##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921684_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3584544
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.386409540516173	33.0	31.0	34.0	29.0	34.0
2	31.52252336698894	34.0	31.0	34.0	28.0	34.0
3	31.59487315541391	34.0	31.0	34.0	28.0	34.0
4	35.07761266147103	37.0	35.0	37.0	32.0	37.0
5	34.81984486729693	37.0	35.0	37.0	32.0	37.0
6	34.791955685297765	37.0	35.0	37.0	32.0	37.0
7	34.6816515573529	37.0	35.0	37.0	32.0	37.0
8	34.62412959640054	37.0	35.0	37.0	32.0	37.0
9	36.21311246284046	39.0	37.0	39.0	32.0	39.0
10-11	36.10948282403564	39.0	37.0	39.0	32.0	39.0
12-13	36.02796241307123	39.0	37.0	39.0	31.0	39.0
14-15	37.268454230161495	40.0	38.0	41.0	31.0	41.0
16-17	37.235656892480606	40.0	38.0	41.0	31.0	41.0
18-19	37.1873588383906	40.0	38.0	41.0	31.0	41.0
20-21	37.126126921583335	40.0	38.0	41.0	31.0	41.0
22-23	37.037399736200754	40.0	37.5	41.0	31.0	41.0
24-25	36.9493645495773	40.0	37.0	41.0	30.5	41.0
26-27	36.82269767088924	40.0	37.0	41.0	30.0	41.0
28-29	36.69856863243972	40.0	37.0	41.0	30.0	41.0
30-31	36.546329742360534	40.0	37.0	41.0	30.0	41.0
32-33	36.50248636925645	40.0	36.5	41.0	30.0	41.0
34-35	36.638272260014105	40.0	37.0	41.0	30.0	41.0
36-37	36.637199599167985	40.0	37.0	41.0	30.0	41.0
38-39	36.53321482453556	40.0	37.0	41.0	30.0	41.0
40-41	36.44907511248293	40.0	37.0	41.0	30.0	41.0
42-43	36.32961333435996	40.0	36.5	41.0	29.5	41.0
44-45	36.19975651017256	40.0	36.0	41.0	29.0	41.0
46-47	36.042555761625465	40.0	36.0	41.0	28.0	41.0
48-49	35.89546215641376	40.0	35.5	41.0	28.0	41.0
50-51	35.73192922168064	39.5	35.0	41.0	28.0	41.0
52-53	35.55217246600962	39.0	35.0	41.0	27.0	41.0
54-55	35.3271863868877	39.0	35.0	41.0	27.0	41.0
56-57	35.08800924190078	39.0	35.0	41.0	26.0	41.0
58-59	34.836111232000505	38.0	35.0	40.0	26.0	41.0
60-61	34.542505964496456	38.0	34.0	40.0	26.0	41.0
62-63	34.22701729982949	37.0	34.0	40.0	26.0	41.0
64-65	33.87784638715552	37.0	34.0	39.5	25.0	41.0
66-67	33.514883064624115	36.0	34.0	39.0	24.0	41.0
68-69	33.14688995308748	36.0	33.5	39.0	23.0	40.5
70-71	32.74494552166189	35.0	33.0	38.5	22.5	40.0
72-73	32.38251769262701	35.0	33.0	37.0	22.0	39.5
74-75	31.98193870684807	35.0	33.0	37.0	20.5	39.0
76-77	29.29634843371988	33.0	29.0	34.5	15.0	36.0
78-79	30.869747449047914	34.5	31.5	35.0	18.0	37.0
80-81	31.063768501656	35.0	32.0	35.0	19.0	37.0
82-83	30.908134619075675	35.0	32.0	35.0	18.0	36.5
84-85	30.727313013872894	35.0	32.0	35.0	17.5	36.0
86-87	30.536899672594338	35.0	32.0	35.0	15.0	36.0
88-89	30.282784923270576	35.0	32.0	35.0	7.0	35.5
90-91	30.09988188176795	34.0	31.0	35.0	4.5	35.0
92-93	29.948463598159208	34.0	31.0	35.0	2.0	35.0
94-95	29.8527786240035	34.0	31.0	35.0	2.0	35.0
96-97	29.65473195474794	34.0	31.0	35.0	2.0	35.0
98-99	29.462139256764598	34.0	31.0	35.0	2.0	35.0
100-101	28.351070735915087	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	65377.0
3	22248.0
4	12273.0
5	8723.0
6	7738.0
7	7929.0
8	8292.0
9	8835.0
10	9689.0
11	10560.0
12	11008.0
13	11687.0
14	12407.0
15	13150.0
16	14061.0
17	14990.0
18	14565.0
19	13302.0
20	12604.0
21	12814.0
22	13595.0
23	15158.0
24	18431.0
25	21919.0
26	26803.0
27	32753.0
28	38934.0
29	47351.0
30	57672.0
31	71482.0
32	91335.0
33	120288.0
34	172560.0
35	259596.0
36	461450.0
37	886650.0
38	870136.0
39	86178.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.166048707203466	19.04280184348281	13.67714570914062	26.114003740173104
2	15.22318490606463	20.683161767803334	37.441495266197414	26.652158059934628
3	18.058714929783537	24.591121250869456	28.44282198390628	28.907341835440725
4	13.43529097321401	16.856071438236597	34.65704172559265	35.051595862956745
5	14.866781263429306	36.85794652275099	33.6081516249114	14.667120588908297
6	34.117784580601985	34.92036036679455	16.88689980831138	14.074955244292095
7	29.617407402447842	31.283644446825036	20.575029906174954	18.523918244552164
8	27.167890811216154	34.361943946008196	19.496343188980244	18.973822053795406
9	26.955590446093	15.79157627860057	18.77033731487185	38.482495960434576
10-11	26.02652072272507	25.0253471813725	27.30946141722877	21.63867067867366
12-13	26.672580041536833	22.29607818822526	27.135527828687138	23.895813941550767
14-15	23.819712195693537	23.780556191028854	24.30756504611676	28.09216656716085
16-17	24.325367466545256	26.839941147325852	24.94905907139095	23.885632314737943
18-19	24.28070419179585	26.305587591833458	26.123720106525973	23.289988109844725
20-21	24.860675772544525	25.401819957375217	25.978519665524214	23.758984604556044
22-23	24.69799505878572	25.119987367988788	25.79037947365132	24.391638099574173
24-25	24.586817121006842	25.333932390678193	25.672192417367956	24.40705807094701
26-27	24.261003381020256	25.574799946769645	25.75413155988094	24.41006511232916
28-29	24.471240977408367	25.721751532222427	25.549446257270525	24.25756123309868
30-31	24.701788231243327	25.527396168061955	25.34201176398534	24.428803836709374
32-33	24.557336816865146	25.540707267817712	25.24162258775001	24.660333327567127
34-35	24.72219195800874	25.6033990532548	25.368421654693428	24.305987334043028
36-37	24.860440127458105	25.379356368099653	25.465967952458346	24.2942355519839
38-39	24.75384595641733	25.471789438210273	25.35312720390655	24.42123740146585
40-41	24.828576418453764	25.327712116629968	25.439125587686185	24.404585877230083
42-43	24.75024806745928	25.330683374409368	25.222793385369958	24.696275172761393
44-45	24.60123213734943	25.344844590633013	25.39704505212304	24.656878219894512
46-47	24.7226376809896	25.35240993518843	25.28302576365134	24.641926620170622
48-49	24.57899062095492	25.396785096646724	25.278711773168645	24.74551250922971
50-51	24.589624717441325	25.482736807011992	25.3573334133088	24.570305062237885
52-53	24.888114123872633	25.251642090180493	25.268841352774313	24.591402433172565
54-55	24.401421108304337	25.161788540881	25.515628829544145	24.92116152127052
56-57	24.447028589946097	25.32156313337004	25.445424488445905	24.785983788237957
58-59	24.58085999750835	25.201944042307208	25.359966885934348	24.85722907425009
60-61	24.535289174482294	25.26352631719914	25.57979695737377	24.621387550944796
62-63	24.53006587797632	25.305832136029693	25.449187679822266	24.714914306171725
64-65	24.322985436696758	25.112746218511788	25.454913094916144	25.109355249875314
66-67	24.347126519242398	25.253071563768508	25.348218962679802	25.051582954309286
68-69	24.438169239069786	25.197357752113135	25.384576522724302	24.979896486092777
70-71	24.300960118993114	25.033849275388214	25.431665501600197	25.233525104018472
72-73	24.32122887995143	25.22348491772448	25.288090250999396	25.167195951324693
74-75	24.290752211679397	25.330201414386504	25.27172559478671	25.10732077914739
76-77	24.296401498866317	25.002408303074002	25.40708710814742	25.29410308991227
78-79	24.194150738853672	25.138657658997165	25.498154698207472	25.169036903941695
80-81	24.341957506562174	25.22268819883406	25.300017517742823	25.135336776860946
82-83	24.305522030160855	25.17840703509595	25.404651126448613	25.11141980829458
84-85	24.185650091287354	25.226529687719374	25.360592724421604	25.227227496571665
86-87	24.12705365271086	25.327915830214348	25.296872526760193	25.248157990314603
88-89	23.981193788174934	25.126810841468295	25.39298874584823	25.499006624508542
90-91	23.92726026247307	25.16007219408456	25.455690181044854	25.456977362397517
92-93	24.01573940493784	25.0133248181035	25.52569006787109	25.445245709087576
94-95	23.89545599373248	24.939540103018977	25.536396746381474	25.628607156867066
96-97	23.82585896272966	24.80209977502809	25.6150315722392	25.757009690003052
98-99	23.597044228469006	24.81273255428737	25.608740379619267	25.981482837624366
100-101	23.58889764873465	24.84251805951004	25.6320340306059	25.936550261149417
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	43.0
1	38.0
2	44.0
3	65.0
4	63.5
5	73.0
6	120.5
7	320.5
8	506.5
9	509.5
10	427.0
11	344.5
12	323.5
13	338.0
14	367.5
15	405.5
16	496.5
17	581.5
18	626.0
19	702.5
20	767.5
21	849.0
22	1031.0
23	1174.0
24	1353.5
25	1683.5
26	2120.5
27	2645.0
28	3416.0
29	4510.5
30	6312.5
31	9355.5
32	14182.5
33	19438.0
34	25248.5
35	32884.0
36	43224.5
37	54828.0
38	66096.5
39	77438.0
40	88326.5
41	98533.0
42	109464.0
43	120538.5
44	130964.0
45	141088.5
46	150135.5
47	157541.0
48	164414.5
49	169113.0
50	168990.0
51	168862.0
52	168613.0
53	167084.5
54	164856.0
55	157514.5
56	145423.0
57	131257.0
58	119222.5
59	104771.0
60	85693.0
61	70904.0
62	58752.0
63	44856.5
64	32228.0
65	23683.5
66	17644.0
67	13359.0
68	9983.0
69	7334.0
70	5607.5
71	4020.5
72	2668.0
73	1992.5
74	1510.5
75	1036.0
76	667.0
77	388.0
78	224.0
79	125.5
80	77.0
81	44.0
82	28.5
83	16.5
84	10.0
85	9.5
86	9.0
87	6.5
88	3.0
89	3.5
90	3.5
91	3.0
92	3.5
93	2.0
94	1.0
95	0.5
96	0.5
97	0.0
98	0.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.17017506271369526
2	1.3948775632270102E-4
3	0.011521688672255104
4	0.00683490005981235
5	0.026893239419016758
6	0.0023712918574859175
7	0.0
8	0.0
9	0.0
10-11	0.05017374594927555
12-13	4.0451449333583293E-4
14-15	0.004575198407384593
16-17	0.0
18-19	0.0018412383834596536
20-21	2.650267370131319E-4
22-23	0.0
24-25	6.555924547166948E-4
26-27	0.0034313988055384454
28-29	0.005802690663024362
30-31	0.01784048403367346
32-33	0.012330717658926769
34-35	0.010308145192247606
36-37	0.004198581465313301
38-39	0.0
40-41	0.00412883758715195
42-43	0.009415423551782319
44-45	0.007936853334761688
46-47	0.004268325343474651
48-49	0.009220140692930538
50-51	0.0031105769659962325
52-53	0.002454984511279538
54-55	0.004421761875429622
56-57	0.00821582884740709
58-59	0.015859757893891106
60-61	0.007588133943954935
62-63	0.022234348357838545
64-65	0.04176263424301668
66-67	0.0615838444164725
68-69	0.05124780167296035
70-71	0.06940910754617603
72-73	0.03483009275377844
74-75	0.028218373104082414
76-77	0.23362804306489193
78-79	0.08791913281019845
80-81	0.06866982243766571
82-83	0.028594990046153707
84-85	0.053033244953890925
86-87	0.06837689814938804
88-89	0.29623014810252013
90-91	0.302521045912674
92-93	0.05421889088263388
94-95	0.009764142942589071
96-97	0.015092575234116251
98-99	8.787728648330164E-4
100-101	1.6738530758724122E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3584544.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.10584512014296
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.68317218887722	39.13007130223338
2	13.478387399949998	14.315623074620618
3	5.039232798663026	8.028381495904295
4	2.4246903673793607	5.150609244574034
5	1.3994008649063832	3.7158182796356236
6	0.8548148266987892	2.723739827584064
7	0.5846446647235728	2.173363431058957
8	0.41122797988593546	1.7470887527113403
9	0.3275196284220173	1.5653885994707781
>10	1.6939455325238113	15.84137381168014
>50	0.08017654157645471	2.8769152491453123
>100	0.02104878825426374	1.8085092181770421
>500	0.001211624768464835	0.4732667340951843
>1k	5.267933707720062E-4	0.4498509791092621
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.7897551264540205E-5
2	0.0	0.0	0.0	0.0	2.7897551264540205E-5
3	0.0	2.7897551264540205E-5	0.0	0.0	2.7897551264540205E-5
4	0.0	2.7897551264540205E-5	0.0	0.0	2.7897551264540205E-5
5	0.0	2.7897551264540205E-5	0.0	0.0	2.7897551264540205E-5
6	0.0	2.7897551264540205E-5	0.0	0.0	5.579510252908041E-5
7	0.0	2.7897551264540205E-5	0.0	0.0	5.579510252908041E-5
8	0.0	2.7897551264540205E-5	0.0	0.0	5.579510252908041E-5
9	0.0	2.7897551264540205E-5	0.0	0.0	8.369265379362061E-5
10-11	0.0	2.7897551264540205E-5	0.0	0.0	8.369265379362061E-5
12-13	0.0	4.1846326896810306E-5	0.0	1.3948775632270103E-5	3.9056571770356287E-4
14-15	0.0	6.974387816135051E-5	0.0	2.7897551264540205E-5	4.603095958649134E-4
16-17	0.0	8.369265379362061E-5	0.0	2.7897551264540205E-5	5.161046983939938E-4
18-19	0.0	9.764142942589072E-5	0.0	4.1846326896810306E-5	5.997973521876144E-4
20-21	0.0	1.1159020505816082E-4	0.0	5.579510252908041E-5	7.253363328780453E-4
22-23	0.0	1.1159020505816082E-4	0.0	8.369265379362061E-5	8.22977762303936E-4
24-25	0.0	1.3948775632270102E-4	0.0	2.0923163448405155E-4	8.648240892007463E-4
26-27	0.0	1.6738530758724122E-4	0.0	3.0687306390994227E-4	9.345679673620968E-4
28-29	0.0	1.6738530758724122E-4	0.0	0.00136698001196247	9.485167429943669E-4
30-31	0.0	1.6738530758724122E-4	0.0	0.006904643937973701	9.624655186266371E-4
32-33	0.0	1.6738530758724122E-4	0.0	0.02233198978726443	0.0010322093967879875
34-35	0.0	1.9528285885178143E-4	0.0	0.044315260183722116	0.0011159020505816081
36-37	0.0	2.5107796138086185E-4	0.0	0.08041469152003713	0.001129850826213878
38-39	0.0	3.4871939080675257E-4	0.0	0.13975278305971414	0.0011577483774784184
40-41	0.0	4.0451449333583293E-4	0.0	0.20826916896542488	0.0011716971531106886
42-43	0.0	4.463608202326433E-4	0.0	0.28233716757277916	0.0011716971531106886
44-45	0.0	4.463608202326433E-4	0.0	0.37439908674576183	0.0011995947043752287
46-47	0.0	4.463608202326433E-4	0.0	0.48264158565217785	0.001241441031272039
48-49	0.0	4.7425837149718346E-4	0.0	0.6001042252515243	0.0012553898069043092
50-51	0.0	5.021559227617237E-4	0.0	0.7231324263281467	0.0013390824606979298
52-53	0.0	5.300534740262638E-4	0.0	0.8506521331583599	0.0014506726657560905
54-55	0.0	5.300534740262638E-4	0.0	1.0007967540641152	0.0015901604220787916
56-57	0.0	5.300534740262638E-4	0.0	1.1733570574109287	0.0016320067489756019
58-59	0.0	5.300534740262638E-4	0.0	1.3550259112456144	0.001645955524607872
60-61	0.0	5.300534740262638E-4	0.0	1.553614629922244	0.001645955524607872
62-63	0.0	5.300534740262638E-4	0.0	1.761535079496862	0.0017854432809305731
64-65	0.0	5.300534740262638E-4	0.0	1.9840180508315701	0.0017854432809305731
66-67	0.0	5.579510252908041E-4	0.0	2.243144455752252	0.0018412383834596536
68-69	0.0	5.579510252908041E-4	0.0	2.5293872805020667	0.0018691359347241937
70-71	0.0	5.579510252908041E-4	0.0	2.8304437049733524	0.001883084710356464
72-73	0.0	5.579510252908041E-4	0.0	3.1510144665541837	0.0020225724666791648
74-75	0.0	5.579510252908041E-4	0.0	3.493108188935608	0.002064418793575975
76-77	0.0	5.858485765553443E-4	0.0	3.8729194006266905	0.002064418793575975
78-79	0.0	5.858485765553443E-4	0.0	4.298496545167252	0.002078367569208245
80-81	0.0	5.858485765553443E-4	0.0	4.736669434103752	0.0021341626717373257
82-83	0.0	5.858485765553443E-4	0.0	5.2049995759572205	0.0022875992036922965
84-85	0.0	5.858485765553443E-4	0.0	5.700641420498675	0.0022875992036922965
86-87	0.0	5.858485765553443E-4	0.0	6.229760326557576	0.0023712918574859175
88-89	0.0	5.858485765553443E-4	0.0	6.802971312390083	0.002427086960014998
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	4550	0.0	47.855446	1
GGTATCA	2490	0.0	44.678	1
TCAACGC	6050	0.0	35.06919	4
TATCAAC	6310	0.0	34.000294	2
CAACGCA	6310	0.0	33.6533	5
ATCAACG	6330	0.0	33.517952	3
AACGCAG	6760	0.0	31.265968	6
GTACATG	10245	0.0	26.35807	1
ACGCAGA	8245	0.0	25.057919	7
TACATGG	11055	0.0	23.914982	2
CGCAGAG	8645	0.0	23.51393	8
ACATGGG	11300	0.0	22.7244	3
GTATACG	235	4.609319E-9	22.25369	1
CATGGGG	9770	0.0	20.210281	4
GCAGAGT	10000	0.0	18.522985	9
AGAGTAC	8190	0.0	18.03528	10-11
TAAGGTA	970	0.0	17.615881	4
ATGGGGG	5535	0.0	17.50903	5
GTATAGG	760	0.0	16.889914	1
GTGTAAG	1825	0.0	16.41175	1
>>END_MODULE