FastQCFastQC Report
Mon 15 Aug 2016
SRR2921682_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2921682_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3593289
Sequences flagged as poor quality0
Sequence length101
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA46400.043.845451
GGTATCA25750.041.164841
TCAACGC58200.033.92094
CAACGCA58950.033.2770085
ATCAACG61100.032.3109053
TATCAAC62400.031.865922
AACGCAG64350.030.6999786
ACGCAGA77350.024.7414957
GTACATG92350.023.1619131
CGCAGAG80650.023.022568
TACATGG93900.022.0352442
GTATTAA19000.020.014061
ACATGGG98150.019.9690193
GCAGAGT90700.019.5293969
TAAGGCG7250.018.9993175
CATGGGG80450.018.4045644
AGAGTAC78450.018.19025410-11
ATGGGGG45050.016.9749725
GTATATA11900.016.7764911
TAAGGTG20600.016.3707715
Produced by FastQC (version 0.11.5)