FastQCFastQC Report
Mon 15 Aug 2016
SRR2921682_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921682_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3593289
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA26500.043.6975331
GTATCAA48750.041.373971
TCAACGC56350.035.375174
CAACGCA58100.034.141525
ATCAACG58800.034.0621643
TATCAAC61950.032.484772
AACGCAG63550.031.3450166
ACGCAGA71850.027.3978547
GTACATG90300.027.2241881
TACATGG92400.025.991652
CGCAGAG74650.025.8004768
ACATGGG97050.024.6474573
GTATAGC12400.021.432821
CATGGGG85950.021.2597434
GCAGAGT88000.020.3784379
AGAGTAC73150.017.80310610-11
GTATTAA18550.017.6529831
TAAGGCG7600.017.483455
TAGCCCT26750.016.6781274
CAGAGTA81000.016.28274310-11
Produced by FastQC (version 0.11.5)