984
2	13.035186085894315	14.176108604698642
3	4.719168228115532	7.698329837960944
4	2.26569173341987	4.927999012224982
5	1.3247926322270134	3.6018673054229446
6	0.8224285881936675	2.6832383310105654
7	0.5753819880343778	2.190100796594113
8	0.4144928474827238	1.8030876070558988
9	0.29874370691203606	1.4620124659609934
>10	1.5903374876214995	15.245325080733332
>50	0.07669451396919522	2.7966090556562575
>100	0.020303057027301515	1.7615803186099823
>500	0.0011317600385670479	0.43663299834116737
>1k	6.173236481249949E-4	0.5136990383803297
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7897551264540205E-5
2	0.0	0.0	0.0	0.0	2.7897551264540205E-5
3	0.0	2.7897551264540205E-5	0.0	2.7897551264540205E-5	2.7897551264540205E-5
4	0.0	2.7897551264540205E-5	0.0	2.7897551264540205E-5	5.579510252908041E-5
5	0.0	2.7897551264540205E-5	0.0	2.7897551264540205E-5	8.369265379362061E-5
6	0.0	2.7897551264540205E-5	0.0	2.7897551264540205E-5	8.369265379362061E-5
7	0.0	2.7897551264540205E-5	0.0	2.7897551264540205E-5	8.369265379362061E-5
8	0.0	2.7897551264540205E-5	0.0	2.7897551264540205E-5	8.369265379362061E-5
9	0.0	2.7897551264540205E-5	0.0	2.7897551264540205E-5	1.1159020505816082E-4
10-11	0.0	2.7897551264540205E-5	0.0	2.7897551264540205E-5	1.3948775632270102E-4
12-13	0.0	5.579510252908041E-5	0.0	2.7897551264540205E-5	2.9292428827767215E-4
14-15	0.0	1.2553898069043092E-4	0.0	2.7897551264540205E-5	4.184632689681031E-4
16-17	0.0	1.6738530758724122E-4	0.0	2.7897551264540205E-5	4.463608202326433E-4
18-19	0.0	1.6738530758724122E-4	0.0	2.7897551264540205E-5	4.7425837149718346E-4
20-21	0.0	1.6738530758724122E-4	0.0	2.7897551264540205E-5	5.579510252908041E-4
22-23	0.0	1.6738530758724122E-4	0.0	5.579510252908041E-5	6.276949034521546E-4
24-25	0.0	1.8133408321951132E-4	0.0	1.8133408321951132E-4	7.113875572457752E-4
26-27	0.0	1.9528285885178143E-4	0.0	2.9292428827767215E-4	7.532338841425855E-4
28-29	0.0	1.9528285885178143E-4	0.0	0.0013251336850656596	7.532338841425855E-4
30-31	0.0	1.9528285885178143E-4	0.0	0.006681463527857379	7.671826597748556E-4
32-33	0.0	1.9528285885178143E-4	0.0	0.0219274752939286	8.369265379362061E-4
34-35	2.7897551264540205E-5	2.2318041011632164E-4	0.0	0.043422538543256825	8.648240892007463E-4
36-37	2.7897551264540205E-5	2.5107796138086185E-4	0.0	0.0788942749761197	8.648240892007463E-4
38-39	2.7897551264540205E-5	2.7897551264540203E-4	0.0	0.13706066936268602	9.345679673620968E-4
40-41	2.7897551264540205E-5	2.7897551264540203E-4	0.0	0.20496330914057687	9.485167429943669E-4
42-43	2.7897551264540205E-5	3.3477061517448245E-4	0.0	0.27813858610746583	9.764142942589072E-4
44-45	2.7897551264540205E-5	3.3477061517448245E-4	0.0	0.3696286054795254	0.0010182606211557174
46-47	2.7897551264540205E-5	3.9056571770356287E-4	0.0	0.47664361213030165	0.0011019532749493382
48-49	2.7897551264540205E-5	4.463608202326433E-4	0.0	0.5934367104992992	0.0011716971531106884
50-51	2.7897551264540205E-5	4.603095958649134E-4	0.0	0.7157256264674112	0.001227492255639769
52-53	2.7897551264540205E-5	4.7425837149718346E-4	0.0	0.8422689190033655	0.0013530312363302
54-55	2.7897551264540205E-5	5.440022496585339E-4	0.0	0.9930970299151021	0.0014785702170206309
56-57	2.7897551264540205E-5	5.579510252908041E-4	0.0	1.1652388699929475	0.0015622628708142515
58-59	2.7897551264540205E-5	5.579510252908041E-4	0.0	1.345345460956819	0.001645955524607872
60-61	2.7897551264540205E-5	5.579510252908041E-4	0.0	1.5433762286081576	0.0016738530758724122
62-63	2.7897551264540205E-5	5.579510252908041E-4	0.0	1.7506410857280592	0.0017575457296660328
64-65	2.7897551264540205E-5	5.579510252908041E-4	0.0	1.97264979869127	0.0018133408321951133
66-67	2.7897551264540205E-5	5.858485765553443E-4	0.0	2.2308276868689574	0.0018970334859887339
68-69	2.7897551264540205E-5	5.858485765553443E-4	0.0	2.5164149191640552	0.001924931037253274
70-71	2.7897551264540205E-5	5.858485765553443E-4	0.0	2.8152255907585455	0.0019807261397823545
72-73	2.7897551264540205E-5	5.858485765553443E-4	0.0	3.1329229045591296	0.0022178553255309463
74-75	2.7897551264540205E-5	5.858485765553443E-4	0.0	3.4712923038467376	0.0022736504280600266
76-77	2.7897551264540205E-5	6.137461278198845E-4	0.0	3.8475047314246944	0.002315496754956837
78-79	5.579510252908041E-5	6.137461278198845E-4	0.0	4.268492728782238	0.002329445530589107
80-81	5.579510252908041E-5	6.137461278198845E-4	0.0	4.702285702170206	0.0023712918574859175
82-83	5.579510252908041E-5	6.137461278198845E-4	0.0	5.165357155610309	0.0025665747163376986
84-85	5.579510252908041E-5	6.137461278198845E-4	0.0	5.655028924181151	0.002594472267602239
86-87	5.579510252908041E-5	6.137461278198845E-4	0.0	6.178624115089674	0.002664216145763589
88-89	5.579510252908041E-5	6.137461278198845E-4	0.0	6.744498044939608	0.0027758063508217506
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4745	0.0	46.01376	1
GGTATCA	2540	0.0	42.04506	1
TCAACGC	5775	0.0	37.068314	4
CAACGCA	5885	0.0	36.45203	5
ATCAACG	6145	0.0	34.913128	3
TATCAAC	6365	0.0	34.005154	2
AACGCAG	6470	0.0	33.357105	6
ACGCAGA	7465	0.0	28.660816	7
GTACATG	10715	0.0	28.43865	1
CGCAGAG	7740	0.0	27.092773	8
TACATGG	11355	0.0	26.376703	2
ACATGGG	11735	0.0	24.834616	3
CATGGGG	9950	0.0	23.279573	4
GCAGAGT	9255	0.0	21.171806	9
AGAGTAC	7600	0.0	19.25865	10-11
GAGTACA	8170	0.0	18.939201	1
GTATATA	725	0.0	18.331	1
AGTACAT	8065	0.0	18.009237	2
ATGGGGG	5770	0.0	17.931244	5
GTATTAA	1675	0.0	17.0021	1
>>END_MODULE