##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921683_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3103926
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.389947118584658	33.0	31.0	34.0	28.0	34.0
2	31.533377406548997	34.0	31.0	34.0	28.0	34.0
3	31.609625036163877	34.0	31.0	34.0	28.0	34.0
4	35.09010975132784	37.0	35.0	37.0	32.0	37.0
5	34.83832185432256	37.0	35.0	37.0	32.0	37.0
6	34.813502641493386	37.0	35.0	37.0	32.0	37.0
7	34.70288756884024	37.0	35.0	37.0	32.0	37.0
8	34.64771647262209	37.0	35.0	37.0	32.0	37.0
9	36.24152089966062	39.0	37.0	39.0	32.0	39.0
10-11	36.134584233000396	39.0	37.0	39.0	32.0	39.0
12-13	36.0579612722726	39.0	37.0	39.0	32.0	39.0
14-15	37.30545944072121	40.0	38.0	41.0	32.0	41.0
16-17	37.2755144613628	40.0	38.0	41.0	31.0	41.0
18-19	37.23342518474989	40.0	38.0	41.0	31.0	41.0
20-21	37.17459050247976	40.0	38.0	41.0	31.0	41.0
22-23	37.087105813733956	40.0	38.0	41.0	31.0	41.0
24-25	37.00935025512851	40.0	37.0	41.0	31.0	41.0
26-27	36.88704176581529	40.0	37.0	41.0	30.0	41.0
28-29	36.76889461926605	40.0	37.0	41.0	30.0	41.0
30-31	36.6196206030685	40.0	37.0	41.0	30.0	41.0
32-33	36.57806355563889	40.0	37.0	41.0	30.0	41.0
34-35	36.717584278748916	40.0	37.0	41.0	30.0	41.0
36-37	36.721939730521925	40.0	37.0	41.0	30.0	41.0
38-39	36.62150370208568	40.0	37.0	41.0	30.0	41.0
40-41	36.547462149548664	40.0	37.0	41.0	30.0	41.0
42-43	36.436515400173846	40.0	37.0	41.0	30.0	41.0
44-45	36.312943349809245	40.0	36.5	41.0	29.0	41.0
46-47	36.16541808664253	40.0	36.0	41.0	28.5	41.0
48-49	36.02905401095258	40.0	36.0	41.0	28.0	41.0
50-51	35.88146012501586	40.0	35.5	41.0	28.0	41.0
52-53	35.712316917349185	39.5	35.0	41.0	28.0	41.0
54-55	35.505640276217925	39.0	35.0	41.0	27.0	41.0
56-57	35.27983350762872	39.0	35.0	41.0	27.0	41.0
58-59	35.04427199617517	39.0	35.0	40.5	26.0	41.0
60-61	34.771268548283686	38.0	35.0	40.0	26.0	41.0
62-63	34.468437230784495	37.5	34.0	40.0	26.0	41.0
64-65	34.134650600562	37.0	34.0	40.0	26.0	41.0
66-67	33.78131872344895	36.5	34.0	39.5	25.0	41.0
68-69	33.41786176603437	36.0	34.0	39.0	24.0	41.0
70-71	33.018994653867395	35.5	33.0	39.0	23.0	40.0
72-73	32.65026888527626	35.0	33.0	37.5	22.5	40.0
74-75	32.23985655585861	35.0	33.0	37.0	21.5	39.0
76-77	29.526444573743056	33.0	29.0	34.5	17.0	36.5
78-79	31.095531755589533	34.5	31.5	36.0	19.0	37.5
80-81	31.274610767138135	35.0	32.0	36.0	20.0	37.0
82-83	31.094200699372344	35.0	32.0	35.0	19.5	37.0
84-85	30.893539343399297	35.0	32.0	35.0	18.5	36.5
86-87	30.67976314512653	35.0	32.0	35.0	17.5	36.0
88-89	30.412915127486933	35.0	32.0	35.0	10.5	36.0
90-91	30.220664571255888	34.5	32.0	35.0	7.5	35.0
92-93	30.06507500500978	34.0	31.0	35.0	4.5	35.0
94-95	29.961707205648587	34.0	31.0	35.0	2.0	35.0
96-97	29.760619132028275	34.0	31.0	35.0	2.0	35.0
98-99	29.561111798412718	34.0	31.0	35.0	2.0	35.0
100-101	28.4521380342186	33.0	29.0	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	55846.0
3	19086.0
4	10506.0
5	7334.0
6	6537.0
7	6428.0
8	6627.0
9	7240.0
10	8130.0
11	8572.0
12	9177.0
13	10108.0
14	10720.0
15	11355.0
16	11961.0
17	12824.0
18	12132.0
19	11390.0
20	10694.0
21	10840.0
22	11717.0
23	12916.0
24	15671.0
25	18605.0
26	22348.0
27	27123.0
28	32827.0
29	39609.0
30	48766.0
31	60255.0
32	75905.0
33	100997.0
34	142469.0
35	215894.0
36	382382.0
37	751641.0
38	803049.0
39	94242.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.579311697883305	19.39126579386561	13.937808084445633	26.09161442380545
2	14.700701048996109	20.580652851877627	38.22453542617078	26.49411067295549
3	17.84306485381937	24.645237538285862	28.4179214892516	29.09377611864316
4	12.799051463883778	17.051676531141563	35.74473295035329	34.404539054621374
5	14.33500473707616	37.06349615555656	34.336100387344594	14.265398720022688
6	33.78807878316716	35.76110528145429	16.92250393944832	13.528311995930231
7	29.292354263600355	31.14855186624939	20.834549535008247	18.72454433514201
8	26.492738551112367	35.42645668743391	19.356228209048798	18.724576552404923
9	26.146411398471294	16.05119324725952	18.981869729455447	38.82052562481375
10-11	25.588515006776095	25.490527884669156	27.711354435053476	21.20960267350127
12-13	26.318617822084576	22.494622110864576	27.578430838750954	23.60832922829989
14-15	23.300195745408857	24.170925322747948	24.701875280001637	27.827003651841554
16-17	23.849167151536474	27.231609258725886	25.30490417619492	23.61431941354272
18-19	23.80376757417046	26.670308344667266	26.440450912978125	23.085473168184148
20-21	24.356575613510596	25.832596496139796	26.400088662093204	23.410739228256404
22-23	24.01847273718393	25.712666106193595	26.187210356691875	24.081650799930603
24-25	23.951394162260662	25.83245889776981	26.205843151872788	24.010303788096742
26-27	23.895932135677096	26.02767861745451	26.08786213206866	23.988527114799734
28-29	24.005833530136307	26.12835763863494	25.9475781313179	23.91823069991085
30-31	24.2013277169986	25.939797596060508	25.88872536107023	23.970149325870665
32-33	23.93474452862841	25.969422795208057	25.841361276703584	24.25447139945995
34-35	24.182497854931853	26.01443277245265	25.91086053722237	23.892208835393124
36-37	24.32312831911582	25.858648798682648	25.848145547990995	23.970077334210536
38-39	24.151352859467792	25.969260537986493	25.82061006296704	24.058776539578673
40-41	24.368159864374057	25.756202576751118	25.9098997335873	23.965737825287523
42-43	24.214404582968847	25.762397037051354	25.813804002687146	24.209394377292654
44-45	24.18646873326111	25.84237552305889	25.868102569885497	24.103053173794496
46-47	24.333909233328097	25.826867547028478	25.669497839368198	24.169725380275224
48-49	24.115269893972382	25.886204166193437	25.7373482543664	24.26117768546778
50-51	24.190386348340144	25.962922905275597	25.723172813695992	24.123517932688266
52-53	24.351748680155527	25.863216865270918	25.65647492589492	24.12855952867864
54-55	24.053285641264637	25.72947262557629	25.9125707338471	24.304670999311973
56-57	24.05320702338637	25.863521075572027	25.825630669062093	24.257641231979505
58-59	24.153999257927296	25.760475493637042	25.81903898201674	24.266486266418923
60-61	24.137221093553787	25.814924832615453	25.901998143187832	24.145855930642927
62-63	24.107873936330133	25.818993977035333	25.881685527838368	24.191446558796166
64-65	23.907316315368448	25.709611877723855	25.8718586049102	24.5112132019975
66-67	23.926353174983742	25.850677081108696	25.784834640376967	24.43813510353059
68-69	24.085039296983208	25.726074740177047	25.80093611367454	24.38794984916521
70-71	23.953954375093595	25.569642371543438	25.788174199381558	24.68822905398141
72-73	23.96839213909315	25.69733981028709	25.722461478765503	24.611806571854256
74-75	23.94925994417537	25.792329986315345	25.673739301259175	24.584670768250117
76-77	23.979109880587988	25.519132996756667	25.829614237028398	24.67214288562695
78-79	23.888045087120272	25.534621998930472	25.88193585841886	24.695397055530396
80-81	23.97008526756167	25.661412761216333	25.701001617404657	24.667500353817342
82-83	23.93722356050047	25.687632429122726	25.716007508680903	24.6591365016959
84-85	23.883469890795933	25.681377094899137	25.720524749408273	24.714628264896657
86-87	23.83433845784836	25.693339258820814	25.7129243167273	24.759397966603526
88-89	23.727407996200135	25.548630060633087	25.77915856852282	24.944803374643964
90-91	23.607441797943345	25.6438028962729	25.83861757513403	24.910137730649723
92-93	23.71220586204101	25.506974633257794	25.907477542423894	24.8733419622773
94-95	23.582997973340895	25.428900996574978	25.918070130782343	25.070030899301788
96-97	23.51194753254888	25.397794609029972	25.939121362235884	25.151136496185256
98-99	23.40584049005437	25.316903682962778	25.919129817466764	25.35812600951609
100-101	23.379922307857733	25.274769706346902	25.945696043789823	25.399611942005546
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	15.0
1	10.5
2	18.5
3	29.0
4	28.5
5	30.0
6	58.0
7	166.5
8	258.0
9	265.0
10	233.5
11	193.0
12	200.0
13	237.5
14	271.5
15	305.5
16	339.5
17	381.0
18	421.0
19	510.0
20	612.0
21	708.0
22	856.0
23	1059.0
24	1341.0
25	1779.0
26	2370.5
27	3210.0
28	4452.0
29	6416.5
30	9422.0
31	13862.0
32	20132.5
33	26467.0
34	32710.0
35	40388.0
36	49727.0
37	59356.0
38	68515.0
39	77305.5
40	85790.5
41	94261.0
42	102324.5
43	109667.0
44	117008.5
45	124562.5
46	130202.0
47	134331.5
48	137823.5
49	140255.5
50	139502.0
51	137937.5
52	137360.0
53	135838.0
54	132832.0
55	126033.0
56	116582.5
57	104901.5
58	93599.5
59	82279.5
60	68601.0
61	56371.5
62	45861.0
63	34663.0
64	24702.5
65	17962.0
66	13188.0
67	9824.0
68	7264.5
69	5321.5
70	3874.0
71	2703.5
72	1863.5
73	1309.5
74	952.0
75	653.5
76	418.0
77	257.5
78	141.0
79	84.0
80	49.0
81	31.0
82	18.0
83	12.0
84	13.5
85	9.5
86	7.5
87	6.5
88	4.5
89	3.0
90	2.5
91	2.5
92	1.5
93	1.0
94	0.5
95	0.5
96	0.5
97	1.0
98	1.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.15908884425724065
2	1.9330357746930821E-4
3	0.009665178873465411
4	0.006314583530664069
5	0.024034078132017325
6	0.0020941220892508393
7	0.0
8	0.0
9	3.221726291155137E-5
10-11	0.04760100595181715
12-13	4.188244178501678E-4
14-15	0.00481648080527693
16-17	0.0
18-19	0.0014658854624755874
20-21	2.094122089250839E-4
22-23	1.6108631455775684E-5
24-25	6.121279953194761E-4
26-27	0.002770684610393418
28-29	0.004671503122174949
30-31	0.015415960303177332
32-33	0.010937760758471691
34-35	0.009504092558907655
36-37	0.004027157863943922
38-39	1.6108631455775684E-5
40-41	0.00306063997659738
42-43	0.008408705619914908
44-45	0.006072954058827434
46-47	0.002851227767672296
48-49	0.008167076148078272
50-51	0.0022229911408970446
52-53	0.0018202753545026524
54-55	0.004043266495399697
56-57	0.007667708572949226
58-59	0.0147071805191232
60-61	0.007071689209085526
62-63	0.02052239647465822
64-65	0.040078275061969905
66-67	0.05874817891921392
68-69	0.049131325940115836
70-71	0.06731797085368658
72-73	0.03308712901016326
74-75	0.026273177904370143
76-77	0.22421604123294175
78-79	0.08223456358173488
80-81	0.06581986812829944
82-83	0.027416890737730217
84-85	0.0505488855082241
86-87	0.06673806012127867
88-89	0.292564964499798
90-91	0.2960766461571571
92-93	0.050790514980060736
94-95	0.009536309821819206
96-97	0.014175595681082603
98-99	8.2154020424456E-4
100-101	2.738467347481866E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3103926.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.39907837905372
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.14926573734562	50.17990502126525
2	12.044733789376505	15.272500431350352
3	3.798532321383974	7.224703451063743
4	1.7036276654285212	4.3203369555690845
5	0.9202429785537369	2.9171278362551107
6	0.5757820563808572	2.1902431033045615
7	0.34526323342807064	1.5322559558258237
8	0.2509649387988618	1.2728756660242766
9	0.19561714102975708	1.1161751810788776
>10	0.9627799503005642	10.577782877966623
>50	0.04101234759975292	1.7383743776982725
>100	0.011160688533990144	1.1383449588306207
>500	8.137214722190705E-4	0.35367615005738323
>1k	2.0343036755774164E-4	0.16569803370998396
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.443452582310274E-5
2	0.0	0.0	0.0	3.221726291155137E-5	6.443452582310274E-5
3	0.0	0.0	0.0	3.221726291155137E-5	6.443452582310274E-5
4	0.0	0.0	0.0	3.221726291155137E-5	6.443452582310274E-5
5	0.0	3.221726291155137E-5	0.0	6.443452582310274E-5	6.443452582310274E-5
6	3.221726291155137E-5	6.443452582310274E-5	0.0	6.443452582310274E-5	6.443452582310274E-5
7	3.221726291155137E-5	6.443452582310274E-5	0.0	6.443452582310274E-5	6.443452582310274E-5
8	3.221726291155137E-5	6.443452582310274E-5	0.0	6.443452582310274E-5	6.443452582310274E-5
9	3.221726291155137E-5	6.443452582310274E-5	0.0	6.443452582310274E-5	6.443452582310274E-5
10-11	3.221726291155137E-5	6.443452582310274E-5	0.0	9.665178873465411E-5	6.443452582310274E-5
12-13	3.221726291155137E-5	6.443452582310274E-5	0.0	1.2886905164620548E-4	1.1276042019042978E-4
14-15	3.221726291155137E-5	6.443452582310274E-5	0.0	1.2886905164620548E-4	1.2886905164620548E-4
16-17	3.221726291155137E-5	6.443452582310274E-5	0.0	1.7719494601353252E-4	1.6108631455775686E-4
18-19	3.221726291155137E-5	9.665178873465411E-5	0.0	2.255208403808596E-4	1.6108631455775686E-4
20-21	3.221726291155137E-5	9.665178873465411E-5	0.0	2.255208403808596E-4	2.5773810329241095E-4
22-23	3.221726291155137E-5	9.665178873465411E-5	0.0	2.416294718366353E-4	2.5773810329241095E-4
24-25	3.221726291155137E-5	9.665178873465411E-5	0.0	2.7384673474818667E-4	2.8995536620396233E-4
26-27	3.221726291155137E-5	9.665178873465411E-5	0.0	4.188244178501678E-4	3.3828126057128944E-4
28-29	3.221726291155137E-5	9.665178873465411E-5	0.0	8.376488357003356E-4	3.543898920270651E-4
30-31	3.221726291155137E-5	9.665178873465411E-5	0.0	0.0037533111291957345	3.543898920270651E-4
32-33	3.221726291155137E-5	9.665178873465411E-5	0.0	0.012790253375885893	3.8660715493861643E-4
34-35	3.221726291155137E-5	9.665178873465411E-5	0.0	0.02377634002872491	4.188244178501678E-4
36-37	3.221726291155137E-5	9.665178873465411E-5	0.0	0.044347062397750464	4.188244178501678E-4
38-39	3.221726291155137E-5	9.665178873465411E-5	0.0	0.08168687011223849	4.6715031221749486E-4
40-41	3.221726291155137E-5	9.665178873465411E-5	0.0	0.12862742217436884	4.832589436732706E-4
42-43	3.221726291155137E-5	9.665178873465411E-5	0.0	0.17885413505347741	4.832589436732706E-4
44-45	3.221726291155137E-5	9.665178873465411E-5	0.0	0.23877824406896297	4.832589436732706E-4
46-47	3.221726291155137E-5	1.2886905164620548E-4	0.0	0.31274907971388494	4.993675751290463E-4
48-49	3.221726291155137E-5	1.2886905164620548E-4	0.0	0.3901671624903429	5.476934694963733E-4
50-51	3.221726291155137E-5	1.2886905164620548E-4	0.0	0.47889350454875534	5.476934694963733E-4
52-53	3.221726291155137E-5	1.4497768310198117E-4	0.0	0.5705032916377517	6.282366267752517E-4
54-55	3.221726291155137E-5	1.9330357746930824E-4	0.0	0.6731474912739543	6.765625211425788E-4
56-57	3.221726291155137E-5	2.255208403808596E-4	0.0	0.7905310886921917	6.765625211425788E-4
58-59	3.221726291155137E-5	2.416294718366353E-4	0.0	0.9230890169417698	6.765625211425788E-4
60-61	3.221726291155137E-5	2.5773810329241095E-4	0.0	1.0708373846541446	6.765625211425788E-4
62-63	3.221726291155137E-5	2.5773810329241095E-4	0.0	1.2232733641201499	7.087797840541302E-4
64-65	3.221726291155137E-5	2.5773810329241095E-4	0.0	1.384987915304682	7.087797840541302E-4
66-67	3.221726291155137E-5	2.5773810329241095E-4	0.0	1.5740549227011211	7.409970469656815E-4
68-69	3.221726291155137E-5	2.5773810329241095E-4	0.0	1.7815179872200562	7.409970469656815E-4
70-71	3.221726291155137E-5	2.8995536620396233E-4	0.0	2.011468701251254	7.732143098772329E-4
72-73	3.221726291155137E-5	3.221726291155137E-4	0.0	2.26110416292141	7.893229413330086E-4
74-75	3.221726291155137E-5	3.221726291155137E-4	0.0	2.514621804772408	8.054315727887843E-4
76-77	3.221726291155137E-5	3.221726291155137E-4	0.0	2.788839038044077	8.376488357003356E-4
78-79	3.221726291155137E-5	3.3828126057128944E-4	0.0	3.100653817133527	8.376488357003356E-4
80-81	3.221726291155137E-5	3.543898920270651E-4	0.0	3.439386119385578	8.537574671561113E-4
82-83	3.221726291155137E-5	3.543898920270651E-4	0.0	3.8012987423024907	9.020833615234384E-4
84-85	3.221726291155137E-5	3.543898920270651E-4	0.0	4.180189862773791	9.020833615234384E-4
86-87	3.221726291155137E-5	3.543898920270651E-4	0.0	4.573337122083451	9.181919929792141E-4
88-89	3.221726291155137E-5	3.543898920270651E-4	0.0	5.0021005655418325	9.343006244349897E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2105	0.0	43.355415	1
GTATCAA	3785	0.0	42.19564	1
TCAACGC	4540	0.0	33.87077	4
CAACGCA	4590	0.0	33.732525	5
ATCAACG	4620	0.0	33.284264	3
TATCAAC	4770	0.0	33.033577	2
AACGCAG	5005	0.0	31.217184	6
ACGCAGA	6025	0.0	25.222889	7
CGCAGAG	6205	0.0	23.725851	8
GTACATG	7775	0.0	22.25334	1
ACATGGG	7935	0.0	19.977238	3
TACATGG	8115	0.0	19.885033	2
GCAGAGT	7745	0.0	18.763588	9
ACCGTAT	330	6.002665E-10	18.708172	8
GTATAGC	935	0.0	18.301434	1
AGAGTAC	6410	0.0	17.929708	10-11
CATGGGG	7005	0.0	17.073725	4
GTATATA	1125	0.0	15.6330385	1
CAGAGTA	7060	0.0	15.5726385	10-11
GTATTAG	735	0.0	15.520943	1
>>END_MODULE