FastQCFastQC Report
Mon 15 Aug 2016
SRR2921683_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921683_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3103926
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21050.043.3554151
GTATCAA37850.042.195641
TCAACGC45400.033.870774
CAACGCA45900.033.7325255
ATCAACG46200.033.2842643
TATCAAC47700.033.0335772
AACGCAG50050.031.2171846
ACGCAGA60250.025.2228897
CGCAGAG62050.023.7258518
GTACATG77750.022.253341
ACATGGG79350.019.9772383
TACATGG81150.019.8850332
GCAGAGT77450.018.7635889
ACCGTAT3306.002665E-1018.7081728
GTATAGC9350.018.3014341
AGAGTAC64100.017.92970810-11
CATGGGG70050.017.0737254
GTATATA11250.015.63303851
CAGAGTA70600.015.572638510-11
GTATTAG7350.015.5209431
Produced by FastQC (version 0.11.5)