FastQCFastQC Report
Mon 15 Aug 2016
SRR2921683_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921683_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103926
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA37550.044.109051
TCAACGC42150.038.623424
CAACGCA43700.036.9234855
GGTATCA21250.036.403311
ATCAACG45100.035.7796063
AACGCAG46250.034.8725246
TATCAAC48650.033.461432
ACGCAGA56600.027.912137
CGCAGAG57900.026.6333628
GTACATG79900.024.7092061
TACATGG83950.022.6702792
ACATGGG84050.021.8527663
GCAGAGT74200.020.3353399
CATGGGG69900.019.216024
AGAGTAC59950.018.87675710-11
GAGTACA58500.018.4154431
GTATAGC9700.018.1026521
TACACCG5100.017.6802585
GTATAGG9300.016.8400421
AGTACAT58400.016.8225062
Produced by FastQC (version 0.11.5)