FastQCFastQC Report
Mon 15 Aug 2016
SRR2921684_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921684_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3584544
Sequences flagged as poor quality0
Sequence length101
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA45500.047.8554461
GGTATCA24900.044.6781
TCAACGC60500.035.069194
TATCAAC63100.034.0002942
CAACGCA63100.033.65335
ATCAACG63300.033.5179523
AACGCAG67600.031.2659686
GTACATG102450.026.358071
ACGCAGA82450.025.0579197
TACATGG110550.023.9149822
CGCAGAG86450.023.513938
ACATGGG113000.022.72443
GTATACG2354.609319E-922.253691
CATGGGG97700.020.2102814
GCAGAGT100000.018.5229859
AGAGTAC81900.018.0352810-11
TAAGGTA9700.017.6158814
ATGGGGG55350.017.509035
GTATAGG7600.016.8899141
GTGTAAG18250.016.411751
Produced by FastQC (version 0.11.5)