##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2921682_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3593289
Sequences flagged as poor quality	0
Sequence length	101
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.230410913232973	31.0	31.0	34.0	26.0	34.0
2	30.29632378581294	31.0	31.0	34.0	26.0	34.0
3	30.3574769521739	31.0	31.0	34.0	26.0	34.0
4	33.80730856883485	37.0	35.0	37.0	28.0	37.0
5	33.80542561424923	37.0	35.0	37.0	28.0	37.0
6	33.83800384550199	37.0	35.0	37.0	29.0	37.0
7	33.754894471332534	37.0	35.0	37.0	29.0	37.0
8	33.722232472812514	37.0	35.0	37.0	29.0	37.0
9	35.258415340374796	39.0	35.0	39.0	29.0	39.0
10-11	35.154143599359806	39.0	35.0	39.0	28.0	39.0
12-13	35.04864095262029	39.0	35.0	39.0	27.0	39.0
14-15	36.16769177207845	40.0	36.0	41.0	27.0	41.0
16-17	36.10587250287968	40.0	36.0	41.0	27.0	41.0
18-19	36.074917853810256	40.0	36.0	41.0	27.0	41.0
20-21	36.00181658085392	40.0	36.0	41.0	27.0	41.0
22-23	35.89993930351831	40.0	36.0	41.0	27.0	41.0
24-25	35.792929959154414	40.0	36.0	41.0	27.0	41.0
26-27	35.648255817998496	40.0	36.0	41.0	26.5	41.0
28-29	35.512194816503765	40.0	36.0	41.0	25.5	41.0
30-31	35.3704372233906	39.5	35.5	41.0	25.0	41.0
32-33	35.196442312321665	39.0	35.0	41.0	24.5	41.0
34-35	35.02791940754	39.0	35.0	41.0	24.0	41.0
36-37	35.00066165009271	39.0	35.0	41.0	24.0	41.0
38-39	35.18281371189459	39.5	35.0	41.0	24.0	41.0
40-41	35.250281983998505	40.0	35.0	41.0	24.0	41.0
42-43	35.10139429364017	40.0	35.0	41.0	23.5	41.0
44-45	34.988725371101516	40.0	35.0	41.0	23.0	41.0
46-47	34.843855448309334	40.0	35.0	41.0	22.5	41.0
48-49	34.69189466808821	39.0	35.0	41.0	21.0	41.0
50-51	33.02162099959118	37.0	32.5	39.5	17.0	40.0
52-53	33.14595569685601	37.5	32.5	39.5	16.5	40.0
54-55	34.06366214907846	39.0	34.0	40.5	18.0	41.0
56-57	34.0956119866785	39.0	34.0	41.0	18.0	41.0
58-59	33.917991288760795	38.5	34.0	41.0	16.0	41.0
60-61	33.675711026861464	38.0	34.0	40.0	13.5	41.0
62-63	33.39520506143536	37.5	33.5	40.0	12.5	41.0
64-65	33.11140517781898	37.0	33.0	40.0	9.0	41.0
66-67	32.778315771428346	36.5	33.0	39.5	7.5	41.0
68-69	32.420650690773826	36.0	33.0	39.0	4.5	41.0
70-71	32.006452584248024	35.5	32.0	39.0	2.0	40.5
72-73	31.64106992229125	35.0	32.0	38.0	2.0	40.0
74-75	31.23274832611571	35.0	32.0	37.0	2.0	39.0
76-77	30.850068558359766	35.0	31.5	37.0	2.0	39.0
78-79	30.49771073242369	35.0	31.0	36.0	2.0	38.5
80-81	30.175324612075457	35.0	31.0	36.0	2.0	37.0
82-83	29.86152268854523	35.0	31.0	35.0	2.0	37.0
84-85	29.597104769474427	35.0	31.0	35.0	2.0	36.5
86-87	29.361840781523558	35.0	30.5	35.0	2.0	36.0
88-89	29.14193264165504	34.5	30.0	35.0	2.0	36.0
90-91	28.92573864779593	34.0	30.0	35.0	2.0	35.5
92-93	28.73832914636145	34.0	30.0	35.0	2.0	35.0
94-95	28.516642273972398	34.0	30.0	35.0	2.0	35.0
96-97	28.28852062831573	34.0	29.5	35.0	2.0	35.0
98-99	28.031186748407933	34.0	29.0	35.0	2.0	35.0
100-101	26.975076872469764	33.0	26.5	34.5	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	131354.0
3	22044.0
4	14719.0
5	11212.0
6	10267.0
7	10542.0
8	11111.0
9	12048.0
10	13078.0
11	14138.0
12	14040.0
13	13949.0
14	15172.0
15	15397.0
16	15308.0
17	15998.0
18	15493.0
19	15062.0
20	15165.0
21	15918.0
22	17919.0
23	20139.0
24	23846.0
25	28026.0
26	33399.0
27	39286.0
28	45593.0
29	54629.0
30	65078.0
31	78661.0
32	98268.0
33	126071.0
34	173524.0
35	248848.0
36	411246.0
37	748296.0
38	837993.0
39	140439.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.75921325293891	18.107623603080114	13.443009678405609	24.69015346557536
2	15.67355853427032	19.738299161790852	39.28088807390144	25.307254230037383
3	18.662758395009295	24.198959805607583	29.43594171012414	27.702340089258982
4	13.306257448166056	16.42435671100127	37.12156214656459	33.147823694268084
5	14.458560092502704	37.14285952990046	34.37164943659015	14.026930941006691
6	32.94185362986186	35.577828718259305	17.710272362104437	13.770045289774396
7	28.685976655221403	31.705778745652356	21.454472782980847	18.153771816145394
8	26.400660024851135	34.36744278225861	20.432434298133494	18.799462894756765
9	26.70466958915486	15.716811617516782	19.573221354406567	38.00529743892179
10-11	25.30416137771539	25.330965070724314	28.284534422218282	21.08033912934201
12-13	26.071249573930285	22.774830452436284	27.78916824649398	23.364751727139456
14-15	23.384581503745856	24.31374721742383	25.15563007157612	27.146041207254196
16-17	23.59620515861251	27.108604786143488	25.904914614676894	23.39027544056711
18-19	23.53547688580222	26.804411796427225	26.92527625914398	22.734835058626572
20-21	24.305748472791386	25.95405653218843	26.6962412268664	23.043953768153784
22-23	24.006763033114	25.818975831455372	26.363899457094814	23.810361678335813
24-25	23.87944166022522	25.93960612983876	26.38309529546526	23.79785691447076
26-27	23.75147077776482	26.15056087885635	26.3333033633803	23.764664979998535
28-29	23.81656158316348	26.328128283424583	26.192511975845406	23.662798157566527
30-31	24.212505968420498	26.01955791034378	26.075028907960313	23.69290721327541
32-33	23.882703312824425	26.060916091785913	26.022048757977856	24.034331837411806
34-35	24.203313516653164	26.159868592616796	26.014186631547513	23.622631259182526
36-37	24.256247658815127	26.013409527711	25.992634555860878	23.73770825761299
38-39	24.0456200430958	26.102682668377327	26.01905148883677	23.832645799690106
40-41	24.323669568310148	25.905384845088754	26.028685443905815	23.742260142695294
42-43	24.078408474951228	25.912929439291055	25.941844904308365	24.066817181449355
44-45	24.063339917297235	25.92673881835859	26.06669627475	23.943224989594174
46-47	24.160322476776937	25.834971064692958	25.882732903109567	24.121973555420535
48-49	23.934850829049807	25.903570435414668	26.0095742304091	24.152004505126424
50-51	24.037090796620184	25.90143378497116	26.068617360178457	23.992858058230198
52-53	24.184765896453147	25.793387981411342	26.083530701506664	23.938315420628847
54-55	23.829715252640433	25.706680753262884	26.264255043625727	24.199348950470952
56-57	23.845896172632077	25.832181418979978	26.172121664468822	24.14980074391912
58-59	24.000163458354802	25.704704950696105	26.19633524137025	24.098796349578844
60-61	24.028796344596536	25.717398035611172	26.237715595997578	24.01609002379471
62-63	23.968405154801633	25.73886559221508	26.261430051640055	24.031299201343227
64-65	23.81336743079624	25.598101304216797	26.234528087943115	24.354003177043843
66-67	23.811172652417927	25.67577291453016	26.123536356428012	24.3895180766239
68-69	23.89721205792998	25.659572741355284	26.166883292636655	24.276331908078078
70-71	23.818237641659362	25.41301831382727	26.217735798229718	24.55100824628365
72-73	23.75229602409229	25.596868649851373	26.12980003603356	24.521035290022773
74-75	23.80551901649508	25.623395571907455	26.102745591409153	24.468339820188312
76-77	23.697746362530232	25.401548830206234	26.247523915842557	24.653180891420973
78-79	23.669869481629313	25.419458582714284	26.318310855053273	24.59236108060313
80-81	23.772868814444674	25.47917022634312	26.165027181398788	24.582933777813423
82-83	23.716161762404457	25.487978343751106	26.28065808975214	24.515201804092293
84-85	23.725006478420095	25.468085029228902	26.255676811495967	24.551231680855036
86-87	23.661429663720547	25.500204857058318	26.25546268051241	24.582902798708727
88-89	23.628776441735315	25.28680012894376	26.301332525784215	24.783090903536706
90-91	23.562257366522903	25.336023258496887	26.355054218629704	24.746665156350506
92-93	23.627575101369203	25.17514678796191	26.497535725425188	24.699742385243702
94-95	23.49333261538277	25.17558285472675	26.464880708840404	24.866203821050078
96-97	23.45491537870669	25.0787870738697	26.531008276444346	24.935289270979265
98-99	23.320857082314937	24.97167838474515	26.511724193153068	25.195740339786845
100-101	23.49395346449332	24.80453025248783	26.64818542550777	25.05333085751108
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	38.0
1	37.5
2	49.0
3	62.5
4	60.0
5	83.5
6	141.0
7	284.5
8	440.0
9	490.5
10	474.0
11	469.5
12	517.0
13	605.5
14	733.0
15	836.0
16	922.5
17	1042.0
18	1148.5
19	1216.5
20	1331.0
21	1453.5
22	1559.5
23	1663.0
24	1823.5
25	2142.0
26	2537.5
27	3249.0
28	4241.5
29	5920.5
30	8977.5
31	13558.0
32	20508.0
33	28401.5
34	36814.0
35	46590.5
36	58632.5
37	71452.0
38	83807.5
39	95252.5
40	105488.5
41	115164.0
42	124506.5
43	134274.0
44	143624.5
45	153100.0
46	159552.5
47	163642.0
48	167730.0
49	168436.0
50	165186.5
51	160958.0
52	157387.0
53	154132.0
54	148761.0
55	138977.5
56	126502.0
57	112361.5
58	99873.5
59	87089.0
60	71359.5
61	57853.0
62	46744.0
63	35226.0
64	25167.0
65	18345.0
66	13591.5
67	10356.0
68	7757.5
69	5672.5
70	4206.5
71	2997.5
72	2046.5
73	1451.0
74	1081.5
75	749.0
76	510.0
77	376.5
78	271.5
79	180.5
80	137.0
81	129.0
82	106.0
83	89.0
84	83.5
85	82.0
86	73.5
87	55.5
88	45.0
89	42.0
90	32.5
91	31.5
92	30.0
93	18.5
94	21.5
95	22.5
96	15.0
97	9.0
98	6.5
99	9.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.055520165508535495
2	0.07728295720160555
3	0.07274672312747457
4	0.07347029420678382
5	0.06887840081885982
6	0.011827604181016334
7	0.014944525753425345
8	0.019397270856866787
9	0.006456480399990093
10-11	0.013928743276702765
12-13	0.023947419759446014
14-15	0.04225933399734895
16-17	0.029054161799955416
18-19	0.011730200381878553
20-21	0.021901939977552597
22-23	0.03922590139562946
24-25	0.03669340261804714
26-27	0.022319384831000235
28-29	0.012022411779291897
30-31	0.011702370724982043
32-33	0.008279322926711434
34-35	0.005231975496543696
36-37	6.400821086197075E-4
38-39	0.003659599881890936
40-41	0.0010575269620673427
42-43	0.24217367431342152
44-45	0.21029480233847042
46-47	0.2168069420522535
48-49	0.22375044144793255
50-51	0.2145527398436363
52-53	0.22220589549017625
54-55	0.21839323249535453
56-57	0.22132926129793626
58-59	0.23037389978930164
60-61	0.23541106768756978
62-63	0.21968731154104218
64-65	0.21931161117293932
66-67	0.2171269831065634
68-69	0.21193675209536444
70-71	0.20343479191348093
72-73	0.21548503334966937
74-75	0.21441359155915377
76-77	0.20833281152726651
78-79	0.22713174476085835
80-81	0.23868105237290962
82-83	0.23794356646515213
84-85	0.23126444880998995
86-87	0.2527628587625432
88-89	0.24435830237979744
90-91	0.25187230974185487
92-93	0.2467655677013455
94-95	0.2508147827797875
96-97	0.25775828217546654
98-99	0.2508147827797875
100-101	0.2586627460246031
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3593289.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.24935142224122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.7059979162511	50.41352672665392
2	11.695208023287298	14.79428644442227
3	3.6372116429042345	6.901538321973518
4	1.616450602047956	4.089578087424983
5	0.8958769006387252	2.83318164597835
6	0.556708122052133	2.1126856590774787
7	0.3615577590928743	1.6007805635012264
8	0.2679885641371036	1.3560082296199598
9	0.1949807553135266	1.1099165682059344
>10	1.0130244067879837	11.197207538848927
>50	0.04216443179598637	1.7844298481636216
>100	0.01181633884167605	1.2492614175273289
>500	7.498750677335968E-4	0.33147368967407803
>1k	2.646617817248551E-4	0.22612525892841967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	2.782965689650902E-5
6	0.0	0.0	0.0	0.0	5.565931379301804E-5
7	0.0	0.0	0.0	0.0	5.565931379301804E-5
8	0.0	0.0	0.0	2.782965689650902E-5	8.348897068952706E-5
9	0.0	0.0	0.0	2.782965689650902E-5	8.348897068952706E-5
10-11	0.0	0.0	0.0	2.782965689650902E-5	8.348897068952706E-5
12-13	0.0	0.0	0.0	5.565931379301804E-5	1.6697794137905412E-4
14-15	0.0	0.0	0.0	8.348897068952706E-5	2.0872242672381766E-4
16-17	0.0	0.0	0.0	8.348897068952706E-5	2.2263725517207217E-4
18-19	0.0	0.0	0.0	1.1131862758603608E-4	2.5046691206858117E-4
20-21	0.0	0.0	0.0	1.6697794137905412E-4	2.5046691206858117E-4
22-23	0.0	0.0	0.0	2.643817405168357E-4	2.922113974133447E-4
24-25	0.0	0.0	0.0	4.452745103441443E-4	3.3395588275810824E-4
26-27	0.0	0.0	0.0	6.81826593964471E-4	3.3395588275810824E-4
28-29	0.0	0.0	0.0	0.0015167163008597416	3.3395588275810824E-4
30-31	2.782965689650902E-5	0.0	0.0	0.007096562508609801	3.3395588275810824E-4
32-33	2.782965689650902E-5	0.0	0.0	0.024016993901687284	3.3395588275810824E-4
34-35	2.782965689650902E-5	0.0	0.0	0.04770003192061646	4.174448534476353E-4
36-37	2.782965689650902E-5	0.0	0.0	0.08547879115762745	4.174448534476353E-4
38-39	2.782965689650902E-5	1.391482844825451E-5	0.0	0.151880352512698	4.174448534476353E-4
40-41	2.782965689650902E-5	2.782965689650902E-5	0.0	0.22671429990741074	4.4527451034414434E-4
42-43	2.782965689650902E-5	2.782965689650902E-5	0.0	0.3080603870159066	4.4527451034414434E-4
44-45	2.782965689650902E-5	2.782965689650902E-5	0.0	0.4124216003778154	4.4527451034414434E-4
46-47	2.782965689650902E-5	2.782965689650902E-5	0.0	0.5339954565302151	4.4527451034414434E-4
48-49	2.782965689650902E-5	2.782965689650902E-5	0.0	0.6619701337688118	5.009338241371623E-4
50-51	2.782965689650902E-5	2.782965689650902E-5	0.0	0.7951072123617109	5.009338241371623E-4
52-53	2.782965689650902E-5	2.782965689650902E-5	0.0	0.9332814588528782	5.565931379301804E-4
54-55	4.174448534476353E-5	4.174448534476353E-5	0.0	1.0912982507112563	6.122524517231984E-4
56-57	5.565931379301804E-5	5.565931379301804E-5	0.0	1.2749879010566643	6.122524517231984E-4
58-59	5.565931379301804E-5	5.565931379301804E-5	0.0	1.469224991365849	6.122524517231984E-4
60-61	5.565931379301804E-5	5.565931379301804E-5	0.0	1.679450219562078	6.122524517231984E-4
62-63	5.565931379301804E-5	5.565931379301804E-5	0.0	1.902282282332426	6.679117655162165E-4
64-65	5.565931379301804E-5	5.565931379301804E-5	0.0	2.1451795277251566	6.679117655162165E-4
66-67	5.565931379301804E-5	5.565931379301804E-5	0.0	2.419830411636804	6.81826593964471E-4
68-69	5.565931379301804E-5	5.565931379301804E-5	0.0	2.7227979714406496	6.957414224127255E-4
70-71	5.565931379301804E-5	5.565931379301804E-5	0.0	3.038344536161717	7.0965625086098E-4
72-73	5.565931379301804E-5	5.565931379301804E-5	0.0	3.3687521376655205	8.348897068952706E-4
74-75	5.565931379301804E-5	5.565931379301804E-5	0.0	3.7189883697080863	8.348897068952706E-4
76-77	5.565931379301804E-5	5.565931379301804E-5	0.0	4.116535018474718	8.348897068952706E-4
78-79	5.565931379301804E-5	5.565931379301804E-5	0.0	4.561127702224898	8.348897068952706E-4
80-81	5.565931379301804E-5	5.565931379301804E-5	0.0	5.020428359644882	8.766341922400342E-4
82-83	5.565931379301804E-5	5.565931379301804E-5	0.0	5.500865641477766	0.0010575269620673427
84-85	5.565931379301804E-5	5.565931379301804E-5	0.0	6.005946084492509	0.0010575269620673427
86-87	5.565931379301804E-5	5.565931379301804E-5	0.0	6.5456744503434034	0.0010714417905155972
88-89	5.565931379301804E-5	5.565931379301804E-5	0.0	7.139434095058872	0.0010992714474121063
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	2650	0.0	43.697533	1
GTATCAA	4875	0.0	41.37397	1
TCAACGC	5635	0.0	35.37517	4
CAACGCA	5810	0.0	34.14152	5
ATCAACG	5880	0.0	34.062164	3
TATCAAC	6195	0.0	32.48477	2
AACGCAG	6355	0.0	31.345016	6
ACGCAGA	7185	0.0	27.397854	7
GTACATG	9030	0.0	27.224188	1
TACATGG	9240	0.0	25.99165	2
CGCAGAG	7465	0.0	25.800476	8
ACATGGG	9705	0.0	24.647457	3
GTATAGC	1240	0.0	21.43282	1
CATGGGG	8595	0.0	21.259743	4
GCAGAGT	8800	0.0	20.378437	9
AGAGTAC	7315	0.0	17.803106	10-11
GTATTAA	1855	0.0	17.652983	1
TAAGGCG	760	0.0	17.48345	5
TAGCCCT	2675	0.0	16.678127	4
CAGAGTA	8100	0.0	16.282743	10-11
>>END_MODULE