##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921683_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3103926
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.242085346106833	31.0	31.0	34.0	26.0	34.0
2	30.3082567045735	31.0	31.0	34.0	26.0	34.0
3	30.374988321242196	33.0	31.0	34.0	26.0	34.0
4	33.816806843977595	37.0	35.0	37.0	29.0	37.0
5	33.81317885800112	37.0	35.0	37.0	29.0	37.0
6	33.846026612747856	37.0	35.0	37.0	29.0	37.0
7	33.76166764284973	37.0	35.0	37.0	29.0	37.0
8	33.72815234641548	37.0	35.0	37.0	29.0	37.0
9	35.269965843257864	39.0	35.0	39.0	29.0	39.0
10-11	35.16823951344201	39.0	35.0	39.0	28.0	39.0
12-13	35.06147375936153	39.0	35.0	39.0	27.0	39.0
14-15	36.190289330351305	40.0	36.0	41.0	27.0	41.0
16-17	36.131441922262326	40.0	36.0	41.0	27.0	41.0
18-19	36.09119676177847	40.0	36.5	41.0	27.0	41.0
20-21	36.018900740546	40.0	36.0	41.0	27.0	41.0
22-23	35.91686560826514	40.0	36.0	41.0	27.0	41.0
24-25	35.814447734900895	40.0	36.0	41.0	27.0	41.0
26-27	35.670992961816744	40.0	36.0	41.0	26.5	41.0
28-29	35.53032208242078	40.0	36.0	41.0	25.5	41.0
30-31	35.38988590578512	40.0	36.0	41.0	25.0	41.0
32-33	35.22512827303228	39.0	35.0	41.0	24.5	41.0
34-35	35.0576569802244	39.0	35.0	41.0	24.0	41.0
36-37	35.03213333694167	39.0	35.0	41.0	24.0	41.0
38-39	35.214847100091944	39.5	35.0	41.0	24.5	41.0
40-41	35.27911941199629	40.0	35.0	41.0	24.0	41.0
42-43	35.12907121497098	40.0	35.0	41.0	23.5	41.0
44-45	35.01631417759315	40.0	35.0	41.0	23.0	41.0
46-47	34.867035006633536	40.0	35.0	41.0	22.5	41.0
48-49	34.71136683026593	39.0	35.0	41.0	21.5	41.0
50-51	33.04010469321756	37.0	32.5	39.5	17.0	40.0
52-53	33.15767289555228	37.5	32.5	39.5	16.5	40.0
54-55	34.0718570610253	39.0	34.0	40.5	18.0	41.0
56-57	34.09705627647051	39.0	34.0	41.0	18.0	41.0
58-59	33.91718520351323	38.5	34.0	41.0	16.0	41.0
60-61	33.66554067332791	38.0	34.0	40.0	13.5	41.0
62-63	33.380889396203386	37.5	33.5	40.0	12.5	41.0
64-65	33.09494040772879	37.0	33.0	40.0	9.5	41.0
66-67	32.757685428067546	36.5	33.0	39.5	7.5	41.0
68-69	32.40380183032714	36.0	33.0	39.0	6.0	41.0
70-71	31.996624919537386	35.0	32.0	39.0	2.0	40.5
72-73	31.63800361219952	35.0	32.0	37.5	2.0	40.0
74-75	31.239756521257274	35.0	32.0	37.0	2.0	39.0
76-77	30.864673481262116	35.0	31.5	37.0	2.0	39.0
78-79	30.523598178564825	35.0	31.0	36.0	2.0	38.5
80-81	30.214767523452558	35.0	31.0	36.0	2.0	37.0
82-83	29.909870757228106	35.0	31.0	35.0	2.0	37.0
84-85	29.65495134226782	35.0	31.0	35.0	2.0	36.5
86-87	29.432393684643255	35.0	31.0	35.0	2.0	36.0
88-89	29.22214092732881	35.0	30.5	35.0	2.0	36.0
90-91	29.01637990080949	34.0	30.0	35.0	2.0	35.5
92-93	28.839211695124174	34.0	30.0	35.0	2.0	35.0
94-95	28.62779557244599	34.0	30.0	35.0	2.0	35.0
96-97	28.413758575429956	34.0	30.0	35.0	2.0	35.0
98-99	28.165114761112218	34.0	29.5	35.0	2.0	35.0
100-101	27.11915892969098	33.0	27.0	35.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	114823.0
3	19388.0
4	12911.0
5	9965.0
6	8840.0
7	9180.0
8	9532.0
9	10267.0
10	11302.0
11	12064.0
12	11820.0
13	12020.0
14	12500.0
15	13131.0
16	13189.0
17	13445.0
18	13281.0
19	12657.0
20	12778.0
21	13220.0
22	14774.0
23	16783.0
24	19683.0
25	23415.0
26	27478.0
27	32654.0
28	38667.0
29	45939.0
30	55136.0
31	66767.0
32	83482.0
33	108220.0
34	148664.0
35	215630.0
36	360719.0
37	658354.0
38	719140.0
39	122090.0
40	18.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.182048037195074	17.71842985920977	13.223154348354438	24.87636775524071
2	15.591594759491942	19.00577234818247	40.13738988239937	25.26524300992622
3	19.498570512977576	23.402030812957072	28.480771884305046	28.61862678976031
4	13.111176090736201	15.665702826108612	36.906829426657254	34.316291656497924
5	14.636594048017413	36.75181933475566	34.2882785647001	14.323308052526823
6	33.85653236774468	35.036291737431284	17.294888933783003	13.812286961041037
7	29.59235523895356	30.891947861631554	20.79899185148691	18.716705047927977
8	26.552347919784186	34.93578713603151	19.5910779950429	18.9207869491414
9	26.42543856116304	16.11548559813051	19.11249339101449	38.346582449691965
10-11	25.72618687047694	25.16756671413934	27.752824194307145	21.353422221076578
12-13	26.43355958315724	22.32711480641212	27.558917087901015	23.680408522529625
14-15	23.442239260214926	23.931194341515674	24.759559215261095	27.8670071830083
16-17	23.940843155506496	26.9817786207231	25.374133075830756	23.703245147939644
18-19	23.87654206426535	26.46537991617563	26.474353273841743	23.183724745717278
20-21	24.478853074776122	25.694710847902257	26.407172826165237	23.41926325115638
22-23	24.16303814129261	25.529977412661186	26.182120953853794	24.124863492192404
24-25	24.055712038982634	25.64871153623039	26.203350392533487	24.092226032253492
26-27	23.997594171230496	25.845226197367865	26.0847134651813	24.07246616622034
28-29	24.1066911889325	25.920809363656222	25.96437204332811	24.00812740408316
30-31	24.301966055908448	25.75385668265039	25.901299598462934	24.04287766297823
32-33	24.04332718526823	25.777748493605912	25.873344488884424	24.305579832241435
34-35	24.326482113089273	25.8732905510639	25.87934768530481	23.92087965054202
36-37	24.429807336366714	25.605245260247813	25.86480522773395	24.100142175651527
38-39	24.197985184650598	25.805284991855164	25.858864336969294	24.13786548652494
40-41	24.520623479836587	25.594755503678197	25.89553824436761	23.989082772117605
42-43	24.250059332664932	25.582033261811237	25.840755970803485	24.327151434720346
44-45	24.259941211067503	25.683616636247837	25.95932291367401	24.09711923901065
46-47	24.387774306713457	25.65461234933448	25.712623637162473	24.24498970678958
48-49	24.2155605069395	25.719281066799937	25.7567150790626	24.30844334719796
50-51	24.2898244283126	25.77208365783092	25.786061601410708	24.152030312445778
52-53	24.435725271475594	25.6921675303695	25.744080095147208	24.128027103007703
54-55	24.111990049116827	25.554890707026328	26.035050520327818	24.298068723529028
56-57	24.14520322533938	25.723506872966222	25.941809526253863	24.18948037544053
58-59	24.277243424947205	25.587306056182303	25.909284555541333	24.22616596332916
60-61	24.226953913153817	25.592169010610142	26.06404120458579	24.116835871650245
62-63	24.17376336088074	25.61586198725517	26.028475878987685	24.181898772876405
64-65	24.05865781065959	25.53157591696815	25.967548312646883	24.442217959725376
66-67	24.05303938246299	25.610827913517316	25.9369449944563	24.399187709563392
68-69	24.17690239233523	25.496770299017697	25.961856924730697	24.364470383916377
70-71	24.042693258847855	25.353096710563584	25.971888477716732	24.63232155287183
72-73	24.046015549641474	25.455871661869544	25.90115479524756	24.596957993241418
74-75	24.090344015647553	25.552750281899332	25.903280362406495	24.45362534004662
76-77	24.082453680853764	25.322983986940866	26.01419215348159	24.58037017872378
78-79	24.0168801191591	25.348041072890354	26.01723751897375	24.6178412889768
80-81	24.089823889794054	25.468641327616904	25.894968030744213	24.54656675184483
82-83	24.044220376683967	25.442683250895442	25.986243812314765	24.526852560105826
84-85	24.029511302592553	25.480667714046056	25.969148800841	24.520672182520386
86-87	23.974073285288963	25.5345330270455	25.99141362855975	24.49998005910578
88-89	23.964227493688455	25.329446009538238	26.010351953168705	24.695974543604603
90-91	23.846320525957	25.421588740712224	26.04835696563964	24.68373376769113
92-93	23.93133676125334	25.265703626408275	26.19182972751604	24.61112988482234
94-95	23.84655079322843	25.199582654118302	26.207684150156684	24.746182402496583
96-97	23.83783528919369	25.09930547526793	26.240624109883846	24.82223512565453
98-99	23.755807780391304	25.039864914612547	26.2086383346444	24.995688970351743
100-101	23.856599110998584	24.90749256904875	26.297994385934093	24.937913934018578
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	13.0
1	14.5
2	29.0
3	44.5
4	39.0
5	46.5
6	78.5
7	184.5
8	315.5
9	354.0
10	329.0
11	339.5
12	412.5
13	477.5
14	550.5
15	637.5
16	726.5
17	827.0
18	921.0
19	987.5
20	1053.5
21	1139.5
22	1252.0
23	1432.0
24	1632.0
25	1993.5
26	2464.5
27	3114.0
28	4197.0
29	5861.5
30	8492.5
31	12750.5
32	18840.5
33	25169.5
34	31545.0
35	39269.0
36	49055.0
37	58696.5
38	67990.5
39	77247.0
40	85498.0
41	94067.0
42	102147.0
43	109630.0
44	116626.5
45	124086.0
46	129565.0
47	133190.5
48	136836.5
49	138785.0
50	138117.5
51	137152.0
52	136671.5
53	134942.0
54	132369.5
55	125887.0
56	116158.0
57	104835.5
58	94329.0
59	82934.5
60	69291.5
61	57723.5
62	47157.0
63	35918.0
64	25921.5
65	18958.5
66	14144.5
67	10669.0
68	7938.5
69	5829.5
70	4319.5
71	3145.0
72	2250.5
73	1624.0
74	1185.5
75	878.5
76	634.5
77	413.0
78	289.5
79	222.0
80	170.0
81	139.0
82	116.5
83	98.0
84	84.5
85	73.5
86	62.5
87	55.5
88	46.5
89	31.5
90	25.5
91	30.5
92	28.5
93	19.0
94	17.5
95	20.0
96	17.0
97	11.0
98	9.0
99	7.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05090327540025116
2	0.07223110344769818
3	0.06810729379501959
4	0.06762403485134633
5	0.06340357340993309
6	0.010535044972077297
7	0.013370164108293819
8	0.016044196929952584
9	0.00634680079357562
10-11	0.012435863483858828
12-13	0.02079624320940641
14-15	0.038241891076011475
16-17	0.025306660017023603
18-19	0.009471875295996104
20-21	0.019410900904209702
22-23	0.03484296983884281
24-25	0.03241056648902068
26-27	0.02028076700282159
28-29	0.011340476544866081
30-31	0.011340476544866081
32-33	0.007716034467316554
34-35	0.004768154910909603
36-37	6.121279953194761E-4
38-39	0.0038016370235630617
40-41	7.893229413330086E-4
42-43	0.2249087123855401
44-45	0.1953654822956475
46-47	0.20145454498593074
48-49	0.20862288598375092
50-51	0.19937653152813567
52-53	0.20651265526304427
54-55	0.20333925486625648
56-57	0.20591663589918058
58-59	0.21487303498859187
60-61	0.21891630148399158
62-63	0.20480514032873207
64-65	0.20514342158930335
66-67	0.20185726077232513
68-69	0.19704077996704816
70-71	0.18919587644808541
72-73	0.20084241699061126
74-75	0.19939264015959146
76-77	0.1938512709388046
78-79	0.21116804975376346
80-81	0.22191250693476583
82-83	0.22059159915539225
84-85	0.21495357814587074
86-87	0.23436447905008043
88-89	0.22624572879636948
90-91	0.23347850432001277
92-93	0.22879089256638208
94-95	0.23241533464393158
96-97	0.23905209080371118
98-99	0.2325442036955778
100-101	0.23898765627788807
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3103926.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.40012353092905
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.94979741614875	51.48776829872732
2	11.657276001300723	15.014600290358024
3	3.5972424737716424	6.949885790447959
4	1.6401536165023416	4.225043820498033
5	0.8643334292706766	2.783158980847155
6	0.5469347407883405	2.1133598922075474
7	0.34172897368499533	1.5405171683587913
8	0.2516420117701585	1.2964621314855769
9	0.1920392442275588	1.1130615945936937
>10	0.907133606591331	10.110809184281942
>50	0.04001705794385853	1.7264285291822619
>100	0.010748695868735962	1.1234164792312524
>500	6.017255569064052E-4	0.2605695284919851
>1k	3.510065739385193E-4	0.25491831128851267
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.665178873465411E-5
2	0.0	0.0	0.0	0.0	9.665178873465411E-5
3	0.0	0.0	0.0	0.0	9.665178873465411E-5
4	0.0	0.0	0.0	3.221726291155137E-5	9.665178873465411E-5
5	0.0	9.665178873465411E-5	0.0	3.221726291155137E-5	9.665178873465411E-5
6	0.0	9.665178873465411E-5	0.0	3.221726291155137E-5	9.665178873465411E-5
7	0.0	1.2886905164620548E-4	0.0	3.221726291155137E-5	9.665178873465411E-5
8	0.0	1.2886905164620548E-4	0.0	3.221726291155137E-5	1.2886905164620548E-4
9	0.0	1.2886905164620548E-4	0.0	6.443452582310274E-5	1.2886905164620548E-4
10-11	0.0	1.2886905164620548E-4	0.0	6.443452582310274E-5	1.2886905164620548E-4
12-13	0.0	1.2886905164620548E-4	0.0	1.1276042019042978E-4	1.7719494601353252E-4
14-15	0.0	1.2886905164620548E-4	0.0	1.2886905164620548E-4	2.2552084038085957E-4
16-17	3.221726291155137E-5	1.2886905164620548E-4	0.0	1.7719494601353252E-4	2.8995536620396233E-4
18-19	3.221726291155137E-5	1.2886905164620548E-4	0.0	2.255208403808596E-4	2.8995536620396233E-4
20-21	3.221726291155137E-5	1.2886905164620548E-4	0.0	2.5773810329241095E-4	2.8995536620396233E-4
22-23	3.221726291155137E-5	1.2886905164620548E-4	0.0	2.8995536620396233E-4	3.06063997659738E-4
24-25	3.221726291155137E-5	1.2886905164620548E-4	0.0	3.221726291155137E-4	3.221726291155137E-4
26-27	3.221726291155137E-5	1.2886905164620548E-4	0.0	4.3493304930594353E-4	3.8660715493861643E-4
28-29	3.221726291155137E-5	1.4497768310198117E-4	0.0	8.054315727887842E-4	3.8660715493861643E-4
30-31	3.221726291155137E-5	1.6108631455775686E-4	0.0	0.0035600075517264267	3.8660715493861643E-4
32-33	3.221726291155137E-5	1.6108631455775686E-4	0.0	0.012484189378226157	4.188244178501678E-4
34-35	3.221726291155137E-5	1.6108631455775686E-4	0.0	0.0234541673996094	4.510416807617192E-4
36-37	3.221726291155137E-5	1.6108631455775686E-4	0.0	0.04368660850806366	4.6715031221749486E-4
38-39	3.221726291155137E-5	1.6108631455775686E-4	0.0	0.08086532990799394	5.154762065848219E-4
40-41	3.221726291155137E-5	1.6108631455775686E-4	0.0	0.1271937539748048	5.154762065848219E-4
42-43	3.221726291155137E-5	1.9330357746930821E-4	0.0	0.17677612159568235	5.154762065848219E-4
44-45	3.221726291155137E-5	1.9330357746930821E-4	0.0	0.23602366809002534	5.154762065848219E-4
46-47	3.221726291155137E-5	1.9330357746930821E-4	0.0	0.3091407462677912	5.63802100952149E-4
48-49	3.221726291155137E-5	1.9330357746930821E-4	0.0	0.3857211802085488	5.960193638637004E-4
50-51	6.443452582310274E-5	1.9330357746930821E-4	0.0	0.4738837201660091	6.765625211425788E-4
52-53	9.665178873465411E-5	1.9330357746930821E-4	0.0	0.5652035518888014	7.248884155099059E-4
54-55	9.665178873465411E-5	2.5773810329241095E-4	0.0	0.6673967098442424	7.732143098772329E-4
56-57	9.665178873465411E-5	2.8995536620396233E-4	0.0	0.7844259173704528	7.893229413330086E-4
58-59	9.665178873465411E-5	2.8995536620396233E-4	0.0	0.9167744334111059	8.054315727887843E-4
60-61	9.665178873465411E-5	2.8995536620396233E-4	0.0	1.0645066924920248	8.376488357003356E-4
62-63	9.665178873465411E-5	2.8995536620396233E-4	0.0	1.2170393237467647	8.376488357003356E-4
64-65	9.665178873465411E-5	3.221726291155137E-4	0.0	1.3788988526143986	8.376488357003356E-4
66-67	9.665178873465411E-5	3.543898920270651E-4	0.0	1.5669026903347567	8.376488357003356E-4
68-69	9.665178873465411E-5	3.543898920270651E-4	0.0	1.772545479499189	8.376488357003356E-4
70-71	9.665178873465411E-5	3.7049852348284076E-4	0.0	2.0019323914294347	8.698660986118871E-4
72-73	9.665178873465411E-5	3.8660715493861643E-4	0.0	2.2501341849000265	9.343006244349898E-4
74-75	9.665178873465411E-5	3.8660715493861643E-4	0.0	2.5019282031852565	9.987351502580926E-4
76-77	9.665178873465411E-5	3.8660715493861643E-4	0.0	2.7744862474169807	9.987351502580926E-4
78-79	9.665178873465411E-5	4.188244178501678E-4	0.0	3.0837558627364183	0.0010470610446254194
80-81	9.665178873465411E-5	4.510416807617192E-4	0.0	3.419137569645668	0.0010631696760811952
82-83	9.665178873465411E-5	4.510416807617192E-4	0.0	3.7775224022737657	0.0010953869389927467
84-85	9.665178873465411E-5	4.6715031221749486E-4	0.0	4.153449534557202	0.0011114955704485223
86-87	1.2886905164620548E-4	4.993675751290463E-4	0.0	4.543487827995899	0.001175930096271625
88-89	1.2886905164620548E-4	5.154762065848219E-4	0.0	4.968191896327426	0.001224255990638952
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	3755	0.0	44.10905	1
TCAACGC	4215	0.0	38.62342	4
CAACGCA	4370	0.0	36.923485	5
GGTATCA	2125	0.0	36.40331	1
ATCAACG	4510	0.0	35.779606	3
AACGCAG	4625	0.0	34.872524	6
TATCAAC	4865	0.0	33.46143	2
ACGCAGA	5660	0.0	27.91213	7
CGCAGAG	5790	0.0	26.633362	8
GTACATG	7990	0.0	24.709206	1
TACATGG	8395	0.0	22.670279	2
ACATGGG	8405	0.0	21.852766	3
GCAGAGT	7420	0.0	20.335339	9
CATGGGG	6990	0.0	19.21602	4
AGAGTAC	5995	0.0	18.876757	10-11
GAGTACA	5850	0.0	18.415443	1
GTATAGC	970	0.0	18.102652	1
TACACCG	510	0.0	17.680258	5
GTATAGG	930	0.0	16.840042	1
AGTACAT	5840	0.0	16.822506	2
>>END_MODULE